FastQCFastQC Report
Thu 26 May 2016
SRR935931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401397
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT66741.6626930445419372No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT36770.9160506929548552RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT21140.5266606377227533RNA PCR Primer, Index 26 (100% over 24bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA8450.21051477714083563No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC7620.18983699429741627No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT7510.18709656524587878No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT7280.1813665772290276No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA6720.16741530205756394No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC6640.16542226274735486RNA PCR Primer, Index 26 (95% over 22bp)
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC4870.12132626800897865No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG4720.11758931930233658RNA PCR Primer, Index 26 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT4180.10413630395842519RNA PCR Primer, Index 26 (100% over 21bp)
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA4110.10239239456199224No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAC152.681288E-4119.212682
TATAGTT208.4175996E-489.409512
TAGGGTC304.257352E-579.3463755
CTTTACA551.9299478E-976.014411
ATAGTTT250.002041357371.527613
GTATAGT300.004171546559.725611
GTAGACC300.00420470559.606343
TAGGGAG401.7691473E-459.5097775
CTTTCGA150.004237510359.49495338-39
TGGACAG453.1658687E-452.8975835
TATTAGG803.7847713E-852.155552
GTTTAAG350.007676836651.1933821
TAGACCA350.00773775451.091154
CTATAAT350.00773775451.091153
AGACCGC350.0077951950.9956748
TACAGTG350.0077951950.9956747
TGTCTTG302.193891E-549.5791316-17
GTATTAG856.006303E-849.18581
GTCAGAT1251.0913936E-1147.685073
GTACCAT505.3306593E-447.595969
Produced by FastQC (version 0.11.5)