##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935931_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 401397 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35532652212149 33.0 31.0 34.0 31.0 34.0 2 32.45352606023463 34.0 31.0 34.0 31.0 34.0 3 32.46306524463312 34.0 31.0 34.0 30.0 34.0 4 35.93420728107086 37.0 35.0 37.0 35.0 37.0 5 35.9236541379233 37.0 35.0 37.0 35.0 37.0 6 36.01385162320595 37.0 35.0 37.0 35.0 37.0 7 35.990769736694595 37.0 35.0 37.0 35.0 37.0 8 36.02369972869752 37.0 35.0 37.0 35.0 37.0 9 37.685839704830876 39.0 37.0 39.0 35.0 39.0 10-11 37.63031612094759 39.0 37.0 39.0 35.0 39.0 12-13 37.60062980042203 39.0 37.0 39.0 35.0 39.0 14-15 38.756460561489 40.0 38.0 41.0 34.0 41.0 16-17 38.68387282416162 40.0 38.0 41.0 34.5 41.0 18-19 38.754572156742576 40.0 38.0 41.0 34.5 41.0 20-21 38.848287356407745 40.0 38.0 41.0 35.0 41.0 22-23 38.66365593165868 40.0 38.0 41.0 34.0 41.0 24-25 38.3576758172084 40.0 38.0 41.0 34.0 41.0 26-27 38.198328587408476 40.0 37.5 41.0 33.0 41.0 28-29 38.12776876757923 40.0 37.0 41.0 33.0 41.0 30-31 37.962052531533615 40.0 37.0 41.0 33.0 41.0 32-33 37.58056985976477 40.0 35.5 41.0 33.0 41.0 34-35 37.36854286404731 40.0 35.0 41.0 33.0 41.0 36-37 37.24638076517762 40.0 35.0 41.0 33.0 41.0 38-39 36.805753406228746 39.5 35.0 41.0 31.5 41.0 40-41 36.51951185484695 39.0 35.0 41.0 31.0 41.0 42-43 36.1754995677596 39.0 35.0 41.0 30.5 41.0 44-45 35.69315789604805 39.0 35.0 41.0 29.5 41.0 46-47 35.128813867567516 38.0 35.0 41.0 27.5 41.0 48-49 34.400837574770115 37.5 35.0 40.0 25.0 41.0 50-51 33.64598763817368 37.0 34.0 40.0 21.0 41.0 52-53 33.14718969000765 36.0 34.0 39.0 18.0 41.0 54-55 32.647050177255934 35.5 34.0 39.0 13.0 41.0 56-57 32.29572219025055 35.0 33.0 37.5 12.5 40.5 58-59 31.999156695241865 35.0 33.0 37.0 10.5 39.5 60-61 31.59323188763244 35.0 33.0 36.5 7.5 39.0 62-63 31.244413884508354 35.0 33.0 36.0 4.5 38.5 64-65 30.943636848307285 35.0 33.0 35.0 2.0 37.0 66-67 30.48743637844827 35.0 33.0 35.0 2.0 36.5 68-69 30.001732698550313 35.0 32.0 35.0 2.0 36.0 70-71 29.52261352227346 35.0 31.5 35.0 2.0 35.5 72-73 29.235815165534373 35.0 31.0 35.0 2.0 35.0 74-75 29.132705276820705 35.0 31.0 35.0 2.0 35.0 76-77 29.025711452751267 35.0 31.0 35.0 2.0 35.0 78-79 29.085838957441137 35.0 31.0 35.0 2.0 35.0 80-81 29.064608106189134 35.0 31.0 35.0 2.0 35.0 82-83 28.982903459667114 35.0 31.0 35.0 2.0 35.0 84-85 28.930410541184912 35.0 31.0 35.0 2.0 35.0 86-87 28.904867251125445 35.0 31.0 35.0 2.0 35.0 88-89 28.825059728896825 35.0 31.0 35.0 2.0 35.0 90-91 28.730767793481267 35.0 31.0 35.0 2.0 35.0 92-93 28.649352137659225 35.0 31.0 35.0 2.0 35.0 94-95 28.6097691313089 35.0 31.0 35.0 2.0 35.0 96-97 28.566776782088557 35.0 30.5 35.0 2.0 35.0 98-99 28.481697670884436 35.0 30.0 35.0 2.0 35.0 100-101 28.37317792609312 35.0 30.0 35.0 2.0 35.0 102-103 27.623656878352357 33.5 28.0 35.0 2.0 35.0 104-105 28.014997620809325 34.0 29.0 35.0 2.0 35.0 106-107 27.83301320139413 35.0 29.0 35.0 2.0 35.0 108-109 27.478982154824276 35.0 29.0 35.0 2.0 35.0 110-111 26.98107360045043 34.0 26.0 35.0 2.0 35.0 112-113 26.325427195519648 34.0 24.0 35.0 2.0 35.0 114-115 25.528297421256262 34.0 9.5 35.0 2.0 35.0 116-117 24.725852460282464 34.0 2.0 35.0 2.0 35.0 118-119 24.034673901399366 34.0 2.0 35.0 2.0 35.0 120-121 23.282816015067375 33.5 2.0 35.0 2.0 35.0 122-123 22.469761856715422 33.0 2.0 35.0 2.0 35.0 124-125 20.276873020974246 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08928888735479745 1101 2 0.19562033688947622 1101 3 0.21331335763656512 1101 4 0.2674421513764571 1101 5 0.2987789769510698 1101 6 0.23914785614061174 1101 7 0.2297610753489323 1101 8 0.262398685556434 1101 9 0.37102367051304697 1101 10-11 0.32998577107478866 1101 12-13 0.2105443109121623 1101 14-15 0.28382217727425996 1101 16-17 0.2390070929499899 1101 18-19 0.4164396512693571 1101 20-21 0.31252338430155646 1101 22-23 0.4230556576609672 1101 24-25 0.3601533866046225 1101 26-27 0.5341595450140773 1101 28-29 0.4425851499443638 1101 30-31 0.506881733599549 1101 32-33 0.5175143673077116 1101 34-35 0.8166372487438878 1101 36-37 0.5604641620585582 1101 38-39 0.6966606772047541 1101 40-41 0.7428215192762764 1101 42-43 0.6897413000827015 1101 44-45 0.5413408004288698 1101 46-47 0.4083703216440142 1101 48-49 0.17416864025694423 1101 50-51 0.16341821736325812 1101 52-53 0.1951995219251259 1101 54-55 0.28872328746013665 1101 56-57 0.03175829510753658 1101 58-59 0.1282924760259263 1101 60-61 0.16916948348682936 1101 62-63 0.0781569864214795 1101 64-65 0.020517758852655277 1101 66-67 -0.08769067254234741 1101 68-69 0.09149584172676839 1101 70-71 0.049399457125147705 1101 72-73 0.006859599082265078 1101 74-75 0.03925167656872475 1101 76-77 0.16632243729715412 1101 78-79 0.16889945637828774 1101 80-81 0.15728092975347252 1101 82-83 -0.020431234063078563 1101 84-85 0.136924117321378 1101 86-87 0.10410960257851087 1101 88-89 0.0904191539104211 1101 90-91 0.11302886459114703 1101 92-93 0.21497270822595738 1101 94-95 -0.03743927540701364 1101 96-97 0.13712700582636117 1101 98-99 0.180668182947624 1101 100-101 0.29389409338972783 1101 102-103 0.16771889868015677 1101 104-105 0.15506789492068407 1101 106-107 0.19469223371597622 1101 108-109 0.1196876924206407 1101 110-111 0.15362074589339159 1101 112-113 0.11746143056410219 1101 114-115 0.415006062088505 1101 116-117 0.2763820431577457 1101 118-119 -0.27566186678091853 1101 120-121 -0.03867177031096247 1101 122-123 -0.21899591726505818 1101 124-125 -0.25602424463772167 1102 1 0.17987115258391384 1102 2 0.12532902252150535 1102 3 0.10673679219443244 1102 4 0.12742659328442585 1102 5 0.2403597954230321 1102 6 0.2052908306442376 1102 7 0.26072822160227105 1102 8 0.19604778515002863 1102 9 0.26220063290463 1102 10-11 0.36916090139197877 1102 12-13 0.32483449113884433 1102 14-15 0.27508710831701677 1102 16-17 0.4058421844446656 1102 18-19 0.34893461669986436 1102 20-21 0.4057628844069754 1102 22-23 0.4882251608925401 1102 24-25 0.24005073227779405 1102 26-27 0.16398600685676 1102 28-29 0.33379482795001536 1102 30-31 0.19051084031788434 1102 32-33 0.30500282596608486 1102 34-35 0.439044536328673 1102 36-37 0.4502888790684949 1102 38-39 0.6413585666841399 1102 40-41 0.5728202833530815 1102 42-43 0.4507864549002605 1102 44-45 0.5930460109356659 1102 46-47 0.7532419673848096 1102 48-49 0.7081219950614113 1102 50-51 0.7321846206086491 1102 52-53 0.9207609582132861 1102 54-55 0.6609797197133744 1102 56-57 0.37422352567990913 1102 58-59 0.3112999860768966 1102 60-61 0.44630708535961716 1102 62-63 0.3330170726452977 1102 64-65 0.32211028224431715 1102 66-67 0.5517032888823081 1102 68-69 0.6908804246753881 1102 70-71 0.499571759359263 1102 72-73 0.1889238089274876 1102 74-75 0.2463960396684186 1102 76-77 0.22425997228468475 1102 78-79 0.33190318595828927 1102 80-81 0.4203126493588485 1102 82-83 0.4579237521600099 1102 84-85 0.4331476449369944 1102 86-87 0.2313878815918109 1102 88-89 0.2523214578166666 1102 90-91 0.3571000683076413 1102 92-93 0.26223223028718934 1102 94-95 0.09130938870541527 1102 96-97 0.17011525001570504 1102 98-99 0.10082754433883778 1102 100-101 0.1340746835794029 1102 102-103 0.3075926891112033 1102 104-105 0.3525702395396948 1102 106-107 0.36075305567760196 1102 108-109 0.07816976062905923 1102 110-111 -0.19726875530254517 1102 112-113 -0.4222062514982916 1102 114-115 -0.3709247698332092 1102 116-117 -0.27129914681819045 1102 118-119 -0.6034141586914359 1102 120-121 -0.48353505539482455 1102 122-123 0.009030095283197426 1102 124-125 0.32043214544872 1103 1 0.15264459767286098 1103 2 0.045175821111605785 1103 3 0.13637793454407188 1103 4 0.20101600240435147 1103 5 0.1855111928308233 1103 6 0.2669946412517703 1103 7 0.13500315152372622 1103 8 0.19395930578503595 1103 9 0.31079073587152095 1103 10-11 0.22667248863629652 1103 12-13 0.07393595931902297 1103 14-15 0.22125152229386913 1103 16-17 0.25931211311719693 1103 18-19 0.27003380906199226 1103 20-21 0.2088734003515782 1103 22-23 0.25820101501815884 1103 24-25 0.22764127930674505 1103 26-27 0.3463359277582043 1103 28-29 0.36710295151029015 1103 30-31 0.33761333539157334 1103 32-33 0.2969993012235008 1103 34-35 0.5198950539385123 1103 36-37 0.33521782913927467 1103 38-39 0.43800936738353613 1103 40-41 0.12845640225069843 1103 42-43 0.3349625810138832 1103 44-45 0.5409901423780354 1103 46-47 0.4598071473687071 1103 48-49 0.3736738809374671 1103 50-51 0.5686969294538073 1103 52-53 0.14388132678478627 1103 54-55 0.23134620968034625 1103 56-57 0.31060122821384084 1103 58-59 0.11281118476691177 1103 60-61 0.11525188289506971 1103 62-63 0.18242105810226406 1103 64-65 0.2908594149356105 1103 66-67 0.2949313425027995 1103 68-69 0.4056121834386772 1103 70-71 0.5224929938517846 1103 72-73 0.6298184552043047 1103 74-75 0.6158626101216385 1103 76-77 0.6574286326049652 1103 78-79 0.6263197994300249 1103 80-81 0.7323217526310302 1103 82-83 0.8116053041569025 1103 84-85 0.8320818959985772 1103 86-87 0.8378135983357247 1103 88-89 0.7945038840607346 1103 90-91 0.7465667775302585 1103 92-93 0.6319208317692429 1103 94-95 0.7484761340080688 1103 96-97 0.6884015867812678 1103 98-99 0.8264076004016694 1103 100-101 0.8208860404665295 1103 102-103 0.7859307868603622 1103 104-105 0.6311359190934098 1103 106-107 0.7051544045161009 1103 108-109 0.7523149411680379 1103 110-111 0.5254595897336714 1103 112-113 0.3111171366952128 1103 114-115 0.6373354782864666 1103 116-117 0.8572776342869695 1103 118-119 0.824737988071071 1103 120-121 1.012756332576643 1103 122-123 0.4400929300643597 1103 124-125 0.4668477320269453 1104 1 0.11458035355575902 1104 2 0.17145055825883304 1104 3 0.2272498942795096 1104 4 0.2535470308899548 1104 5 0.26353037647202626 1104 6 0.3413905410119753 1104 7 0.30855186163466186 1104 8 0.34487896111192384 1104 9 0.3845805590552658 1104 10-11 0.2624705333805579 1104 12-13 0.3709628901999409 1104 14-15 0.43187630819073775 1104 16-17 0.4080281530655441 1104 18-19 0.3690647453185534 1104 20-21 0.12802476138875818 1104 22-23 0.22940578984753301 1104 24-25 0.24469377080849597 1104 26-27 0.27901531324926054 1104 28-29 0.23985651550406573 1104 30-31 0.25131988892393764 1104 32-33 0.1900696061063698 1104 34-35 0.24201223752701395 1104 36-37 0.17718006793344188 1104 38-39 0.3330889478731436 1104 40-41 0.03521613241486676 1104 42-43 -0.10773024356647198 1104 44-45 -0.07201349408142477 1104 46-47 0.1321832310599831 1104 48-49 -0.297766736733621 1104 50-51 -0.10926230530863279 1104 52-53 0.11066569527934433 1104 54-55 -0.013314387436842878 1104 56-57 0.04273185089164855 1104 58-59 -0.0756061648338715 1104 60-61 -0.231549086827453 1104 62-63 -0.13529152984471438 1104 64-65 -0.3821490964876162 1104 66-67 -0.38675328686819554 1104 68-69 -0.3657454280925876 1104 70-71 -0.34349491096784135 1104 72-73 -0.35882090288155055 1104 74-75 -0.5412561545361925 1104 76-77 -0.4120593644437065 1104 78-79 -0.36603507158107007 1104 80-81 -0.27804083926862333 1104 82-83 -0.2989773198911614 1104 84-85 -0.24200437768452332 1104 86-87 -0.2217630703511979 1104 88-89 -0.21163922906477595 1104 90-91 -0.40873705129263627 1104 92-93 -0.38122839482434046 1104 94-95 -0.4695236815338397 1104 96-97 -0.36052262729552 1104 98-99 -0.2699223424163222 1104 100-101 -0.3366806938347686 1104 102-103 -0.25750250518949613 1104 104-105 -0.21739105131424097 1104 106-107 -0.19081452747156646 1104 108-109 -0.3126175998738816 1104 110-111 -0.40300031698323835 1104 112-113 -0.43056577985068145 1104 114-115 -0.13456562582695142 1104 116-117 -0.5743938195062803 1104 118-119 -0.3456551711689606 1104 120-121 -0.49050132902181787 1104 122-123 -0.35786946683674614 1104 124-125 -0.0916452453901151 1105 1 -0.08906661852945774 1105 2 -0.0061375334684257155 1105 3 0.05857902429425366 1105 4 0.17109927576715478 1105 5 0.09644210678168008 1105 6 0.031169104087176436 1105 7 0.02787865270600065 1105 8 -0.10293336410290266 1105 9 0.06712619387501206 1105 10-11 -0.004359689835993663 1105 12-13 -0.08743188277000513 1105 14-15 -0.23980404468464656 1105 16-17 -0.03418151287232263 1105 18-19 -0.07319894512737335 1105 20-21 0.10241714456870454 1105 22-23 0.1293714683953482 1105 24-25 0.1222837692831007 1105 26-27 -0.003990483262661826 1105 28-29 0.08984634618489906 1105 30-31 0.22049731812006002 1105 32-33 0.39829594972766813 1105 34-35 0.46240045128610063 1105 36-37 0.48074060080576686 1105 38-39 0.3730098814166425 1105 40-41 0.29069167520227523 1105 42-43 0.27010445415051265 1105 44-45 0.40217550271523805 1105 46-47 0.45068948519126195 1105 48-49 0.6053164737204426 1105 50-51 0.5112473046979744 1105 52-53 0.6330159520546559 1105 54-55 0.680630418583398 1105 56-57 0.5529742620372247 1105 58-59 0.6132031350085043 1105 60-61 0.4792322982338213 1105 62-63 0.6148578320305056 1105 64-65 0.716935029205171 1105 66-67 0.7332923479515507 1105 68-69 0.657771122474351 1105 70-71 0.6776731624546386 1105 72-73 0.6784909647139159 1105 74-75 0.7353239034289274 1105 76-77 0.6269336187260706 1105 78-79 0.7482998549455999 1105 80-81 0.6754504151168952 1105 82-83 0.7378801062541598 1105 84-85 0.7244305132495299 1105 86-87 0.7453518938019528 1105 88-89 0.5700127768334262 1105 90-91 0.6310249866389235 1105 92-93 0.8151093791116928 1105 94-95 0.7060941046053735 1105 96-97 0.7696573696536859 1105 98-99 0.8788325515685251 1105 100-101 0.6293251789470489 1105 102-103 0.6318108115339527 1105 104-105 0.6529437735192332 1105 106-107 0.4996568204716887 1105 108-109 0.41532768918901297 1105 110-111 0.3093986254075709 1105 112-113 0.4467254948734798 1105 114-115 0.782093625711159 1105 116-117 0.6941556542314551 1105 118-119 0.41541985298317385 1105 120-121 0.3918364703375161 1105 122-123 0.1412597852098294 1105 124-125 0.05337877962641002 1106 1 -0.09932767984685853 1106 2 -0.029463516301454717 1106 3 0.0841279406882407 1106 4 -0.005858091331056414 1106 5 0.11318820627425907 1106 6 0.1530510692790017 1106 7 0.022970415403399613 1106 8 0.1406586594186976 1106 9 0.07691302936607514 1106 10-11 -0.08076932610478593 1106 12-13 0.10632493422624378 1106 14-15 0.14368756151823447 1106 16-17 0.09092815185002223 1106 18-19 0.2505905934508945 1106 20-21 0.2716268472291148 1106 22-23 0.13900418049285435 1106 24-25 0.031110890258105428 1106 26-27 -0.01774896004931037 1106 28-29 -0.005722013467959641 1106 30-31 0.03483494319711866 1106 32-33 0.008840953758578962 1106 34-35 -0.07061159404988615 1106 36-37 -0.2670364656332751 1106 38-39 -0.3077235088237913 1106 40-41 -0.2021089839716268 1106 42-43 -0.1598701747677751 1106 44-45 -0.28947693717663725 1106 46-47 -0.214201798538042 1106 48-49 -0.1512960126455667 1106 50-51 -0.23240103314050486 1106 52-53 -0.3455875714432324 1106 54-55 -0.36824151673219774 1106 56-57 -0.3132436684412703 1106 58-59 -0.4157038930182395 1106 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2216 34-35 -1.1606113939308145 2216 36-37 -0.993933969648161 2216 38-39 -1.3782954991580425 2216 40-41 -1.448162886043356 2216 42-43 -1.5197868251747693 2216 44-45 -1.6446219984881196 2216 46-47 -2.0624702682775364 2216 48-49 -2.259006350296467 2216 50-51 -2.0259481937010335 2216 52-53 -1.8125779307069934 2216 54-55 -2.2999183644565946 2216 56-57 -2.1029585487950833 2216 58-59 -1.5996458384757872 2216 60-61 -1.7071768326286225 2216 62-63 -1.6204756024545617 2216 64-65 -1.903132515737589 2216 66-67 -2.058625018057281 2216 68-69 -2.1650947503256006 2216 70-71 -2.24178694888203 2216 72-73 -1.7906311603096299 2216 74-75 -1.9163252186437774 2216 76-77 -2.1163563588810277 2216 78-79 -2.0554718839573596 2216 80-81 -2.057081290719907 2216 82-83 -2.0007638675440553 2216 84-85 -2.3048607618901613 2216 86-87 -2.613693164063818 2216 88-89 -2.321125284820301 2216 90-91 -2.215243560752409 2216 92-93 -2.5199077818032762 2216 94-95 -2.5900546303003438 2216 96-97 -2.7453531989585827 2216 98-99 -2.7536688087479035 2216 100-101 -2.7384245960775395 2216 102-103 -2.675994788784152 2216 104-105 -2.709253556074973 2216 106-107 -2.876779359759773 2216 108-109 -2.5064959719457 2216 110-111 -2.7667267748393805 2216 112-113 -2.508482162661629 2216 114-115 -2.561934323559502 2216 116-117 -2.172377683985065 2216 118-119 -1.782920822570894 2216 120-121 -2.106137442978934 2216 122-123 -1.9387817567611627 2216 124-125 -2.419021184796083 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 10.0 4 24.0 5 75.0 6 235.0 7 528.0 8 900.0 9 1213.0 10 1961.0 11 3704.0 12 5457.0 13 5286.0 14 6959.0 15 9210.0 16 4493.0 17 1783.0 18 1769.0 19 2281.0 20 3268.0 21 5729.0 22 5720.0 23 1899.0 24 2401.0 25 3116.0 26 4012.0 27 5096.0 28 6291.0 29 7852.0 30 10019.0 31 13872.0 32 18797.0 33 25306.0 34 34682.0 35 49759.0 36 110128.0 37 44860.0 38 2695.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.49421531748983 19.94947919136753 11.326745326700397 16.229560164442237 2 19.152408095745844 21.229160230794527 36.93634216584121 22.68208950761841 3 29.61905544884491 22.319299845290324 27.94415503852794 20.117489667336827 4 15.749060448042215 22.118811239211052 27.89877029045685 34.23335802228989 5 15.67122891662347 33.59508951712588 27.904322341401173 22.829359224849476 6 31.76271574919281 35.287449675130546 9.648423486267788 23.30141108940886 7 27.4695625527844 39.764372927550525 12.192417980204137 20.57364653946093 8 35.76957476015018 27.17110491608059 13.09775608686662 23.961564236902618 9 33.899854757260265 13.874543158020614 17.736555081378285 34.489047003340836 10-11 29.088408732501737 24.93952371343084 18.761475546653315 27.210592007414107 12-13 32.39025204473377 25.585268449938592 20.25513394469814 21.769345560629503 14-15 34.810549157068934 19.212898950415674 23.817068886912455 22.15948300560293 16-17 30.09302510980401 21.275943766395862 25.45248220589591 23.17854891790422 18-19 28.904550856134946 21.355042514019786 25.71220014100753 24.028206488837732 20-21 34.532644738251655 18.921292386340703 24.1444255936143 22.401637281793338 22-23 30.47220083857129 22.004524199234172 24.007404141037426 23.515870821157108 24-25 25.784696945916387 22.799248624180052 25.43491854697469 25.981135882928868 26-27 23.456702466635278 21.691866157445123 28.523506653014348 26.327924722905255 28-29 24.429803909844868 19.237064552051958 31.40220280669761 24.930928731405565 30-31 26.197878907914106 20.118237057078154 27.584162313121425 26.09972172188631 32-33 24.28381378037205 19.474609924837505 30.322224630478058 25.91935166431239 34-35 22.77296541827667 21.091961325072187 29.448027763037594 26.687045493613553 36-37 23.578028734644256 21.68040618141142 27.30376659516638 27.437798488777943 38-39 24.747444549909442 17.75760655909237 29.603858524104567 27.89109036689362 40-41 22.8259055249541 18.290620009616415 30.736278547173995 28.14719591825549 42-43 24.055486214396222 18.142138581005838 32.0464527637227 25.755922440875246 44-45 23.800128052775683 18.418548220340462 31.630530372673437 26.15079335421042 46-47 22.155372362025624 18.135785768204546 30.461612817235807 29.247229052534024 48-49 21.08585764218467 19.135917807058846 30.801426019626454 28.976798531130026 50-51 21.409228270266095 19.679768408832153 29.707621133192326 29.203382187709426 52-53 20.718017324494202 19.89302361502453 29.43968191092609 29.949277149555183 54-55 19.10863808150036 20.10690164600134 27.71009748453526 33.07436278796304 56-57 19.88168820394771 20.53827009170472 26.949503857776712 32.63053784657085 58-59 19.775434295722192 20.559695264289466 27.09748702655974 32.5673834134286 60-61 20.5332874934292 22.36227475541671 24.147290587622727 32.95714716353137 62-63 18.90472524707459 23.35331355241818 24.27932944192408 33.46263175858315 64-65 18.163439188633696 23.06046133877433 24.41734741415606 34.35875205843592 66-67 18.92490477009046 23.807975645059628 23.721403000022423 33.54571658482749 68-69 19.12607717546469 23.359292670348808 23.97688572659985 33.53774442758665 70-71 18.783274414108725 22.74406634827864 24.74694629008189 33.72571294753075 72-73 19.016086318532523 21.361644456734854 25.571441739724015 34.050827485008604 74-75 19.18611748468474 20.599805180407426 25.710331666654213 34.50374566825363 76-77 19.02144261167871 20.650752247774648 26.080040458697994 34.24776468184864 78-79 19.44695650440835 21.18326743847114 26.352837714282867 33.01693834283764 80-81 19.22149393244095 21.760252318776672 25.778966957899534 33.23928679088284 82-83 18.143384230574718 21.20656108540921 26.31135758363914 34.33869710037693 84-85 18.680632889632957 20.156727628756567 26.69427026111306 34.468369220497415 86-87 19.259735374205587 20.18737060815103 26.38759133725464 34.16530268038874 88-89 19.21813067860497 20.223992705476125 26.347107726266017 34.21076888965289 90-91 19.126450870335354 19.483080341905893 26.323814079327946 35.066654708430804 92-93 19.072514244002818 19.164318617229327 26.47316746263674 35.28999967613111 94-95 18.758859682558665 18.883175509532958 26.823444121405988 35.53452068650239 96-97 18.722486715147348 19.093441156760015 26.64992513646091 35.53414699163173 98-99 19.26334775795534 19.711158777967945 26.518633671900886 34.506859792175824 100-101 19.682882532754356 20.47200153464027 25.957593106077027 33.88752282652835 102-103 19.085593564476067 21.669195335291494 25.68915562398324 33.5560554762492 104-105 18.531404071281052 23.42431557784438 25.630360964332073 32.4139193865425 106-107 18.253522389956068 25.518533420196736 24.832802478347467 31.395141711499726 108-109 17.87968021684268 27.11330677608452 24.39230985782156 30.614703149251238 110-111 17.624043329214334 28.576678160003187 23.990388448200264 29.808890062582215 112-113 17.59907921354473 29.655626862250745 23.56176942470777 29.183524499496755 114-115 17.24344968646688 30.488822675034193 23.501157219981415 28.76657041851751 116-117 16.89490606535873 31.019671400044096 23.699857612499205 28.385564922097966 118-119 16.710307010441294 31.35178767623266 23.94933069581227 27.988574617513773 120-121 17.115705372146355 31.74022065573893 23.689661916144843 27.454412055969872 122-123 17.158171934247832 32.09279845335911 23.624889756992022 27.124139855401037 124-125 16.317381683345957 33.34184741437742 23.61237091822495 26.72839998405167 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.5 3 0.5 4 0.0 5 0.0 6 1.0 7 2.0 8 2.0 9 4.0 10 7.0 11 8.5 12 6.5 13 6.0 14 10.0 15 15.0 16 20.0 17 26.0 18 27.0 19 37.0 20 68.0 21 102.5 22 150.0 23 240.5 24 357.0 25 475.0 26 629.0 27 824.5 28 1071.0 29 1364.0 30 1680.5 31 1936.5 32 2207.0 33 2542.5 34 2745.5 35 2865.0 36 2874.5 37 2782.0 38 2674.0 39 2480.5 40 2388.5 41 2333.5 42 2387.5 43 2586.5 44 2794.5 45 3175.5 46 3733.0 47 4316.5 48 5304.5 49 7151.0 50 10569.5 51 16732.0 52 26303.5 53 37520.0 54 44351.0 55 44588.0 56 40715.0 57 33708.0 58 24628.0 59 16101.0 60 10455.5 61 6878.5 62 4565.5 63 3564.0 64 2996.0 65 2453.0 66 2035.0 67 1747.0 68 1362.0 69 1121.5 70 889.5 71 502.5 72 336.0 73 236.5 74 160.5 75 117.0 76 87.0 77 84.5 78 56.5 79 34.5 80 29.0 81 26.5 82 14.5 83 5.5 84 3.0 85 0.5 86 0.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19183003360762535 2 2.4912991377613685E-4 3 0.0 4 0.10164500482066383 5 0.033134278532226204 6 0.0012456495688806842 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 1.2456495688806842E-4 108-109 0.0 110-111 0.0012456495688806842 112-113 2.4912991377613685E-4 114-115 9.965196551045474E-4 116-117 0.006851072628843763 118-119 0.0024912991377613685 120-121 6.228247844403421E-4 122-123 0.002740429051537505 124-125 0.02516212129138982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 401397.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.73507780327533 #Duplication Level Percentage of deduplicated Percentage of total 1 82.72167176746981 50.24107170814284 2 8.814087990274768 10.706486397085053 3 3.005813066526209 5.476748713727127 4 1.5089345921437622 3.665810394156197 5 0.9673020383591819 2.937458227950586 6 0.666347551787314 2.4282402241094734 7 0.4616176699445372 1.9625469569613696 8 0.329158342581106 1.59931660370085 9 0.2612657774424444 1.428119758827007 >10 1.2146315681253688 12.065999532657075 >50 0.02827254032439835 1.1435123235058215 >100 0.017209372370345716 2.1421880479385305 >500 0.002458481767192245 1.100459519979575 >1k 8.194939223974151E-4 1.4411490457263045 >5k 4.0974696119870755E-4 1.660892545532267 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 6674 1.6626930445419372 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 3677 0.9160506929548552 RNA PCR Primer, Index 26 (100% over 22bp) ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 2114 0.5266606377227533 RNA PCR Primer, Index 26 (100% over 24bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 845 0.21051477714083563 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 762 0.18983699429741627 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 751 0.18709656524587878 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 728 0.1813665772290276 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 672 0.16741530205756394 No Hit TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC 664 0.16542226274735486 RNA PCR Primer, Index 26 (95% over 22bp) TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 487 0.12132626800897865 No Hit CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG 472 0.11758931930233658 RNA PCR Primer, Index 26 (96% over 27bp) TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT 418 0.10413630395842519 RNA PCR Primer, Index 26 (100% over 21bp) GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 411 0.10239239456199224 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 2.4912991377613685E-4 0.0 0.0 0.0 0.0 5 7.473897413284105E-4 0.0 0.0 0.0 0.0 6 7.473897413284105E-4 0.0 0.0 0.0 0.0 7 7.473897413284105E-4 0.0 0.0 0.0 0.0 8 7.473897413284105E-4 0.0 0.0 0.0 0.0 9 7.473897413284105E-4 0.0 0.0 0.0 0.0 10-11 0.0034878187928659155 0.0 0.0 3.736948706642053E-4 0.0 12-13 0.00548085810307501 0.0 0.0 0.0012456495688806842 0.0 14-15 0.005729988016851147 0.0 0.0 0.0016193444395448896 0.0 16-17 0.005979117930627284 0.0 0.0 0.0021176042670971633 0.0 18-19 0.005979117930627284 0.0 0.0 0.0032386888790897793 0.0 20-21 0.0062282478444034214 0.0 0.0 0.006726507671955694 0.0 22-23 0.0062282478444034214 0.0 0.0 0.024414731550061408 0.0 24-25 0.006726507671955695 0.0 0.0 0.056926185297847265 0.0 26-27 0.007224767499507968 0.0 0.0 0.13328450387023322 0.0 28-29 0.008345852111500584 0.0 0.0 0.4285034516949554 0.0 30-31 0.008968676895940926 0.0 0.0 1.0492106318682004 0.0 32-33 0.008968676895940926 0.0 0.0 1.987932146976684 0.0 34-35 0.0094669367234932 0.0 0.0 3.357648412917884 0.0 36-37 0.01108628116303809 0.0 0.0 5.201458904775073 0.0 38-39 0.011459976033702295 0.0 0.0 7.616275159007167 0.0 40-41 0.011584540990590364 0.0 0.0 10.421104293255805 0.0 42-43 0.012954755516359117 0.0 0.0 12.687688248791098 0.0 44-45 0.013702145257687526 0.0 0.0 14.676616915422885 0.0 46-47 0.013702145257687526 0.0 0.0 16.98617578108456 0.0 48-49 0.013702145257687526 0.0 0.0 19.445710854839472 0.0 50-51 0.013826710214575596 0.0 0.0 21.79849376054131 0.0 52-53 0.0142004050852398 0.0 0.0 23.84933121074647 0.0 54-55 0.0142004050852398 0.0 0.0 25.884099781513065 0.0 56-57 0.0142004050852398 0.0 0.0 27.93443897189067 0.0 58-59 0.0142004050852398 0.0 0.0 29.929346756453086 0.0 60-61 0.0142004050852398 0.0 0.0 32.01020436126827 0.0 62-63 0.0142004050852398 0.0 0.0 34.022551239795014 0.0 64-65 0.0142004050852398 0.0 0.0 35.894762541822686 0.0 66-67 0.0142004050852398 0.0 0.0 37.8844385981958 0.0 68-69 0.0142004050852398 0.0 0.0 39.97563509443269 0.0 70-71 0.0142004050852398 0.0 0.0 42.25741597470834 0.0 72-73 0.0142004050852398 0.0 0.0 44.442908143309495 0.0 74-75 0.0142004050852398 0.0 0.0 46.354481971713795 0.0 76-77 0.0142004050852398 0.0 0.0 48.261446896713224 0.0 78-79 0.0142004050852398 0.0 0.0 50.19668806692626 0.0 80-81 0.0142004050852398 0.0 0.0 52.06852567408326 0.0 82-83 0.0142004050852398 0.0 0.0 53.98981556912483 0.0 84-85 0.0142004050852398 0.0 0.0 56.00166418782402 0.0 86-87 0.0142004050852398 0.0 0.0 57.65862724434911 0.0 88-89 0.0142004050852398 0.0 0.0 59.13870806209314 0.0 90-91 0.0142004050852398 0.0 0.0 60.849856874864535 0.0 92-93 0.0142004050852398 0.0 0.0 62.37453194717449 0.0 94-95 0.0142004050852398 0.0 0.0 63.753964279752964 0.0 96-97 0.0142004050852398 0.0 0.0 65.04321158354446 0.0 98-99 0.0142004050852398 0.0 0.0 66.24077907906636 0.0 100-101 0.0142004050852398 0.0 0.0 67.46151565656943 0.0 102-103 0.0142004050852398 0.0 0.0 68.59543045912152 0.0 104-105 0.0142004050852398 0.0 0.0 69.66183105504027 0.0 106-107 0.0142004050852398 0.0 0.0 70.67479328445404 0.0 108-109 0.0142004050852398 0.0 0.0 71.5991898295204 0.0 110-111 0.0142004050852398 0.0 0.0 72.50913683958774 0.0 112-113 0.0142004050852398 0.0 0.0 73.40164475569074 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGAC 15 2.681288E-4 119.21268 2 TATAGTT 20 8.4175996E-4 89.40951 2 TAGGGTC 30 4.257352E-5 79.346375 5 CTTTACA 55 1.9299478E-9 76.01441 1 ATAGTTT 25 0.0020413573 71.52761 3 GTATAGT 30 0.0041715465 59.72561 1 GTAGACC 30 0.004204705 59.60634 3 TAGGGAG 40 1.7691473E-4 59.509777 5 CTTTCGA 15 0.0042375103 59.494953 38-39 TGGACAG 45 3.1658687E-4 52.897583 5 TATTAGG 80 3.7847713E-8 52.15555 2 GTTTAAG 35 0.0076768366 51.193382 1 TAGACCA 35 0.007737754 51.09115 4 CTATAAT 35 0.007737754 51.09115 3 AGACCGC 35 0.00779519 50.995674 8 TACAGTG 35 0.00779519 50.995674 7 TGTCTTG 30 2.193891E-5 49.57913 16-17 GTATTAG 85 6.006303E-8 49.1858 1 GTCAGAT 125 1.0913936E-11 47.68507 3 GTACCAT 50 5.3306593E-4 47.59596 9 >>END_MODULE