##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935930_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 985845 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.98513255126313 31.0 31.0 34.0 28.0 34.0 2 31.026681679168632 33.0 31.0 34.0 27.0 34.0 3 31.22802063204662 34.0 31.0 34.0 27.0 34.0 4 34.41805861976274 37.0 35.0 37.0 31.0 37.0 5 34.56804467233693 37.0 35.0 37.0 31.0 37.0 6 34.650295939016786 37.0 35.0 37.0 32.0 37.0 7 34.57194893720615 37.0 35.0 37.0 31.0 37.0 8 34.545734877186575 37.0 35.0 37.0 31.0 37.0 9 36.12092164589768 39.0 37.0 39.0 31.0 39.0 10-11 35.916557369566206 39.0 37.0 39.0 30.5 39.0 12-13 35.75275930800481 39.0 37.0 39.0 30.0 39.0 14-15 37.179707763390795 40.0 38.0 41.0 30.5 41.0 16-17 37.338264128742345 40.0 38.0 41.0 31.0 41.0 18-19 37.408388235473126 40.0 38.0 41.0 31.0 41.0 20-21 37.53053218305109 40.0 38.0 41.0 31.0 41.0 22-23 37.44706622237776 40.0 38.0 41.0 31.0 41.0 24-25 37.42554154050586 40.0 38.0 41.0 31.0 41.0 26-27 37.3943231441048 40.0 37.0 41.0 30.5 41.0 28-29 37.293812921909634 40.0 37.0 41.0 30.0 41.0 30-31 37.2069118370535 40.0 37.0 41.0 30.0 41.0 32-33 37.118657091124874 40.0 37.0 41.0 30.0 41.0 34-35 36.90313994593471 40.0 36.0 41.0 30.0 41.0 36-37 36.8947248299682 40.0 36.0 41.0 30.0 41.0 38-39 37.00282803077563 40.0 36.0 41.0 30.0 41.0 40-41 36.97682039265808 40.0 36.0 41.0 30.0 41.0 42-43 36.83430610288636 40.0 35.5 41.0 30.0 41.0 44-45 36.67964893061283 40.0 35.0 41.0 30.0 41.0 46-47 36.52918257941157 40.0 35.0 41.0 30.0 41.0 48-49 36.370483189548054 40.0 35.0 41.0 30.0 41.0 50-51 36.1438430990673 39.0 35.0 41.0 30.0 41.0 52-53 35.898391735009056 39.0 35.0 41.0 29.5 41.0 54-55 35.63750437441991 39.0 35.0 41.0 29.0 41.0 56-57 35.348908296943236 38.0 35.0 40.5 29.0 41.0 58-59 34.962283117528614 37.0 34.5 40.0 28.0 41.0 60-61 34.637809189071305 37.0 34.0 40.0 28.0 41.0 62-63 34.232933169007296 36.0 34.0 39.0 27.0 41.0 64-65 33.816697351003455 36.0 34.0 39.0 26.5 41.0 66-67 33.50410257190532 35.0 33.5 38.0 26.5 40.0 68-69 33.305738731747894 35.0 34.0 37.0 27.0 40.0 70-71 33.11372223828289 35.0 34.0 37.0 27.0 39.0 72-73 32.845167343750795 35.0 34.0 36.0 26.5 39.0 74-75 32.54635160699705 35.0 33.0 36.0 26.0 38.0 76-77 32.35037100152661 35.0 33.0 35.0 26.5 37.0 78-79 32.15249506768305 35.0 33.0 35.0 26.0 37.0 80-81 31.951821533811096 35.0 33.0 35.0 26.0 36.0 82-83 31.82334545491431 35.0 33.0 35.0 26.0 36.0 84-85 31.61378309977735 35.0 33.0 35.0 25.0 36.0 86-87 31.437832012131725 35.0 33.0 35.0 25.0 35.0 88-89 31.33750843185288 35.0 33.0 35.0 24.5 35.0 90-91 31.28752947978638 35.0 33.0 35.0 24.0 35.0 92-93 31.18719575592512 35.0 33.0 35.0 23.5 36.0 94-95 31.035670414720368 35.0 33.0 35.0 23.0 35.0 96-97 30.926816588814674 35.0 32.5 35.0 21.5 35.0 98-99 30.7731058127799 35.0 32.0 35.0 20.0 35.0 100-101 30.66042836348513 35.0 32.0 35.0 19.0 35.0 102-103 29.982270032307312 34.0 30.5 35.0 18.0 35.0 104-105 30.486241244820434 35.0 32.0 35.0 18.5 35.0 106-107 30.514076756488087 35.0 32.0 35.0 18.0 35.0 108-109 30.41998336452485 35.0 32.0 35.0 16.0 35.0 110-111 30.31308420694937 35.0 32.0 35.0 14.0 35.0 112-113 30.233026997144584 35.0 32.0 35.0 8.5 35.0 114-115 30.084994598542366 35.0 31.0 35.0 2.0 35.0 116-117 29.916449847592673 35.0 31.0 35.0 2.0 35.0 118-119 29.69733020910995 35.0 31.0 35.0 2.0 35.0 120-121 29.477294097956573 35.0 31.0 35.0 2.0 35.0 122-123 29.078544801667604 35.0 31.0 35.0 2.0 35.0 124-125 26.377604998757413 32.0 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.060633948700868245 1101 2 0.26164368700082363 1101 3 0.3343135933016086 1101 4 0.2997941068722909 1101 5 0.27961138082272896 1101 6 0.2738118890466765 1101 7 0.27438834993397165 1101 8 0.1668002929562178 1101 9 0.08341844033520118 1101 10-11 0.42625674857735163 1101 12-13 0.2072364703352676 1101 14-15 0.18631731485027814 1101 16-17 0.11817544692854653 1101 18-19 0.2223651179216617 1101 20-21 0.20491458972557552 1101 22-23 0.19966111036350043 1101 24-25 0.21960078781761894 1101 26-27 0.28077414948666757 1101 28-29 0.22352049247781736 1101 30-31 0.1740746239973774 1101 32-33 0.2085791186995749 1101 34-35 -0.10539423038763118 1101 36-37 0.031884251910469175 1101 38-39 0.07088066017067973 1101 40-41 0.09936294869341822 1101 42-43 0.14795614887270858 1101 44-45 0.11960327220612044 1101 46-47 0.17257240760298487 1101 48-49 0.03192422958798602 1101 50-51 0.07870487820187577 1101 52-53 0.15956546754303247 1101 54-55 0.032136809826312174 1101 56-57 0.05216770967124518 1101 58-59 -0.08881315138341961 1101 60-61 -0.07094821993667466 1101 62-63 0.03495130546403402 1101 64-65 -0.01177030659653866 1101 66-67 -0.06780411889292282 1101 68-69 -0.033808840422217656 1101 70-71 -0.23768502570067085 1101 72-73 -0.0182372096336465 1101 74-75 0.07873144907956231 1101 76-77 0.1764467036103312 1101 78-79 0.1848461308010343 1101 80-81 0.06285729451434463 1101 82-83 0.07738649923447127 1101 84-85 0.02205287371525344 1101 86-87 -0.01441079558437508 1101 88-89 -0.015013248347564456 1101 90-91 0.0015238166496622796 1101 92-93 -0.0992231583979084 1101 94-95 -0.17943196066444145 1101 96-97 -0.07431541987438806 1101 98-99 -0.1671735718919578 1101 100-101 -0.09794346465596249 1101 102-103 -0.07941678921369544 1101 104-105 -0.20744620677803383 1101 106-107 0.14067212782572014 1101 108-109 0.06662693666271835 1101 110-111 -0.18514538480846454 1101 112-113 -0.1238615622253505 1101 114-115 -0.237987179940351 1101 116-117 -0.27242498535277093 1101 118-119 -0.27882398766147176 1101 120-121 -0.40191430516300386 1101 122-123 -0.3052258908138441 1101 124-125 -0.35988823654300006 1102 1 0.32933574519336517 1102 2 0.36299633050514757 1102 3 0.440133768704154 1102 4 0.5061422433914728 1102 5 0.4094925553238795 1102 6 0.37363791120076684 1102 7 0.4961665405095772 1102 8 0.332060067908337 1102 9 0.34843793407336676 1102 10-11 0.4366406231598745 1102 12-13 0.574954949522315 1102 14-15 0.6704376582030989 1102 16-17 0.6765687135749374 1102 18-19 0.6504402105956899 1102 20-21 0.6048047234008322 1102 22-23 0.5788265098686836 1102 24-25 0.5264412679416708 1102 26-27 0.6104161386972962 1102 28-29 0.5324525424848048 1102 30-31 0.47516369791468094 1102 32-33 0.511410436903688 1102 34-35 0.5668553442570854 1102 36-37 0.49544424960902944 1102 38-39 0.48721731689185077 1102 40-41 0.5372271035035681 1102 42-43 0.46236452830332553 1102 44-45 0.49383372846645557 1102 46-47 0.4051616089213752 1102 48-49 0.40891696087577856 1102 50-51 0.37894926912872506 1102 52-53 0.5277639611962712 1102 54-55 0.472198291131221 1102 56-57 0.3876624780067246 1102 58-59 0.4498173278913029 1102 60-61 0.37064618427825025 1102 62-63 0.408238445624427 1102 64-65 0.35132063587021634 1102 66-67 0.29441282463140084 1102 68-69 0.46782408550158294 1102 70-71 0.5075497621970513 1102 72-73 0.37581850163436314 1102 74-75 0.4284915788368906 1102 76-77 0.5176361641146272 1102 78-79 0.3898429937201868 1102 80-81 0.3972901130376414 1102 82-83 0.37430311863944254 1102 84-85 0.36971485804902926 1102 86-87 0.4142750004259135 1102 88-89 0.3644143508403559 1102 90-91 0.4398682599068593 1102 92-93 0.45760211578843624 1102 94-95 0.37269289789293936 1102 96-97 0.29748756596902126 1102 98-99 0.2997851583711437 1102 100-101 0.3643553991454134 1102 102-103 0.43246658261395154 1102 104-105 0.2794270962530163 1102 106-107 0.1529582515171235 1102 108-109 0.23122723968554837 1102 110-111 0.19876234939722792 1102 112-113 0.08477732826176165 1102 114-115 5.021991916258628E-4 1102 116-117 0.13753723268667883 1102 118-119 0.18171838484838432 1102 120-121 -0.09956217948250412 1102 122-123 -0.15516027239794283 1102 124-125 0.013792888093632882 1103 1 0.4045923710964203 1103 2 0.32772960078590785 1103 3 0.3357057870149589 1103 4 0.33255878634241043 1103 5 0.541226572887922 1103 6 0.5678895539899003 1103 7 0.488716814084583 1103 8 0.49223968897322123 1103 9 0.41293063480137704 1103 10-11 0.5717003926941402 1103 12-13 0.4299331533221604 1103 14-15 0.5280699517641168 1103 16-17 0.43876878278922504 1103 18-19 0.43259846765588605 1103 20-21 0.4283686712622057 1103 22-23 0.49377695443134684 1103 24-25 0.6219283700842198 1103 26-27 0.5047734934510046 1103 28-29 0.4844229179277022 1103 30-31 0.5428414113853677 1103 32-33 0.5406700172802346 1103 34-35 0.5607260983830287 1103 36-37 0.5556822144357767 1103 38-39 0.5539358505151384 1103 40-41 0.53350906719389 1103 42-43 0.5217039676885662 1103 44-45 0.46246122166449766 1103 46-47 0.5159425488388578 1103 48-49 0.41545450805362094 1103 50-51 0.4953455991958222 1103 52-53 0.5588158661384739 1103 54-55 0.51550711238243 1103 56-57 0.4368374467999061 1103 58-59 0.36308599119947615 1103 60-61 0.42356124552869545 1103 62-63 0.16725218923011198 1103 64-65 0.2557734044681439 1103 66-67 0.35616242328828207 1103 68-69 0.3906677425999092 1103 70-71 0.5059367751172275 1103 72-73 0.36760048606797113 1103 74-75 0.35738098353621695 1103 76-77 0.20452341676411834 1103 78-79 0.23701279612336634 1103 80-81 0.22516078629104186 1103 82-83 0.30195422819495477 1103 84-85 0.31200100925652663 1103 86-87 0.35396640341221186 1103 88-89 0.23831357840255407 1103 90-91 0.24263063484981373 1103 92-93 0.183248321527131 1103 94-95 0.2629310045092055 1103 96-97 0.03351775348303576 1103 98-99 0.1869921201315634 1103 100-101 0.18691447942506656 1103 102-103 0.24755703119923567 1103 104-105 0.174019979154302 1103 106-107 0.12930093263091536 1103 108-109 0.22320221541065877 1103 110-111 0.1719864487843239 1103 112-113 0.1900100967651639 1103 114-115 0.1304817027287477 1103 116-117 0.2708553542801191 1103 118-119 0.25652154685971595 1103 120-121 0.17284095858214954 1103 122-123 -0.02881924549522097 1103 124-125 -0.12014753194993943 1104 1 0.21178456004963309 1104 2 0.2855093510502478 1104 3 0.33221189248954985 1104 4 0.2960709283331511 1104 5 0.36936091101063084 1104 6 0.3546912613302666 1104 7 0.18516174104167504 1104 8 0.15290756034981 1104 9 0.24123471678766606 1104 10-11 0.31948222939346493 1104 12-13 0.12159519328977098 1104 14-15 0.3101771803772735 1104 16-17 0.36825965728839805 1104 18-19 0.23059796549696188 1104 20-21 0.29844212084650223 1104 22-23 0.1938430838115366 1104 24-25 0.2425331533248425 1104 26-27 0.3194874573592088 1104 28-29 0.33714359379118264 1104 30-31 0.27291860947998714 1104 32-33 0.2958516255326131 1104 34-35 0.45561044269430795 1104 36-37 0.30506424068241955 1104 38-39 0.256981338334235 1104 40-41 0.3972206582243416 1104 42-43 0.3572822814954577 1104 44-45 0.21212792251569113 1104 46-47 0.26596288273918134 1104 48-49 0.40301016105473053 1104 50-51 0.38470265894920175 1104 52-53 0.2744966628030099 1104 54-55 0.31306810524257855 1104 56-57 0.2655393659664611 1104 58-59 0.3168095703902978 1104 60-61 0.21326451203717767 1104 62-63 0.27965975839189383 1104 64-65 0.1651231086541003 1104 66-67 0.301015117441672 1104 68-69 0.2313206906618177 1104 70-71 0.3254371623454162 1104 72-73 0.24605680708191358 1104 74-75 0.2665503348671123 1104 76-77 0.3173447365933839 1104 78-79 0.2716652986076369 1104 80-81 0.20918010880085092 1104 82-83 0.3148317756790284 1104 84-85 0.29914291159014894 1104 86-87 0.29868648517217267 1104 88-89 0.26465924056275725 1104 90-91 0.13900731062842553 1104 92-93 0.05178169918247377 1104 94-95 0.1020138218329798 1104 96-97 0.10896006001923553 1104 98-99 0.006397026436488318 1104 100-101 0.07906170393608036 1104 102-103 0.0696866801477043 1104 104-105 0.10896387527105844 1104 106-107 0.31355612838449076 1104 108-109 0.2912429822285638 1104 110-111 0.13601843135729297 1104 112-113 0.22903704006717618 1104 114-115 0.2769954113138944 1104 116-117 0.07651454396535229 1104 118-119 -0.09828910032412708 1104 120-121 0.016653617938043652 1104 122-123 0.026824914785422038 1104 124-125 0.07395457605666422 1105 1 0.08815943042809593 1105 2 0.06806028324715996 1105 3 0.09193620292258942 1105 4 0.2757661682579524 1105 5 0.09799369540860425 1105 6 0.1795326280950107 1105 7 -0.07229407002158439 1105 8 -0.08531231697774189 1105 9 -0.053323237024926584 1105 10-11 0.11340138672117916 1105 12-13 0.09415732831571688 1105 14-15 0.1374411711719432 1105 16-17 0.19131009060166804 1105 18-19 0.10969415300663599 1105 20-21 0.12846810396091257 1105 22-23 0.26621380042014664 1105 24-25 0.26067722778297764 1105 26-27 0.23814663652411383 1105 28-29 0.20100398282241372 1105 30-31 0.1108230825745764 1105 32-33 0.3475831144013313 1105 34-35 0.348122091480235 1105 36-37 0.2646453548493426 1105 38-39 0.20867342617696494 1105 40-41 0.28226228413414844 1105 42-43 0.3821879934680794 1105 44-45 0.3842898234877765 1105 46-47 0.2987343430666627 1105 48-49 0.2794271768092571 1105 50-51 0.25909620659014365 1105 52-53 0.29993066216397324 1105 54-55 0.34145889677193964 1105 56-57 0.38830664116007796 1105 58-59 0.4142790836014001 1105 60-61 0.2922375958437442 1105 62-63 0.2525073548312946 1105 64-65 0.06613440165463658 1105 66-67 0.23486402920885752 1105 68-69 0.33469423199075266 1105 70-71 0.2617672606127499 1105 72-73 0.3548497047039376 1105 74-75 0.38277331527049796 1105 76-77 0.5037231060181924 1105 78-79 0.47060740066217477 1105 80-81 0.41578286804882225 1105 82-83 0.39251722023433544 1105 84-85 0.47366862909067464 1105 86-87 0.4524102741786997 1105 88-89 0.410712081063096 1105 90-91 0.43853204800392476 1105 92-93 0.43978918341716167 1105 94-95 0.3257413255133521 1105 96-97 0.2905375320415686 1105 98-99 0.3355595194040859 1105 100-101 0.5389432264230827 1105 102-103 0.4976462656724365 1105 104-105 0.43231128646683104 1105 106-107 0.5302919102008197 1105 108-109 0.43915413977581963 1105 110-111 0.5235395285484472 1105 112-113 0.5738805533057239 1105 114-115 0.5970910127088089 1105 116-117 0.6112526251549895 1105 118-119 0.48580440164298366 1105 120-121 0.6050680010767593 1105 122-123 0.5377104283415335 1105 124-125 0.5633040474752384 1106 1 -0.05890203228246449 1106 2 0.05157008982662603 1106 3 0.12940797070339727 1106 4 -0.47655827650044813 1106 5 0.1546948739546039 1106 6 0.25439168178347416 1106 7 0.14632926683103165 1106 8 0.15353893955886377 1106 9 0.18492041824321603 1106 10-11 0.42094809994674165 1106 12-13 0.2679635087613619 1106 14-15 0.2989430679570404 1106 16-17 0.41238650421728096 1106 18-19 0.315497246284167 1106 20-21 0.31360583327908387 1106 22-23 0.37785611848818945 1106 24-25 0.4238484768355377 1106 26-27 0.3492061728570448 1106 28-29 0.2909993721167865 1106 30-31 0.21556294522709862 1106 32-33 0.253008759852392 1106 34-35 0.2175512994889246 1106 36-37 0.17348654992896684 1106 38-39 0.3285570225655263 1106 40-41 0.34314030376448557 1106 42-43 0.21287080746859033 1106 44-45 0.17073110333565467 1106 46-47 0.18918760502155862 1106 48-49 0.08785474481955191 1106 50-51 0.06996229375252483 1106 52-53 0.19427653381643495 1106 54-55 0.2545910073296014 1106 56-57 0.3230520309317626 1106 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2216 124-125 -0.8032680237679628 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14686.0 3 2980.0 4 2148.0 5 1545.0 6 1786.0 7 1896.0 8 2177.0 9 2631.0 10 3514.0 11 4258.0 12 4086.0 13 3551.0 14 3470.0 15 3394.0 16 3328.0 17 3116.0 18 3040.0 19 2778.0 20 2871.0 21 2890.0 22 3500.0 23 4836.0 24 7118.0 25 10951.0 26 13284.0 27 11085.0 28 12807.0 29 16418.0 30 18180.0 31 22359.0 32 29796.0 33 40304.0 34 65654.0 35 94553.0 36 193791.0 37 313184.0 38 57722.0 39 158.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 66.60469180204619 11.008971115649162 7.146413077712416 15.239924004592236 2 12.009447342630525 30.768895192528156 42.03418168684575 15.187475777995566 3 35.83109735994522 20.12771677106845 19.991580228754028 24.049605640232304 4 14.235640842248362 15.326546244020717 46.45321321504116 23.984599698689756 5 16.549556156746508 33.52758766550926 28.55558970644772 21.367266471296514 6 31.206325537990253 38.17212644989831 15.155120733989625 15.466427278121813 7 42.42430082680508 24.138934901399107 16.966190356882386 16.47057391491343 8 39.86433917842736 25.468812922164936 16.289746520990708 18.377101378417006 9 51.77882932915418 11.550700160775781 11.712490300199322 24.95798020987072 10-11 38.41445663364931 24.9110661412291 20.15864562887675 16.515831596244844 12-13 29.018608401929306 28.025754555736448 24.29002530823811 18.665611734096128 14-15 25.021986214871504 28.52578245058807 22.402862518955818 24.0493688155846 16-17 23.630946041213374 32.60634278208035 21.661670952330233 22.101040224376042 18-19 23.923892701185277 26.474445780016126 25.03076041365529 24.570901105143303 20-21 24.318376621071263 27.71277432050677 22.627542869315157 25.341306189106806 22-23 24.924049926712616 24.66143257814362 22.565869888268438 27.84864760687532 24-25 22.477722157134234 24.101151803782543 31.450278694926688 21.970847344156535 26-27 19.779557095594743 23.453808376363757 30.164120006065932 26.602514521975568 28-29 22.665301905272507 24.442754836474634 23.67624315949702 29.215700098755836 30-31 23.120875999776842 27.569597654803747 22.477620721310146 26.831905624109265 32-33 29.040670693668886 24.96305200107522 20.77223092879712 25.224046376458777 34-35 28.060851350871584 20.182381611713808 26.046589474004534 25.71017756341007 36-37 24.848987416886022 25.49848099853425 28.019009073434464 21.633522511145262 38-39 23.17894800906836 28.78282082883212 24.82159974438172 23.216631417717796 40-41 21.350617997758267 28.44412661219563 26.4879367446201 23.717318645426005 42-43 21.965978424600216 22.850650964401098 24.55279481054324 30.630575800455446 44-45 23.392166111305528 24.184836358656785 23.608528724089485 28.814468805948195 46-47 24.655802889906628 22.87879940558607 24.27480993462461 28.19058776988269 48-49 19.15534389280262 23.497659368359123 25.945305803650676 31.40169093518758 50-51 23.046269951158653 25.18235625275779 25.108663126556408 26.662710669527158 52-53 24.28748941263586 22.890616679092552 27.995983141366036 24.825910766905547 54-55 21.643615375642216 24.727416581714166 27.81669532228697 25.81227272035665 56-57 21.449278890532153 26.314206536138652 25.71730561286697 26.519208960462226 58-59 23.561766809183997 23.580735308288826 29.254852436234906 23.602645446292268 60-61 25.22074971217585 28.007242517840027 27.019967641972116 19.75204012801201 62-63 26.201583413214045 25.821401944524748 25.24225410688293 22.73476053537828 64-65 29.779626201404913 21.236629219181903 26.943879491796213 22.03986508761697 66-67 29.645446112444713 24.630253554186286 23.63146520571956 22.09283512764944 68-69 23.932674769878126 28.328575689148312 25.044634614955434 22.694114926018123 70-71 24.783303666044915 27.847463637069215 23.232048585025062 24.13718411186081 72-73 23.278721948542593 28.03875256188983 23.55564188875629 25.126883600811283 74-75 21.573330749850747 28.51231474121975 25.519008632517693 24.395345876411806 76-77 22.832937224029482 28.27464728013398 23.221388076552056 25.671027419284485 78-79 23.61345543662612 29.71038675961016 20.40530232331675 26.270855480446976 80-81 22.98491580981981 24.066285026832624 24.458896963838214 28.48990219950935 82-83 24.900732316303433 20.568000328652236 27.408024287806036 27.12324306723829 84-85 26.047717074031873 21.757421297569202 30.343795877412006 21.85106575098692 86-87 27.884051620075915 24.04728864760027 24.769683653575 23.298976078748815 88-89 23.800665752888193 26.23661924961269 26.644420793972714 23.3182942035264 90-91 20.835334578627766 28.17117709740228 26.27082668717024 24.722661636799717 92-93 24.114690067972 23.680379425788782 26.91696256467485 25.28796794156437 94-95 23.285991751111005 23.471289940180085 27.645495600372726 25.597222708336187 96-97 23.006240768693903 22.56897104413173 27.66962271347649 26.75516547369788 98-99 21.983192578319755 24.83819217509988 28.771437222722934 24.40717802385743 100-101 20.977685988975878 28.89876430001684 25.80510185243417 24.318447858573112 102-103 22.346058457465425 27.44599810315009 25.419969670688598 24.78797376869589 104-105 22.797473625270644 24.31237539173466 25.153790246789665 27.73636073620503 106-107 21.921072959000725 26.032057104919744 23.24051349389781 28.806356442181723 108-109 23.9186180383326 26.17734025125654 25.058604547367995 24.84543716304287 110-111 25.166836571672018 27.3450187402685 23.049769487089755 24.438375200969727 112-113 22.462543155063045 28.358613945849935 24.28673372660421 24.892109172482805 114-115 23.69242598922382 25.28181840775706 25.5710293973689 25.454726205650214 116-117 26.548848956986138 24.370971095861925 24.172613341853943 24.907566605297994 118-119 23.52064285957191 25.849140974227453 25.606779423580484 25.023436742620152 120-121 20.942640993441035 26.474296681158744 27.02911061384058 25.553951711559648 122-123 22.10735124651042 27.166633529182626 28.128956209829255 22.5970590144777 124-125 23.247681074014963 28.55750422905594 23.823758065967677 24.37105663096142 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 2.0 2 5.0 3 6.0 4 15.5 5 26.0 6 33.5 7 36.0 8 44.0 9 58.0 10 67.0 11 82.5 12 98.0 13 118.0 14 153.5 15 206.5 16 241.5 17 301.0 18 403.5 19 478.5 20 578.5 21 690.0 22 825.5 23 1025.5 24 1258.0 25 1493.5 26 1841.5 27 2272.5 28 2701.0 29 3156.0 30 3731.5 31 4307.0 32 4977.0 33 5791.5 34 6884.5 35 7805.5 36 8548.5 37 9702.0 38 10549.0 39 11127.5 40 11718.5 41 12829.5 42 13886.5 43 15029.5 44 16971.5 45 20745.0 46 34203.5 47 59195.0 48 79119.0 49 92676.5 50 93084.5 51 74360.5 52 55697.0 53 44500.5 54 38124.5 55 33475.0 56 29350.5 57 25745.5 58 23098.0 59 20180.0 60 17800.0 61 15693.0 62 12329.0 63 9185.5 64 7463.5 65 6734.0 66 5965.0 67 5070.0 68 4214.0 69 3285.0 70 2484.0 71 1968.5 72 1777.5 73 1518.0 74 1109.0 75 855.0 76 641.5 77 453.5 78 379.0 79 329.5 80 284.0 81 256.5 82 184.0 83 88.0 84 33.5 85 22.5 86 16.0 87 14.5 88 9.5 89 5.5 90 9.5 91 10.5 92 7.5 93 5.5 94 2.5 95 2.0 96 1.5 97 1.5 98 0.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15976142294174034 2 0.01734552591938895 3 0.007100507686299571 4 0.01521537361349908 5 0.0024344597781598525 6 0.0 7 2.0287164817998771E-4 8 4.0574329635997543E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0011665119770349294 28-29 0.008165583839244506 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.0430747226998156E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.002028716481799877 66-67 5.071791204499693E-5 68-69 0.006390456917669613 70-71 0.019424960313233826 72-73 5.071791204499693E-5 74-75 0.009687121200594413 76-77 6.086149445399631E-4 78-79 0.0013186657131699204 80-81 0.0011665119770349294 82-83 5.071791204499693E-5 84-85 0.020490036466178762 86-87 0.0027387672504298344 88-89 0.01967854987345881 90-91 5.071791204499693E-5 92-93 0.014708194493049111 94-95 0.013845989988284162 96-97 0.006999071862209576 98-99 0.003093792634744813 100-101 7.10050768629957E-4 102-103 0.0 104-105 5.578970324949663E-4 106-107 0.0010650761529449357 108-109 0.0 110-111 0.0 112-113 0.0010650761529449357 114-115 0.006035431533354635 116-117 0.0 118-119 0.07572184268318043 120-121 0.0019272806577098833 122-123 0.005376098676769675 124-125 0.25049576759023984 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 985845.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.73163595996875 #Duplication Level Percentage of deduplicated Percentage of total 1 82.71861397843128 39.482947695316646 2 9.496008206694679 9.065200135896522 3 2.7009775050104 3.8676622501566325 4 1.2626711987352632 2.410774479806758 5 0.7833180194882109 1.8694525273547493 6 0.49132907378712987 1.4071164291933547 7 0.3607789579485012 1.2054398917970313 8 0.26835325309743335 1.0247151828416037 9 0.20193382574737884 0.8674768672719885 >10 1.4616723045835394 13.333451634223373 >50 0.14099617625714966 4.690053788257728 >100 0.09909931539855904 9.1106572485134 >500 0.007655741097589272 2.4943031638186146 >1k 0.005316486873325884 4.407282345250495 >5k 0.001275956849598212 4.763466360301141 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 9774 0.9914337446556001 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 9408 0.9543082330386622 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 8390 0.8510465641150485 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 7589 0.7697964690189635 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 6364 0.645537584508721 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 5403 0.5480577575582368 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 4462 0.4526066470895526 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3155 0.32003002500393063 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 2877 0.2918308659069124 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 2415 0.24496751517733517 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2382 0.24162013298236537 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2249 0.22812916837839622 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 1869 0.18958355522419854 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1811 0.1837002774269789 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1791 0.181671560945179 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1726 0.1750782323793294 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1719 0.17436818161069945 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1680 0.1704121844711897 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1602 0.16250019019217016 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1397 0.14170584625372143 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1383 0.14028574471646152 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1222 0.1239545770379725 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1178 0.11949140077801278 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1149 0.11654976187940294 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1090 0.1105650482580933 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1075 0.10904351089674341 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1050 0.10650761529449357 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1050 0.10650761529449357 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1046 0.10610187199813358 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1036 0.10508751375723364 No Hit ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA 1005 0.10194300321044382 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.0143582408999386E-4 0.0 14-15 0.0 0.0 0.0 1.0143582408999386E-4 0.0 16-17 0.0 0.0 0.0 1.5215373613499078E-4 0.0 18-19 0.0 0.0 0.0 2.0287164817998771E-4 0.0 20-21 0.0 0.0 0.0 8.11486592719951E-4 0.0 22-23 0.0 0.0 0.0 0.0026373314263398407 0.0 24-25 1.0143582408999386E-4 0.0 0.0 0.003955997139509761 0.0 26-27 1.0143582408999386E-4 0.0 0.0 0.005933995709264641 0.0 28-29 1.0143582408999386E-4 0.0 0.0 0.012578042187159239 0.0 30-31 1.0143582408999386E-4 0.0 0.0 0.034336026454462924 0.0 32-33 1.0143582408999386E-4 0.0 0.0 0.07957640399860019 0.0 34-35 1.0143582408999386E-4 0.0 0.0 0.1338952877987919 0.0 36-37 1.0143582408999386E-4 0.0 0.0 0.20419031389315764 0.0 38-39 1.0143582408999386E-4 0.0 0.0 0.31805202643417574 0.0 40-41 1.0143582408999386E-4 0.0 0.0 0.4877541601367355 0.0 42-43 1.0143582408999386E-4 0.0 0.0 0.8290349902875198 0.0 44-45 1.0143582408999386E-4 0.0 0.0 1.3018273663709814 0.0 46-47 2.0287164817998771E-4 0.0 0.0 1.7902915772763466 0.0 48-49 2.0287164817998771E-4 0.0 0.0 1.9949383523779094 0.0 50-51 2.0287164817998771E-4 0.0 0.0 2.2581643158914435 0.0 52-53 2.0287164817998771E-4 0.0 0.0 2.4833518453712298 0.0 54-55 2.0287164817998771E-4 0.0 0.0 2.713459012319381 0.0 56-57 3.043074722699816E-4 0.0 0.0 3.039321597208486 0.0 58-59 3.043074722699816E-4 0.0 0.0 3.4137212239246537 0.0 60-61 3.043074722699816E-4 0.0 0.0 3.9255663922827626 0.0 62-63 3.043074722699816E-4 0.0 0.0 4.535347848799761 0.0 64-65 3.043074722699816E-4 0.0 0.0 5.087970218442047 0.0 66-67 3.043074722699816E-4 0.0 0.0 5.558125263099169 0.0 68-69 3.043074722699816E-4 0.0 0.0 5.979540394281049 0.0 70-71 3.043074722699816E-4 0.0 0.0 6.44462364773367 0.0 72-73 3.043074722699816E-4 0.0 0.0 6.890484812521239 0.0 74-75 3.043074722699816E-4 0.0 0.0 7.328839726326146 0.0 76-77 3.043074722699816E-4 0.0 0.0 7.8369824871049705 0.0 78-79 3.043074722699816E-4 0.0 0.0 8.486983247873653 0.0 80-81 3.043074722699816E-4 0.0 0.0 10.116448326055313 0.0 82-83 3.043074722699816E-4 0.0 0.0 11.294879012420816 0.0 84-85 3.043074722699816E-4 0.0 0.0 11.853993274804862 0.0 86-87 3.043074722699816E-4 0.0 0.0 12.329879443523069 0.0 88-89 3.043074722699816E-4 0.0 0.0 12.88295827437376 0.0 90-91 3.550253843149785E-4 0.0 0.0 13.545892102713914 0.0 92-93 4.0574329635997543E-4 0.0 0.0 14.337700145560408 0.0 94-95 4.0574329635997543E-4 0.0 0.0 15.2663451151043 0.0 96-97 4.0574329635997543E-4 0.0 0.0 16.284912942703976 0.0 98-99 4.0574329635997543E-4 0.0 0.0 17.3440043820276 0.0 100-101 4.0574329635997543E-4 0.0 0.0 18.3587683662239 0.0 102-103 4.0574329635997543E-4 0.0 0.0 19.26058356029599 0.0 104-105 6.593328565849602E-4 0.0 0.0 20.19891565104048 0.0 106-107 7.10050768629957E-4 0.0 0.0 21.101593049617335 0.0 108-109 7.10050768629957E-4 0.0 0.0 21.88320679214278 0.0 110-111 8.114865927199509E-4 0.0 0.0 22.76640851249436 0.0 112-113 8.114865927199509E-4 0.0 0.0 25.313563491218193 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGT 1730 0.0 91.94382 1 AGTAGTA 1360 0.0 91.87739 2 GAGTCCG 535 0.0 86.85567 1 GAGTTAG 830 0.0 86.131195 1 GAGTCAG 4300 0.0 82.711 1 GAGTCGA 385 0.0 77.368935 1 GAGTCGT 580 0.0 76.00831 1 GAGTAAG 830 0.0 75.3648 1 AGTCGAC 120 0.0 74.37693 2 GAGTAGG 965 0.0 73.46441 1 GAGTACG 490 0.0 72.94785 1 AGTCCGT 290 0.0 71.81221 2 GAGTTAC 520 0.0 71.03063 1 AGTCGTA 420 0.0 70.835175 2 GTGGGGT 4995 0.0 70.62873 7 TGGGGTA 5215 0.0 70.4976 8 AGTCGAG 195 0.0 70.18132 2 AGTCAGT 3460 0.0 69.99171 2 GAGTCGC 400 0.0 67.020836 1 AGTTAGT 590 0.0 66.56106 2 >>END_MODULE