FastQCFastQC Report
Thu 26 May 2016
SRR935929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309271
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC179391.370151786757669No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT168891.28995448612243No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT152781.166908913433506No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG144011.0999250728076921No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT127840.9764212298294241No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA103570.7910508977896861No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT81360.6214145123507662No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC59590.45513877570037065No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC52100.3979313679139002No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG47670.36409574488398505No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG46200.3528681227950516No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG46130.35233347412415No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG44150.33721055457579063No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA39550.3020764990594002No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC35930.2744275249356321No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT35460.2708377410024357No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA35090.268011740884813No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA33060.2525069294286668No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC32500.24822974006145404No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA32030.24463995612825765No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC31950.24402892907579868No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT27520.21019330604588357No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG26450.2020208192192449No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG24240.18514119689506603No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT23960.18300260221145967No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT23850.1821624400143286No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC23630.1804821156200664No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT23610.1803293588569517No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG22340.17062930439916565No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT22060.16849070971555927No Hit
GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA21660.16543557445326446No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA20800.15886703363933058No Hit
GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA20300.15504811456146206No Hit
GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA19540.1492433575631019No Hit
CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA18070.13801573547416845No Hit
ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA17650.13480784344875887No Hit
GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG17030.13007238379220193No Hit
GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG16950.12946135673974296No Hit
GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG16800.1283156810163824No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA16700.1275518972008087No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA15840.12098335638687484No Hit
GAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGA15800.12067784286064534No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG15680.1197613022819569No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC15470.11815735626925213No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG15030.11479670748072782No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG14260.10891557210081029No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA14100.10769351799589236No Hit
GAGTAAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC13460.10280530157622066No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCACGT13320.10173600423441748No Hit
GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG13250.10120135556351588No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13200.10081946365572902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT32550.095.351461
AGTAGTA27650.094.671062
GAGTTAG15800.093.505081
GAGTCCG9050.092.155251
GAGTACG8200.090.810651
AGTCCGT3950.088.8616942
AGTCGTA5950.087.988392
GAGTAGG13800.087.199281
GAGTCAG71050.086.444081
GAGTAAG12350.086.342911
AGTCGGT3950.085.8494342
GAGTCGT10850.085.1025541
GAGTTCG5150.084.441561
GAGTCGG9900.084.242931
AGTTCGT1000.083.289012
AGTTGTA5550.082.538662
GAGTTGC4700.082.386511
AGTTAGT11350.082.293112
AGTCCGG3700.082.002692
GAGTTAC8200.079.9133761
Produced by FastQC (version 0.11.5)