##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935929_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1309271 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.463880281469613 33.0 31.0 34.0 30.0 34.0 2 31.498071827757585 33.0 31.0 34.0 29.0 34.0 3 31.64370096030539 34.0 31.0 34.0 30.0 34.0 4 34.861293803956556 37.0 35.0 37.0 32.0 37.0 5 35.015559803890866 37.0 35.0 37.0 33.0 37.0 6 35.1322499314504 37.0 35.0 37.0 33.0 37.0 7 35.14881029213967 37.0 35.0 37.0 33.0 37.0 8 35.123855947317246 37.0 35.0 37.0 33.0 37.0 9 36.80126345118772 39.0 38.0 39.0 34.0 39.0 10-11 36.50076225624794 39.0 37.0 39.0 32.0 39.0 12-13 36.306584733030824 39.0 37.0 39.0 32.0 39.0 14-15 37.813697851705264 40.0 38.0 41.0 33.0 41.0 16-17 37.924118841706566 40.5 38.0 41.0 33.0 41.0 18-19 37.927445502115305 41.0 38.0 41.0 33.0 41.0 20-21 38.03885368269823 41.0 38.0 41.0 33.0 41.0 22-23 37.897009098956595 40.5 38.0 41.0 32.5 41.0 24-25 37.81222604029266 40.0 38.0 41.0 32.0 41.0 26-27 37.78181751524322 40.0 38.0 41.0 32.0 41.0 28-29 37.65586154432505 40.0 38.0 41.0 32.0 41.0 30-31 37.56338527317874 40.0 37.5 41.0 32.0 41.0 32-33 37.462341638973136 40.0 37.0 41.0 31.5 41.0 34-35 37.18740619780015 40.0 37.0 41.0 31.0 41.0 36-37 37.18331613546775 40.0 37.0 41.0 31.0 41.0 38-39 37.28228189580308 40.0 37.0 41.0 31.0 41.0 40-41 37.23357158296487 40.0 36.0 41.0 31.0 41.0 42-43 37.035634715807504 40.0 35.5 41.0 31.0 41.0 44-45 36.80053251007622 40.0 35.0 41.0 30.5 41.0 46-47 36.605916193057055 39.5 35.0 41.0 30.0 41.0 48-49 36.418595920936156 39.0 35.0 41.0 30.0 41.0 50-51 36.143493211107554 39.0 35.0 41.0 30.0 41.0 52-53 35.84536394680704 38.5 35.0 41.0 30.0 41.0 54-55 35.54365979235773 38.0 35.0 40.0 29.5 41.0 56-57 35.203250511162324 37.0 35.0 40.0 29.0 41.0 58-59 34.77024351719392 36.5 35.0 39.0 28.5 41.0 60-61 34.431980850412174 36.0 34.0 39.0 28.0 41.0 62-63 33.94785075053217 35.5 34.0 38.5 27.5 40.0 64-65 33.44003074993641 35.0 34.0 37.0 26.5 39.5 66-67 33.111491433018834 35.0 33.5 36.5 26.5 39.0 68-69 32.936713636825374 35.0 34.0 36.0 27.5 39.0 70-71 32.83963022170353 35.0 34.0 36.0 28.0 37.0 72-73 32.63353117880103 35.0 34.0 35.0 27.5 37.0 74-75 32.374221990710865 35.0 34.0 35.0 27.0 36.5 76-77 32.26930291742504 35.0 34.0 35.0 27.0 36.0 78-79 32.14724033450676 35.0 33.5 35.0 27.0 35.5 80-81 32.02957103609566 35.0 33.0 35.0 27.0 35.0 82-83 31.993303907288865 35.0 33.0 35.0 27.0 35.0 84-85 31.77862107997504 35.0 33.0 35.0 25.5 35.0 86-87 31.627937226135764 35.0 33.0 35.0 25.0 35.0 88-89 31.51641371419668 35.0 33.0 35.0 25.0 35.0 90-91 31.461301365416325 35.0 33.0 35.0 24.5 35.0 92-93 31.399262643104443 35.0 33.0 35.0 24.0 35.0 94-95 31.24045136568365 35.0 33.0 35.0 23.5 35.0 96-97 31.130187333256444 35.0 33.0 35.0 23.0 35.0 98-99 30.968577933827298 35.0 33.0 35.0 21.5 35.0 100-101 30.814910740404393 35.0 32.0 35.0 20.0 35.0 102-103 30.095315637480702 34.0 30.5 35.0 19.0 35.0 104-105 30.6134711606688 35.0 32.0 35.0 19.0 35.0 106-107 30.62435928085171 35.0 32.0 35.0 18.5 35.0 108-109 30.498963545362265 35.0 32.0 35.0 17.5 35.0 110-111 30.393262739341207 35.0 32.0 35.0 17.0 35.0 112-113 30.310451388597166 35.0 32.0 35.0 12.5 35.0 114-115 30.095721970470592 35.0 32.0 35.0 4.5 35.0 116-117 29.859852925788473 35.0 31.0 35.0 2.0 35.0 118-119 29.552190111901965 35.0 31.0 35.0 2.0 35.0 120-121 29.203450622522 35.0 31.0 35.0 2.0 35.0 122-123 28.63640720675857 35.0 30.0 35.0 2.0 35.0 124-125 25.63579312457085 32.0 23.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11891993559812164 1101 2 0.3330971290413771 1101 3 0.24660314218723656 1101 4 0.17490261457523104 1101 5 0.20652825929058594 1101 6 0.16484685196051885 1101 7 0.16014399411451308 1101 8 0.15711519522913875 1101 9 0.20559666312340852 1101 10-11 0.308442805342672 1101 12-13 0.13771181965967116 1101 14-15 0.303103865988426 1101 16-17 0.309107543014278 1101 18-19 0.35324891206386866 1101 20-21 0.16844555668534156 1101 22-23 0.22499975908540648 1101 24-25 0.25148648691941133 1101 26-27 0.26805847977811226 1101 28-29 0.25497825553765097 1101 30-31 0.03629048258486023 1101 32-33 0.16538996052968002 1101 34-35 -0.15250364179880194 1101 36-37 -0.1338294927536765 1101 38-39 0.0302756952982719 1101 40-41 -0.01976169302346875 1101 42-43 -0.11774232235376303 1101 44-45 -0.022400754779802412 1101 46-47 0.06912043450794414 1101 48-49 0.10020341385652642 1101 50-51 0.2293199782660693 1101 52-53 0.12661022774284447 1101 54-55 0.0866452776220541 1101 56-57 0.061906979191540756 1101 58-59 -0.11821239823204621 1101 60-61 0.028591142524348356 1101 62-63 -0.016324220122875488 1101 64-65 0.017952915384285006 1101 66-67 -0.30519152555081064 1101 68-69 -0.12822991731592737 1101 70-71 -0.18673286151054214 1101 72-73 -0.09358336813524915 1101 74-75 -0.061756679639266565 1101 76-77 -0.0660510819964486 1101 78-79 -0.07132785593010738 1101 80-81 0.06813593430522502 1101 82-83 -3.764574630089612E-4 1101 84-85 -0.13350898215390217 1101 86-87 -0.1450227799177739 1101 88-89 -0.25941376056347565 1101 90-91 -0.3308771680584073 1101 92-93 -0.5678950421044426 1101 94-95 -0.3925337670714022 1101 96-97 -0.4276900570317359 1101 98-99 -0.5204376339642458 1101 100-101 -0.43849966866839907 1101 102-103 -0.2969792708805201 1101 104-105 -0.41749337845906354 1101 106-107 -0.30280430629622046 1101 108-109 -0.24969192676614327 1101 110-111 -0.36270985480578943 1101 112-113 -0.4832064018251394 1101 114-115 -0.7467008714721786 1101 116-117 -0.7381384209951811 1101 118-119 -0.8853674831066414 1101 120-121 -0.9869393990815247 1101 122-123 -0.9527111068267295 1101 124-125 -0.6708540054377679 1102 1 0.2989946983175429 1102 2 0.39853017385005174 1102 3 0.4104747466484575 1102 4 0.31580462345615956 1102 5 0.40431168238400517 1102 6 0.3933173231666913 1102 7 0.36342039490378397 1102 8 0.3869240718497693 1102 9 0.4030037922780565 1102 10-11 0.539310722580197 1102 12-13 0.47129313276560225 1102 14-15 0.4872594434609212 1102 16-17 0.44855561477709216 1102 18-19 0.3992801369544807 1102 20-21 0.47639514961558405 1102 22-23 0.5349587551214299 1102 24-25 0.48261255550607274 1102 26-27 0.5354066747094706 1102 28-29 0.5064581383349847 1102 30-31 0.4223235155140088 1102 32-33 0.47830953806222 1102 34-35 0.5660252283990488 1102 36-37 0.5462921027326288 1102 38-39 0.3960224706909301 1102 40-41 0.4494830494681139 1102 42-43 0.4079248079627362 1102 44-45 0.4019546990672467 1102 46-47 0.477328211423405 1102 48-49 0.474410275656453 1102 50-51 0.37800928087884245 1102 52-53 0.4864304551901739 1102 54-55 0.5769745560319279 1102 56-57 0.5118624962220295 1102 58-59 0.40149587442538603 1102 60-61 0.3222900303521996 1102 62-63 0.3470399586523172 1102 64-65 0.2952066484616651 1102 66-67 0.27740483082729384 1102 68-69 0.37165912003084145 1102 70-71 0.3521872891114697 1102 72-73 0.353798468061683 1102 74-75 0.3627601595960783 1102 76-77 0.3429750444866144 1102 78-79 0.32781639356093706 1102 80-81 0.3764952731505744 1102 82-83 0.3683744685856034 1102 84-85 0.3761914382640086 1102 86-87 0.4420099612448709 1102 88-89 0.43993684682650525 1102 90-91 0.5609587220937868 1102 92-93 0.6729686520250482 1102 94-95 0.46714922568273565 1102 96-97 0.4601490562591728 1102 98-99 0.4994574802195899 1102 100-101 0.4991407888371455 1102 102-103 0.5535750854806096 1102 104-105 0.41444242377154694 1102 106-107 0.37375799890720884 1102 108-109 0.4171702843801235 1102 110-111 0.4804716859693947 1102 112-113 0.44420166724846055 1102 114-115 0.5045053578406318 1102 116-117 0.4545702556968543 1102 118-119 0.41796263666122613 1102 120-121 0.21131741027223327 1102 122-123 0.15346497654408253 1102 124-125 0.008473554356413615 1103 1 0.11228368140617917 1103 2 0.1742853664864228 1103 3 0.1231365502548698 1103 4 0.16505609614143424 1103 5 0.25039997271574777 1103 6 0.16025682886331793 1103 7 0.16305724011584033 1103 8 0.19934492602749998 1103 9 0.2727177244685848 1103 10-11 0.24646658071970506 1103 12-13 0.24969449707508318 1103 14-15 0.3247194554288697 1103 16-17 0.2938156901708453 1103 18-19 0.36056935204309326 1103 20-21 0.23937740317720113 1103 22-23 0.33695301935878774 1103 24-25 0.4343121267738823 1103 26-27 0.42321239469361416 1103 28-29 0.3578607558808642 1103 30-31 0.32938944248328994 1103 32-33 0.3562828561949871 1103 34-35 0.35003793601017463 1103 36-37 0.26986273814748074 1103 38-39 0.3445084993971861 1103 40-41 0.3969960191706008 1103 42-43 0.2504785288960889 1103 44-45 0.22980885216289693 1103 46-47 0.26096859650593984 1103 48-49 0.19564843827907907 1103 50-51 0.021123793131657465 1103 52-53 0.1627819251625482 1103 54-55 0.20215911917816953 1103 56-57 0.09809164545880833 1103 58-59 0.14942697417695427 1103 60-61 0.11136370499781378 1103 62-63 -0.060183597327181815 1103 64-65 0.01882514946122882 1103 66-67 0.07878884783812623 1103 68-69 0.20138314920919953 1103 70-71 0.2106924110408599 1103 72-73 0.07486013608532005 1103 74-75 0.17481898156636078 1103 76-77 0.12446366105271522 1103 78-79 0.09993099530835536 1103 80-81 0.11526377740874238 1103 82-83 0.1933286220866286 1103 84-85 0.2682886899063277 1103 86-87 0.15839748476059157 1103 88-89 0.15416215559070778 1103 90-91 -0.0537173962848172 1103 92-93 0.05300783008408416 1103 94-95 0.06807778684326493 1103 96-97 0.08750705996181907 1103 98-99 0.10826125533304065 1103 100-101 0.09065635668980576 1103 102-103 0.05584566043203765 1103 104-105 -0.04808318899369013 1103 106-107 -0.13355467748993988 1103 108-109 -0.09630161696251704 1103 110-111 -0.05602808312204033 1103 112-113 0.050617776029003636 1103 114-115 0.015564178875422385 1103 116-117 0.018637327386404223 1103 118-119 -0.03913436395962577 1103 120-121 -0.006583881849088868 1103 122-123 -0.3070758515523586 1103 124-125 -0.39096365862548055 1104 1 0.20682551770000046 1104 2 0.21742998745286712 1104 3 0.2142759853517866 1104 4 0.323349419034173 1104 5 0.27930537052976945 1104 6 0.21465111167636053 1104 7 0.15175503501037468 1104 8 0.1486659019870018 1104 9 0.3449232712952863 1104 10-11 0.34239961238467487 1104 12-13 0.20596214222733522 1104 14-15 0.32656567542179715 1104 16-17 0.369441052531684 1104 18-19 0.31230371494406484 1104 20-21 0.35254402325551126 1104 22-23 0.3277756636418303 1104 24-25 0.29371912655679466 1104 26-27 0.4519283365285318 1104 28-29 0.44300425452328795 1104 30-31 0.44835143389081367 1104 32-33 0.41396409515902377 1104 34-35 0.6032423992447562 1104 36-37 0.5434628431139004 1104 38-39 0.3980523320345952 1104 40-41 0.44232238636871557 1104 42-43 0.42342331433793845 1104 44-45 0.2312725572312857 1104 46-47 0.46665924161405314 1104 48-49 0.4849567257921876 1104 50-51 0.402743230414373 1104 52-53 0.3708541310096223 1104 54-55 0.4586583449907806 1104 56-57 0.5377836939223144 1104 58-59 0.4282288617710108 1104 60-61 0.45155293278905617 1104 62-63 0.43697621434576916 1104 64-65 0.42072178095794044 1104 66-67 0.44340303469817144 1104 68-69 0.3629619270169755 1104 70-71 0.3307725229970302 1104 72-73 0.3347569591397388 1104 74-75 0.4010801623965392 1104 76-77 0.4852654591731209 1104 78-79 0.427804683716019 1104 80-81 0.3452258051461783 1104 82-83 0.42474714895981336 1104 84-85 0.35584047128794083 1104 86-87 0.41539887400436015 1104 88-89 0.3310575936048288 1104 90-91 0.34832590596592183 1104 92-93 0.3654259939584037 1104 94-95 0.43287887758126686 1104 96-97 0.48567342032630734 1104 98-99 0.4464434807592319 1104 100-101 0.36392724060853965 1104 102-103 0.5543050920821138 1104 104-105 0.4777302632215168 1104 106-107 0.4180690743449418 1104 108-109 0.40731651184293227 1104 110-111 0.342581291976483 1104 112-113 0.33899784934336097 1104 114-115 0.30896318427656766 1104 116-117 0.2611804631500796 1104 118-119 0.2930968119675157 1104 120-121 0.19118158846261935 1104 122-123 0.1765084333894329 1104 124-125 0.3730753747106661 1105 1 0.07008386923832077 1105 2 0.18191461561598388 1105 3 0.2430370221258613 1105 4 0.22214561419164625 1105 5 0.25619442250501123 1105 6 0.24429281475300257 1105 7 0.212487444440562 1105 8 0.16813300784900775 1105 9 0.3327890777762619 1105 10-11 0.3522170789330019 1105 12-13 0.35941810322314893 1105 14-15 0.3936614393486124 1105 16-17 0.49027723039441184 1105 18-19 0.46582922468373766 1105 20-21 0.4059362667927431 1105 22-23 0.37492274287903626 1105 24-25 0.3928405936512789 1105 26-27 0.5066094363373779 1105 28-29 0.3410062570719887 1105 30-31 0.2825691314149381 1105 32-33 0.34055148479128405 1105 34-35 0.38117311788666086 1105 36-37 0.31094958760689195 1105 38-39 0.33549871265537945 1105 40-41 0.3922108808161937 1105 42-43 0.3561578742323519 1105 44-45 0.3784062671803099 1105 46-47 0.43004744591953425 1105 48-49 0.3332817246543769 1105 50-51 0.38061876518590054 1105 52-53 0.454361833995236 1105 54-55 0.5394055744191419 1105 56-57 0.5497492068478493 1105 58-59 0.4566149895700278 1105 60-61 0.41648671214460364 1105 62-63 0.44525308377049555 1105 64-65 0.36941742200108507 1105 66-67 0.5011181095661854 1105 68-69 0.5082158297113111 1105 70-71 0.4290099103536775 1105 72-73 0.45682538713901266 1105 74-75 0.3675145732375995 1105 76-77 0.4051901075892772 1105 78-79 0.3970017015113996 1105 80-81 0.3557198430125581 1105 82-83 0.33597009748520534 1105 84-85 0.44988758238388016 1105 86-87 0.42617196020497516 1105 88-89 0.5519681559113998 1105 90-91 0.4640460554743875 1105 92-93 0.41760433457373125 1105 94-95 0.39262338195046453 1105 96-97 0.3928804622432942 1105 98-99 0.4170449849463793 1105 100-101 0.3921866446227362 1105 102-103 0.38275977303969455 1105 104-105 0.37108814484358277 1105 106-107 0.40977570664969676 1105 108-109 0.4237332866017134 1105 110-111 0.42475448399359195 1105 112-113 0.3734439625783885 1105 114-115 0.4302374196670655 1105 116-117 0.3230316259936963 1105 118-119 0.28453506292327546 1105 120-121 0.26079746321230246 1105 122-123 0.2876387924828201 1105 124-125 0.35141926124807554 1106 1 -0.15726563128744075 1106 2 -0.06091365725468023 1106 3 -0.09037846157550433 1106 4 -0.7545762575223378 1106 5 -0.1761317473162336 1106 6 -0.08293181721334975 1106 7 -0.020724306348085975 1106 8 0.004162516261210669 1106 9 0.08634783171947902 1106 10-11 0.1296479083231219 1106 12-13 0.13009945837562498 1106 14-15 0.09669525244009236 1106 16-17 0.1723651641483599 1106 18-19 0.01785669576680249 1106 20-21 0.12199091321755873 1106 22-23 0.18848166112299225 1106 24-25 0.1671004188352967 1106 26-27 0.13811985300404217 1106 28-29 0.09381908567345931 1106 30-31 0.042915148765096944 1106 32-33 0.23962893615826886 1106 34-35 0.17777373565743204 1106 36-37 0.264444264263048 1106 38-39 0.22983608778682907 1106 40-41 0.23325977671524356 1106 42-43 0.10427005877494366 1106 44-45 0.16450231739062104 1106 46-47 0.08625883524656075 1106 48-49 0.09452893154186626 1106 50-51 0.1787802399493046 1106 52-53 0.05624307742321122 1106 54-55 0.013561890139442312 1106 56-57 0.11035153334626813 1106 58-59 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-1.0351190476072247 2216 56-57 -0.9619505101197632 2216 58-59 -1.1602594730267057 2216 60-61 -0.9814657814065555 2216 62-63 -0.9761729605831917 2216 64-65 -0.8826159776119837 2216 66-67 -0.9167131547645653 2216 68-69 -1.0654617104148016 2216 70-71 -0.9839583022430602 2216 72-73 -1.044474233096011 2216 74-75 -0.890203209436514 2216 76-77 -1.0033687111983376 2216 78-79 -1.2842686750291534 2216 80-81 -1.3243084602261952 2216 82-83 -1.4008332935294128 2216 84-85 -1.3264086790807212 2216 86-87 -1.2927791181842494 2216 88-89 -1.3477351527013859 2216 90-91 -1.396916299426728 2216 92-93 -1.239891545334551 2216 94-95 -1.0843099263040479 2216 96-97 -1.2615148247300532 2216 98-99 -1.1835734766472008 2216 100-101 -1.0783388014219746 2216 102-103 -1.0438654240081675 2216 104-105 -1.5231811210635513 2216 106-107 -1.3545454186727497 2216 108-109 -1.5092325972475287 2216 110-111 -1.697334357723232 2216 112-113 -1.9240660689492692 2216 114-115 -1.7883271762056765 2216 116-117 -1.9177550428225842 2216 118-119 -2.060116431057807 2216 120-121 -1.7368236316572911 2216 122-123 -1.521355994705484 2216 124-125 -1.2629032075825322 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15561.0 3 3891.0 4 2696.0 5 1974.0 6 2367.0 7 2789.0 8 3253.0 9 3571.0 10 4248.0 11 4423.0 12 4000.0 13 3542.0 14 3323.0 15 3294.0 16 3263.0 17 3256.0 18 3244.0 19 3393.0 20 3473.0 21 3962.0 22 4886.0 23 6637.0 24 9168.0 25 12396.0 26 14700.0 27 14549.0 28 16765.0 29 20798.0 30 25031.0 31 32238.0 32 44564.0 33 61455.0 34 98434.0 35 149292.0 36 308162.0 37 385476.0 38 25154.0 39 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 75.08382247306703 8.737809118079722 5.498050453364259 10.680317955488986 2 10.863988730913903 34.882985452245414 43.11510173650261 11.137924080338072 3 42.713059631948326 18.775187829790614 16.17284007198487 22.338912466276188 4 15.03906951233579 15.440497388618088 49.88950229818414 19.630930800861982 5 17.84111644845836 31.914120054413953 26.588136627602783 23.656626869524903 6 30.36132320963345 39.62067440583347 13.887499226668886 16.130503157864183 7 47.65139936865533 21.25071318422723 15.174956406972134 15.922931040145302 8 45.82525310002177 20.5400740109909 14.825188178099927 18.809484710887407 9 62.106928206612686 9.118509460608232 8.55796851835869 20.216593814420392 10-11 43.19804685202682 24.852952520906673 16.560742581176854 15.388258045889657 12-13 29.532923283262214 30.41196207660599 22.29748463076017 17.757630009371628 14-15 25.576981388879766 30.775828686345303 20.578054505140646 23.069135419634286 16-17 23.356088999145324 35.34352322781151 19.863534745671448 21.436853027371722 18-19 23.266306211624638 26.150621223566397 24.86987033242163 25.71320223238734 20-21 24.245209738854676 27.803487589658673 21.352111213033815 26.599191458452832 22-23 26.34924320480634 22.986914091887776 21.661481847531945 29.00236085577394 24-25 22.24295046632821 22.69549237705563 33.954276845664495 21.10728031095167 26-27 17.977325984608004 22.155054947405084 32.47552801141415 27.39209105657276 28-29 22.055833831429112 23.39001758771419 22.872163479808503 31.6819851010482 30-31 22.133805759082726 28.040757031966645 20.875242787780376 28.950194421170256 32-33 30.425481050141645 24.56492964405383 18.088883050185945 26.920706255618587 34-35 28.932436447458166 17.80685587628535 25.224380590420164 28.036327085836316 36-37 25.124706802487793 25.17859175067652 28.26477482507441 21.43192662176127 38-39 22.572790507083713 29.61777966517245 24.00645091810634 23.802978909637503 40-41 20.530661719384298 29.33277373439112 25.63017893163447 24.506385614590105 42-43 21.918991560952623 21.591060979736053 22.61094150867162 33.87900595063971 44-45 23.919952400992614 23.2760444552732 21.599348034135026 31.204655109599162 46-47 26.13335970933443 20.718667105587766 23.235907615764802 29.912065569313 48-49 17.782452983377773 21.473438272137702 26.446281938574977 34.29782680590955 50-51 23.269026530422856 23.993704891387992 24.86495342253568 27.87231515565347 52-53 25.628689553194107 20.672496373936337 28.331491341364774 25.367322731504782 54-55 21.575403411516792 23.069364554778957 28.1571194962693 27.198112537434955 56-57 20.574956616847327 25.459074716787445 25.96737556367547 27.998593102689757 58-59 22.74330524391054 22.191013166869197 31.63363428961613 23.43204729960413 60-61 25.41364621991933 28.03644165340865 27.596120283730414 18.953791842941605 62-63 27.41109365440768 24.665672729328 24.985392634527155 22.937840981737164 64-65 32.27444808566201 18.218834841780165 27.977217290814473 21.529499781743358 66-67 31.709182757706785 23.19382036846515 23.45931202831504 21.637684845513032 68-69 23.767563541925256 28.462925124601373 24.669925716577236 23.09958561689614 70-71 24.80729564553094 28.359396485867073 21.951222307104658 24.882085561497327 72-73 23.33591111060192 28.2097432342894 22.463858607962088 25.990487047146594 74-75 21.48307822986353 28.698643844594397 25.225758550256582 24.592519375285494 76-77 23.263939410256675 28.355194844128135 22.092136462876677 26.288729282738515 78-79 23.81434846020446 30.11204519233744 18.95472860958023 27.118877737877874 80-81 22.196520533663293 22.412788976916218 25.28610791272119 30.1045825766993 82-83 25.21601715763963 17.92776285429067 28.137413873827494 28.718806114242202 84-85 26.282388936529433 19.580899050223742 32.026004732647436 22.110707280599392 86-87 28.66231651372643 21.963282122414313 24.67790129435254 24.696500069506715 88-89 23.422076208177728 25.074168844845307 26.746735023852057 24.757019923124904 90-91 19.78395561489321 27.773305388965436 25.887747962983898 26.55499103315746 92-93 23.679102448146473 22.639549237933437 26.21502102252588 27.46632729139421 94-95 22.531309453012234 22.129955666983303 27.475126416129932 27.863608463874527 96-97 22.620203848035555 20.259914456696137 27.99070244580214 29.129179249466162 98-99 22.182402014026696 23.066212044136268 29.413168777086778 25.338217164750255 100-101 21.137454730986633 27.678724528005016 25.94984252512781 25.233978215880544 102-103 21.97360210376614 26.42237550514752 25.905713943102686 25.69830844798365 104-105 22.04439070757882 23.113734814423868 25.693893613990493 29.14798086400682 106-107 21.06374682594876 25.511982246317448 22.785458386655865 30.638812541077925 108-109 23.85793315516803 25.788014857122782 24.737621164755044 25.61643082295415 110-111 24.987034769730638 26.992807447808744 22.40498720280217 25.615170579658454 112-113 21.88263797233221 28.272756574766582 23.923313230190153 25.921292222711052 114-115 23.821428748747785 24.77351289604451 25.51959030859381 25.88546804661389 116-117 27.857181591893504 23.941452915401012 23.131918449274444 25.069447043431037 118-119 24.108834718507573 25.527934453346013 24.820161756960967 25.54306907118545 120-121 21.055925978401966 25.878114737601486 26.27082363463675 26.795135649359803 122-123 22.636071088393024 26.87469398947591 27.57409751242565 22.915137409705412 124-125 23.72907441005217 28.72935579791068 22.47148124482926 25.070088547207884 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 2.5 2 4.5 3 14.5 4 18.0 5 15.5 6 20.5 7 22.0 8 24.5 9 27.5 10 31.5 11 49.0 12 70.5 13 89.0 14 104.0 15 139.0 16 192.5 17 219.5 18 263.0 19 356.5 20 430.5 21 519.5 22 650.0 23 784.5 24 930.5 25 1123.5 26 1476.5 27 1841.5 28 1960.0 29 2226.0 30 2633.0 31 2976.5 32 3578.5 33 4160.0 34 4869.5 35 5485.0 36 5889.5 37 6381.5 38 6554.5 39 6535.5 40 6410.5 41 6600.0 42 6959.5 43 7706.0 44 9115.0 45 14559.5 46 36820.5 47 78269.0 48 114503.5 49 145659.5 50 156210.5 51 129709.0 52 96023.5 53 70116.0 54 54287.5 55 47971.5 56 43566.5 57 37185.5 58 31812.0 59 26469.5 60 23708.5 61 20670.0 62 14798.5 63 10128.5 64 7585.5 65 7141.5 66 6904.0 67 6016.5 68 5389.5 69 4883.0 70 4082.5 71 3079.5 72 2351.0 73 1938.0 74 1544.5 75 1317.0 76 1011.5 77 843.5 78 730.5 79 546.0 80 531.0 81 538.0 82 454.0 83 234.0 84 68.5 85 42.0 86 35.5 87 23.0 88 9.0 89 5.0 90 6.5 91 7.0 92 4.0 93 2.0 94 4.0 95 2.5 96 0.5 97 0.5 98 1.0 99 2.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15611741190326525 2 0.015504811456146206 3 0.00817248682663864 4 0.014817406022129873 5 0.003666162314753783 6 0.0 7 1.5275676311474095E-4 8 4.582702893442229E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.001451189249590039 28-29 0.0090508382145484 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.818919077868524E-5 52-53 0.0 54-55 0.0 56-57 5.346486709015933E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0024822974006145403 66-67 1.5275676311474095E-4 68-69 0.007332324629507566 70-71 0.0206985414020474 72-73 2.6732433545079663E-4 74-75 0.009852811220900792 76-77 4.964594801229081E-4 78-79 0.0017185135850408357 80-81 0.0014130000588113538 82-83 0.0 84-85 0.02127137926372768 86-87 0.0034370271700816715 88-89 0.02020208192192449 90-91 2.2913514467211144E-4 92-93 0.015199297929916724 94-95 0.01539024388381015 96-97 0.008363432780532067 98-99 0.004048054222540635 100-101 0.0011074865325818718 102-103 0.0 104-105 8.0197300635239E-4 106-107 0.0016421352034834654 108-109 0.0 110-111 0.0 112-113 0.001756702775819521 114-115 0.006912243530942028 116-117 0.0 118-119 0.07729492213605893 120-121 0.00240591901905717 122-123 0.00641578405081912 124-125 0.2478096589628885 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1309271.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.533110451727794 #Duplication Level Percentage of deduplicated Percentage of total 1 77.92564349473928 22.234609928573594 2 10.215901156163147 5.829828721054735 3 3.4809594695210584 2.979678030654964 4 1.8233652701384062 2.081051305868145 5 1.134202678208337 1.6181165145981982 6 0.7787290627253375 1.3331737415227511 7 0.570292030646953 1.1390543850132748 8 0.44073075725544764 1.0060335500994655 9 0.3524120938684877 0.9049871878986803 >10 2.6103350113613457 15.13986195025466 >50 0.3458964178211176 6.826755723227879 >100 0.27521687690562957 15.812913698088654 >500 0.02677206973727097 5.208892404268979 >1k 0.017134124631853417 9.71500561952984 >5k 8.03162092118129E-4 1.474690461282797 >10k+ 0.001606324184236258 6.695346778063434 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 17939 1.370151786757669 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 16889 1.28995448612243 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 15278 1.166908913433506 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 14401 1.0999250728076921 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 12784 0.9764212298294241 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 10357 0.7910508977896861 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 8136 0.6214145123507662 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 5959 0.45513877570037065 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 5210 0.3979313679139002 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 4767 0.36409574488398505 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 4620 0.3528681227950516 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 4613 0.35233347412415 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 4415 0.33721055457579063 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 3955 0.3020764990594002 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 3593 0.2744275249356321 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 3546 0.2708377410024357 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 3509 0.268011740884813 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 3306 0.2525069294286668 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 3250 0.24822974006145404 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 3203 0.24463995612825765 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 3195 0.24402892907579868 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 2752 0.21019330604588357 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 2645 0.2020208192192449 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2424 0.18514119689506603 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 2396 0.18300260221145967 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 2385 0.1821624400143286 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 2363 0.1804821156200664 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 2361 0.1803293588569517 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 2234 0.17062930439916565 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 2206 0.16849070971555927 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2166 0.16543557445326446 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 2080 0.15886703363933058 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2030 0.15504811456146206 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1954 0.1492433575631019 No Hit CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA 1807 0.13801573547416845 No Hit ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA 1765 0.13480784344875887 No Hit GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1703 0.13007238379220193 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1695 0.12946135673974296 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1680 0.1283156810163824 No Hit CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 1670 0.1275518972008087 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA 1584 0.12098335638687484 No Hit GAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGA 1580 0.12067784286064534 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1568 0.1197613022819569 No Hit ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC 1547 0.11815735626925213 No Hit GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG 1503 0.11479670748072782 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 1426 0.10891557210081029 No Hit ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA 1410 0.10769351799589236 No Hit GAGTAAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1346 0.10280530157622066 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCACGT 1332 0.10173600423441748 No Hit GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1325 0.10120135556351588 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC 1320 0.10081946365572902 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.637838155737048E-5 0.0 3 0.0 0.0 0.0 7.637838155737048E-5 0.0 4 0.0 0.0 0.0 7.637838155737048E-5 0.0 5 0.0 0.0 0.0 7.637838155737048E-5 0.0 6 0.0 0.0 0.0 7.637838155737048E-5 0.0 7 0.0 0.0 0.0 7.637838155737048E-5 0.0 8 0.0 0.0 0.0 7.637838155737048E-5 0.0 9 0.0 0.0 0.0 7.637838155737048E-5 0.0 10-11 0.0 0.0 0.0 1.1456757233605571E-4 0.0 12-13 0.0 0.0 0.0 1.5275676311474095E-4 0.0 14-15 0.0 0.0 0.0 1.5275676311474095E-4 0.0 16-17 0.0 0.0 0.0 1.5275676311474095E-4 0.0 18-19 0.0 0.0 0.0 1.5275676311474095E-4 0.0 20-21 0.0 0.0 0.0 7.637838155737048E-4 0.0 22-23 0.0 0.0 0.0 0.0021385946836063735 0.0 24-25 0.0 0.0 0.0 0.0038189190778685237 0.0 26-27 0.0 0.0 0.0 0.006568540813933861 0.0 28-29 0.0 0.0 0.0 0.015657568219260946 0.0 30-31 0.0 0.0 0.0 0.05060067778175794 0.0 32-33 0.0 0.0 0.0 0.11945578875572742 0.0 34-35 0.0 0.0 0.0 0.1991566299108435 0.0 36-37 0.0 0.0 0.0 0.30051074223747415 0.0 38-39 0.0 0.0 0.0 0.45704823523930493 0.0 40-41 0.0 0.0 0.0 0.7127248675026026 0.0 42-43 0.0 0.0 0.0 1.3025569190793962 0.0 44-45 0.0 0.0 0.0 2.165059792816002 0.0 46-47 0.0 0.0 0.0 3.011485017234782 0.0 48-49 0.0 0.0 0.0 3.3242926789029927 0.0 50-51 0.0 0.0 0.0 3.7360103446879984 0.0 52-53 0.0 0.0 0.0 4.034917140912768 0.0 54-55 3.818919077868524E-5 0.0 0.0 4.3348550452885615 0.0 56-57 7.637838155737048E-5 0.0 0.0 4.824516849452864 0.0 58-59 7.637838155737048E-5 0.0 0.0 5.389640494595847 0.0 60-61 7.637838155737048E-5 0.0 0.0 6.2106699071468014 0.0 62-63 7.637838155737048E-5 0.0 0.0 7.223829138505321 0.0 64-65 7.637838155737048E-5 0.0 0.0 8.148504014829626 0.0 66-67 7.637838155737048E-5 0.0 0.0 8.875282504538784 0.0 68-69 7.637838155737048E-5 0.0 0.0 9.44422506875964 0.0 70-71 7.637838155737048E-5 0.0 0.0 10.149235719724945 0.0 72-73 7.637838155737048E-5 0.0 0.0 10.845424667620378 0.0 74-75 7.637838155737048E-5 0.0 0.0 11.617266402448386 0.0 76-77 7.637838155737048E-5 0.0 0.0 12.587386415799326 0.0 78-79 1.1456757233605571E-4 0.0 0.0 13.788932925269101 0.0 80-81 1.5275676311474095E-4 0.0 0.0 16.184769997960696 0.0 82-83 1.5275676311474095E-4 0.0 0.0 17.839545823591905 0.0 84-85 1.5275676311474095E-4 0.0 0.0 18.66496699308241 0.0 86-87 1.5275676311474095E-4 0.0 0.0 19.366311481732964 0.0 88-89 1.5275676311474095E-4 0.0 0.0 20.11199362087757 0.0 90-91 1.5275676311474095E-4 0.0 0.0 21.026586550836306 0.0 92-93 1.5275676311474095E-4 0.0 0.0 22.30802484741509 0.0 94-95 1.5275676311474095E-4 0.0 0.0 24.117352328127637 0.0 96-97 1.5275676311474095E-4 0.0 0.0 26.24021306513319 0.0 98-99 1.5275676311474095E-4 0.0 0.0 28.25148498668343 0.0 100-101 1.5275676311474095E-4 0.0 0.0 29.850351837014642 0.0 102-103 1.5275676311474095E-4 0.0 0.0 31.2662160851344 0.0 104-105 1.5275676311474095E-4 0.0 0.0 32.86084393528918 0.0 106-107 1.5275676311474095E-4 0.0 0.0 34.34743456473106 0.0 108-109 1.5275676311474095E-4 0.0 0.0 35.489673260921535 0.0 110-111 1.5275676311474095E-4 0.0 0.0 36.65891171499254 0.0 112-113 1.5275676311474095E-4 0.0 0.0 39.80447898105129 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGT 3255 0.0 95.35146 1 AGTAGTA 2765 0.0 94.67106 2 GAGTTAG 1580 0.0 93.50508 1 GAGTCCG 905 0.0 92.15525 1 GAGTACG 820 0.0 90.81065 1 AGTCCGT 395 0.0 88.861694 2 AGTCGTA 595 0.0 87.98839 2 GAGTAGG 1380 0.0 87.19928 1 GAGTCAG 7105 0.0 86.44408 1 GAGTAAG 1235 0.0 86.34291 1 AGTCGGT 395 0.0 85.849434 2 GAGTCGT 1085 0.0 85.102554 1 GAGTTCG 515 0.0 84.44156 1 GAGTCGG 990 0.0 84.24293 1 AGTTCGT 100 0.0 83.28901 2 AGTTGTA 555 0.0 82.53866 2 GAGTTGC 470 0.0 82.38651 1 AGTTAGT 1135 0.0 82.29311 2 AGTCCGG 370 0.0 82.00269 2 GAGTTAC 820 0.0 79.913376 1 >>END_MODULE