##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935928_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1141461 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.009224143444236 31.0 31.0 34.0 28.0 34.0 2 31.065825288818452 33.0 31.0 34.0 28.0 34.0 3 31.284721948450276 34.0 31.0 34.0 28.0 34.0 4 34.4235475412651 37.0 35.0 37.0 31.0 37.0 5 34.51674564439784 37.0 35.0 37.0 31.0 37.0 6 34.69001306220712 37.0 35.0 37.0 32.0 37.0 7 34.62914545481624 37.0 35.0 37.0 32.0 37.0 8 34.56624010807202 37.0 35.0 37.0 31.0 37.0 9 36.15286987466063 39.0 37.0 39.0 31.0 39.0 10-11 35.95564544036108 39.0 37.0 39.0 31.0 39.0 12-13 35.794944373920785 39.0 37.0 39.0 30.0 39.0 14-15 37.20730449835781 40.0 38.0 41.0 31.0 41.0 16-17 37.35391704140571 40.0 38.0 41.0 31.0 41.0 18-19 37.421805037579034 40.0 38.0 41.0 31.0 41.0 20-21 37.52913327744005 40.0 38.0 41.0 31.0 41.0 22-23 37.437902390007196 40.0 38.0 41.0 31.0 41.0 24-25 37.409671026868196 40.0 37.5 41.0 31.0 41.0 26-27 37.38418307765224 40.0 37.0 41.0 30.5 41.0 28-29 37.29075500608431 40.0 37.0 41.0 30.0 41.0 30-31 37.21020078653585 40.0 37.0 41.0 30.0 41.0 32-33 37.121961240900916 40.0 36.5 41.0 30.0 41.0 34-35 36.917591577811244 40.0 36.0 41.0 30.0 41.0 36-37 36.915250718158575 40.0 36.0 41.0 30.0 41.0 38-39 37.00657490707085 40.0 36.0 41.0 30.0 41.0 40-41 36.970295086735334 40.0 36.0 41.0 30.0 41.0 42-43 36.81732402596322 40.0 35.0 41.0 30.0 41.0 44-45 36.64374910750345 40.0 35.0 41.0 30.0 41.0 46-47 36.486903626142286 40.0 35.0 41.0 30.0 41.0 48-49 36.3161452734697 39.5 35.0 41.0 30.0 41.0 50-51 36.09130272519166 39.0 35.0 41.0 30.0 41.0 52-53 35.84876005400097 39.0 35.0 41.0 29.5 41.0 54-55 35.58318987683329 38.5 35.0 41.0 29.0 41.0 56-57 35.301957754141405 38.0 35.0 40.0 29.0 41.0 58-59 34.93843416463638 37.0 34.0 40.0 28.0 41.0 60-61 34.625971014340394 37.0 34.0 39.5 28.0 41.0 62-63 34.225743148473754 36.0 34.0 39.0 27.0 41.0 64-65 33.82789994577125 35.5 34.0 39.0 27.0 41.0 66-67 33.528197634435166 35.0 33.5 38.0 26.5 40.0 68-69 33.3302968739186 35.0 34.0 37.0 27.0 40.0 70-71 33.12843802810609 35.0 34.0 37.0 27.0 39.0 72-73 32.86421787516174 35.0 34.0 36.0 27.0 39.0 74-75 32.565660149580225 35.0 33.0 36.0 26.0 38.5 76-77 32.3639265818105 35.0 33.0 35.0 26.5 37.0 78-79 32.15999933418662 35.0 33.0 35.0 26.0 37.0 80-81 31.95731566825323 35.0 33.0 35.0 26.0 36.0 82-83 31.80761716782264 35.0 33.0 35.0 25.5 36.0 84-85 31.60420417342336 35.0 33.0 35.0 25.0 36.0 86-87 31.434615374506883 35.0 33.0 35.0 25.0 35.0 88-89 31.312841612635033 35.0 33.0 35.0 24.0 35.0 90-91 31.237979659401418 35.0 33.0 35.0 24.0 35.0 92-93 31.136293749852165 35.0 33.0 35.0 23.0 36.0 94-95 30.984306515947544 35.0 33.0 35.0 21.5 35.0 96-97 30.880287631377684 35.0 32.5 35.0 20.0 35.0 98-99 30.727064700414644 35.0 32.0 35.0 19.5 35.0 100-101 30.612985025331568 35.0 32.0 35.0 18.5 35.0 102-103 29.93136296378063 34.0 30.5 35.0 17.5 35.0 104-105 30.430125076546638 35.0 32.0 35.0 18.0 35.0 106-107 30.455504393054163 35.0 32.0 35.0 17.0 35.0 108-109 30.3614113841822 35.0 32.0 35.0 15.0 35.0 110-111 30.26181183588401 35.0 32.0 35.0 11.5 35.0 112-113 30.173232813035224 35.0 32.0 35.0 6.0 35.0 114-115 30.005561293815557 35.0 31.0 35.0 2.0 35.0 116-117 29.828546047565357 35.0 31.0 35.0 2.0 35.0 118-119 29.58348073214941 35.0 31.0 35.0 2.0 35.0 120-121 29.343206644817474 35.0 31.0 35.0 2.0 35.0 122-123 28.925437224749686 35.0 30.5 35.0 2.0 35.0 124-125 26.169309332513333 32.0 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17936411578375555 1101 2 0.34456645207772496 1101 3 0.2514173122105099 1101 4 0.3667684929867434 1101 5 0.24646480848836916 1101 6 0.29280015783722746 1101 7 0.20193588933170048 1101 8 0.2211880139028679 1101 9 0.15808165352936498 1101 10-11 0.3278091695762839 1101 12-13 0.26601729560389487 1101 14-15 0.3978193898906568 1101 16-17 0.4642059763658679 1101 18-19 0.42975735469543963 1101 20-21 0.43525667471260476 1101 22-23 0.4850198426354808 1101 24-25 0.45266412585885263 1101 26-27 0.47308661092418447 1101 28-29 0.40229023146527965 1101 30-31 0.34122672059856995 1101 32-33 0.35859841863148034 1101 34-35 0.1718168898038428 1101 36-37 0.27059831810247914 1101 38-39 0.3791740191462267 1101 40-41 0.3763710895519168 1101 42-43 0.3470178458719815 1101 44-45 0.33136687262742726 1101 46-47 0.35579348238954367 1101 48-49 0.35242839338085474 1101 50-51 0.4842855824500347 1101 52-53 0.4627021127240738 1101 54-55 0.2849756068404403 1101 56-57 0.24286523399205606 1101 58-59 0.17830454450318456 1101 60-61 0.27905138659219375 1101 62-63 0.390633748960191 1101 64-65 0.3767370375865653 1101 66-67 0.43234100810371956 1101 68-69 0.6210986948659141 1101 70-71 0.45030774509752547 1101 72-73 0.5408193426980148 1101 74-75 0.6077302029155547 1101 76-77 0.46710430937206837 1101 78-79 0.5706547482966258 1101 80-81 0.6143928381152008 1101 82-83 0.5688618985016092 1101 84-85 0.5603624605018176 1101 86-87 0.4815870724521396 1101 88-89 0.3639730269653896 1101 90-91 0.42703925422597067 1101 92-93 0.0804508962525432 1101 94-95 0.14856570199416197 1101 96-97 0.25618434710456484 1101 98-99 0.1073625919353205 1101 100-101 0.11998346249161784 1101 102-103 0.08565713036670886 1101 104-105 0.09110422845716215 1101 106-107 0.21412535260468601 1101 108-109 0.2023126252219356 1101 110-111 0.1067174169140408 1101 112-113 0.05512785837925094 1101 114-115 0.04849511199459755 1101 116-117 0.0040004139368754466 1101 118-119 0.12622482456327688 1101 120-121 0.08770043457273147 1101 122-123 4.7719218641262273E-4 1101 124-125 0.27311737634786226 1102 1 0.2812926774259559 1102 2 0.4552309215692958 1102 3 0.36518522867763537 1102 4 0.34931427480021426 1102 5 0.5213907418164254 1102 6 0.5011773278304474 1102 7 0.46344497656131267 1102 8 0.5968157108787366 1102 9 0.6491427458069481 1102 10-11 0.7601643326235177 1102 12-13 0.8853988184674719 1102 14-15 0.7573327935386232 1102 16-17 0.7463167225380971 1102 18-19 0.739690396161464 1102 20-21 0.6469836568523846 1102 22-23 0.8214522235663111 1102 24-25 0.6805192279432788 1102 26-27 0.7229800831270268 1102 28-29 0.6225042423531093 1102 30-31 0.6087026214340696 1102 32-33 0.9012491318963569 1102 34-35 0.7997966828319534 1102 36-37 0.8456510792149956 1102 38-39 0.7336275095499261 1102 40-41 0.6704827105718891 1102 42-43 0.5872402281477633 1102 44-45 0.5706986950886375 1102 46-47 0.45999903641579465 1102 48-49 0.5983259733458368 1102 50-51 0.5144294923909953 1102 52-53 0.6071362081553886 1102 54-55 0.6446046512829682 1102 56-57 0.5488752871993867 1102 58-59 0.5339446100162064 1102 60-61 0.36122234139549647 1102 62-63 0.4378472457492677 1102 64-65 0.427563680722848 1102 66-67 0.3367110988691735 1102 68-69 0.5611508840592876 1102 70-71 0.5928683339605669 1102 72-73 0.5303827609896175 1102 74-75 0.5552129416415212 1102 76-77 0.6065407113344392 1102 78-79 0.5794076251756337 1102 80-81 0.5915544096735985 1102 82-83 0.563747307272962 1102 84-85 0.580706441587008 1102 86-87 0.5265323756132858 1102 88-89 0.5380249924189577 1102 90-91 0.5153253445238306 1102 92-93 0.7111042541721133 1102 94-95 0.7266961422964897 1102 96-97 0.6794390824065566 1102 98-99 0.6896083361655343 1102 100-101 0.6395493821438905 1102 102-103 0.6668300548344135 1102 104-105 0.5915982157643285 1102 106-107 0.5009321206078852 1102 108-109 0.5988417673306472 1102 110-111 0.5741745889686882 1102 112-113 0.5228100271972131 1102 114-115 0.4799729133614221 1102 116-117 0.38740244041609984 1102 118-119 0.2593481190186786 1102 120-121 0.22826936214262616 1102 122-123 0.11970662226583784 1102 124-125 -0.06609666847803197 1103 1 0.22117947919246106 1103 2 0.37303160737231167 1103 3 0.35188970796336605 1103 4 0.35865241019239136 1103 5 0.32889982954505115 1103 6 0.14196463544257654 1103 7 0.1606576737416603 1103 8 0.24535843937790247 1103 9 0.24798033126274532 1103 10-11 0.4018196770705984 1103 12-13 0.3798766904173547 1103 14-15 0.44676790464015426 1103 16-17 0.36669647816985673 1103 18-19 0.3359232720531651 1103 20-21 0.4130048970967053 1103 22-23 0.39188601434128145 1103 24-25 0.4577326919194533 1103 26-27 0.5414172094638303 1103 28-29 0.4298527822061189 1103 30-31 0.4282336299383189 1103 32-33 0.4058870485121062 1103 34-35 0.4584539274949364 1103 36-37 0.5311677438448683 1103 38-39 0.44860650653306067 1103 40-41 0.5759607478621334 1103 42-43 0.425549347506724 1103 44-45 0.3770072681055723 1103 46-47 0.5084844948518707 1103 48-49 0.44795731229115887 1103 50-51 0.23201619423442565 1103 52-53 0.36838055004088943 1103 54-55 0.4400684549257221 1103 56-57 0.4087954685466073 1103 58-59 0.5629870018440783 1103 60-61 0.46539814371553945 1103 62-63 0.3248496970996655 1103 64-65 0.3262819335785636 1103 66-67 0.375602623618704 1103 68-69 0.45141701013488955 1103 70-71 0.4008932116925763 1103 72-73 0.5390086149485569 1103 74-75 0.5473311192553965 1103 76-77 0.4891328885792916 1103 78-79 0.5800450242042032 1103 80-81 0.467861856459173 1103 82-83 0.4604113860037202 1103 84-85 0.5069886080727564 1103 86-87 0.4470072618519332 1103 88-89 0.4001299428048739 1103 90-91 0.3426800666681764 1103 92-93 0.4550753220071897 1103 94-95 0.39310102007642556 1103 96-97 0.38327437945606846 1103 98-99 0.45940964019768415 1103 100-101 0.47477634172656025 1103 102-103 0.4718063201074294 1103 104-105 0.33789483155888433 1103 106-107 0.35452144393453366 1103 108-109 0.447468483934891 1103 110-111 0.4029299116100127 1103 112-113 0.4044922334604948 1103 114-115 0.3238017645335418 1103 116-117 0.3151445142633307 1103 118-119 0.30003322712491 1103 120-121 0.255341448138779 1103 122-123 0.29624758234213644 1103 124-125 0.3815605306564471 1104 1 0.269411786573599 1104 2 0.35362499971009953 1104 3 0.3575076854914556 1104 4 0.2877354697757397 1104 5 0.3706039842678521 1104 6 0.23711106553345473 1104 7 0.23300787844388537 1104 8 0.18968431694713672 1104 9 0.32189282890885096 1104 10-11 0.4281397797355595 1104 12-13 0.4016790464109121 1104 14-15 0.583198274499054 1104 16-17 0.5765737963266275 1104 18-19 0.39012925758800066 1104 20-21 0.5530934037817872 1104 22-23 0.38516606232599315 1104 24-25 0.3118535882850253 1104 26-27 0.45468902751250084 1104 28-29 0.5391330812736328 1104 30-31 0.5498867311595603 1104 32-33 0.4660144032851221 1104 34-35 0.5588905325765765 1104 36-37 0.463574163194977 1104 38-39 0.5050949044155999 1104 40-41 0.5810835343231915 1104 42-43 0.4900497276181781 1104 44-45 0.3068540378556719 1104 46-47 0.44251249058346076 1104 48-49 0.5390341087536754 1104 50-51 0.613254032411561 1104 52-53 0.4528228323834895 1104 54-55 0.5203783329413341 1104 56-57 0.521896062078433 1104 58-59 0.5574806166691246 1104 60-61 0.5347998692122999 1104 62-63 0.41557951108916313 1104 64-65 0.41617306128903664 1104 66-67 0.42907454070558515 1104 68-69 0.5906023162117933 1104 70-71 0.6178549006428398 1104 72-73 0.5431601992112505 1104 74-75 0.5161532619680642 1104 76-77 0.6002666668871299 1104 78-79 0.45090826080090807 1104 80-81 0.4754780345605667 1104 82-83 0.5021416951842852 1104 84-85 0.5226185120335849 1104 86-87 0.48463829568533967 1104 88-89 0.38038158356118856 1104 90-91 0.5359649968183398 1104 92-93 0.47574954905577727 1104 94-95 0.4370666635807048 1104 96-97 0.4027455014474377 1104 98-99 0.45287276938675447 1104 100-101 0.387533788772501 1104 102-103 0.3145238728871256 1104 104-105 0.3653448067993317 1104 106-107 0.49437630413023825 1104 108-109 0.42100191630643735 1104 110-111 0.29406459660572537 1104 112-113 0.3870977754157323 1104 114-115 0.418306538593896 1104 116-117 0.25990804309251914 1104 118-119 0.2760286657874751 1104 120-121 0.22425425963837142 1104 122-123 0.21317928084749482 1104 124-125 0.3547766222737678 1105 1 -0.11675093282289595 1105 2 -0.06612175954601796 1105 3 -0.11717842224447139 1105 4 0.004806291352487335 1105 5 -0.1542136237173466 1105 6 -0.06018471310867568 1105 7 -0.04978704678538293 1105 8 -0.10433809730240284 1105 9 -0.12941851556146844 1105 10-11 -0.026547127485670785 1105 12-13 -0.10048390076347147 1105 14-15 0.0018327309584122986 1105 16-17 -0.10333566573289232 1105 18-19 -0.061752097283161334 1105 20-21 -0.10498256693291808 1105 22-23 -0.05602387351154192 1105 24-25 -0.01533294747723346 1105 26-27 0.07718469633853431 1105 28-29 0.05532360164051653 1105 30-31 0.016377171653338962 1105 32-33 0.08383138601246998 1105 34-35 0.0018485754365329399 1105 36-37 0.03512998423999392 1105 38-39 -0.0012636436285689001 1105 40-41 0.02696139559438393 1105 42-43 0.025997905885297712 1105 44-45 0.037076015706915655 1105 46-47 0.11123462644618343 1105 48-49 0.08187709360947082 1105 50-51 0.06812817758451928 1105 52-53 -0.0040639793520469425 1105 54-55 0.0032306928937586576 1105 56-57 7.032903307333527E-4 1105 58-59 -0.011322071109987064 1105 60-61 0.007417308702194703 1105 62-63 -0.12811424681611783 1105 64-65 -0.23340196701349925 1105 66-67 -0.1416240963228006 1105 68-69 -0.09418098932810892 1105 70-71 -0.11114861633830486 1105 72-73 0.003993149772135496 1105 74-75 0.0385211262788161 1105 76-77 0.07331722377390548 1105 78-79 0.05640317614975032 1105 80-81 0.03640277508610268 1105 82-83 0.06912687378251547 1105 84-85 -0.013032310493496624 1105 86-87 0.007790183564310382 1105 88-89 0.09039977562320445 1105 90-91 0.022530815200919818 1105 92-93 -0.0472554522528128 1105 94-95 -0.07649121199071729 1105 96-97 0.005468993418265455 1105 98-99 -0.015030723685253378 1105 100-101 -0.0474729578276083 1105 102-103 0.07996016182715238 1105 104-105 0.03377467798414813 1105 106-107 0.041572335666163696 1105 108-109 -0.046358718696083656 1105 110-111 -0.014567657497860154 1105 112-113 -0.04660056821662906 1105 114-115 0.0267770854795657 1105 116-117 -0.003363790210269002 1105 118-119 -0.053520122803718095 1105 120-121 0.22661292986802906 1105 122-123 0.2009516809551961 1105 124-125 0.14482159709375253 1106 1 -0.1416914002932863 1106 2 0.009177913850912489 1106 3 0.0507700968389706 1106 4 -0.7326430449567525 1106 5 -0.10794772802925223 1106 6 -0.06323099767560336 1106 7 0.07942942182251755 1106 8 -0.03886739534996764 1106 9 0.20231268178204687 1106 10-11 0.0770660446430469 1106 12-13 0.039295103571127754 1106 14-15 0.05042822285939508 1106 16-17 0.1339090050644245 1106 18-19 0.041158748983590954 1106 20-21 0.10882395306398251 1106 22-23 0.076425951122431 1106 24-25 -0.019284069044239516 1106 26-27 -0.08960584682219519 1106 28-29 -0.1339736847102273 1106 30-31 -0.06566030583345395 1106 32-33 0.07825802096675005 1106 34-35 0.1487811780062671 1106 36-37 0.01052200846090301 1106 38-39 0.10583351001275787 1106 40-41 0.06445252675553803 1106 42-43 -0.08030105225952866 1106 44-45 0.0642599044378187 1106 46-47 0.1581245724144935 1106 48-49 0.10679134953183933 1106 50-51 0.11661523267041929 1106 52-53 0.046492351778077534 1106 54-55 0.14502834548805055 1106 56-57 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2216 62-63 -0.9276688024750186 2216 64-65 -0.7667237168299792 2216 66-67 -0.6743987353950445 2216 68-69 -0.8880555133868526 2216 70-71 -0.7547727019569237 2216 72-73 -0.8167362450452202 2216 74-75 -0.7219159129983268 2216 76-77 -0.9241498338464709 2216 78-79 -1.2154620145604298 2216 80-81 -1.2649168139435467 2216 82-83 -1.3995955264344033 2216 84-85 -1.298287886363628 2216 86-87 -1.2511462091000745 2216 88-89 -1.2501708007609693 2216 90-91 -1.3565025483337294 2216 92-93 -1.2339790885792823 2216 94-95 -1.192956209228111 2216 96-97 -1.3129330764640095 2216 98-99 -1.210332876115114 2216 100-101 -1.3388202511024154 2216 102-103 -1.2400836912032709 2216 104-105 -1.6011628221285825 2216 106-107 -1.4758546445891518 2216 108-109 -1.674290345484792 2216 110-111 -1.7643727117189414 2216 112-113 -1.8093136783728667 2216 114-115 -1.661569996766815 2216 116-117 -1.5842515585575683 2216 118-119 -1.7590571540846263 2216 120-121 -1.6023270340467093 2216 122-123 -1.524460049940867 2216 124-125 -1.356791177160538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16492.0 3 3658.0 4 2534.0 5 1768.0 6 1962.0 7 2193.0 8 2542.0 9 3145.0 10 3948.0 11 4457.0 12 4546.0 13 4101.0 14 4048.0 15 4142.0 16 4020.0 17 3718.0 18 3645.0 19 3426.0 20 3389.0 21 3668.0 22 4266.0 23 5633.0 24 8301.0 25 12838.0 26 15556.0 27 13318.0 28 14742.0 29 18839.0 30 21061.0 31 25731.0 32 35032.0 33 47381.0 34 78355.0 35 113546.0 36 228949.0 37 349432.0 38 66924.0 39 155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 65.35256275269542 11.356553234501348 7.647721529649595 15.643162483153638 2 12.459365443760023 30.0791223812069 41.76084537401316 15.700666801019917 3 34.935879744622916 20.47301009049643 20.387236052081224 24.203874112799433 4 14.197529782454025 15.878718206638883 45.284926696084035 24.638825314823055 5 16.78559757635014 33.61543832720499 28.3428784696943 21.25608562675056 6 31.42279937728928 37.800765860594446 15.215149707261133 15.561285054855137 7 41.915755334610644 24.69860993936718 16.8272941432077 16.55834058281448 8 39.57876680526134 25.879445411044472 16.096693877479503 18.445093906214684 9 50.84001993935841 11.877672561743239 11.85349302341473 25.428814475483613 10-11 37.90694557238486 24.79616035939905 20.2104145476718 17.086479520544284 12-13 29.278310866512303 27.337245862977362 23.867087881232912 19.517355389277427 14-15 25.246329046721698 27.683950656220407 22.48578795070528 24.583932346352615 16-17 24.055661998088414 31.716458118148584 21.705472197473238 22.522407686289764 18-19 24.515248440375974 25.726108907794487 24.731199751896913 25.027442899932627 20-21 25.047461104671996 26.86123310389054 22.567788124167183 25.52351766727028 22-23 25.499776164056414 24.402629612400247 22.6562274138144 27.441366809728933 24-25 23.027243155920353 23.850442546876327 30.732149412025468 22.390164885177853 26-27 20.45430950990695 23.440870859913275 29.466646049552036 26.63817358062774 28-29 22.778117040766173 24.47713829868324 23.6785755276805 29.066169132870083 30-31 23.42962221223502 27.26518908661794 22.58828816753266 26.716900533614375 32-33 28.705886578691693 24.75340813220951 21.03059149633671 25.510113792762084 34-35 27.62976571253858 20.576787117562493 25.73140913268171 26.062038037217217 36-37 25.06012031948529 25.35710812721591 27.339173217481804 22.24359833581699 38-39 23.49300589332443 28.30057268710889 24.399431956063324 23.806989463503353 40-41 22.003555092990474 27.680840606906408 26.032383059955617 24.283221240147494 42-43 22.653292578546267 22.822987381960488 24.21541340440015 30.308306635093096 44-45 23.48792468599453 24.101699488637806 23.515433291194356 28.894942534173307 46-47 24.68301588928575 22.78106742148878 24.19206613278947 28.343850556436006 48-49 19.84837852541611 23.310827089142773 25.716691152829576 31.12410323261154 50-51 23.362525466277635 24.87247697138885 24.930472845972332 26.834524716361187 52-53 24.476263315172396 22.925969437413983 27.51504431601255 25.082722931401076 54-55 21.90828245555477 24.678416520581955 27.299618646629188 26.11368237723409 56-57 21.903247962686187 26.086990802958333 25.567083737058173 26.442677497297307 58-59 23.67794431872837 23.380430868860174 28.89691369218922 24.044711120222242 60-61 25.337484154079725 27.51381781769154 26.589038083648937 20.559659944579796 62-63 26.14925082854342 25.326796097282344 25.16288335738146 23.361069716792777 64-65 29.198977953638206 21.298312519629885 26.660639168836088 22.842070357895825 66-67 28.925699477598403 24.521599111312803 23.762220106022205 22.79048130506659 68-69 24.004913337591326 27.75904319584224 24.937641440252744 23.298402026313692 70-71 24.885716313585498 27.401578459901916 23.351331316227363 24.361373910285224 72-73 23.696493963870832 27.721952587037652 23.477257196923237 25.10429625216827 74-75 22.161115516206614 27.825829825076337 25.228478431338193 24.78457622737886 76-77 23.27719015135487 27.82256704482387 23.18064683148241 25.719595972338848 78-79 24.000488853826134 28.93559088016029 20.814221966795614 26.249698299217965 80-81 23.227053030057462 23.994543767691358 24.365783080598575 28.412620121652605 82-83 25.00843217595695 20.906233327288447 26.682208152534344 27.403126344220258 84-85 25.941923962608698 21.901539394406726 29.357367677624453 22.79916896536012 86-87 27.41250789031142 23.757141799573606 24.61820967793512 24.212140632179853 88-89 23.873787828470334 25.84152108633001 26.10671578296182 24.177975302237833 90-91 21.469415074190007 27.637256112999044 25.666448525179575 25.226880287631374 92-93 24.306861941916857 23.705877971952912 26.195812651417903 25.79144743471232 94-95 23.14973503806199 23.582488679536244 27.096081998009282 26.171694284392483 96-97 23.228035499085976 22.610625807409704 27.116700244396814 27.044638449107506 98-99 22.40823742973689 24.57570543461501 28.15759086894966 24.85846626669844 100-101 21.44417188663542 28.13412764466249 25.577029217223707 24.844671251478385 102-103 22.728513163618015 26.873904090417494 25.24629630197453 25.151286443989957 104-105 23.011941796990325 24.160959759184127 25.017061587292687 27.81003685653286 106-107 22.161290576927627 25.610608676911596 23.404234815054277 28.8238659311065 108-109 23.86914664627175 25.87122994127701 24.99682424541881 25.262799167032423 110-111 24.87837955041828 27.04682858196644 23.327384816476428 24.747407051138847 112-113 22.551829086665663 27.93671875273776 24.368343957303207 25.143108203293373 114-115 23.73551816146415 25.469155277375364 25.422895750037345 25.372430811123138 116-117 26.343168973797614 24.56290666084956 24.164119492474995 24.929804872877828 118-119 23.5079570073481 25.677711173477753 25.58907104974982 25.22526076942433 120-121 21.499021409835663 26.285834184598727 26.681869711714523 25.533274693851087 122-123 22.62826513684345 26.951175118528152 27.40355994044133 23.01699980418707 124-125 23.362417896382016 28.20372631966319 23.766387238802135 24.667468545152655 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 2.0 3 8.5 4 12.0 5 11.5 6 18.0 7 28.0 8 43.5 9 48.0 10 59.5 11 89.0 12 110.0 13 133.0 14 168.0 15 218.5 16 270.0 17 330.0 18 418.5 19 513.0 20 638.0 21 791.5 22 1000.5 23 1216.0 24 1459.0 25 1749.0 26 2110.5 27 2530.5 28 2843.5 29 3283.0 30 4036.5 31 4787.5 32 5558.5 33 6500.5 34 7708.0 35 8747.0 36 9830.0 37 11141.0 38 11975.0 39 12526.0 40 13064.5 41 14454.0 42 15905.5 43 17620.5 44 19784.0 45 23690.0 46 37644.0 47 63590.0 48 85066.5 49 100540.0 50 102809.0 51 84379.5 52 63808.0 53 50298.0 54 43257.0 55 39763.0 56 36614.0 57 32596.0 58 29338.5 59 25515.0 60 22193.0 61 19339.5 62 15614.0 63 12435.5 64 10421.0 65 9658.5 66 8650.5 67 7506.0 68 6852.0 69 6177.5 70 5105.5 71 3603.0 72 2516.5 73 1894.0 74 1450.0 75 1171.0 76 904.0 77 744.5 78 609.0 79 447.0 80 441.5 81 430.5 82 309.5 83 147.0 84 49.5 85 30.0 86 26.0 87 15.5 88 8.5 89 12.0 90 11.5 91 8.0 92 6.5 93 5.5 94 3.5 95 2.0 96 1.0 97 0.5 98 1.0 99 1.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1532246831034963 2 0.01673294137951275 3 0.00788463206364475 4 0.01620729924193643 5 0.0036794949630342168 6 0.0 7 0.0 8 2.628210687881583E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0012703018324760986 28-29 0.00858548824707984 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.3803511464693054E-5 52-53 0.0 54-55 0.0 56-57 6.132491605057028E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0022339790846993457 66-67 1.7521404585877222E-4 68-69 0.006833347788492117 70-71 0.019448759090323717 72-73 8.760702292938611E-5 74-75 0.009286344430514928 76-77 3.942316031822375E-4 78-79 0.0013579088554054848 80-81 0.0012264983210114055 82-83 0.0 84-85 0.020412436342546966 86-87 0.00258440717641689 88-89 0.01962397313618249 90-91 0.0 92-93 0.014542765806278094 94-95 0.014455158783348708 96-97 0.006964758322886195 98-99 0.0032414598483872866 100-101 9.636772522232472E-4 102-103 4.3803511464693054E-5 104-105 7.008561834350889E-4 106-107 0.00157692641272895 108-109 0.0 110-111 0.0 112-113 0.001489319389799564 114-115 0.006526723208239265 116-117 0.0 118-119 0.07832067849887119 120-121 0.0020149615273758804 122-123 0.0057382600018747905 124-125 0.25270245763981425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1141461.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.67423518684842 #Duplication Level Percentage of deduplicated Percentage of total 1 82.56285980693228 39.361211961344836 2 9.549524719351522 9.105325747859741 3 2.7276906106112797 3.9012169106172103 4 1.2793057898732367 2.4395970040925428 5 0.7736107437003148 1.844065026912076 6 0.5191824257807355 1.4850975042929555 7 0.36082867577757227 1.204156180582534 8 0.27895400244933655 1.0639134975265891 9 0.2196161318775383 0.9423028009760112 >10 1.469696062423436 13.580395404168527 >50 0.1432763002829239 4.754348192168579 >100 0.10219677164417251 9.46795374195507 >500 0.007181394618531819 2.308171428892752 >1k 0.004971734735906644 4.237982881421918 >5k 0.0011048299413125877 4.304261717188625 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 9969 0.87335441158305 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 9802 0.8587240387538426 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 8875 0.7775123284983018 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 8169 0.7156617703101551 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 6879 0.602648710731247 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 5337 0.4675586813741337 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 4825 0.422703885634288 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3299 0.28901556864404476 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 2891 0.2532719032888553 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 2586 0.22655176129539248 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2438 0.21358592190184336 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2362 0.20692778815921 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 1948 0.17065848066644415 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1893 0.1658400944053279 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1865 0.1633870977633051 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1813 0.158831532570977 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1717 0.15042125836975595 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1674 0.14665415638379234 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1659 0.14534005103985156 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1606 0.14069687882459408 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1420 0.12440197255972829 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1375 0.12045965652790591 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1361 0.1192331582068945 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1301 0.11397673683113134 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1278 0.11196177530375545 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1243 0.10889552950122694 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1161 0.10171175362101727 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1155 0.10118611148344095 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.3141053439407916E-4 0.0 20-21 0.0 0.0 0.0 4.818386261116236E-4 0.0 22-23 0.0 0.0 0.0 0.00157692641272895 0.0 24-25 0.0 0.0 0.0 0.0034166738942460586 0.0 26-27 0.0 0.0 0.0 0.005125010841369088 0.0 28-29 0.0 0.0 0.0 0.010512842751526332 0.0 30-31 0.0 0.0 0.0 0.03499900566028975 0.0 32-33 0.0 0.0 0.0 0.08528543682175738 0.0 34-35 0.0 0.0 0.0 0.1463913353150042 0.0 36-37 0.0 0.0 0.0 0.22265324877503478 0.0 38-39 0.0 0.0 0.0 0.3495958249997153 0.0 40-41 0.0 0.0 0.0 0.536154980327843 0.0 42-43 0.0 0.0 0.0 0.8961322375446905 0.0 44-45 0.0 0.0 0.0 1.3977262473268905 0.0 46-47 0.0 0.0 0.0 1.9066354435236947 0.0 48-49 0.0 0.0 0.0 2.1423859422266727 0.0 50-51 0.0 0.0 0.0 2.4288171036943007 0.0 52-53 0.0 0.0 0.0 2.674598606522693 0.0 54-55 0.0 0.0 0.0 2.92265789194725 0.0 56-57 0.0 0.0 0.0 3.283642629927786 0.0 58-59 0.0 4.3803511464693054E-5 0.0 3.6855398476163446 0.0 60-61 0.0 8.760702292938611E-5 0.0 4.235405327032636 0.0 62-63 0.0 8.760702292938611E-5 0.0 4.882032763274435 0.0 64-65 0.0 8.760702292938611E-5 0.0 5.49361739034448 0.0 66-67 0.0 8.760702292938611E-5 0.0 6.019128117386402 0.0 68-69 0.0 8.760702292938611E-5 0.0 6.473107710206481 0.0 70-71 0.0 8.760702292938611E-5 0.0 6.979038267623686 0.0 72-73 0.0 8.760702292938611E-5 0.0 7.468411097707237 0.0 74-75 0.0 8.760702292938611E-5 0.0 7.973903620009795 0.0 76-77 0.0 8.760702292938611E-5 0.0 8.545933676227222 0.0 78-79 0.0 8.760702292938611E-5 0.0 9.260368948216364 0.0 80-81 0.0 8.760702292938611E-5 0.0 10.86655610660373 0.0 82-83 0.0 8.760702292938611E-5 0.0 12.037073539963258 0.0 84-85 0.0 8.760702292938611E-5 0.0 12.632144243211112 0.0 86-87 0.0 8.760702292938611E-5 0.0 13.150120766281109 0.0 88-89 0.0 8.760702292938611E-5 0.0 13.733101700364708 0.0 90-91 0.0 8.760702292938611E-5 0.0 14.434001687311262 0.0 92-93 0.0 8.760702292938611E-5 0.0 15.270867773844223 0.0 94-95 0.0 8.760702292938611E-5 0.0 16.28018828501368 0.0 96-97 0.0 8.760702292938611E-5 0.0 17.408654347367104 0.0 98-99 0.0 8.760702292938611E-5 0.0 18.53370373582628 0.0 100-101 0.0 8.760702292938611E-5 0.0 19.593442088691596 0.0 102-103 0.0 8.760702292938611E-5 0.0 20.592205953598064 0.0 104-105 0.0 8.760702292938611E-5 0.0 21.61961731500244 0.0 106-107 0.0 8.760702292938611E-5 0.0 22.60616000021026 0.0 108-109 0.0 8.760702292938611E-5 0.0 23.444121174529833 0.0 110-111 0.0 8.760702292938611E-5 0.0 24.37919473376664 0.0 112-113 0.0 8.760702292938611E-5 0.0 26.82632170525318 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACG 615 0.0 85.26624 1 GAGTAGT 1705 0.0 84.578606 1 AGTAGTA 1420 0.0 82.553986 2 AGTACGG 355 0.0 82.13493 2 GAGTCAG 4795 0.0 79.4112 1 GAGTAAG 890 0.0 77.667175 1 GAGTTAG 990 0.0 77.04498 1 GAGTCGA 325 0.0 75.17441 1 GAGTCCG 620 0.0 74.00628 1 GAGTTAC 745 0.0 73.587006 1 AGTCGAC 145 0.0 69.76556 2 GTGGGGT 5500 0.0 69.6516 7 TACGGGA 250 0.0 69.023834 4 TCGTAGG 820 0.0 68.93978 2 GAGTCGG 645 0.0 68.36621 1 TGGGGTA 5890 0.0 67.86751 8 AGTTAGT 765 0.0 66.89554 2 AGTCAGT 4130 0.0 65.2692 2 GTACGGG 630 0.0 64.2258 3 AGTAAGG 530 0.0 63.996925 2 >>END_MODULE