FastQCFastQC Report
Thu 26 May 2016
SRR935927_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935927_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3987342
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT80220.20118665517028636No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT79450.19925554416952446No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC48630.12196094541175549No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT48600.12188570732081672No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT44750.11223015231700717No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC43630.10942126358862621No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA42910.10761554940609558No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC40940.10267491476778265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114350.061.9023251
GTATCAA188800.049.5478021
TATCAAC240000.038.625832
AGTAGTA19150.037.2849962
GAGTAGT27650.037.0646631
GAGTAAG26550.035.2339941
ATCAACG278400.033.1682323
TCAACGC280550.032.9140474
GATTGTG43250.032.1833729
AGGGTAA45250.032.0758746
AACGCAG289250.031.876136
TAGTAGG26050.031.7489012
TGGTATC51700.031.6492772
CAACGCA293150.031.5348055
GAGTAGG40350.030.8623851
GAGTTAG18150.030.8587251
GTGGTAT58200.030.6108171
GTAAGGG21050.030.2434643
TAGACGG6750.029.0877444
GGGGTAA68350.028.4584379