FastQCFastQC Report
Thu 26 May 2016
SRR935927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3987342
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT86000.21568252735782384No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT81800.20514919462639522No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT52310.13119015123357866No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC48350.12125872322966025No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT45750.11473808868163304No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA43400.10884443822476227No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC43250.10846824777006839No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124600.066.008591
GTATCAA207450.051.3360521
TATCAAC272450.039.0497632
TATACGG2400.037.2517782
AGGGTAA45600.036.5330666
TAGTAGG28150.034.512482
TGGTATC53550.034.5039832
GTGGTAT56150.034.4651221
ATCAACG309750.034.3089183
TCAACGC313050.034.004374
AGTAGTA19650.033.365462
AACGCAG324150.032.9099466
CAACGCA325650.032.6756135
GATTGTG44800.032.270449
GTAGTAG33200.031.8434221
GAGTAAG26700.029.75261
GAGTAGT29950.028.9171661
TCGTAGG13400.028.467032
GATAGCG14150.026.9091599
ACGCAGA398700.026.7255067
Produced by FastQC (version 0.11.5)