FastQCFastQC Report
Thu 26 May 2016
SRR935926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3610922
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT60190.1666887293605345No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT57650.15965451483028437No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC41290.11434752675355492No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT39980.11071964445645739No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT36230.1003344852090408No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86200.059.8582081
GTATCAA144000.048.3687821
TATCAAC184200.037.6301082
ATCAACG201150.034.2802933
TCAACGC203200.033.9637344
GGGGTAA52000.033.518099
GAGTAAG23850.033.2258721
AGGGTAA37800.032.8908776
CAACGCA211050.032.8104865
GATTGTG42300.032.6259049
AACGCAG213400.032.2801176
GAGTACG12850.031.9933031
TAGTAGG24850.030.4072042
GTAGTAG27850.029.7373851
AGTACGG8750.029.2388022
TAAGGGG20300.029.0148474
GATAGCG10500.028.8932139
GAGTAGG36800.028.8194261
TGGTATC40850.028.5471782
TGGGGTA54700.028.4923928
Produced by FastQC (version 0.11.5)