FastQCFastQC Report
Thu 26 May 2016
SRR935926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3610922
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT63510.17588305701424733No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT62750.1737783314067709No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT42330.11722767758483843No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT37430.1036577361682141No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94350.067.5863341
GTATCAA163100.050.4457741
GATTGTG38550.039.200419
TATCAAC216600.037.8791962
TGGTATC40600.035.661982
ATCAACG233150.035.011473
TCAACGC235550.034.6547474
TAGTAGG24850.034.0476532
AGGGTAA35500.033.6868976
CAACGCA245000.033.3558655
GATAGCG10100.032.9873929
AACGCAG251850.032.506476
GAGTACG11300.032.2315331
GTGGTAT47200.031.7512841
GTAGTAG28950.030.3178421
GGGGTAA52950.026.629549
ACGCAGA306200.026.6193037
GGGATTG61750.026.0140577
GAGTAGT24150.025.7125871
GAGTAAG21600.025.7074361
Produced by FastQC (version 0.11.5)