FastQCFastQC Report
Thu 26 May 2016
SRR935925_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935925_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970757
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT63550.6546437470963382No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC57750.5948965601072153No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG41350.42595623827590223No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT40320.4153459619657649No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC39670.40865015652732867No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT37900.39041696325651015No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA35720.3679602619399088No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT35000.3605433697619487No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT34600.35642287410752643No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG34310.3534355147580702No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT34040.3506541801913352No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT29270.30151726951234964No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG27470.2829750390674494No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA25990.267729205146087No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC21920.22580316186234045No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA20650.21272058815954972No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT20370.20983624120145414No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC18600.19160304793063557No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT17110.1762542016179126No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG16820.17326684226845648No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC16620.17120659444124534No Hit
GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG16260.16749814835226529No Hit
GTATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCC15930.16409873943736691No Hit
ACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGC15660.1613174048706319No Hit
CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA15150.1560637729112435No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC14640.1508101409518551No Hit
CGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTGTTTTAATTAAACAGT14290.14720470725423562No Hit
GGTATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGC14120.14545349660110615No Hit
ACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAA13660.14071492659852053No Hit
CACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT13480.13886070355403052No Hit
GTATCAACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA13450.13855166637994884No Hit
GTATCAACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC12850.13237092289831545No Hit
GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG12760.13144381137607042No Hit
GTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACA12630.1301046502883832No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC12570.12948657594021987No Hit
CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT12320.12691126615620593No Hit
CTAATAGGGAACGTGAGCTGGGATTAGACCGTCGTGAGACAGGTTAGTTT12190.1255721050685187No Hit
GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG11970.12330583245858645No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC11410.11753713854239527No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG11310.11650701462878969No Hit
ACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCA11070.11403471723613634No Hit
GTGGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGG11020.11351965527933354No Hit
GACCAGGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCG10900.11228350658300688No Hit
GGTATCAACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCT10680.11001723397307463No Hit
GAGTAGGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCAC10670.10991422158171406No Hit
CTAATAGGGAACGTGAGCTGGGGTTAGACCGTCGTGAGACAGGTTAGTTT10630.10950217201627184No Hit
GTCTCACGACGGTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACA10500.1081630109285846No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA10460.10775096136314238No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCT10370.10682384984089736No Hit
AAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAG10290.1059997507100129No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTA10270.10579372592729179No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCCACTGACTCTGCGT10050.10352745331735955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGT2450.082.5433046
TAGTCAA2550.079.306319
GATAGTC2650.076.3136147
TAGATAG2800.072.2328345
CCTCTAG3000.069.493251
ATAGTCA3000.067.410368
AGTACGT954.1836756E-1156.355032
AGTAAAG2700.052.876322
GGTATCA109000.051.7510641
GAGTTAG10650.049.2185551
TCTAGAT4600.049.140493
CTATGGG1101.7826096E-1048.670253
CTAGATA4300.048.4187244
GAGTATA3950.048.2557031
TAACAAC2600.048.1107061
TCGTAGG5750.047.5886882
GTCTAGG1400.046.801571
GAGTACG6750.046.7700581
AGTCGCT1400.046.738892
AGTCGCA909.7403245E-846.266782
Produced by FastQC (version 0.11.5)