##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935925_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 970757 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.214742721401958 31.0 31.0 34.0 26.0 34.0 2 30.102503510147237 31.0 31.0 34.0 26.0 34.0 3 30.224879140711835 33.0 31.0 34.0 25.0 34.0 4 33.312021443059386 37.0 35.0 37.0 26.0 37.0 5 33.59173407969244 37.0 35.0 37.0 28.0 37.0 6 33.71575790851882 37.0 35.0 37.0 28.0 37.0 7 33.67668221810401 37.0 35.0 37.0 28.0 37.0 8 33.51596125497936 37.0 35.0 37.0 27.0 37.0 9 34.885002116904644 39.0 37.0 39.0 25.0 39.0 10-11 34.892209378866184 39.0 37.0 39.0 25.5 39.0 12-13 34.7757296625211 39.0 36.0 39.0 25.0 39.0 14-15 36.02655916980254 40.0 37.0 41.0 25.5 41.0 16-17 36.16360994564036 40.0 37.0 41.0 26.5 41.0 18-19 36.29041253372368 40.0 36.5 41.0 27.0 41.0 20-21 36.48960759489759 40.0 37.0 41.0 28.0 41.0 22-23 36.25979828113523 40.0 36.0 41.0 27.0 41.0 24-25 36.05309619194093 40.0 35.0 41.0 27.0 41.0 26-27 36.055991870262076 40.0 35.0 41.0 28.0 41.0 28-29 35.8644501147043 40.0 35.0 41.0 27.0 41.0 30-31 35.70679789071828 40.0 35.0 41.0 27.0 41.0 32-33 35.529684050694456 40.0 35.0 41.0 26.5 41.0 34-35 35.2438844118559 40.0 35.0 41.0 25.5 41.0 36-37 35.140432157584236 40.0 35.0 41.0 25.0 41.0 38-39 35.05321826162469 40.0 35.0 41.0 24.0 41.0 40-41 34.82963141136247 40.0 35.0 41.0 23.0 41.0 42-43 34.51020698279796 39.0 35.0 41.0 21.0 41.0 44-45 34.19615980106247 39.0 34.5 41.0 19.0 41.0 46-47 33.96016768357066 38.5 34.0 41.0 17.5 41.0 48-49 33.679387323501146 38.0 34.0 41.0 13.0 41.0 50-51 33.345130140704626 37.0 34.0 40.5 7.0 41.0 52-53 33.023203026092006 36.5 33.5 40.0 3.5 41.0 54-55 32.70649709453551 36.0 33.0 40.0 2.0 41.0 56-57 32.34163338507989 35.5 33.0 39.0 2.0 41.0 58-59 31.917503556502812 35.0 33.0 39.0 2.0 41.0 60-61 31.574501651803693 35.0 33.0 37.5 2.0 40.0 62-63 31.116345285174354 35.0 32.5 37.0 2.0 39.0 64-65 30.710264772749515 35.0 32.0 36.5 2.0 39.0 66-67 30.480633155362256 35.0 32.0 36.0 2.0 38.0 68-69 30.293414314807926 35.0 32.0 35.5 2.0 37.0 70-71 30.093350344112892 35.0 32.0 35.0 2.0 36.5 72-73 29.892365442639097 35.0 32.0 35.0 2.0 36.0 74-75 29.66866064318877 35.0 31.0 35.0 2.0 36.0 76-77 29.516002459935905 35.0 31.0 35.0 2.0 35.0 78-79 29.325445502839536 35.0 31.0 35.0 2.0 35.0 80-81 29.160153364848256 35.0 31.0 35.0 2.0 35.0 82-83 29.023709847057503 35.0 31.0 35.0 2.0 35.0 84-85 28.815346167990548 35.0 30.0 35.0 2.0 35.0 86-87 28.63835336752658 35.0 30.0 35.0 2.0 35.0 88-89 28.333315134477523 35.0 29.0 35.0 2.0 35.0 90-91 28.04101026312455 35.0 29.0 35.0 2.0 35.0 92-93 27.81560678934069 34.5 28.0 35.0 2.0 35.0 94-95 27.575200590879078 34.0 27.0 35.0 2.0 35.0 96-97 27.35864536645113 34.0 26.5 35.0 2.0 35.0 98-99 27.052352442475303 34.0 25.0 35.0 2.0 35.0 100-101 26.7378082259515 34.0 24.5 35.0 2.0 35.0 102-103 25.921954206871547 33.0 21.5 35.0 2.0 35.0 104-105 26.265148744742504 34.0 23.0 35.0 2.0 35.0 106-107 26.17796523743841 34.0 23.0 35.0 2.0 35.0 108-109 25.9187747294122 34.0 20.0 35.0 2.0 35.0 110-111 25.674956760548724 33.5 19.5 35.0 2.0 35.0 112-113 25.346088671006235 33.0 18.0 35.0 2.0 35.0 114-115 24.908811370919807 33.0 10.0 35.0 2.0 35.0 116-117 24.476464243883896 33.0 2.0 35.0 2.0 35.0 118-119 23.851962437561614 33.0 2.0 35.0 2.0 35.0 120-121 23.10484343661699 32.5 2.0 35.0 2.0 35.0 122-123 22.240508695791014 32.0 2.0 35.0 2.0 35.0 124-125 19.345357283027575 25.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23581806910819836 1101 2 0.3702991717238042 1101 3 0.32298188405821193 1101 4 0.21200532149378404 1101 5 0.241193438127965 1101 6 0.28677517249061424 1101 7 0.17787020240437812 1101 8 0.3094508384863417 1101 9 0.13950938080389363 1101 10-11 0.27076478489644984 1101 12-13 0.19652218557060053 1101 14-15 0.2808473854065312 1101 16-17 0.383020776278471 1101 18-19 0.43717412920839394 1101 20-21 0.4020253735918615 1101 22-23 0.36597472433780354 1101 24-25 0.41311405059239803 1101 26-27 0.5873441894007527 1101 28-29 0.530838543444851 1101 30-31 0.2212132414616761 1101 32-33 0.15975928959920083 1101 34-35 0.12669286204972252 1101 36-37 0.024131899587011674 1101 38-39 0.11740426926255054 1101 40-41 0.11200413568867162 1101 42-43 0.25091766739758725 1101 44-45 0.3583395570135153 1101 46-47 0.4194271429662422 1101 48-49 0.3424776838701149 1101 50-51 0.5581404718368361 1101 52-53 0.5622468263899734 1101 54-55 0.45024600139023363 1101 56-57 0.17303339542061735 1101 58-59 0.17302227248914193 1101 60-61 0.34235234814675053 1101 62-63 0.3676794159309047 1101 64-65 0.41646672375474125 1101 66-67 0.3322987239079431 1101 68-69 0.4087237726619293 1101 70-71 0.20493925434752214 1101 72-73 0.39075289860425855 1101 74-75 0.41358554594759056 1101 76-77 0.4168602893532558 1101 78-79 0.5081223864901006 1101 80-81 0.5289642199318862 1101 82-83 0.48794551300711575 1101 84-85 0.4035849245324563 1101 86-87 0.32490156227476064 1101 88-89 0.4112798862156559 1101 90-91 0.4156104228686175 1101 92-93 0.06358412473956321 1101 94-95 0.05006057162702504 1101 96-97 0.09807941670029052 1101 98-99 -0.02686575064584673 1101 100-101 0.08099672849353468 1101 102-103 0.3603651334078215 1101 104-105 0.13044091015339276 1101 106-107 0.04225735932065078 1101 108-109 0.016411632049706526 1101 110-111 -0.3006956223433299 1101 112-113 -0.27927639925309933 1101 114-115 -0.15305134131206444 1101 116-117 -0.22927713802777205 1101 118-119 -0.17949480498268855 1101 120-121 -0.47045218265347444 1101 122-123 -0.4708057561083656 1101 124-125 -0.29397790855582073 1102 1 0.4120476930529442 1102 2 0.42674552629019047 1102 3 0.381625015293821 1102 4 0.43078168373477155 1102 5 0.35610057550324825 1102 6 0.47895752091731936 1102 7 0.45761847562003055 1102 8 0.3089406312722147 1102 9 0.34315757727357266 1102 10-11 0.423896634474346 1102 12-13 0.45854735824904225 1102 14-15 0.4630413992208062 1102 16-17 0.41200281772130154 1102 18-19 0.4008588567601947 1102 20-21 0.449099203215809 1102 22-23 0.5373153229563741 1102 24-25 0.5778996223498822 1102 26-27 0.5795670597000679 1102 28-29 0.47823624106572993 1102 30-31 0.4722999644087196 1102 32-33 0.5631115536053386 1102 34-35 0.44857283135134196 1102 36-37 0.2886430277527836 1102 38-39 0.3182094879885753 1102 40-41 0.22731833369328314 1102 42-43 0.22068927138837324 1102 44-45 0.29462443805725513 1102 46-47 0.22218170935150283 1102 48-49 0.3260859724349672 1102 50-51 0.28150501519831295 1102 52-53 0.29032510728022487 1102 54-55 0.29147056010090466 1102 56-57 0.27137691038608125 1102 58-59 0.24500661631109466 1102 60-61 0.23515879480829582 1102 62-63 0.3222258472432564 1102 64-65 0.40571737609549174 1102 66-67 0.2839226686508418 1102 68-69 0.3094279936903277 1102 70-71 0.3514301215769926 1102 72-73 0.3173245025950493 1102 74-75 0.4262639803297823 1102 76-77 0.4210257536663775 1102 78-79 0.35707219462517514 1102 80-81 0.25072415853510677 1102 82-83 0.26215119220895744 1102 84-85 0.2852070273720564 1102 86-87 0.29737063364851224 1102 88-89 0.07211365444282336 1102 90-91 0.2755557797373811 1102 92-93 0.48456401729520593 1102 94-95 0.44451767062932745 1102 96-97 0.42867281654680056 1102 98-99 0.153118593176103 1102 100-101 0.32489388889261406 1102 102-103 0.21780151099799738 1102 104-105 0.13672947500373667 1102 106-107 0.10089404389470857 1102 108-109 0.013677940568509683 1102 110-111 0.17336362554615192 1102 112-113 0.01584593382441213 1102 114-115 -0.10445118782012486 1102 116-117 -0.06242587171925251 1102 118-119 -0.15700209584991853 1102 120-121 -0.35363928933037414 1102 122-123 -0.3402366079886434 1102 124-125 -0.30368903672158964 1103 1 0.15626318189015365 1103 2 0.1327472920245576 1103 3 0.016455568268739285 1103 4 0.19983690044115576 1103 5 0.0514490772257048 1103 6 0.06504284166354779 1103 7 0.38262208210362303 1103 8 0.22379068811039815 1103 9 0.10228832817231392 1103 10-11 0.202485085648334 1103 12-13 0.18552068181121228 1103 14-15 0.24601129518097054 1103 16-17 0.32595009958674126 1103 18-19 0.1726117231933486 1103 20-21 0.16126747885501658 1103 22-23 0.35285291982651756 1103 24-25 0.4159832235247265 1103 26-27 0.3551667457917347 1103 28-29 0.25806561111402715 1103 30-31 0.17742376777746927 1103 32-33 0.27966906403528924 1103 34-35 0.34222368911739665 1103 36-37 0.35148528304565474 1103 38-39 0.37763885572871914 1103 40-41 0.4344733086210013 1103 42-43 0.26164247942765684 1103 44-45 0.2526342938556141 1103 46-47 0.322443684319623 1103 48-49 0.14787317259191468 1103 50-51 0.13433596055864427 1103 52-53 0.19304381887117472 1103 54-55 0.22567156530001142 1103 56-57 0.22391760594693721 1103 58-59 0.22386137023350727 1103 60-61 0.19629821280840432 1103 62-63 -0.0547717118886446 1103 64-65 0.10285469367955358 1103 66-67 0.3234716562387696 1103 68-69 0.41702151702283174 1103 70-71 0.43248511900917563 1103 72-73 0.47331530461929816 1103 74-75 0.33967577151149797 1103 76-77 0.33989487581942157 1103 78-79 0.17735246167806906 1103 80-81 0.10795068609729697 1103 82-83 0.19689288142816608 1103 84-85 0.21749169144975156 1103 86-87 0.19196321641010172 1103 88-89 0.1783505629073865 1103 90-91 0.17257884392124723 1103 92-93 0.2589585608297895 1103 94-95 0.29753124831875155 1103 96-97 0.053080920459688485 1103 98-99 0.0902440237902482 1103 100-101 -0.09285289556661525 1103 102-103 -0.1892920094493249 1103 104-105 -0.22832600713983808 1103 106-107 -0.24120429481468975 1103 108-109 -0.09961844313826518 1103 110-111 0.002772046829598196 1103 112-113 -0.07154406842603223 1103 114-115 -0.21631449920680268 1103 116-117 -0.13417037111048202 1103 118-119 -0.23522412829095884 1103 120-121 -0.39527022776625387 1103 122-123 -0.5707486132512187 1103 124-125 -0.5022455777287504 1104 1 0.26719825986388557 1104 2 0.18639719079937223 1104 3 0.4181044079026712 1104 4 0.36400649537493024 1104 5 0.41008852250067207 1104 6 0.3282172854766756 1104 7 0.2928753381343867 1104 8 0.2622308825068842 1104 9 0.5461312443402093 1104 10-11 0.4773288347863982 1104 12-13 0.44024544309078095 1104 14-15 0.557644744173956 1104 16-17 0.6283146867700395 1104 18-19 0.4448081717615864 1104 20-21 0.5532955129902533 1104 22-23 0.46156738758064364 1104 24-25 0.23030231178095306 1104 26-27 0.3422190243237253 1104 28-29 0.3648394238557202 1104 30-31 0.5154069318505208 1104 32-33 0.4645487246689015 1104 34-35 0.5092325539059317 1104 36-37 0.4832793684057961 1104 38-39 0.33767807703952 1104 40-41 0.6351460285720165 1104 42-43 0.5932217026140165 1104 44-45 0.5037270845263535 1104 46-47 0.701432425431392 1104 48-49 0.6262440815223584 1104 50-51 0.6281507432968567 1104 52-53 0.5709819694567386 1104 54-55 0.6928928099008758 1104 56-57 0.7920895949804105 1104 58-59 0.857180452973374 1104 60-61 0.6844380414702975 1104 62-63 0.69435146288615 1104 64-65 0.6403593136300181 1104 66-67 0.6091042998433771 1104 68-69 0.7750257535863625 1104 70-71 0.7942493057048203 1104 72-73 0.7544203967700689 1104 74-75 0.8324556853459733 1104 76-77 0.8712106928748966 1104 78-79 0.933370809087311 1104 80-81 0.9472453644659993 1104 82-83 1.046705601048199 1104 84-85 1.0049243229183702 1104 86-87 1.0253940054148956 1104 88-89 0.9781136352985627 1104 90-91 0.9585926679163066 1104 92-93 0.9899764945121206 1104 94-95 0.9875731174817552 1104 96-97 0.8388183748807769 1104 98-99 0.810413486331413 1104 100-101 0.7352579170481945 1104 102-103 0.8573068795560204 1104 104-105 0.848763727468139 1104 106-107 0.7445872199222627 1104 108-109 0.885595784654253 1104 110-111 0.8722927855803668 1104 112-113 0.651488384312561 1104 114-115 0.6527784459219745 1104 116-117 0.41206636894214554 1104 118-119 0.5064685112315459 1104 120-121 0.7921679200611251 1104 122-123 0.6825791301718986 1104 124-125 0.49800624404872806 1105 1 0.12604538687182298 1105 2 0.3112061780932329 1105 3 0.23936688051963984 1105 4 0.2643320023147311 1105 5 0.34070086912583974 1105 6 0.28397658791877944 1105 7 0.3264561896655209 1105 8 0.43935826948421663 1105 9 0.30071645794472346 1105 10-11 0.36682055276622805 1105 12-13 0.49954752173479733 1105 14-15 0.5159887866804169 1105 16-17 0.5708449801001265 1105 18-19 0.6650051270852444 1105 20-21 0.6502923233441678 1105 22-23 0.5822328984354641 1105 24-25 0.6329212692760464 1105 26-27 0.6583522573413063 1105 28-29 0.585377255405227 1105 30-31 0.7187971055805704 1105 32-33 0.767139332062051 1105 34-35 0.7442169950999755 1105 36-37 0.757761057407258 1105 38-39 0.7034291807982882 1105 40-41 0.8029329396518747 1105 42-43 0.7778818570649548 1105 44-45 0.7482781274310639 1105 46-47 0.7383890679485461 1105 48-49 0.6681207128863917 1105 50-51 0.5537696296570829 1105 52-53 0.6524846183220347 1105 54-55 0.6261041754879031 1105 56-57 0.5276927018608149 1105 58-59 0.5507771203306149 1105 60-61 0.5490351506945181 1105 62-63 0.7117374484857528 1105 64-65 0.6622031710300398 1105 66-67 0.6262732462009346 1105 68-69 0.6831254711757282 1105 70-71 0.6895037271005293 1105 72-73 0.8037456799206062 1105 74-75 0.6881219932228895 1105 76-77 0.6633143587092363 1105 78-79 0.7239564983089224 1105 80-81 0.6488065412340802 1105 82-83 0.6999695752859552 1105 84-85 0.8039285904914095 1105 86-87 0.817039708063863 1105 88-89 0.9219199428327833 1105 90-91 0.9089329989478827 1105 92-93 0.8137276491557017 1105 94-95 0.6854389155760678 1105 96-97 0.7119132454334824 1105 98-99 0.9574736690286088 1105 100-101 0.8942012578636707 1105 102-103 0.7641100732521231 1105 104-105 0.7829348945836827 1105 106-107 0.7824099424770523 1105 108-109 0.7952495655245819 1105 110-111 0.6880539884139196 1105 112-113 0.645615603577756 1105 114-115 0.731553046515252 1105 116-117 0.5125991535504291 1105 118-119 0.429682831252272 1105 120-121 0.20369587113279408 1105 122-123 0.16502127856962545 1105 124-125 0.11395868026230005 1106 1 -0.0573972067050974 1106 2 0.018081915712489405 1106 3 0.16121102125764608 1106 4 -0.4864580448428484 1106 5 -0.07796356328646681 1106 6 -0.10078013161122357 1106 7 -0.01728114130142444 1106 8 -0.001229783861610656 1106 9 0.2722652506765897 1106 10-11 0.2721865670746695 1106 12-13 0.27407126619295497 1106 14-15 0.44378543349705524 1106 16-17 0.4125800407763549 1106 18-19 0.3149291504247955 1106 20-21 0.32096002705721105 1106 22-23 0.37085336648772227 1106 24-25 0.31309481438305653 1106 26-27 0.22853457129609467 1106 28-29 0.2133771573062475 1106 30-31 0.2690265370769538 1106 32-33 0.2916433065723254 1106 34-35 0.2689451958545348 1106 36-37 0.33488437483453737 1106 38-39 0.3160107756274755 1106 40-41 0.2780488315868368 1106 42-43 0.21717735622360834 1106 44-45 0.2192798681874848 1106 46-47 0.3142288402788296 1106 48-49 0.4192533557230149 1106 50-51 0.49333990606597666 1106 52-53 0.3973727104069482 1106 54-55 0.3970443040776459 1106 56-57 0.40256720022966874 1106 58-59 0.4301741819658105 1106 60-61 0.545010339660184 1106 62-63 0.49328903403557334 1106 64-65 0.4773131169654903 1106 66-67 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-1.3771671464135977 2216 96-97 -1.3582493766091481 2216 98-99 -1.4174530863686634 2216 100-101 -1.3052732834538965 2216 102-103 -1.3254671246566971 2216 104-105 -1.8241277875765967 2216 106-107 -1.7419548270592813 2216 108-109 -1.8963697299574598 2216 110-111 -2.163542934171293 2216 112-113 -2.2151454554060273 2216 114-115 -2.0117084500336944 2216 116-117 -2.0200432073228747 2216 118-119 -1.9805915791762416 2216 120-121 -1.8024966270979164 2216 122-123 -1.5367837728347666 2216 124-125 -1.1785231893204546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33818.0 3 5157.0 4 2680.0 5 4404.0 6 8147.0 7 9592.0 8 11454.0 9 8673.0 10 7511.0 11 6570.0 12 5219.0 13 4185.0 14 3706.0 15 3357.0 16 3297.0 17 3318.0 18 3538.0 19 3899.0 20 4502.0 21 5339.0 22 6803.0 23 8685.0 24 10624.0 25 12607.0 26 14028.0 27 15024.0 28 17094.0 29 20196.0 30 24441.0 31 32156.0 32 43046.0 33 57630.0 34 85430.0 35 123295.0 36 203937.0 37 147147.0 38 10214.0 39 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 63.34953484701074 15.370115687267797 8.72911144791184 12.551238017809629 2 16.0554589364017 29.997259404569583 37.24778279647885 16.699498862549866 3 35.39427243338134 22.65085909378432 20.520689747536245 21.434178725298093 4 14.110831447060557 20.216585669409618 39.20250069801537 26.470082185514453 5 16.802837490226295 34.67746006302558 25.932892902874887 22.58680954387324 6 30.451286985311466 41.28839658122476 11.001414360133381 17.2589020733304 7 36.08965008570675 35.054988297732066 12.144502406381855 16.710859210179326 8 40.099283751891576 26.11632413188525 11.631177034734892 22.153215081488288 9 45.54455955506888 14.62054870580382 12.66846388952127 27.166427849606027 10-11 36.16363312342842 28.243319388889287 14.205305756229416 21.387741731452877 12-13 32.053129670968126 29.074320349994903 18.402391123628263 20.47015885540872 14-15 31.442008659221614 25.938983700349315 20.355506063824418 22.26350157660465 16-17 28.65222707639502 31.029701562800987 19.723885586197163 20.594185774606828 18-19 27.18785442700902 25.317097893705633 23.03846379681012 24.456583882475222 20-21 32.58184076962618 24.877131970204697 18.99646358460459 23.544563675564532 22-23 33.76292934277064 22.931794465556262 19.330275238808476 23.97500095286462 24-25 26.116371038272195 25.567005955146342 26.186161933418973 22.13046107316249 26-27 23.22517939811238 26.740774620755587 24.056498816374212 25.977547164757826 28-29 23.897133438071442 25.419321606098848 22.956344811600175 27.72720014422953 30-31 23.308459274566136 27.279792986298325 23.281882077595114 26.129865661540425 32-33 26.077998922490387 26.720281182623456 20.801395199828587 26.40032469505757 34-35 25.564894201123455 23.5310690522963 23.168825978076903 27.735210768503343 36-37 24.608475653536363 26.947114468399402 23.690532234122443 24.75387764394179 38-39 23.115723090330537 27.957047953298304 22.962183121007627 25.965045835363536 40-41 22.921338707833165 28.862063317596476 23.18355675004146 25.0330412245289 42-43 22.673233363241263 26.218765355284585 21.439093408546114 29.668907872928035 44-45 23.04232676148614 27.328878390781625 21.738344405448533 27.890450442283704 46-47 24.725188692947874 25.49592740510756 22.334734645230476 27.44414925671409 48-49 20.142012882729663 25.163506418187044 25.290005634777806 29.404475064305487 50-51 22.552914890132133 27.863924751508357 24.556969457856084 25.02619090050342 52-53 23.81224137451494 25.953920497096593 24.869921102809457 25.363917025579006 54-55 21.253362066923028 26.32100515370994 24.72008957957553 27.705543199791506 56-57 21.1933229049865 27.148399976719066 23.2563637552968 28.40191336299764 58-59 21.617150327012837 25.83658938333692 26.649666188345794 25.896594101304444 60-61 21.82633947150468 28.065136862404145 25.191088182818337 24.917435483272833 62-63 22.85242341801295 26.061259408894294 24.52086361468421 26.565453558408542 64-65 24.521564185728124 24.029260106835856 27.292281209776238 24.156894497659778 66-67 24.00134534321704 25.739423706884118 24.97213511943269 25.28709583046615 68-69 22.12927631307845 26.890859754238193 24.53656963663186 26.443294296051494 70-71 22.634368137090505 27.79118054793547 23.788586197499306 25.78586511747472 72-73 22.917665673797366 27.007727486478316 24.107975695218826 25.96663114450549 74-75 23.187806329149787 27.11953372292778 25.196527132102393 24.49613281582004 76-77 24.022872942063593 26.17125450938137 23.72892617491146 26.07694637364358 78-79 23.97081834916822 26.814453199443317 24.021140420727544 25.193588030660923 80-81 22.182876786061037 24.984020029946027 26.500533352425172 26.33256983156777 82-83 23.05186728082686 24.429452700033426 25.31654436514203 27.202135653997683 84-85 23.2253497835538 25.022293727001145 26.345698817543813 25.40665767190125 86-87 24.20977322314867 24.095478266960875 24.86360836233201 26.831140147558447 88-89 22.76085898198734 25.898610864262405 24.771177687280897 26.569352466469358 90-91 22.38143384199214 26.781202887853844 24.40254008347107 26.434823186682948 92-93 22.88792741520467 25.749304694490565 24.429164350698755 26.933603539606015 94-95 22.18371294562482 25.855962827787465 25.05451426540321 26.905809961184502 96-97 23.445915318842072 24.86463376944473 25.114968579375706 26.57448233233749 98-99 23.751945698653902 25.398333832956816 25.15243666886089 25.697283799528396 100-101 23.9007199595364 26.581897071230564 23.866983123340844 25.650399845892192 102-103 23.136995149146493 28.122022298062234 24.297996305975648 24.44298624681563 104-105 21.977446350870252 28.63887996110232 24.177750857067508 25.205922830959913 106-107 21.53109209936698 29.514136050147048 23.162622521877527 25.79214932860844 108-109 22.32989306283653 30.442633944437176 23.399985784289992 23.827487208436303 110-111 22.557292916764958 30.92004487219768 23.047425874858487 23.475236336178877 112-113 22.116753507096604 31.94749192888768 23.236922943797865 22.698831620217852 114-115 22.827638391038224 32.11428241769732 23.22400163593199 21.834077555332463 116-117 23.760941203617385 32.762163960702836 21.80452986689769 21.672364968782095 118-119 23.264158247223143 32.94652259500127 21.756209753032522 22.03310940474307 120-121 23.092030543787583 32.82066470080738 21.652454963365482 22.434849792039557 122-123 23.524379018678758 33.18059156187561 21.848992713001884 21.446036706443742 124-125 23.446595475090692 34.96054722715317 20.659652840382787 20.933204457373346 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 51.0 1 72.5 2 112.0 3 141.0 4 151.0 5 149.0 6 129.5 7 120.0 8 141.5 9 169.0 10 182.0 11 177.5 12 211.5 13 262.0 14 316.5 15 388.5 16 481.5 17 618.0 18 791.5 19 995.0 20 1207.5 21 1460.5 22 1778.5 23 2149.0 24 2537.0 25 2946.5 26 3431.0 27 4011.5 28 4549.0 29 4881.0 30 5207.5 31 5506.5 32 5721.5 33 5941.0 34 6074.0 35 6128.0 36 6183.0 37 6120.5 38 5872.5 39 5676.5 40 5405.0 41 5168.5 42 5163.5 43 5438.0 44 5926.5 45 8554.5 46 16919.5 47 29857.5 48 47098.0 49 72943.0 50 94664.0 51 95835.0 52 82012.0 53 66051.5 54 53875.0 55 46956.5 56 42709.5 57 37547.0 58 31354.5 59 24295.5 60 19066.0 61 15041.5 62 10807.0 63 7947.0 64 6399.0 65 6326.0 66 6341.5 67 5573.0 68 4914.5 69 3852.0 70 2777.0 71 2219.5 72 1800.5 73 1532.0 74 1355.5 75 1067.0 76 692.5 77 572.5 78 505.5 79 347.5 80 245.5 81 199.0 82 153.5 83 79.5 84 44.0 85 28.5 86 22.0 87 15.0 88 9.0 89 6.5 90 5.0 91 3.5 92 2.0 93 1.5 94 3.0 95 4.0 96 2.0 97 1.0 98 0.5 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15513666138899848 2 0.016997044574491867 3 0.00855002848292621 4 0.0152458339213624 5 0.00412049565442227 6 0.0 7 5.150619568027837E-4 8 4.1204956544222706E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0011331363049661243 28-29 0.008447016091565654 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 5.665681524830621E-4 58-59 0.0 60-61 1.0301239136055676E-4 62-63 0.0 64-65 0.0015966920660886298 66-67 1.0301239136055676E-4 68-69 0.006283755872993963 70-71 0.019211810988743836 72-73 1.5451858704083513E-4 74-75 0.00921960902676983 76-77 3.0903717408167026E-4 78-79 0.0010301239136055675 80-81 0.0010816301092858459 82-83 5.150619568027838E-5 84-85 0.019829885336907178 86-87 0.0023692850012928053 88-89 0.019520848162825507 90-91 1.5451858704083513E-4 92-93 0.014473240986158223 94-95 0.014370228594797666 96-97 0.005871706307551736 98-99 0.0026783221753744757 100-101 8.240991308844541E-4 102-103 0.0 104-105 5.150619568027837E-4 106-107 0.001236148696326681 108-109 0.0 110-111 0.0 112-113 0.0011331363049661243 114-115 0.005820200111871458 116-117 0.0 118-119 0.07525055188888671 120-121 0.0020087416315308568 122-123 0.0057171877205109 124-125 0.2455815410035673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 970757.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.58303190814653 #Duplication Level Percentage of deduplicated Percentage of total 1 80.11862029048473 32.51456523685418 2 9.52714864484315 7.732811548946492 3 3.414998273709857 4.157729517246973 4 1.8023561238670536 2.9258030433895974 5 1.0940160999677593 2.21992451465088 6 0.7286710749230043 1.7743008890486232 7 0.5063352587332508 1.4384033972973804 8 0.39310164042516876 1.2762605133215497 9 0.2849226848893979 1.0406723770999096 >10 1.7799267497421927 13.777149318930471 >50 0.18915090010132332 5.3252602700189176 >100 0.1328614879440261 10.67158090012974 >500 0.014706042558247215 4.314463697021183 >1k 0.012677622895040703 9.582906571582575 >5k 5.071049158016281E-4 1.248168204461517 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 6355 0.6546437470963382 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 5775 0.5948965601072153 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 4135 0.42595623827590223 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 4032 0.4153459619657649 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 3967 0.40865015652732867 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 3790 0.39041696325651015 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 3572 0.3679602619399088 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 3500 0.3605433697619487 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 3460 0.35642287410752643 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3431 0.3534355147580702 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 3404 0.3506541801913352 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 2927 0.30151726951234964 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 2747 0.2829750390674494 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2599 0.267729205146087 No Hit ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC 2192 0.22580316186234045 No Hit TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA 2065 0.21272058815954972 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 2037 0.20983624120145414 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 1860 0.19160304793063557 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1711 0.1762542016179126 No Hit GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG 1682 0.17326684226845648 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 1662 0.17120659444124534 No Hit GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1626 0.16749814835226529 No Hit GTATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCC 1593 0.16409873943736691 No Hit ACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGC 1566 0.1613174048706319 No Hit CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA 1515 0.1560637729112435 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 1464 0.1508101409518551 No Hit CGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTGTTTTAATTAAACAGT 1429 0.14720470725423562 No Hit GGTATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGC 1412 0.14545349660110615 No Hit ACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAA 1366 0.14071492659852053 No Hit CACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT 1348 0.13886070355403052 No Hit GTATCAACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA 1345 0.13855166637994884 No Hit GTATCAACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC 1285 0.13237092289831545 No Hit GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1276 0.13144381137607042 No Hit GTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1263 0.1301046502883832 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1257 0.12948657594021987 No Hit CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT 1232 0.12691126615620593 No Hit CTAATAGGGAACGTGAGCTGGGATTAGACCGTCGTGAGACAGGTTAGTTT 1219 0.1255721050685187 No Hit GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG 1197 0.12330583245858645 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1141 0.11753713854239527 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1131 0.11650701462878969 No Hit ACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCA 1107 0.11403471723613634 No Hit GTGGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGG 1102 0.11351965527933354 No Hit GACCAGGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCG 1090 0.11228350658300688 No Hit GGTATCAACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCT 1068 0.11001723397307463 No Hit GAGTAGGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCAC 1067 0.10991422158171406 No Hit CTAATAGGGAACGTGAGCTGGGGTTAGACCGTCGTGAGACAGGTTAGTTT 1063 0.10950217201627184 No Hit GTCTCACGACGGTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1050 0.1081630109285846 No Hit CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 1046 0.10775096136314238 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCT 1037 0.10682384984089736 No Hit AAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAG 1029 0.1059997507100129 No Hit CCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTA 1027 0.10579372592729179 No Hit GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCCACTGACTCTGCGT 1005 0.10352745331735955 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.150619568027838E-5 0.0 18-19 0.0 0.0 0.0 3.0903717408167026E-4 0.0 20-21 5.150619568027838E-5 0.0 0.0 0.0014936796747280733 0.0 22-23 1.0301239136055676E-4 0.0 0.0 0.006798817829796746 0.0 24-25 1.0301239136055676E-4 0.0 0.0 0.014473240986158225 0.0 26-27 1.0301239136055676E-4 0.0 0.0 0.030388655451364245 0.0 28-29 1.5451858704083513E-4 0.0 0.0 0.08524275385086072 0.0 30-31 2.0602478272111353E-4 0.0 0.0 0.24686919589557427 0.0 32-33 2.0602478272111353E-4 0.0 0.0 0.49492303429179496 0.0 34-35 2.0602478272111353E-4 0.0 0.0 0.7884053372780211 0.0 36-37 3.6054336976194866E-4 0.0 0.0 1.2676704880830116 0.0 38-39 4.1204956544222706E-4 0.0 0.0 2.065758990148925 0.0 40-41 4.6355576112250545E-4 0.0 0.0 3.0216624757792117 0.0 42-43 6.180743481633405E-4 0.0 0.0 4.055494835473759 0.0 44-45 6.180743481633405E-4 0.0 0.0 5.341862072588711 0.0 46-47 6.180743481633405E-4 0.0 0.0 6.771004484129396 0.0 48-49 6.180743481633405E-4 0.0 0.0 7.8498017526528265 0.0 50-51 6.180743481633405E-4 0.0 0.0 9.19158965631976 0.0 52-53 6.180743481633405E-4 0.0 0.0 10.1580519120645 0.0 54-55 6.180743481633405E-4 0.0 0.0 11.093249907031318 0.0 56-57 6.180743481633405E-4 0.0 0.0 12.399395523287495 0.0 58-59 6.695805438436189E-4 0.0 0.0 13.754472025439942 0.0 60-61 7.210867395238973E-4 0.0 0.0 15.331282699995981 0.0 62-63 7.210867395238973E-4 0.0 0.0 16.872090543771513 0.0 64-65 7.210867395238973E-4 0.0 0.0 18.316015233472434 0.0 66-67 7.210867395238973E-4 0.0 0.0 19.651931430831816 0.0 68-69 7.210867395238973E-4 0.0 0.0 21.004432623200245 0.0 70-71 7.210867395238973E-4 0.0 0.0 22.67055504106589 0.0 72-73 7.210867395238973E-4 0.0 0.0 24.297429737823162 0.0 74-75 7.210867395238973E-4 0.0 0.0 26.016552031043815 0.0 76-77 7.210867395238973E-4 0.0 0.0 27.951639802751874 0.0 78-79 7.210867395238973E-4 0.0 0.0 30.039391938456276 0.0 80-81 7.210867395238973E-4 0.0 0.0 32.25487943944777 0.0 82-83 7.210867395238973E-4 0.0 0.0 34.06959723185102 0.0 84-85 7.210867395238973E-4 1.0301239136055676E-4 0.0 35.597940576271924 0.0 86-87 7.210867395238973E-4 1.0301239136055676E-4 0.0 37.082452148168905 0.0 88-89 7.210867395238973E-4 1.0301239136055676E-4 0.0 38.70180694035686 0.0 90-91 7.210867395238973E-4 1.0301239136055676E-4 0.0 40.337128653205696 0.0 92-93 7.210867395238973E-4 1.0301239136055676E-4 0.0 42.26629321241052 0.0 94-95 7.210867395238973E-4 1.0301239136055676E-4 0.0 44.49913830134627 0.0 96-97 7.210867395238973E-4 1.0301239136055676E-4 0.0 46.79492396140331 0.0 98-99 7.210867395238973E-4 1.0301239136055676E-4 0.0 48.8563564311151 0.0 100-101 7.210867395238973E-4 1.0301239136055676E-4 0.0 50.63022981034389 0.0 102-103 7.210867395238973E-4 1.0301239136055676E-4 0.0 52.32761648898746 0.0 104-105 7.210867395238973E-4 1.0301239136055676E-4 0.0 54.013568792190014 0.0 106-107 7.210867395238973E-4 1.5451858704083513E-4 0.0 55.706886481374845 0.0 108-109 7.210867395238973E-4 2.0602478272111353E-4 0.0 57.285911922345136 0.0 110-111 7.210867395238973E-4 2.0602478272111353E-4 0.0 58.73436915726593 0.0 112-113 7.210867395238973E-4 2.0602478272111353E-4 0.0 60.36639447359123 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATAGT 245 0.0 82.543304 6 TAGTCAA 255 0.0 79.30631 9 GATAGTC 265 0.0 76.313614 7 TAGATAG 280 0.0 72.232834 5 CCTCTAG 300 0.0 69.49325 1 ATAGTCA 300 0.0 67.41036 8 AGTACGT 95 4.1836756E-11 56.35503 2 AGTAAAG 270 0.0 52.87632 2 GGTATCA 10900 0.0 51.751064 1 GAGTTAG 1065 0.0 49.218555 1 TCTAGAT 460 0.0 49.14049 3 CTATGGG 110 1.7826096E-10 48.67025 3 CTAGATA 430 0.0 48.418724 4 GAGTATA 395 0.0 48.255703 1 TAACAAC 260 0.0 48.110706 1 TCGTAGG 575 0.0 47.588688 2 GTCTAGG 140 0.0 46.80157 1 GAGTACG 675 0.0 46.770058 1 AGTCGCT 140 0.0 46.73889 2 AGTCGCA 90 9.7403245E-8 46.26678 2 >>END_MODULE