FastQCFastQC Report
Thu 26 May 2016
SRR935925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970757
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT62880.6477419168751809No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC47840.49281128026890353No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT44740.46087743894713096No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT38330.39484649608501404No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC37660.3879446658638568No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA37080.3819699471649445No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG36920.38032174890317555No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT36670.3777464391191616No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT36070.3715656956375282No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT35830.36909339824487486No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG34610.35652588649888695No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG30590.3151149051719431No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT27930.287713609070035No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA21690.22343387686104763No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA21430.22075555468567312No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC21410.22054952990295204No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT19820.2041705596766235No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT18190.18737953988485276No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG17750.18284699466498824No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC16670.17172165639804812No Hit
CGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTGTTTTAATTAAACAGT16280.16770417313498642No Hit
CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA16240.16729212356954418No Hit
ACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGC16090.16574693769913582No Hit
GTATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCC15960.1644077766114486No Hit
CACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT15890.1636866898719247No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC15890.1636866898719247No Hit
GGTATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGC15250.15709389682484906No Hit
GTATCAACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC15240.15699088443348852No Hit
ACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAA14520.14957399225552842No Hit
CTAATAGGGAACGTGAGCTGGGATTAGACCGTCGTGAGACAGGTTAGTTT14150.14576253377518783No Hit
CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT13190.13587334420457436No Hit
GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG13080.13474020789960825No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC13000.13391610876872378No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG12890.13278297246375767No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTA12730.13113477420198877No Hit
GTATCAACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA12680.13061971224518598No Hit
GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG12670.1305166998538254No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC12550.12928055115749873No Hit
CTAATAGGGAACGTGAGCTGGGGTTAGACCGTCGTGAGACAGGTTAGTTT12420.12794139006981148No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT12250.12619017941668204No Hit
GTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACA12190.1255721050685187No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTG11810.12165763419681754No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA11320.11661002702015025No Hit
GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG11190.11527086593246302No Hit
GACCAGGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCG11170.1150648411497419No Hit
AAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAG10970.11300459332253077No Hit
GGTATCAACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCG10960.11290158093117023No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG10940.1126955561484491No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCCACTGACTCTGCGT10910.11238651897436742No Hit
GGTATCAACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCT10640.10960518440763241No Hit
ACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCA10610.10929614723355072No Hit
GACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCC10560.10878108527674794No Hit
ACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTAC10170.10476360201368623No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCT10140.10445456483960457No Hit
AAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACGTTCCCTATTAG10050.10352745331735955No Hit
TTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA10020.10321841614327788No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA9990.1029093789691962No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG9940.10239431701239343No Hit
GTCTCACGACGGTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACA9870.10167323027286952No Hit
TATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCG9830.10126118070742729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTC2400.064.453317
AGATAGT2450.063.137946
TAGGACG401.760372E-459.578224
TCATAGG1450.057.5237962
TAGATAG2700.057.2947855
GGTATCA116650.056.9062041
TAGTCAA2850.056.364039
TATAAGG752.2882887E-855.6063352
GTACTAG1103.6379788E-1254.2683831
AGTATAA551.3003993E-554.1620182
CATAGGG1550.053.8125843
TCGGGTA453.1710978E-452.887495
CCTCTAG3050.050.8877261
GTACAGG3650.050.700051
AGTAGAG3650.050.6006772
GAGTAAG11350.050.491111
GTCGAAG602.1510521E-549.7460171
GAGTAGA7000.049.461751
GTTGGAG1450.049.306113
GTCTTAG856.042319E-849.1607671
Produced by FastQC (version 0.11.5)