##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935925_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 970757 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45557745141163 33.0 31.0 34.0 31.0 34.0 2 32.51208901918812 34.0 31.0 34.0 31.0 34.0 3 32.60369072795767 34.0 31.0 34.0 31.0 34.0 4 35.9665652681361 37.0 35.0 37.0 35.0 37.0 5 36.01069989709062 37.0 35.0 37.0 35.0 37.0 6 36.119834314869735 37.0 36.0 37.0 35.0 37.0 7 36.13150252843914 37.0 36.0 37.0 35.0 37.0 8 36.140567618878876 37.0 37.0 37.0 35.0 37.0 9 37.883447659919014 39.0 38.0 39.0 35.0 39.0 10-11 37.746602908863906 39.0 38.0 39.0 35.0 39.0 12-13 37.66905209027594 39.0 37.5 39.0 35.0 39.0 14-15 38.98219894371094 40.0 38.0 41.0 35.0 41.0 16-17 38.96971590212587 40.0 38.0 41.0 35.0 41.0 18-19 39.00143238730187 41.0 38.5 41.0 35.0 41.0 20-21 39.0589462656463 41.0 39.0 41.0 35.0 41.0 22-23 38.79878177545977 40.5 38.0 41.0 35.0 41.0 24-25 38.47398010006624 40.0 38.0 41.0 34.0 41.0 26-27 38.38098051314593 40.0 37.5 41.0 34.0 41.0 28-29 38.197979514955854 40.0 37.0 41.0 34.0 41.0 30-31 37.976517295265445 40.0 36.5 41.0 33.5 41.0 32-33 37.66475132293664 40.0 35.5 41.0 33.0 41.0 34-35 37.26659452365525 40.0 35.0 41.0 33.0 41.0 36-37 36.962832099073196 40.0 35.0 41.0 32.5 41.0 38-39 36.40020262537381 40.0 35.0 41.0 31.0 41.0 40-41 35.98280980719171 40.0 35.0 41.0 30.0 41.0 42-43 35.68261521678443 39.0 35.0 41.0 28.5 41.0 44-45 35.34439669247814 39.0 35.0 41.0 27.5 41.0 46-47 34.96069356182855 38.5 35.0 41.0 26.0 41.0 48-49 34.45329881731473 38.0 35.0 41.0 23.0 41.0 50-51 33.93051299140774 37.0 34.5 41.0 20.0 41.0 52-53 33.535176156339844 36.5 34.0 40.0 17.5 41.0 54-55 33.221811946759075 36.0 34.0 40.0 14.5 41.0 56-57 32.898824834639356 35.5 34.0 39.0 13.0 41.0 58-59 32.541604129560746 35.0 34.0 39.0 11.0 41.0 60-61 32.22949358078283 35.0 33.5 38.0 9.0 40.0 62-63 31.817994616572427 35.0 33.0 37.0 6.5 39.5 64-65 31.436260052721742 35.0 33.0 36.5 6.0 39.0 66-67 31.120219581213426 35.0 33.0 36.0 2.0 38.0 68-69 30.787848555302716 35.0 33.0 35.0 2.0 37.0 70-71 30.54977095194781 35.0 32.5 35.0 2.0 36.5 72-73 30.329708155594034 35.0 32.0 35.0 2.0 36.0 74-75 30.12868101903978 35.0 32.0 35.0 2.0 36.0 76-77 30.01744720872474 35.0 32.0 35.0 2.0 35.0 78-79 30.10354032986628 35.0 32.0 35.0 2.0 35.0 80-81 30.08170788364132 35.0 32.0 35.0 2.0 35.0 82-83 30.029882349547826 35.0 32.0 35.0 2.0 35.0 84-85 29.927574562944177 35.0 32.0 35.0 2.0 35.0 86-87 29.872827597431694 35.0 32.0 35.0 2.0 35.0 88-89 29.73717882024029 35.0 32.0 35.0 2.0 35.0 90-91 29.63907651451393 35.0 31.0 35.0 2.0 35.0 92-93 29.629530356206548 35.0 31.0 35.0 2.0 35.0 94-95 29.571930462515333 35.0 31.0 35.0 2.0 35.0 96-97 29.554179882298044 35.0 31.0 35.0 2.0 35.0 98-99 29.463365188198487 35.0 31.0 35.0 2.0 35.0 100-101 29.362710750476175 35.0 31.0 35.0 2.0 35.0 102-103 28.6105642297712 33.5 29.5 35.0 2.0 35.0 104-105 29.189744704390492 35.0 31.0 35.0 2.0 35.0 106-107 29.23461175144758 35.0 31.0 35.0 2.0 35.0 108-109 29.11126471403245 35.0 31.0 35.0 2.0 35.0 110-111 28.94334215462778 35.0 31.0 35.0 2.0 35.0 112-113 28.728983669445597 35.0 31.0 35.0 2.0 35.0 114-115 28.344685642235905 35.0 30.0 35.0 2.0 35.0 116-117 27.975610786221473 35.0 29.0 35.0 2.0 35.0 118-119 27.57710271468555 35.0 29.0 35.0 2.0 35.0 120-121 27.038987614820186 34.5 27.0 35.0 2.0 35.0 122-123 26.416825219905704 34.0 24.5 35.0 2.0 35.0 124-125 23.870552568768495 30.5 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07019910804854845 1101 2 0.13438117901149838 1101 3 0.1422247637395131 1101 4 0.17695077444052743 1101 5 0.22624825088856682 1101 6 0.16727972125446655 1101 7 0.192919852050089 1101 8 0.19345110802318288 1101 9 0.2911581566416004 1101 10-11 0.1645246820967614 1101 12-13 0.20734038058203197 1101 14-15 0.3340808724610085 1101 16-17 0.2049944518326896 1101 18-19 0.3684327204131179 1101 20-21 0.2543906330280805 1101 22-23 0.294962051247758 1101 24-25 0.28203440542995395 1101 26-27 0.3588956946677442 1101 28-29 0.28923533147550273 1101 30-31 0.189120855570998 1101 32-33 0.25898491088647546 1101 34-35 0.3759828018201645 1101 36-37 0.4969013629804664 1101 38-39 0.38278163701765067 1101 40-41 0.38621474784147125 1101 42-43 0.22675619479255005 1101 44-45 0.24706646068501925 1101 46-47 0.2819950944167857 1101 48-49 0.3969206147843636 1101 50-51 0.33561652012984666 1101 52-53 0.36432793491223237 1101 54-55 0.3312845821853685 1101 56-57 0.2827818772045063 1101 58-59 0.25471035281216103 1101 60-61 0.19654120587324542 1101 62-63 0.25758512303048064 1101 64-65 0.2573969539242036 1101 66-67 0.11760422669797066 1101 68-69 0.2004802789332878 1101 70-71 0.16433086919778717 1101 72-73 0.16395289048672623 1101 74-75 0.1366735422019012 1101 76-77 0.08222519110653081 1101 78-79 0.028092353757671162 1101 80-81 0.07334254518107386 1101 82-83 0.051405739407918816 1101 84-85 -0.04527703908498992 1101 86-87 -0.07488006211613296 1101 88-89 -0.20485556829059348 1101 90-91 -0.07891759104660068 1101 92-93 -0.02576402843238057 1101 94-95 -0.08636966614270136 1101 96-97 -0.032218644678447816 1101 98-99 -0.007849153834204259 1101 100-101 -0.1352375931477141 1101 102-103 -0.15225420454704874 1101 104-105 0.018727943899847332 1101 106-107 -0.12510963672172437 1101 108-109 -0.18069708210211033 1101 110-111 -0.08664210143073703 1101 112-113 -0.2194821294649607 1101 114-115 -0.16096586506623822 1101 116-117 -0.15165529857205584 1101 118-119 -0.3875060648718822 1101 120-121 -0.24158842547904058 1101 122-123 -0.34215227464157394 1101 124-125 -0.417936144168376 1102 1 0.08360263836320314 1102 2 0.0736045097866338 1102 3 0.05311471001733281 1102 4 0.14931178748734197 1102 5 0.2265282201901826 1102 6 0.21039929147703162 1102 7 0.18816958343919055 1102 8 0.15873737049439995 1102 9 0.2039231604789009 1102 10-11 0.26269014640988786 1102 12-13 0.30458642816453363 1102 14-15 0.41612538512409003 1102 16-17 0.39785922543207164 1102 18-19 0.26376226761189514 1102 20-21 0.2876593974179542 1102 22-23 0.19524816022703106 1102 24-25 0.29816671548367424 1102 26-27 0.3107701382594499 1102 28-29 0.3204571273312169 1102 30-31 0.2573873175817383 1102 32-33 0.31634085869922046 1102 34-35 0.24453752169737442 1102 36-37 0.3771355916834551 1102 38-39 0.4076734251987659 1102 40-41 0.35862794814845245 1102 42-43 0.3370410758362965 1102 44-45 0.32015717442254754 1102 46-47 0.3458170437644412 1102 48-49 0.42110480511436776 1102 50-51 0.3400404648727573 1102 52-53 0.43122156499664754 1102 54-55 0.44427092140256264 1102 56-57 0.46901487797196495 1102 58-59 0.368858012060052 1102 60-61 0.32360183519020325 1102 62-63 0.36742579839501843 1102 64-65 0.3688799930646489 1102 66-67 0.35290737328277544 1102 68-69 0.2767111308135597 1102 70-71 0.5346037778700818 1102 72-73 0.42670899179140775 1102 74-75 0.45730715693712654 1102 76-77 0.5377766876529613 1102 78-79 0.5434786929748583 1102 80-81 0.6148090071918197 1102 82-83 0.6050754247494368 1102 84-85 0.6748617176302787 1102 86-87 0.6901345196183222 1102 88-89 0.6649924746871534 1102 90-91 0.5256782646709723 1102 92-93 0.5135707374924081 1102 94-95 0.5923075403039597 1102 96-97 0.645989490394463 1102 98-99 0.640133655068329 1102 100-101 0.5161561136826762 1102 102-103 0.4808377371260093 1102 104-105 0.446443676823872 1102 106-107 0.5833103172306942 1102 108-109 0.4810361489032644 1102 110-111 0.36354055397985263 1102 112-113 0.46091173085737225 1102 114-115 0.30760420400513766 1102 116-117 0.4722863744901069 1102 118-119 0.499261701820366 1102 120-121 0.5196281516506573 1102 122-123 0.5389365971926559 1102 124-125 0.4504445158469714 1103 1 -0.021584350598068625 1103 2 0.0177496000641284 1103 3 0.058357094566581225 1103 4 0.12650265413977024 1103 5 -0.015911147607674536 1103 6 0.10121054832213616 1103 7 0.1790431603207594 1103 8 0.1705037396021325 1103 9 0.2305626679197914 1103 10-11 0.18419084751030113 1103 12-13 0.05724714749931792 1103 14-15 0.08365981982942827 1103 16-17 0.21146663228380902 1103 18-19 0.2300748256762759 1103 20-21 0.08342221197544575 1103 22-23 0.10684776553346609 1103 24-25 0.19379681144499017 1103 26-27 0.10969569466774232 1103 28-29 0.1941375871146036 1103 30-31 0.09087423902964531 1103 32-33 0.2335172417135425 1103 34-35 0.2615076138502417 1103 36-37 0.19966226523609976 1103 38-39 0.2596538174687808 1103 40-41 0.2101876801722895 1103 42-43 0.21046446546924358 1103 44-45 0.27021834038426107 1103 46-47 0.28319810193558226 1103 48-49 0.2020484343332356 1103 50-51 0.2356044900546621 1103 52-53 0.2874587619798987 1103 54-55 0.239955258877103 1103 56-57 0.21163601254285425 1103 58-59 0.18191636784975884 1103 60-61 0.18609007805369515 1103 62-63 0.3525956493462701 1103 64-65 0.3451894351272138 1103 66-67 0.33940873797617144 1103 68-69 0.25759907592576425 1103 70-71 0.274718839122599 1103 72-73 0.1474355972536472 1103 74-75 0.08931715122445638 1103 76-77 0.21402669486592885 1103 78-79 0.15983972217872378 1103 80-81 0.1727320188652861 1103 82-83 0.20221476196430643 1103 84-85 0.22711995339621183 1103 86-87 0.2751500130718334 1103 88-89 0.21982112343873084 1103 90-91 0.1617771457955044 1103 92-93 0.22690664825934803 1103 94-95 0.20551304062421494 1103 96-97 0.21986556584786854 1103 98-99 0.3026591168424879 1103 100-101 0.18074736925830237 1103 102-103 0.29839842703189845 1103 104-105 0.3285745604412007 1103 106-107 0.21739562643617205 1103 108-109 0.22503524872496072 1103 110-111 0.3029669211256518 1103 112-113 0.09897802091098029 1103 114-115 0.26606270636233376 1103 116-117 0.3209462051873402 1103 118-119 0.10258716821082459 1103 120-121 0.0421499203856186 1103 122-123 0.019327424606547794 1103 124-125 -0.2442519336420581 1104 1 0.06664870452690508 1104 2 0.13802607996527883 1104 3 0.13524134481068018 1104 4 0.11159083313458495 1104 5 0.16686512543002152 1104 6 0.118329464467962 1104 7 0.15639630105962254 1104 8 0.08349366121466772 1104 9 0.2503575697010234 1104 10-11 0.25836297996910673 1104 12-13 0.1954134546832762 1104 14-15 0.3377375122262336 1104 16-17 0.2998284943858778 1104 18-19 0.38721657954738475 1104 20-21 0.25434162349029066 1104 22-23 0.18962734838641637 1104 24-25 0.2689224465157878 1104 26-27 0.44131711799862927 1104 28-29 0.3228538201035249 1104 30-31 0.432756365475619 1104 32-33 0.4155005381792165 1104 34-35 0.3867405420989698 1104 36-37 0.45284354933409077 1104 38-39 0.5662842048826491 1104 40-41 0.5962972129918711 1104 42-43 0.5046910443890269 1104 44-45 0.5171186983959473 1104 46-47 0.4205570900147251 1104 48-49 0.4979859119230241 1104 50-51 0.5489344951848807 1104 52-53 0.641004677392047 1104 54-55 0.5559692483629206 1104 56-57 0.6105101237195427 1104 58-59 0.5900899566272741 1104 60-61 0.5569667377881302 1104 62-63 0.4599111685183743 1104 64-65 0.47618668246419205 1104 66-67 0.5160635076957725 1104 68-69 0.5829489509802386 1104 70-71 0.5822161223159092 1104 72-73 0.5362164268036764 1104 74-75 0.6134142612041025 1104 76-77 0.5217377369612635 1104 78-79 0.4823963889741023 1104 80-81 0.484602119649157 1104 82-83 0.508385636693319 1104 84-85 0.4954836358966688 1104 86-87 0.5829291821978764 1104 88-89 0.6507218344973751 1104 90-91 0.6186292908316062 1104 92-93 0.47234103393005356 1104 94-95 0.6383877806658091 1104 96-97 0.7028578043310887 1104 98-99 0.6345232599589004 1104 100-101 0.7426684963607251 1104 102-103 0.7888278203979233 1104 104-105 0.7519528888741682 1104 106-107 0.7208883089862752 1104 108-109 0.7182070998494687 1104 110-111 0.7811847511004437 1104 112-113 0.7821445763310813 1104 114-115 0.8646267981766087 1104 116-117 1.1322155745020446 1104 118-119 1.2194832234626745 1104 120-121 1.0711549347557323 1104 122-123 0.9693919660040642 1104 124-125 1.0358422857655931 1105 1 0.14467950436843324 1105 2 0.1370478456781612 1105 3 0.20141910202684699 1105 4 0.24514214032870285 1105 5 0.2652721716245878 1105 6 0.1463036419904853 1105 7 0.20003832338766614 1105 8 0.23161671311872567 1105 9 0.18802213399474965 1105 10-11 0.2034925518773747 1105 12-13 0.29163167569880244 1105 14-15 0.24978080168959593 1105 16-17 0.26277138035355563 1105 18-19 0.23350165176485405 1105 20-21 0.3256950916268053 1105 22-23 0.36382885945723586 1105 24-25 0.4189547168241461 1105 26-27 0.4377211723747507 1105 28-29 0.3617915947451422 1105 30-31 0.4485654415582587 1105 32-33 0.3371241890181125 1105 34-35 0.4148665951676591 1105 36-37 0.3350961258962428 1105 38-39 0.2975119979518297 1105 40-41 0.37105721068648023 1105 42-43 0.4771774262150572 1105 44-45 0.4439533679744727 1105 46-47 0.4379469698591052 1105 48-49 0.410333212095054 1105 50-51 0.4304448286931972 1105 52-53 0.2897705393000578 1105 54-55 0.29396342153427923 1105 56-57 0.2428339649610578 1105 58-59 0.23868940447528786 1105 60-61 0.32199060431626947 1105 62-63 0.3034523558684157 1105 64-65 0.31136043587749285 1105 66-67 0.28681866406527945 1105 68-69 0.4034419208299589 1105 70-71 0.29160447146247037 1105 72-73 0.32171311695523386 1105 74-75 0.42900022302285024 1105 76-77 0.38273163130469356 1105 78-79 0.40803318886028705 1105 80-81 0.4040055317345086 1105 82-83 0.45566872596135255 1105 84-85 0.43613612439696325 1105 86-87 0.4856252457394916 1105 88-89 0.39093494834068565 1105 90-91 0.46630279111900563 1105 92-93 0.5344887670382512 1105 94-95 0.39295404227909714 1105 96-97 0.42378220457845117 1105 98-99 0.3908865857270136 1105 100-101 0.5503151315515069 1105 102-103 0.4996741747876996 1105 104-105 0.5342591540909325 1105 106-107 0.5359768459392811 1105 108-109 0.6511121181809791 1105 110-111 0.6002991583003308 1105 112-113 0.5412660658641286 1105 114-115 0.6353276947356044 1105 116-117 0.6286108019233403 1105 118-119 0.6860724206199791 1105 120-121 0.5211073990078674 1105 122-123 0.6872991054008892 1105 124-125 0.7501459506653099 1106 1 0.01906926363552941 1106 2 0.02625105171305364 1106 3 0.057984876893684145 1106 4 0.12546788900912276 1106 5 0.11656112217569614 1106 6 0.19207632662929086 1106 7 0.17715973889591652 1106 8 0.10758406417593847 1106 9 0.21059691194570007 1106 10-11 0.22615466795249262 1106 12-13 0.1604668303698631 1106 14-15 0.11205258391786543 1106 16-17 0.12645583797129945 1106 18-19 0.18665563413306074 1106 20-21 0.12598158069427257 1106 22-23 0.2144856721812758 1106 24-25 0.04737754545824657 1106 26-27 0.07398140895345051 1106 28-29 0.14886475156036738 1106 30-31 0.049025806066048006 1106 32-33 0.19212663648760753 1106 34-35 0.2746803972939986 1106 36-37 0.18351918476264473 1106 38-39 0.1795935182057704 1106 40-41 0.17276863751614968 1106 42-43 0.32817458234558927 1106 44-45 0.18523732348558042 1106 46-47 0.13070796570391252 1106 48-49 0.16676219894277722 1106 50-51 0.155041696933246 1106 52-53 0.18765975952326386 1106 54-55 0.1404540305587787 1106 56-57 0.09630436659630703 1106 58-59 0.07191487897906512 1106 60-61 0.07050160191284505 1106 62-63 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-0.9519080001380829 2216 84-85 -1.0039231561698188 2216 86-87 -1.260954136190211 2216 88-89 -1.0956584356741033 2216 90-91 -1.1593792397921234 2216 92-93 -1.405378365349943 2216 94-95 -1.353674803538162 2216 96-97 -1.2597326231730719 2216 98-99 -1.6311628933801998 2216 100-101 -1.4389245704475933 2216 102-103 -1.485378218883966 2216 104-105 -1.5529513870440006 2216 106-107 -1.5357610106763246 2216 108-109 -1.3772877631056915 2216 110-111 -1.4431510620042118 2216 112-113 -1.2473043516871805 2216 114-115 -1.3130719582562023 2216 116-117 -1.2138010572339475 2216 118-119 -0.9874659215027073 2216 120-121 -1.1669638137705576 2216 122-123 -1.1659453451314192 2216 124-125 -1.7288680438099533 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 20.0 4 65.0 5 195.0 6 589.0 7 1393.0 8 1914.0 9 3043.0 10 6068.0 11 15604.0 12 21074.0 13 10220.0 14 11586.0 15 15515.0 16 7915.0 17 3782.0 18 3221.0 19 3208.0 20 3375.0 21 3752.0 22 3462.0 23 3721.0 24 4759.0 25 6013.0 26 7693.0 27 9765.0 28 12497.0 29 16056.0 30 20782.0 31 27548.0 32 36744.0 33 47907.0 34 75509.0 35 121065.0 36 227683.0 37 222956.0 38 13985.0 39 66.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 62.024693193114146 15.650996264331333 9.387686847948272 12.936623694606247 2 16.486274057355477 27.72703252011583 38.820276630615616 16.966416791913083 3 34.53356504253897 21.46088052931887 22.732980550230387 21.272573877911775 4 14.377342455976782 19.052662845167397 39.79993853147993 26.770056167375895 5 16.766483108832208 33.038452343659834 28.139374804214135 22.05568974329382 6 30.468262291164045 39.989162884321 12.099042172038182 17.443532652476776 7 35.53185812721412 33.3219332953561 14.459746362889991 16.686462214539787 8 40.27351850154055 24.025374012239933 13.899255941497202 21.801851544722314 9 42.389290007695024 11.325594355745054 18.183438285791397 28.101677350768522 10-11 33.895403278060314 25.440918788121024 19.52723493108986 21.136443002728797 12-13 32.114885599588774 25.84122494094815 22.775782198840698 19.26810726062238 14-15 30.540959271990825 23.122779439138736 25.853895465085497 20.482365823784942 16-17 27.416541935829457 27.881951919996457 25.616863952564856 19.084642191609227 18-19 26.04755876084334 22.90320852695371 28.594849174407187 22.45438353779576 20-21 29.551576759168363 22.03419599343605 26.72960380404159 21.684623443354 22-23 30.024403635513313 20.171937982419905 27.320997942842546 22.482660439224233 24-25 22.876219280417242 22.08801996792194 34.186722320827975 20.849038430832845 26-27 20.703172884666294 23.3305039263173 31.598999543655104 24.3673236453613 28-29 21.72505580696302 21.40489329461441 30.382680732665335 26.48737016575724 30-31 21.513210824130034 22.85556529594945 30.742039459926634 24.88918441999388 32-33 24.166037432642774 21.913104927391718 28.598506114300488 25.32235152566502 34-35 23.557079681114843 18.749491376317657 31.31803324621919 26.375395696348313 36-37 23.193394433416394 22.064224105517653 30.714638163824727 24.027743297241226 38-39 22.25402443659948 22.541789551865193 29.10367888153266 26.10050713000267 40-41 22.37480646546973 22.963985837856434 28.673344616623936 25.987863080049898 42-43 22.262059403125605 20.500805041838483 26.244209415950642 30.99292613908527 44-45 22.553172421110535 21.684983986723765 26.407690081039846 29.35415351112585 46-47 24.23042017724312 19.944332103708756 26.354175143728035 29.471072575320083 48-49 20.11383899369255 19.66042995311906 28.62091130942141 31.604819743766978 50-51 23.08492238531373 22.032753819957 27.65171922530561 27.230604569423654 52-53 24.370826066667558 20.52403433609029 27.369413766782007 27.735725830460144 54-55 22.261029279211996 20.978988562534187 26.542636313722177 30.21734584453164 56-57 22.41503280429603 22.13288186435946 25.100926390435507 30.351158940909002 58-59 22.475037522263552 20.621020502556252 28.46196318955207 28.441978785628123 60-61 22.922729375116532 23.152395501654894 26.465789069767204 27.459086053461373 62-63 22.983918735584705 20.61118281918132 26.335787431870177 30.0691110133638 64-65 24.20415201744618 19.148509874252774 28.862011811400794 27.785326296900255 66-67 23.717675999245948 21.311770092824467 26.68319672173366 28.287357186195926 68-69 21.528147620877316 22.578616481776592 26.260639892372655 29.63259600497344 70-71 21.868655080519638 23.390920693850266 25.516735908162392 29.223688317467712 72-73 22.222193607669066 23.198287522006023 25.8624969997641 28.71702187056081 74-75 21.796494900371567 22.809261226032877 26.6248402020279 28.76940367156765 76-77 22.207050786139064 22.445627484530114 25.35629410861833 29.991027620712497 78-79 21.885652125094126 22.74075798577811 25.616760940173496 29.75682894895427 80-81 20.477575747586677 21.237498158653505 27.684168128584187 30.600757965175628 82-83 20.717646125652458 20.712701530867147 26.538052262306632 32.031600081173764 84-85 20.644146784416698 21.110535386301617 27.10729873696507 31.138019092316615 86-87 21.641306732786887 20.847390232571076 25.887219973690634 31.624083060951403 88-89 19.899727738249634 21.571876381009872 26.128938550018184 32.39945733072231 90-91 19.833799807778878 21.72670400522479 25.74717462763596 32.69232155936038 92-93 20.824727506471756 20.721045534567352 25.31184426174625 33.14238269721465 94-95 19.949328204689742 19.908638310102322 26.18090830145958 33.96112518374835 96-97 21.194748016238872 18.50148904411712 26.405423808429916 33.898339131214094 98-99 20.877109307478598 19.30699443836099 26.535476952522618 33.28041930163779 100-101 21.177905490251423 20.15035688642987 25.959534672425743 32.71220295089296 102-103 20.480666119327495 21.16667713959312 26.218301799523463 32.13435494155592 104-105 19.982807231881925 21.568425465899292 25.678671387381186 32.7700959148376 106-107 20.132844779898573 22.51155541500087 24.765518044165532 32.59008176093502 108-109 20.995213014173476 22.954766228829666 24.986531129829608 31.06348962716725 110-111 21.735070350643245 23.617543030117606 24.222903142897025 30.42448347634213 112-113 20.19870142044372 24.587922983498377 24.33033943752942 30.883036158528483 114-115 20.860715497957507 24.47101495183366 24.495635078220406 30.172634471988424 116-117 21.262779332198768 25.141134100058927 23.947675305035087 29.64841126270722 118-119 20.253591612120314 25.703179243747513 24.235578438583588 29.807650705548582 120-121 20.041967421166866 25.1520726693608 24.883415245690717 29.92254466378162 122-123 20.294014480787485 25.79742452750984 24.731788145986364 29.176772845716307 124-125 19.960710022344692 26.848924214033005 24.079562977568237 29.11080278605407 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 5.5 2 23.5 3 57.5 4 79.5 5 94.5 6 116.5 7 165.5 8 244.5 9 327.0 10 387.5 11 433.0 12 450.0 13 466.0 14 538.5 15 603.0 16 685.0 17 770.5 18 842.0 19 951.0 20 1086.5 21 1215.5 22 1302.5 23 1435.0 24 1599.0 25 1753.0 26 1971.5 27 2162.5 28 2328.0 29 2508.0 30 2758.0 31 3104.5 32 3495.5 33 3900.5 34 4404.5 35 4902.5 36 5309.0 37 5714.5 38 6036.0 39 6304.0 40 6485.0 41 6731.0 42 7071.0 43 7749.0 44 8251.0 45 10130.5 46 16806.5 47 27595.5 48 39866.0 49 57146.5 50 74218.0 51 81740.5 52 83335.5 53 80609.5 54 72372.0 55 60773.5 56 49744.0 57 41300.0 58 34273.0 59 27079.0 60 21691.5 61 17486.5 62 12544.0 63 8617.5 64 6497.0 65 6370.5 66 6327.5 67 5524.5 68 4889.5 69 3863.5 70 2746.5 71 2098.0 72 1739.0 73 1543.5 74 1355.0 75 1023.0 76 625.5 77 545.5 78 493.5 79 327.5 80 226.0 81 171.0 82 125.5 83 61.0 84 26.0 85 14.5 86 10.5 87 3.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20478863402478686 2 4.1204956544222706E-4 3 0.0 4 0.11897931202144306 5 0.03183082893041204 6 0.001957235435850578 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0012876548920069595 112-113 4.1204956544222706E-4 114-115 6.695805438436189E-4 116-117 0.0050476071766672815 118-119 0.0023692850012928053 120-121 4.1204956544222706E-4 122-123 0.002317778805612527 124-125 0.02889497577663617 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 970757.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.821027136038744 #Duplication Level Percentage of deduplicated Percentage of total 1 78.4394865748078 29.666619502827523 2 10.2682390641691 7.767106965705452 3 3.6719655588095987 4.166325271270125 4 1.9520499963199993 2.953141435268921 5 1.1973699196660974 2.2642880111784 6 0.7847318556341191 1.7807618883871235 7 0.554301354934753 1.4674972610571233 8 0.39827700889735623 1.2050596448933801 9 0.30732281293512714 1.0460938002788882 >10 2.0252900699220286 14.661170355713157 >50 0.2143752390505091 5.55869420654733 >100 0.15526512491492392 11.689661975390646 >500 0.016071302403312848 4.433576309484658 >1k 0.014981722579359433 10.69219745044418 >5k 2.7239495598835333E-4 0.6478059215531435 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 6288 0.6477419168751809 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 4784 0.49281128026890353 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 4474 0.46087743894713096 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 3833 0.39484649608501404 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 3766 0.3879446658638568 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 3708 0.3819699471649445 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3692 0.38032174890317555 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 3667 0.3777464391191616 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 3607 0.3715656956375282 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 3583 0.36909339824487486 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 3461 0.35652588649888695 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 3059 0.3151149051719431 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 2793 0.287713609070035 No Hit TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA 2169 0.22343387686104763 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2143 0.22075555468567312 No Hit ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC 2141 0.22054952990295204 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 1982 0.2041705596766235 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1819 0.18737953988485276 No Hit GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG 1775 0.18284699466498824 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 1667 0.17172165639804812 No Hit CGTCAACACCCGCCGCGGGCCTTCGCGATGCTTTGTTTTAATTAAACAGT 1628 0.16770417313498642 No Hit CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA 1624 0.16729212356954418 No Hit ACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGC 1609 0.16574693769913582 No Hit GTATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCC 1596 0.1644077766114486 No Hit CACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT 1589 0.1636866898719247 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 1589 0.1636866898719247 No Hit GGTATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGC 1525 0.15709389682484906 No Hit GTATCAACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC 1524 0.15699088443348852 No Hit ACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAA 1452 0.14957399225552842 No Hit CTAATAGGGAACGTGAGCTGGGATTAGACCGTCGTGAGACAGGTTAGTTT 1415 0.14576253377518783 No Hit CCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGT 1319 0.13587334420457436 No Hit GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1308 0.13474020789960825 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 1300 0.13391610876872378 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1289 0.13278297246375767 No Hit CCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTA 1273 0.13113477420198877 No Hit GTATCAACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA 1268 0.13061971224518598 No Hit GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG 1267 0.1305166998538254 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1255 0.12928055115749873 No Hit CTAATAGGGAACGTGAGCTGGGGTTAGACCGTCGTGAGACAGGTTAGTTT 1242 0.12794139006981148 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 1225 0.12619017941668204 No Hit GTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACA 1219 0.1255721050685187 No Hit TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCGTG 1181 0.12165763419681754 No Hit CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 1132 0.11661002702015025 No Hit GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1119 0.11527086593246302 No Hit GACCAGGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCG 1117 0.1150648411497419 No Hit AAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAG 1097 0.11300459332253077 No Hit GGTATCAACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCG 1096 0.11290158093117023 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 1094 0.1126955561484491 No Hit GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCCACTGACTCTGCGT 1091 0.11238651897436742 No Hit GGTATCAACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCT 1064 0.10960518440763241 No Hit ACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCA 1061 0.10929614723355072 No Hit GACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCC 1056 0.10878108527674794 No Hit ACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTAC 1017 0.10476360201368623 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCT 1014 0.10445456483960457 No Hit AAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACGTTCCCTATTAG 1005 0.10352745331735955 No Hit TTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA 1002 0.10321841614327788 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 999 0.1029093789691962 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 994 0.10239431701239343 No Hit GTCTCACGACGGTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACA 987 0.10167323027286952 No Hit TATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCG 983 0.10126118070742729 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.0301239136055676E-4 0.0 14-15 0.0 0.0 0.0 1.0301239136055676E-4 0.0 16-17 0.0 0.0 0.0 1.5451858704083513E-4 0.0 18-19 0.0 0.0 0.0 4.1204956544222706E-4 0.0 20-21 1.0301239136055676E-4 0.0 0.0 0.0015966920660886296 0.0 22-23 1.0301239136055676E-4 0.0 0.0 0.006747311634116468 0.0 24-25 1.0301239136055676E-4 0.0 0.0 0.014421734790477946 0.0 26-27 1.0301239136055676E-4 0.0 0.0 0.03023413686432341 0.0 28-29 1.0301239136055676E-4 0.0 0.0 0.0848307042854185 0.0 30-31 1.0301239136055676E-4 0.0 0.0 0.2477963074178193 0.0 32-33 2.0602478272111353E-4 0.0 0.0 0.4971378007060469 0.0 34-35 2.0602478272111353E-4 0.0 0.0 0.7910321532577154 0.0 36-37 2.0602478272111353E-4 0.0 0.0 1.2725120704769577 0.0 38-39 2.0602478272111353E-4 0.0 0.0 2.0745150434145723 0.0 40-41 2.0602478272111353E-4 0.0 0.0 3.0344360123079204 0.0 42-43 2.0602478272111353E-4 0.0 0.0 4.071461756134646 0.0 44-45 2.0602478272111353E-4 0.0 0.0 5.359116648141605 0.0 46-47 2.0602478272111353E-4 0.0 0.0 6.795521433273208 0.0 48-49 2.0602478272111353E-4 0.0 0.0 7.878748234625142 0.0 50-51 2.0602478272111353E-4 0.0 0.0 9.228416586231159 0.0 52-53 2.0602478272111353E-4 0.0 0.0 10.202656277523623 0.0 54-55 2.0602478272111353E-4 0.0 0.0 11.143365435428228 0.0 56-57 2.0602478272111353E-4 0.0 0.0 12.45770053679757 0.0 58-59 2.0602478272111353E-4 0.0 0.0 13.822563216129268 0.0 60-61 2.0602478272111353E-4 0.0 0.0 15.40849048732072 0.0 62-63 2.0602478272111353E-4 0.0 0.0 16.959805595015023 0.0 64-65 2.0602478272111353E-4 0.0 0.0 18.41191976982911 0.0 66-67 2.0602478272111353E-4 0.0 0.0 19.756643526649817 0.0 68-69 2.0602478272111353E-4 0.0 0.0 21.11851884663206 0.0 70-71 2.0602478272111353E-4 0.0 0.0 22.798856974505462 0.0 72-73 2.0602478272111353E-4 0.0 0.0 24.43726905909512 0.0 74-75 2.0602478272111353E-4 0.0 0.0 26.166898616234548 0.0 76-77 2.0602478272111353E-4 0.0 0.0 28.116820172298524 0.0 78-79 2.0602478272111353E-4 0.0 0.0 30.2205907348595 0.0 80-81 2.0602478272111353E-4 0.0 0.0 32.45163310694643 0.0 82-83 2.0602478272111353E-4 0.0 0.0 34.284172043055065 0.0 84-85 2.0602478272111353E-4 0.0 0.0 35.82904887628933 0.0 86-87 2.0602478272111353E-4 0.0 0.0 37.3290123068904 0.0 88-89 2.0602478272111353E-4 0.0 0.0 38.96464305691332 0.0 90-91 2.0602478272111353E-4 0.0 0.0 40.614644035531036 0.0 92-93 2.0602478272111353E-4 0.0 0.0 42.559569490614024 0.0 94-95 2.0602478272111353E-4 0.0 0.0 44.81059626662491 0.0 96-97 2.0602478272111353E-4 0.0 0.0 47.134967865284516 0.0 98-99 2.0602478272111353E-4 0.0 0.0 49.21973264163947 0.0 100-101 2.0602478272111353E-4 0.0 0.0 51.01281783185699 0.0 102-103 2.0602478272111353E-4 0.0 0.0 52.73065247018564 0.0 104-105 2.0602478272111353E-4 0.0 0.0 54.43190211350523 0.0 106-107 2.0602478272111353E-4 0.0 0.0 56.14293793400408 0.0 108-109 2.0602478272111353E-4 0.0 0.0 57.74776797901019 0.0 110-111 2.0602478272111353E-4 0.0 0.0 59.21909396481303 0.0 112-113 2.0602478272111353E-4 0.0 0.0 60.870331092127074 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATAGTC 240 0.0 64.45331 7 AGATAGT 245 0.0 63.13794 6 TAGGACG 40 1.760372E-4 59.57822 4 TCATAGG 145 0.0 57.523796 2 TAGATAG 270 0.0 57.294785 5 GGTATCA 11665 0.0 56.906204 1 TAGTCAA 285 0.0 56.36403 9 TATAAGG 75 2.2882887E-8 55.606335 2 GTACTAG 110 3.6379788E-12 54.268383 1 AGTATAA 55 1.3003993E-5 54.162018 2 CATAGGG 155 0.0 53.812584 3 TCGGGTA 45 3.1710978E-4 52.88749 5 CCTCTAG 305 0.0 50.887726 1 GTACAGG 365 0.0 50.70005 1 AGTAGAG 365 0.0 50.600677 2 GAGTAAG 1135 0.0 50.49111 1 GTCGAAG 60 2.1510521E-5 49.746017 1 GAGTAGA 700 0.0 49.46175 1 GTTGGAG 145 0.0 49.30611 3 GTCTTAG 85 6.042319E-8 49.160767 1 >>END_MODULE