##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935923_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1002562 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.07506069450069 31.0 31.0 34.0 28.0 34.0 2 30.951853351712913 31.0 31.0 34.0 28.0 34.0 3 31.226334131953934 33.0 31.0 34.0 28.0 34.0 4 34.403371562058005 37.0 35.0 37.0 32.0 37.0 5 34.608574831282255 37.0 35.0 37.0 32.0 37.0 6 34.7374795773229 37.0 35.0 37.0 32.0 37.0 7 34.72267550535528 37.0 35.0 37.0 32.0 37.0 8 34.72889856188445 37.0 35.0 37.0 32.0 37.0 9 36.32691245030232 39.0 38.0 39.0 32.0 39.0 10-11 36.008833867631125 39.0 37.0 39.0 31.0 39.0 12-13 35.802197769315015 39.0 37.0 39.0 30.5 39.0 14-15 37.27646968466788 40.0 38.0 41.0 31.0 41.0 16-17 37.43655504597222 40.0 38.0 41.0 31.5 41.0 18-19 37.4791125137398 40.0 38.0 41.0 31.0 41.0 20-21 37.615871636866345 40.0 38.0 41.0 32.0 41.0 22-23 37.54167772167706 40.0 38.0 41.0 31.0 41.0 24-25 37.47835894438449 40.0 38.0 41.0 31.0 41.0 26-27 37.43784424304931 40.0 37.5 41.0 31.0 41.0 28-29 37.32119060965805 40.0 37.0 41.0 31.0 41.0 30-31 37.2258862793523 40.0 37.0 41.0 30.0 41.0 32-33 37.138218883221185 40.0 37.0 41.0 30.0 41.0 34-35 36.87123639236277 40.0 36.0 41.0 30.0 41.0 36-37 36.86305884324361 40.0 36.0 41.0 30.0 41.0 38-39 36.99096365112581 40.0 36.0 41.0 30.0 41.0 40-41 36.94883059601301 40.0 36.0 41.0 30.0 41.0 42-43 36.7725138195942 40.0 35.0 41.0 30.0 41.0 44-45 36.54238141880502 40.0 35.0 41.0 30.0 41.0 46-47 36.345019061165296 39.5 35.0 41.0 30.0 41.0 48-49 36.15732194118668 39.0 35.0 41.0 30.0 41.0 50-51 35.889251737049676 39.0 35.0 41.0 30.0 41.0 52-53 35.606544034184424 38.5 35.0 41.0 29.5 41.0 54-55 35.30501305654912 38.0 35.0 40.0 29.0 41.0 56-57 34.9685745121 37.0 35.0 40.0 28.0 41.0 58-59 34.53924645059358 36.5 34.0 39.0 27.5 41.0 60-61 34.19146696164427 36.0 34.0 39.0 27.0 41.0 62-63 33.6994734490236 35.5 34.0 39.0 26.5 40.5 64-65 33.21525950514781 35.0 33.0 37.0 26.0 40.0 66-67 32.88365008847333 35.0 33.0 37.0 26.0 39.0 68-69 32.70941647499107 35.0 33.0 36.0 26.0 39.0 70-71 32.60567974848438 35.0 33.5 36.0 26.0 38.0 72-73 32.389720037264524 35.0 33.5 35.0 26.0 37.0 74-75 32.125494483134204 35.0 33.0 35.0 26.0 37.0 76-77 31.99644660380106 35.0 33.0 35.0 26.0 36.0 78-79 31.85398509019891 35.0 33.0 35.0 25.5 36.0 80-81 31.73577693948105 35.0 33.0 35.0 25.0 35.5 82-83 31.66661064353127 35.0 33.0 35.0 25.0 35.0 84-85 31.416514888854756 35.0 33.0 35.0 24.5 35.0 86-87 31.22460556055386 35.0 33.0 35.0 24.0 35.0 88-89 31.088682794680032 35.0 33.0 35.0 23.0 35.0 90-91 31.020806194529612 35.0 33.0 35.0 20.0 35.0 92-93 30.92857199853974 35.0 32.5 35.0 20.0 35.5 94-95 30.766448359303464 35.0 32.0 35.0 19.0 35.0 96-97 30.6529646046828 35.0 32.0 35.0 19.0 35.0 98-99 30.475678810886507 35.0 32.0 35.0 17.5 35.0 100-101 30.313047971098044 35.0 32.0 35.0 15.0 35.0 102-103 29.570656478103103 34.0 30.0 35.0 11.5 35.0 104-105 30.072090304639513 35.0 31.0 35.0 10.0 35.0 106-107 30.049655283164533 35.0 31.0 35.0 7.0 35.0 108-109 29.894339202962012 35.0 31.0 35.0 3.5 35.0 110-111 29.7706161813434 35.0 31.0 35.0 2.0 35.0 112-113 29.66234856298164 35.0 31.0 35.0 2.0 35.0 114-115 29.43911698229137 35.0 31.0 35.0 2.0 35.0 116-117 29.178200949168232 35.0 30.5 35.0 2.0 35.0 118-119 28.849474147234787 35.0 30.0 35.0 2.0 35.0 120-121 28.45146783939547 35.0 30.0 35.0 2.0 35.0 122-123 27.79533136105298 34.5 29.0 35.0 2.0 35.0 124-125 24.88206714397713 31.5 22.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2927389301925558 1101 2 0.3848580273380584 1101 3 0.3716397642646463 1101 4 0.35499279236372416 1101 5 0.33491799188688987 1101 6 0.3750449789919017 1101 7 0.2834774600018477 1101 8 0.36935402572574816 1101 9 0.4068776252919548 1101 10-11 0.5268546233040539 1101 12-13 0.3983615312948672 1101 14-15 0.5218265448224102 1101 16-17 0.46242569628134333 1101 18-19 0.5598537311382188 1101 20-21 0.44672948784169364 1101 22-23 0.3513187446509889 1101 24-25 0.4346936684690377 1101 26-27 0.42190426466257946 1101 28-29 0.4111587734151385 1101 30-31 0.33109033461209947 1101 32-33 0.29174888337264093 1101 34-35 0.09569338889734524 1101 36-37 0.11840345444418432 1101 38-39 0.12515772226198152 1101 40-41 0.07937995589300328 1101 42-43 -0.04162962462980602 1101 44-45 0.05017701714059797 1101 46-47 0.10954198312363417 1101 48-49 0.04038113402764765 1101 50-51 0.11189267536745007 1101 52-53 -0.018833164661700152 1101 54-55 -0.09950533491090141 1101 56-57 -0.03767881772592574 1101 58-59 -0.11650602873955052 1101 60-61 0.08105081826257532 1101 62-63 0.15358809704920873 1101 64-65 0.03962712540114666 1101 66-67 -0.012598386390983762 1101 68-69 -0.04015664673011088 1101 70-71 -0.07084841494400962 1101 72-73 0.08588149405627377 1101 74-75 0.10136898679712658 1101 76-77 0.19140919172454574 1101 78-79 0.29724776377553397 1101 80-81 0.252395304739359 1101 82-83 0.23513554428902594 1101 84-85 0.2715816362212138 1101 86-87 5.20886733820447E-4 1101 88-89 0.11086660537648996 1101 90-91 0.2643145821848769 1101 92-93 -0.11776814620119325 1101 94-95 -0.09021780614822461 1101 96-97 -0.10289679514380623 1101 98-99 -0.2253990670329422 1101 100-101 0.0852507460614973 1101 102-103 0.18152315018509668 1101 104-105 -0.025683913018163196 1101 106-107 0.16562316848497716 1101 108-109 0.1602501716630158 1101 110-111 0.07189165355745786 1101 112-113 0.07766227487879007 1101 114-115 -0.1519167044215024 1101 116-117 -0.17657880203703513 1101 118-119 -0.14867583523128403 1101 120-121 -0.3879449080873947 1101 122-123 -0.4174750229708657 1101 124-125 -0.13845647467909927 1102 1 0.2420928282259176 1102 2 0.23280915853476358 1102 3 0.23330069548070043 1102 4 0.3169149284390187 1102 5 0.3699913089121907 1102 6 0.3341505251035457 1102 7 0.24185052687858644 1102 8 0.23648505658661634 1102 9 0.29970347489876303 1102 10-11 0.4810843453765514 1102 12-13 0.3844209312147058 1102 14-15 0.4980057672887952 1102 16-17 0.4851159681278716 1102 18-19 0.5331831947699328 1102 20-21 0.5683859965416147 1102 22-23 0.5756619319030847 1102 24-25 0.5283363015245754 1102 26-27 0.43301998256133345 1102 28-29 0.34293455184707966 1102 30-31 0.2836008035370128 1102 32-33 0.37388053614984074 1102 34-35 0.4838811247072812 1102 36-37 0.3868239251657428 1102 38-39 0.247213639450365 1102 40-41 0.27346898667094877 1102 42-43 0.22306073013891137 1102 44-45 0.24351974832072187 1102 46-47 0.1740429989203065 1102 48-49 0.15988857629307773 1102 50-51 0.15362878935780344 1102 52-53 0.2549800116723162 1102 54-55 0.3149355623070562 1102 56-57 0.29761147899726126 1102 58-59 0.16034486336486964 1102 60-61 0.05575126189622637 1102 62-63 0.17106118189241926 1102 64-65 0.06482717515445557 1102 66-67 0.08732698364923408 1102 68-69 0.1354642330741882 1102 70-71 0.12030517074914826 1102 72-73 0.1589566630102084 1102 74-75 0.1176811611548203 1102 76-77 0.11909690680394647 1102 78-79 -0.004693997215106549 1102 80-81 0.05186183873054162 1102 82-83 0.253679707258744 1102 84-85 0.113274907086506 1102 86-87 0.2469158450654234 1102 88-89 0.005690879461930365 1102 90-91 0.16014221461850653 1102 92-93 0.18708003711210353 1102 94-95 0.22276642481768505 1102 96-97 0.1864173002443401 1102 98-99 0.18256975422830024 1102 100-101 0.26541990730367004 1102 102-103 0.3108154093998259 1102 104-105 0.04678315996717686 1102 106-107 -0.14035137440651724 1102 108-109 0.006363194590996102 1102 110-111 0.18919612997301272 1102 112-113 0.17851084516224702 1102 114-115 0.028628370692167948 1102 116-117 0.08928213747123337 1102 118-119 0.01482320425349215 1102 120-121 -0.21267921161020453 1102 122-123 -0.2688770987893747 1102 124-125 -0.4469919308201753 1103 1 0.3157512362385688 1103 2 0.3435471659148348 1103 3 0.4205964255808574 1103 4 0.3987889400362761 1103 5 0.5875637917798784 1103 6 0.5330171566270039 1103 7 0.6682527409007264 1103 8 0.5603385094068614 1103 9 0.50511465043909 1103 10-11 0.6852040080017545 1103 12-13 0.6128144472927204 1103 14-15 0.6726117240626479 1103 16-17 0.5954058995986173 1103 18-19 0.472210606472629 1103 20-21 0.46752938083259465 1103 22-23 0.5069982524091472 1103 24-25 0.5268392008392908 1103 26-27 0.49820843801731485 1103 28-29 0.4503963336077561 1103 30-31 0.5147460328464533 1103 32-33 0.544382644742349 1103 34-35 0.5957696328780813 1103 36-37 0.6006760601156671 1103 38-39 0.6519461117750893 1103 40-41 0.6467689339561389 1103 42-43 0.5968561966650014 1103 44-45 0.6109327688795005 1103 46-47 0.6212715176340637 1103 48-49 0.45165320465364545 1103 50-51 0.31630011249961143 1103 52-53 0.40808096053894616 1103 54-55 0.5124368801773755 1103 56-57 0.3307387852703201 1103 58-59 0.419070482763928 1103 60-61 0.5405773030137837 1103 62-63 0.2013181666051196 1103 64-65 0.19764478190194978 1103 66-67 0.37663114811944354 1103 68-69 0.5810151028685979 1103 70-71 0.4815927300533147 1103 72-73 0.3917241906854869 1103 74-75 0.3658045138169257 1103 76-77 0.4379661740680447 1103 78-79 0.35783631380229153 1103 80-81 0.4776815540705499 1103 82-83 0.43900258548752547 1103 84-85 0.4084435944876681 1103 86-87 0.43806502798582514 1103 88-89 0.4127111746648815 1103 90-91 0.3316366795631538 1103 92-93 0.3560534161316049 1103 94-95 0.47053527036327836 1103 96-97 0.31884745311729645 1103 98-99 0.34484571627363536 1103 100-101 0.2988542773616558 1103 102-103 0.2100022834114199 1103 104-105 0.07931742459553348 1103 106-107 -0.022366398128184528 1103 108-109 -0.03237823925191208 1103 110-111 -0.017158640717553908 1103 112-113 -0.004489945559946307 1103 114-115 0.005012134529806644 1103 116-117 0.14192842107692627 1103 118-119 0.10302694700520831 1103 120-121 0.03987745681362398 1103 122-123 0.1032486260392993 1103 124-125 -0.15387648538000676 1104 1 0.3079812013953642 1104 2 0.3227457721866145 1104 3 0.49411558934392374 1104 4 0.44699451494916786 1104 5 0.5304731994453817 1104 6 0.5067105364179412 1104 7 0.4346813123292961 1104 8 0.46120958954623603 1104 9 0.5761425250035543 1104 10-11 0.6173817083501874 1104 12-13 0.5088510326585833 1104 14-15 0.6734740934006282 1104 16-17 0.7590992793895595 1104 18-19 0.5823674008977093 1104 20-21 0.5712750254318948 1104 22-23 0.4850644544997351 1104 24-25 0.4329019186093177 1104 26-27 0.5465098317455386 1104 28-29 0.5956750792523522 1104 30-31 0.5762181582819892 1104 32-33 0.5415342126866065 1104 34-35 0.5444543018676313 1104 36-37 0.3797614259693276 1104 38-39 0.3940976797193443 1104 40-41 0.4340511639212963 1104 42-43 0.40281438481831344 1104 44-45 0.45149897097009273 1104 46-47 0.51685514132744 1104 48-49 0.5614005914132321 1104 50-51 0.6033645724299177 1104 52-53 0.5415043054866828 1104 54-55 0.5745971589230265 1104 56-57 0.5313746737720706 1104 58-59 0.5536436534956266 1104 60-61 0.6298978605050678 1104 62-63 0.45733733036819046 1104 64-65 0.4955367679646656 1104 66-67 0.46196215857240475 1104 68-69 0.4607712004295763 1104 70-71 0.4633436011334595 1104 72-73 0.3699123439955869 1104 74-75 0.4525989389040319 1104 76-77 0.5461717489809459 1104 78-79 0.48877708035281486 1104 80-81 0.4056258049416286 1104 82-83 0.5665896935014665 1104 84-85 0.6217362774144952 1104 86-87 0.4794935994143934 1104 88-89 0.4923540318077393 1104 90-91 0.37667152276872784 1104 92-93 0.3270725798946721 1104 94-95 0.3275300438824438 1104 96-97 0.29752341130544835 1104 98-99 0.3394014654025561 1104 100-101 0.31810514844179494 1104 102-103 0.3731294611117697 1104 104-105 0.2701101075223633 1104 106-107 0.23811269594846962 1104 108-109 0.18702071545192211 1104 110-111 0.26613404220927706 1104 112-113 0.1910762565306463 1104 114-115 0.24891457355419888 1104 116-117 0.07583086010131623 1104 118-119 0.05878478672646281 1104 120-121 0.21235132440944327 1104 122-123 0.15621900931456167 1104 124-125 0.27246497803463043 1105 1 0.038525961513293794 1105 2 0.041788924156595186 1105 3 0.1227942277786589 1105 4 0.01340432465165975 1105 5 0.03146489068097935 1105 6 0.21529737640722146 1105 7 0.16023076287874716 1105 8 0.2626736742420235 1105 9 0.13483992516436416 1105 10-11 0.23965455745230457 1105 12-13 0.20738862311689843 1105 14-15 0.1811831526340768 1105 16-17 0.24440315234587473 1105 18-19 0.22167489218691117 1105 20-21 0.2681475127007289 1105 22-23 0.31492407658497257 1105 24-25 0.32815788215796715 1105 26-27 0.38481558087445933 1105 28-29 0.2796957841572052 1105 30-31 0.27019932954974735 1105 32-33 0.3448359414456519 1105 34-35 0.40887402848247234 1105 36-37 0.3479837524233602 1105 38-39 0.25337468320365986 1105 40-41 0.22733212076932574 1105 42-43 0.18241938347819087 1105 44-45 0.1063585930553188 1105 46-47 0.15105173741428501 1105 48-49 0.13130979806023646 1105 50-51 0.17117648612597947 1105 52-53 0.24457821328620355 1105 54-55 0.3178764406169421 1105 56-57 0.4104503237318653 1105 58-59 0.3765430102364462 1105 60-61 0.317349281035753 1105 62-63 0.29109838638534313 1105 64-65 0.1509826939898602 1105 66-67 0.2305597195480189 1105 68-69 0.25492993803343467 1105 70-71 0.22554877400936135 1105 72-73 0.289608806070099 1105 74-75 0.22355176656417086 1105 76-77 0.2816749652768422 1105 78-79 0.18018521490119 1105 80-81 0.26797001560901634 1105 82-83 0.15915368438863098 1105 84-85 0.0684848032788743 1105 86-87 0.11074359941439482 1105 88-89 0.15087051532422535 1105 90-91 0.2023784378049136 1105 92-93 0.16358088865908016 1105 94-95 0.03438142420943535 1105 96-97 -0.01594650292665989 1105 98-99 0.08646659539451562 1105 100-101 0.19301636527374555 1105 102-103 0.27795558011471755 1105 104-105 0.2851965454746548 1105 106-107 0.1256143711025821 1105 108-109 0.0839267058030373 1105 110-111 0.1061517988428875 1105 112-113 0.08467214235214371 1105 114-115 0.19841872793639936 1105 116-117 0.21043116832967712 1105 118-119 0.06361760634586844 1105 120-121 -0.14453188384571902 1105 122-123 -0.20642170363102963 1105 124-125 0.02564274538922362 1106 1 0.16433908517124607 1106 2 0.17756339309536173 1106 3 0.23125034231996722 1106 4 -0.31051609232149247 1106 5 0.09879580182527548 1106 6 0.16384397011101726 1106 7 0.22809384975806068 1106 8 0.2668052340277427 1106 9 0.15698512276595267 1106 10-11 0.20512721866533212 1106 12-13 0.3502854701846445 1106 14-15 0.4608424301381788 1106 16-17 0.5006720060411993 1106 18-19 0.47689379009089805 1106 20-21 0.5931668772104572 1106 22-23 0.5131119392631973 1106 24-25 0.5675385150477297 1106 26-27 0.5587838750886931 1106 28-29 0.5355025830040603 1106 30-31 0.5067442459247928 1106 32-33 0.573206898581823 1106 34-35 0.48755244945299125 1106 36-37 0.5981263160801191 1106 38-39 0.6499565918291808 1106 40-41 0.6073262603349647 1106 42-43 0.49874840182292957 1106 44-45 0.4815845123355018 1106 46-47 0.6531335383044024 1106 48-49 0.6192956754350618 1106 50-51 0.41843596684134354 1106 52-53 0.5212759281811756 1106 54-55 0.5612014894693687 1106 56-57 0.49578442421556446 1106 58-59 0.6318085780020226 1106 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-1.4879205254393426 2216 78-79 -1.8101686123060858 2216 80-81 -1.6009883966683631 2216 82-83 -1.7834974145124711 2216 84-85 -1.6899061592069167 2216 86-87 -1.6090942495182823 2216 88-89 -1.659041698898335 2216 90-91 -1.6893403319310956 2216 92-93 -1.5324071750013957 2216 94-95 -1.4892717903920563 2216 96-97 -1.6211197061602078 2216 98-99 -1.5375229208363805 2216 100-101 -1.6062770855283262 2216 102-103 -1.5198458282470355 2216 104-105 -1.85540753435356 2216 106-107 -1.7322555606897616 2216 108-109 -2.040305168644359 2216 110-111 -2.3333162761855313 2216 112-113 -2.4743298470377795 2216 114-115 -2.4360798194931803 2216 116-117 -2.2935231388573882 2216 118-119 -2.424202117034202 2216 120-121 -2.2013376499187274 2216 122-123 -2.1996783033531457 2216 124-125 -1.9931704098463463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15897.0 3 3158.0 4 2180.0 5 1489.0 6 1884.0 7 2202.0 8 2868.0 9 3055.0 10 3921.0 11 4158.0 12 3667.0 13 3230.0 14 2990.0 15 3018.0 16 2950.0 17 2889.0 18 2765.0 19 2733.0 20 2780.0 21 3070.0 22 3934.0 23 5452.0 24 7878.0 25 11284.0 26 12641.0 27 12203.0 28 13910.0 29 17157.0 30 20398.0 31 26950.0 32 36572.0 33 50113.0 34 80444.0 35 109873.0 36 221266.0 37 277410.0 38 24122.0 39 51.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 75.40462999406617 8.326756826414181 5.4364569576779305 10.832156221841728 2 10.12368167978835 35.321005734169184 43.72054604294442 10.834766543098047 3 42.49585782643869 19.09499617453688 16.66415625844769 21.74498974057674 4 15.041294777574826 15.41588564553828 49.573285635762005 19.96953394112489 5 17.36763830162877 31.702071843732448 26.734075650075756 24.196214204563024 6 30.298874284084178 38.81844713843134 14.18196580361115 16.700712773873335 7 48.0930817108203 21.092602936482603 15.168967443345036 15.645347909352058 8 46.02577401851261 21.128909990424514 14.960800351101181 17.8845156399617 9 62.92648235221363 9.358323973978667 8.258940594197666 19.45625307961004 10-11 43.285751903622916 25.226419912184983 16.84454427756089 14.643283906631211 12-13 29.40217163626788 30.619103855921132 22.12167427051893 17.857050237292057 14-15 25.485954983332704 30.66997352782172 20.498283397934493 23.345788090911086 16-17 23.358405764431524 34.85495161396502 20.43549426369641 21.351148357907043 18-19 23.113782489262512 26.271442564150643 25.008129173058624 25.606645773528218 20-21 23.930041234357578 28.356101667527795 21.5120361633495 26.20182093476513 22-23 26.03928734582001 23.60312878405525 21.829871868273486 28.527712001851256 24-25 22.794251128608504 22.38689477558495 33.48431318960822 21.33454090619832 26-27 18.35414692534038 22.053513540471798 32.09635429654381 27.495985237644007 28-29 22.199085065378124 23.487993727530636 22.98833089270965 31.324590314381588 30-31 22.47172743431329 28.023154677715695 20.73796932259551 28.767148565375507 32-33 30.71670380485197 24.59453879161588 18.191493393924766 26.497264009607385 34-35 28.770689493517608 18.3860948250582 25.286515946145975 27.55669973527822 36-37 25.18946459171602 25.389053245584815 28.389615804309358 21.031866358389806 38-39 22.54598718084268 30.057093725874314 24.02459897741985 23.37232011586316 40-41 20.6995677075333 29.480870011031733 25.276292139538505 24.54327014189646 42-43 21.760349983342675 22.428837318789263 22.394575098597393 33.41623759927067 44-45 23.960213931906456 23.24624312511346 21.548143655953446 31.245399287026636 46-47 25.9414380357524 20.980946814261863 23.35282007496793 29.724795075017806 48-49 18.074343531871346 21.914654654774466 26.392981182211173 33.61802063114301 50-51 23.75439124961848 23.927497750762548 24.90040516197502 27.417705837643958 52-53 25.89650315890688 20.594337307817373 28.110730308948472 25.398429224327273 54-55 21.51941725299782 22.925863936594446 28.37814519201805 27.17657361838969 56-57 20.4081154285606 25.54491778293458 26.047381726471098 27.999585062033727 58-59 22.33243430331491 22.83295197703484 31.63659703838765 23.198016681262608 60-61 25.335390728952422 28.871231903862306 27.286791240840962 18.506586126344306 62-63 27.50079296841492 25.188467147168954 25.176597557058816 22.134142327357313 64-65 31.981951878325297 18.76240596403941 27.92616169696631 21.32948046066898 66-67 31.636812305280028 23.336373878310706 23.51800862091752 21.50880519549175 68-69 23.82088834624117 28.72960582229085 24.53660478088603 22.91290105058195 70-71 24.84999279207198 28.831911617334345 21.592857317443634 24.725238273150044 72-73 22.784909239891256 28.912070643118298 22.50761924093359 25.795400876056856 74-75 20.924865582012426 29.07673047572496 25.047582471295897 24.950821470966712 76-77 22.654760450148746 28.66638571852487 22.280417831396203 26.398435999930182 78-79 23.034565788108747 30.123973619323486 19.67164795442032 27.16981263814745 80-81 21.688995461094635 22.7415068208262 25.461608321542617 30.107889396536546 82-83 24.83797752058103 18.50350327635761 28.327389392072206 28.331129810989154 84-85 26.378649145315347 20.052915225897333 31.633807481957543 21.934628146829777 86-87 28.494445076176966 22.652448695006854 24.175389562847744 24.677716665968436 88-89 23.142475475598307 25.379775312285197 26.577637576581083 24.900111635535417 90-91 19.73994599829836 27.800253550656766 26.06599498683871 26.393805464206167 92-93 23.57266023919223 23.10224652352243 26.525178431498713 26.799914805786624 94-95 22.282199898245228 22.353478117736255 27.808481559441745 27.555840424576772 96-97 22.119511818014054 20.61381852278565 28.06762128489419 29.19904837430611 98-99 21.66235507284324 23.423001517661078 29.40192782357028 25.5127155859254 100-101 20.727661552395183 28.14726189312496 25.859305334176515 25.265771220303346 102-103 21.553430112052922 26.827468026915042 25.751325105080785 25.867776755951255 104-105 21.51109612720274 23.758200281879237 25.675048899962793 29.05565469095523 106-107 20.726628188062723 26.10936029528746 23.02541971784906 30.138591798800757 108-109 23.296115352466977 26.44135724274409 24.843201717200532 25.4193256875884 110-111 24.379439875040145 27.77903012482021 22.52005362261885 25.321476377520792 112-113 21.181786444566356 28.918457932272705 24.273203331504664 25.626552291656274 114-115 22.88946068248481 25.277244803857563 26.127969268781946 25.705325244875677 116-117 26.996584749870834 24.883498476902176 23.340152529220138 24.779764244006856 118-119 24.41965798613797 25.993422848737996 24.104525696956934 25.482393468167096 120-121 21.912702691514898 25.574752914972425 25.88506487774297 26.627479515769704 122-123 22.720009097220558 26.786526537549975 27.515301684581807 22.978162680647657 124-125 23.374649532243033 28.87614339480768 22.424536942601282 25.32467013034801 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7.0 1 11.5 2 17.5 3 20.5 4 22.5 5 26.0 6 31.5 7 34.5 8 45.0 9 50.0 10 49.5 11 65.5 12 93.5 13 120.0 14 154.0 15 183.5 16 214.5 17 272.5 18 356.0 19 396.5 20 461.5 21 580.5 22 686.0 23 797.0 24 945.0 25 1147.0 26 1378.0 27 1583.5 28 1794.5 29 2060.0 30 2441.0 31 2732.5 32 3082.0 33 3563.5 34 4080.0 35 4465.0 36 4778.5 37 5358.0 38 5826.5 39 5974.0 40 6069.0 41 6542.5 42 7085.0 43 7894.0 44 9156.5 45 12973.0 46 28464.5 47 58741.0 48 86827.0 49 109414.0 50 113914.0 51 93570.5 52 70450.5 53 52588.0 54 42253.5 55 37907.5 56 34489.0 57 29894.5 58 25295.0 59 20250.5 60 16841.5 61 14475.0 62 11041.0 63 7889.0 64 5852.5 65 5123.0 66 4662.5 67 4107.5 68 3900.5 69 3944.5 70 3621.0 71 2777.5 72 1951.0 73 1385.0 74 1025.5 75 827.5 76 648.5 77 550.0 78 467.5 79 330.0 80 367.0 81 412.5 82 338.0 83 179.5 84 54.0 85 33.0 86 23.0 87 13.0 88 9.5 89 6.0 90 3.5 91 3.5 92 3.5 93 1.5 94 1.0 95 2.5 96 2.0 97 0.5 98 1.0 99 1.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15201054897352984 2 0.014961668206056085 3 0.006882367374785799 4 0.013465501385450475 5 0.002493611367676014 6 0.0 7 1.9948890941408115E-4 8 1.9948890941408115E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0011969334564844868 28-29 0.008578023104805488 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.493611367676014E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.001994889094140811 66-67 4.987222735352029E-5 68-69 0.00698211182949284 70-71 0.019300551985812348 72-73 1.4961668206056085E-4 74-75 0.009176489833047731 76-77 4.4885004618168254E-4 78-79 9.974445470704056E-4 80-81 7.480834103028042E-4 82-83 4.987222735352029E-5 84-85 0.020347868760236275 86-87 0.002792844731797136 88-89 0.019250679758458827 90-91 9.974445470704057E-5 92-93 0.01461256261458144 94-95 0.015161157115470164 96-97 0.006682878465371718 98-99 0.003241694777978818 100-101 7.979556376563246E-4 102-103 0.0 104-105 4.987222735352028E-4 106-107 0.0013465501385450476 108-109 0.0 110-111 0.0 112-113 0.0011969334564844868 114-115 0.006333772873897076 116-117 0.0 118-119 0.07585565780470435 120-121 0.0022442502309084128 122-123 0.005785178373008353 124-125 0.2463688031263902 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1002562.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.45360089566119 #Duplication Level Percentage of deduplicated Percentage of total 1 81.03052033001738 29.538542484782138 2 9.330957687763044 6.802940150480312 3 2.98070035619382 3.2597178352273404 4 1.4837385422951783 2.1635045061735414 5 0.901269492933456 1.6427259197415573 6 0.631067330726747 1.3802805967561838 7 0.4631638527164533 1.1818793165355672 8 0.339932400843801 0.9913408057491073 9 0.27900355670346194 0.9153615874084179 >10 2.107005615319158 15.476190222163163 >50 0.2563049025258859 6.500036388152094 >100 0.17004827579949183 12.440766724836926 >500 0.01423914708783701 3.575381586840434 >1k 0.010405530564188586 7.807084965335228 >5k 5.476595033783466E-4 1.5585022255664667 >10k+ 0.0010953190067566933 4.765744684251573 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 13624 1.3589184509287207 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 11882 1.185163610829056 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 11401 1.1371865281149693 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 10836 1.0808309112054917 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 8072 0.8051372383952313 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 7541 0.7521729329457928 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 4955 0.49423377307338595 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 4491 0.44795234608931916 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 3967 0.3956862518228299 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3791 0.3781312277943908 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3674 0.36646112659366703 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 3531 0.3521976695705602 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 3340 0.3331464787215155 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 2808 0.2800824288173699 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 2556 0.2549468262311957 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2379 0.2372920577480495 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 2304 0.22981122364502146 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2294 0.22881377909795106 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 2181 0.21754265571605544 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 2123 0.21175747734304712 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2027 0.20218200969117123 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1807 0.18023822965562228 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1702 0.16976506191138302 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1691 0.16866787290960558 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1652 0.164777839176031 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1638 0.16338141681013243 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1571 0.15669853834476072 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1540 0.15360646024884245 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1525 0.15211029342823687 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1524 0.15201054897352984 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1433 0.14293380359518912 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1378 0.13744785858630187 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1367 0.13635066958452444 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1323 0.13196191357741466 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1294 0.12906932439091048 No Hit GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1292 0.12886983548149641 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA 1254 0.12507954620262887 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1243 0.12398235720085142 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 1191 0.11879564555608531 No Hit CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 1129 0.1126114893642488 No Hit ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA 1088 0.10852196672126013 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 1080 0.1077240110836038 No Hit GAGTAAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 1039 0.10363448844061514 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCACGT 1029 0.10263704389354475 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 9.974445470704056E-5 0.0 0.0 0.0 0.0 3 9.974445470704056E-5 0.0 0.0 0.0 0.0 4 9.974445470704056E-5 0.0 0.0 0.0 0.0 5 9.974445470704056E-5 0.0 0.0 0.0 0.0 6 9.974445470704056E-5 0.0 0.0 0.0 0.0 7 9.974445470704056E-5 0.0 0.0 0.0 0.0 8 9.974445470704056E-5 0.0 0.0 0.0 0.0 9 9.974445470704056E-5 0.0 0.0 0.0 0.0 10-11 9.974445470704056E-5 0.0 0.0 0.0 0.0 12-13 9.974445470704056E-5 0.0 0.0 0.0 0.0 14-15 9.974445470704056E-5 0.0 0.0 0.0 0.0 16-17 9.974445470704056E-5 0.0 0.0 0.0 0.0 18-19 9.974445470704056E-5 0.0 0.0 4.987222735352028E-5 0.0 20-21 9.974445470704056E-5 0.0 0.0 2.992333641211217E-4 0.0 22-23 9.974445470704056E-5 0.0 0.0 0.0014462945932520882 0.0 24-25 9.974445470704056E-5 0.0 0.0 0.0028925891865041763 0.0 26-27 9.974445470704056E-5 0.0 0.0 0.00528645609947315 0.0 28-29 9.974445470704056E-5 0.0 0.0 0.011570356746016704 0.0 30-31 9.974445470704056E-5 0.0 0.0 0.03725455383307965 0.0 32-33 9.974445470704056E-5 0.0 0.0 0.09191451501253788 0.0 34-35 9.974445470704056E-5 0.0 0.0 0.1532074824300143 0.0 36-37 9.974445470704056E-5 0.0 0.0 0.2329033017409397 0.0 38-39 9.974445470704056E-5 0.0 0.0 0.3626708373147995 0.0 40-41 9.974445470704056E-5 0.0 0.0 0.5791163040290774 0.0 42-43 9.974445470704056E-5 0.0 0.0 1.0917529289959125 0.0 44-45 9.974445470704056E-5 0.0 0.0 1.8307097216930224 0.0 46-47 9.974445470704056E-5 0.0 0.0 2.570264981118375 0.0 48-49 9.974445470704056E-5 0.0 0.0 2.831046857949932 0.0 50-51 9.974445470704056E-5 0.0 0.0 3.171125576273587 0.0 52-53 9.974445470704056E-5 0.0 0.0 3.4308601363307205 0.0 54-55 9.974445470704056E-5 0.0 0.0 3.6935870300290654 0.0 56-57 9.974445470704056E-5 0.0 0.0 4.0979510494114075 0.0 58-59 9.974445470704056E-5 0.0 0.0 4.565652797532721 0.0 60-61 9.974445470704056E-5 0.0 0.0 5.258378035473118 0.0 62-63 9.974445470704056E-5 0.0 0.0 6.16241190071038 0.0 64-65 9.974445470704056E-5 0.0 0.0 7.030487890025754 0.0 66-67 9.974445470704056E-5 0.0 0.0 7.688701546637515 0.0 68-69 9.974445470704056E-5 0.0 0.0 8.202535105060836 0.0 70-71 9.974445470704056E-5 0.0 0.0 8.836660475860844 0.0 72-73 9.974445470704056E-5 0.0 0.0 9.483603009090709 0.0 74-75 9.974445470704056E-5 0.0 0.0 10.253181349382881 0.0 76-77 9.974445470704056E-5 0.0 0.0 11.23496601706428 0.0 78-79 9.974445470704056E-5 0.0 0.0 12.430702540092284 0.0 80-81 9.974445470704056E-5 0.0 0.0 14.610168747668475 0.0 82-83 9.974445470704056E-5 0.0 0.0 16.079404565503182 0.0 84-85 9.974445470704056E-5 0.0 0.0 16.813224518782878 0.0 86-87 9.974445470704056E-5 0.0 0.0 17.45827190737331 0.0 88-89 9.974445470704056E-5 0.0 0.0 18.16790383038655 0.0 90-91 9.974445470704056E-5 0.0 0.0 19.009348050295145 0.0 92-93 9.974445470704056E-5 0.0 0.0 20.193015494303594 0.0 94-95 9.974445470704056E-5 0.0 0.0 21.961983398532958 0.0 96-97 9.974445470704056E-5 0.0 0.0 24.128383082542527 0.0 98-99 9.974445470704056E-5 9.974445470704056E-5 0.0 26.17982728250223 0.0 100-101 9.974445470704056E-5 9.974445470704056E-5 0.0 27.73444435356616 0.0 102-103 9.974445470704056E-5 9.974445470704056E-5 0.0 29.032169581532116 0.0 104-105 9.974445470704056E-5 9.974445470704056E-5 0.0 30.556863316183936 0.0 106-107 9.974445470704056E-5 9.974445470704056E-5 0.0 32.000315192476876 0.0 108-109 9.974445470704056E-5 9.974445470704056E-5 0.0 33.097853299845795 0.0 110-111 9.974445470704056E-5 9.974445470704056E-5 0.0 34.20062799108683 0.0 112-113 9.974445470704056E-5 9.974445470704056E-5 0.0 37.378137212461674 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACGT 85 0.0 104.99151 2 GAGTACG 405 0.0 98.55195 1 AGTAGTA 1920 0.0 97.91916 2 GAGTAGT 2395 0.0 97.50481 1 GAGTTAG 1155 0.0 94.9033 1 GAGTCGG 730 0.0 90.58276 1 GAGTAAG 1020 0.0 90.526764 1 GAGTCGA 320 0.0 89.35867 1 AGTCCGT 270 0.0 88.141014 2 AGTCGGT 320 0.0 87.38356 2 GAGTCGT 815 0.0 86.25214 1 GAGTCAG 5710 0.0 85.341965 1 AGTCGTA 495 0.0 85.33653 2 GAGTCCG 700 0.0 85.1035 1 AGTTAGG 280 0.0 84.993126 2 AGTAAGG 460 0.0 84.06928 2 GAGTAAA 390 0.0 84.01243 1 AGTCGAG 115 0.0 82.77591 2 AGTTAGT 900 0.0 81.31008 2 GAGTGCG 235 0.0 81.11993 1 >>END_MODULE