FastQCFastQC Report
Thu 26 May 2016
SRR935922_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935922_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445489
Sequences flagged as poor quality0
Sequence length125
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT11940.2680200857933641No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA11920.26757114092603856No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT10610.23816525211621387No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA7910.17755769502726218No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC7720.17329271878766928No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC6730.15106994785505365No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA4950.1111138546630781No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGTG507.4553154E-659.485479
TATAAGG1006.91216E-1153.542932
GGTACCG350.007800666650.987548
GTATTCG505.285469E-447.6793561
ATAAGGG1152.746674E-1046.5590673
GTATAAG1301.8189894E-1145.845531
GTACAAG1501.8189894E-1243.7060741
GTATAGG2050.043.609161
TATTCGG558.528324E-443.267012
TACTAGG1109.618816E-943.267012
ACTAGGG1309.1495167E-1041.186873
GTACTAG1351.3005774E-939.732791
GTCTGTA600.001308499439.6614272
TCTGTAC600.001308499439.6614273
AGTTAGA801.1972223E-437.182592
ACAACAC4550.036.6064385
GTATTGG1652.3101165E-1036.1207241
GGGATTG1001.0625066E-535.691287
GTCTAGG1209.1514084E-734.7661931
TGTAAGG1209.281048E-734.7037472