##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935922_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 445489 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.147866726226685 31.0 31.0 34.0 16.0 34.0 2 29.048521961260548 31.0 31.0 34.0 15.0 34.0 3 28.99408739609732 31.0 30.0 34.0 15.0 34.0 4 32.17943652929702 37.0 35.0 37.0 16.0 37.0 5 32.30535209623582 37.0 35.0 37.0 16.0 37.0 6 32.481569690834114 37.0 35.0 37.0 17.0 37.0 7 32.4717018826503 37.0 35.0 37.0 16.0 37.0 8 32.309637274994444 37.0 35.0 37.0 12.0 37.0 9 33.568599898089516 39.0 35.0 39.0 8.0 39.0 10-11 33.53782472743435 39.0 35.0 39.0 10.5 39.0 12-13 33.497618347478834 39.0 35.0 39.0 10.0 39.0 14-15 34.51216528354235 40.0 35.5 41.0 10.0 41.0 16-17 34.55543122276869 40.0 35.5 41.0 14.0 41.0 18-19 34.7020184561235 40.0 35.0 41.0 16.5 41.0 20-21 34.83691965458182 40.0 35.0 41.0 17.5 41.0 22-23 34.613025237435714 40.0 35.0 41.0 15.5 41.0 24-25 34.34618026483258 40.0 34.0 41.0 13.5 41.0 26-27 34.1800571955761 40.0 34.0 41.0 8.5 41.0 28-29 34.0206862571242 40.0 34.0 41.0 2.0 41.0 30-31 33.843402418466 40.0 34.0 41.0 2.0 41.0 32-33 33.54468011555841 39.0 34.0 41.0 2.0 41.0 34-35 33.30143056281973 39.0 33.5 41.0 2.0 41.0 36-37 33.171881909542094 39.0 33.5 41.0 2.0 41.0 38-39 33.042207551701615 39.0 33.5 41.0 2.0 41.0 40-41 32.80870122494607 39.0 33.0 41.0 2.0 41.0 42-43 32.54762968333674 38.5 33.0 41.0 2.0 41.0 44-45 32.230178522926494 38.0 33.0 41.0 2.0 41.0 46-47 31.883074554029392 37.0 33.0 40.0 2.0 41.0 48-49 31.507519826527705 36.5 32.0 40.0 2.0 41.0 50-51 31.120505781287527 36.0 32.0 39.0 2.0 41.0 52-53 30.76122081577772 35.0 32.0 39.0 2.0 41.0 54-55 30.379178834943176 35.0 31.5 38.5 2.0 41.0 56-57 30.014374092289593 35.0 31.0 37.0 2.0 40.0 58-59 29.663725703664962 35.0 31.0 36.5 2.0 39.0 60-61 29.357992004291916 35.0 31.0 36.0 2.0 39.0 62-63 29.042970757976065 35.0 30.0 35.5 2.0 38.0 64-65 28.82162634767637 35.0 30.0 35.0 2.0 37.0 66-67 28.650312353391442 35.0 30.0 35.0 2.0 36.5 68-69 28.505761085009954 35.0 30.0 35.0 2.0 36.0 70-71 28.296076895276876 35.0 29.5 35.0 2.0 35.5 72-73 28.09949516149669 35.0 29.0 35.0 2.0 35.0 74-75 27.912473708666205 35.0 29.0 35.0 2.0 35.0 76-77 27.69705649297738 35.0 29.0 35.0 2.0 35.0 78-79 27.417846456365925 35.0 27.5 35.0 2.0 35.0 80-81 27.077946930227235 34.0 26.0 35.0 2.0 35.0 82-83 26.740620980540484 34.0 25.0 35.0 2.0 35.0 84-85 26.434801981642643 34.0 24.5 35.0 2.0 35.0 86-87 26.142915986702253 34.0 23.5 35.0 2.0 35.0 88-89 25.73719777592713 34.0 19.5 35.0 2.0 35.0 90-91 25.311273679035843 33.0 16.5 35.0 2.0 35.0 92-93 24.84664380040809 33.0 9.0 35.0 2.0 35.0 94-95 24.417911553371688 33.0 6.0 35.0 2.0 35.0 96-97 24.01411819371522 33.0 3.5 35.0 2.0 35.0 98-99 23.541022337251874 32.0 2.0 35.0 2.0 35.0 100-101 23.15824184211058 31.5 2.0 35.0 2.0 35.0 102-103 22.419076565302397 29.5 2.0 34.5 2.0 35.0 104-105 22.686220086242308 31.0 2.0 35.0 2.0 35.0 106-107 22.578909916967646 31.0 2.0 35.0 2.0 35.0 108-109 22.270100945253418 31.0 2.0 35.0 2.0 35.0 110-111 21.833004855338743 30.5 2.0 35.0 2.0 35.0 112-113 21.328891397991868 30.0 2.0 35.0 2.0 35.0 114-115 20.711876163047798 29.0 2.0 35.0 2.0 35.0 116-117 19.99043410723946 29.0 2.0 35.0 2.0 35.0 118-119 19.083642918231426 26.0 2.0 34.0 2.0 35.0 120-121 18.10597904774304 24.0 2.0 34.0 2.0 35.0 122-123 17.079049089876516 10.0 2.0 34.0 2.0 35.0 124-125 14.74753360913513 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.647776988372911 1101 2 0.8753674892213112 1101 3 0.6282361578125908 1101 4 0.474673103402381 1101 5 0.6246274106221961 1101 6 0.8086168218197898 1101 7 0.5020818251781805 1101 8 0.6913696619209162 1101 9 0.8834767033337556 1101 10-11 1.0004945463766362 1101 12-13 0.7125801403024283 1101 14-15 0.768584300746511 1101 16-17 1.1315884712327389 1101 18-19 1.018143053118088 1101 20-21 0.9413764589994926 1101 22-23 1.0819032042913648 1101 24-25 0.9662961381521811 1101 26-27 1.125720776900181 1101 28-29 0.9105924404482622 1101 30-31 0.7793889031903376 1101 32-33 0.7959871198719242 1101 34-35 0.3459976160690772 1101 36-37 0.5963064257028208 1101 38-39 0.5085760867627656 1101 40-41 0.5790776399183386 1101 42-43 0.7187066014275345 1101 44-45 0.5853786826251621 1101 46-47 0.5442846520204903 1101 48-49 0.47318328137724563 1101 50-51 0.7240323499685424 1101 52-53 0.7659280023261381 1101 54-55 0.6336452358236322 1101 56-57 0.47397761998457 1101 58-59 0.4145637643294293 1101 60-61 0.6469995494049847 1101 62-63 0.7482673858504825 1101 64-65 0.5391300552033371 1101 66-67 0.615175540235974 1101 68-69 0.6745384740299905 1101 70-71 0.564784035458576 1101 72-73 0.6654124242739421 1101 74-75 0.7107818090109426 1101 76-77 0.8532241994552976 1101 78-79 0.9174593615026971 1101 80-81 0.6902610766556627 1101 82-83 0.5547297839013119 1101 84-85 0.6446595024219128 1101 86-87 0.438421913110556 1101 88-89 0.49034888667988596 1101 90-91 0.22267310988937083 1101 92-93 0.05012122563463706 1101 94-95 0.30332094228093 1101 96-97 0.17744310981382228 1101 98-99 0.14493584791889447 1101 100-101 0.3335211524280446 1101 102-103 0.22207140065653874 1101 104-105 0.0352917168429947 1101 106-107 0.29251315123289245 1101 108-109 0.04660805788039113 1101 110-111 0.32292134579979503 1101 112-113 -0.07016253078655055 1101 114-115 -0.33629196939636685 1101 116-117 -0.27093897066411543 1101 118-119 -0.12017597538329028 1101 120-121 -0.028830761047228037 1101 122-123 0.3131677515098552 1101 124-125 0.7023090153320037 1102 1 0.3030321100426221 1102 2 0.10804879152005142 1102 3 0.2284994549175252 1102 4 0.17270498759974373 1102 5 0.10510254768096416 1102 6 -0.1412184672058885 1102 7 0.02286907149923678 1102 8 0.1850156655758184 1102 9 -0.011186420644321515 1102 10-11 0.04052412221582813 1102 12-13 0.19362635555682317 1102 14-15 0.34482925121299246 1102 16-17 0.5707205526023671 1102 18-19 0.46489752556205843 1102 20-21 0.15232973879173528 1102 22-23 0.4411594801401719 1102 24-25 0.6155579444424504 1102 26-27 0.4106918021959416 1102 28-29 0.22242818633650074 1102 30-31 0.41057458175146166 1102 32-33 0.4513083663853408 1102 34-35 0.5857632936683785 1102 36-37 0.5433073634733319 1102 38-39 0.4487511372580997 1102 40-41 0.46469969435712244 1102 42-43 0.2747864802387383 1102 44-45 0.313088598947175 1102 46-47 0.2161220810865636 1102 48-49 -0.004610793836754112 1102 50-51 0.0021113631454205972 1102 52-53 0.2249456276026578 1102 54-55 0.28412734604773604 1102 56-57 0.2596970582840825 1102 58-59 0.24147296890767578 1102 60-61 0.09159294172969012 1102 62-63 0.2144355112712759 1102 64-65 0.139438437428975 1102 66-67 0.1796442571735497 1102 68-69 0.2815675449158235 1102 70-71 0.3061637603792242 1102 72-73 0.12422866934463528 1102 74-75 0.2757524736257366 1102 76-77 0.06888436584944913 1102 78-79 0.1725209177208349 1102 80-81 0.4256865599681525 1102 82-83 0.4894555461404231 1102 84-85 0.5487754733750734 1102 86-87 0.3614676234116061 1102 88-89 0.2052005265760073 1102 90-91 0.4028603033105469 1102 92-93 0.31827550090634915 1102 94-95 0.7350981376257408 1102 96-97 0.5608770330609119 1102 98-99 0.19440624803431206 1102 100-101 0.29899196131522743 1102 102-103 0.12622945105569272 1102 104-105 0.27950278734410006 1102 106-107 0.0028442563593706893 1102 108-109 0.17069132709266555 1102 110-111 0.6069546005848281 1102 112-113 0.34009403360701995 1102 114-115 4.6683121919599557E-4 1102 116-117 0.20278542098732188 1102 118-119 0.0916910295219715 1102 120-121 -0.1243450921042637 1102 122-123 -0.5839480030347239 1102 124-125 -0.49685901390719955 1103 1 0.5314780559885683 1103 2 0.5002785212497827 1103 3 0.5884318873499552 1103 4 0.46083561823037655 1103 5 0.5510034485818593 1103 6 0.3785337850463648 1103 7 0.570616819246986 1103 8 0.5076508007109553 1103 9 0.6704577234998226 1103 10-11 0.5745781762698812 1103 12-13 0.5735587879892634 1103 14-15 0.6381513232850651 1103 16-17 0.6854615435933553 1103 18-19 0.43326464267918396 1103 20-21 0.2191878468998425 1103 22-23 0.41372704770773794 1103 24-25 0.5867291156136218 1103 26-27 0.48914900940314965 1103 28-29 0.5124844926427983 1103 30-31 0.8122862934631669 1103 32-33 0.7026597177366938 1103 34-35 0.8173736540287422 1103 36-37 0.8404582643742344 1103 38-39 0.7296970832040444 1103 40-41 0.9511861808436066 1103 42-43 0.782016209968468 1103 44-45 0.9205210313796073 1103 46-47 1.0802099189244068 1103 48-49 0.8880468638208967 1103 50-51 0.9331248766589404 1103 52-53 1.0197091411161736 1103 54-55 0.9989922109126006 1103 56-57 0.8738412024282276 1103 58-59 0.9180720680067722 1103 60-61 1.0003091579459102 1103 62-63 0.806485060820826 1103 64-65 0.7927154644560019 1103 66-67 0.9673582211375162 1103 68-69 1.0024121395104153 1103 70-71 1.0463439405594066 1103 72-73 1.073552993668958 1103 74-75 1.0995362574095182 1103 76-77 1.0647514829665639 1103 78-79 0.7706740708739872 1103 80-81 0.7851572806888747 1103 82-83 1.1149848254197003 1103 84-85 1.3175479958975949 1103 86-87 1.3015239297179129 1103 88-89 1.0325879139633969 1103 90-91 0.7500337267339745 1103 92-93 0.8583655909964385 1103 94-95 0.8278909304185333 1103 96-97 0.7330391952230713 1103 98-99 1.0219850768631389 1103 100-101 1.385478447801713 1103 102-103 1.499123595199837 1103 104-105 1.1702235080648187 1103 106-107 1.3613802923954061 1103 108-109 1.2310179036692404 1103 110-111 1.1278442402244657 1103 112-113 0.938900339913328 1103 114-115 0.6915816960840608 1103 116-117 0.6205556912575929 1103 118-119 0.5939995880805284 1103 120-121 0.3121864394272649 1103 122-123 0.35175019516347206 1103 124-125 0.15789323834505176 1104 1 -0.39913287648731455 1104 2 -0.3569562054383226 1104 3 -0.5587224534860553 1104 4 -0.6258578437203219 1104 5 -0.47523985259713086 1104 6 -0.560523020955781 1104 7 -0.6866447027251503 1104 8 -0.7226546655269885 1104 9 -0.8147244844315793 1104 10-11 -0.6692883832156582 1104 12-13 -0.5614412120107417 1104 14-15 -0.5195603594587723 1104 16-17 -0.7945652516906705 1104 18-19 -0.6161699768277842 1104 20-21 -0.4400243717496721 1104 22-23 -0.6723420841250629 1104 24-25 -0.7736942498740547 1104 26-27 -0.4990289112828137 1104 28-29 -0.4443697410120748 1104 30-31 -0.6220197086804546 1104 32-33 -0.6567454269578406 1104 34-35 -0.6758953706229178 1104 36-37 -0.6709779628497756 1104 38-39 -0.8689685116512607 1104 40-41 -0.6788379349120248 1104 42-43 -0.8120030826360392 1104 44-45 -1.2118744670126738 1104 46-47 -1.0249186984389418 1104 48-49 -0.9097880772294715 1104 50-51 -0.8575664416690394 1104 52-53 -0.8570995405558506 1104 54-55 -0.7374627730102326 1104 56-57 -0.764299204860091 1104 58-59 -0.7091331838688966 1104 60-61 -0.5565397166521642 1104 62-63 -0.6706344461459466 1104 64-65 -0.6630160467980168 1104 66-67 -0.6463502461722399 1104 68-69 -0.5397341627403875 1104 70-71 -0.6123966810911803 1104 72-73 -0.58581195809953 1104 74-75 -0.6253512023975532 1104 76-77 -0.5377056445789847 1104 78-79 -0.6364106022235454 1104 80-81 -0.806935430993871 1104 82-83 -0.6273999548589693 1104 84-85 -0.5338399998151608 1104 86-87 -0.4797676029723341 1104 88-89 -0.3522807891448565 1104 90-91 -0.39655255408060697 1104 92-93 -0.4973150092179246 1104 94-95 -0.6054825958408436 1104 96-97 -0.7566633771241911 1104 98-99 -0.7006060271025625 1104 100-101 -0.5392803946420166 1104 102-103 -0.4957370693232086 1104 104-105 -0.7217084319137435 1104 106-107 -0.36981477727233525 1104 108-109 -0.4294135004036441 1104 110-111 -0.6669574746737119 1104 112-113 -0.6927609676965325 1104 114-115 -1.1446160531120846 1104 116-117 -0.731727266941764 1104 118-119 -0.31012688566009317 1104 120-121 -0.15276800858988082 1104 122-123 0.3659249004153473 1104 124-125 0.567558297294676 1105 1 0.29951633350053086 1105 2 0.33568521981437627 1105 3 0.32111928766893527 1105 4 0.5512423167949692 1105 5 0.4509795251368871 1105 6 0.4508463847273845 1105 7 0.46459608559467114 1105 8 0.6422201787013861 1105 9 0.4498436884121091 1105 10-11 0.710905927466051 1105 12-13 0.6395157257883 1105 14-15 0.5843155976072367 1105 16-17 0.8637948769266899 1105 18-19 0.7054695868578094 1105 20-21 0.3418274003288744 1105 22-23 0.47513454372050035 1105 24-25 0.3303894026949621 1105 26-27 0.5562462980793796 1105 28-29 0.5345658323557245 1105 30-31 0.5161260063818247 1105 32-33 0.489860674674496 1105 34-35 0.5579159187815321 1105 36-37 0.7719096200361122 1105 38-39 0.9002792203651282 1105 40-41 0.8141247773348326 1105 42-43 0.8750305640354483 1105 44-45 0.5724747793859919 1105 46-47 0.8571397434858063 1105 48-49 0.7206305958783119 1105 50-51 0.7364183375839737 1105 52-53 0.6561724585005422 1105 54-55 0.7212609509444974 1105 56-57 0.7270070716467281 1105 58-59 0.7837538861885918 1105 60-61 0.8627334003701499 1105 62-63 0.7711620943136488 1105 64-65 0.6663159429249035 1105 66-67 0.7138335002443732 1105 68-69 0.9116984234019618 1105 70-71 0.8095098097779072 1105 72-73 0.7760051467790063 1105 74-75 0.6368887294988355 1105 76-77 0.7078814670176001 1105 78-79 0.5898287758181695 1105 80-81 0.3939650956809011 1105 82-83 0.425582911544101 1105 84-85 0.32144751742869815 1105 86-87 0.47623684837819624 1105 88-89 0.453532890039952 1105 90-91 0.5127370760162684 1105 92-93 0.6436127999278618 1105 94-95 0.5692585540230013 1105 96-97 0.6290399926712382 1105 98-99 0.5800034181709535 1105 100-101 0.7990350666214887 1105 102-103 0.7532783001440144 1105 104-105 0.7846134602179298 1105 106-107 0.5597136257287403 1105 108-109 0.7366518749611082 1105 110-111 0.480396074355248 1105 112-113 0.47245695873310467 1105 114-115 0.8135474058129297 1105 116-117 0.46478216653670046 1105 118-119 0.6072372276339593 1105 120-121 0.6355556579280623 1105 122-123 0.5722844854346043 1105 124-125 0.522139649828306 1106 1 -0.5004485495160189 1106 2 -0.36577652462177923 1106 3 -0.2926231585216037 1106 4 -1.2220848710662615 1106 5 -0.4557702517239477 1106 6 -0.44917263697198706 1106 7 -0.4201324162270197 1106 8 -0.3230066916437835 1106 9 -0.3978144477539942 1106 10-11 -0.5111175423845538 1106 12-13 -0.6050191936621161 1106 14-15 -0.5140046400176956 1106 16-17 -0.30210726374609465 1106 18-19 -0.24510691695384423 1106 20-21 -0.32929839163226404 1106 22-23 -0.07785552109960037 1106 24-25 -0.36502929417230945 1106 26-27 -0.3256216327986863 1106 28-29 -0.2517509813633154 1106 30-31 -0.1386082019496797 1106 32-33 -0.11744731590244584 1106 34-35 -0.05855906768379526 1106 36-37 -0.13960289770528078 1106 38-39 0.007846930298846644 1106 40-41 0.060704529467457746 1106 42-43 -0.04575137412939512 1106 44-45 0.07426720874964587 1106 46-47 0.051570775193514606 1106 48-49 -0.08663203526166541 1106 50-51 -0.1726396493349469 1106 52-53 -0.06266853472480705 1106 54-55 -0.012131642298410128 1106 56-57 -0.0025043633210088956 1106 58-59 -0.04742487388924843 1106 60-61 0.032388668649272034 1106 62-63 0.06979545837434387 1106 64-65 0.22084237577098875 1106 66-67 0.17848971960846427 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2216 90-91 -2.282671900971657 2216 92-93 -2.1886041059732584 2216 94-95 -2.095539805512203 2216 96-97 -2.1472530125691343 2216 98-99 -2.1108828885048254 2216 100-101 -2.389196876873612 2216 102-103 -2.338690257614015 2216 104-105 -2.7594626391213275 2216 106-107 -2.7004595344444198 2216 108-109 -3.091828416677078 2216 110-111 -3.1979133355331086 2216 112-113 -3.6263810453680563 2216 114-115 -3.766242979240616 2216 116-117 -3.550483269781367 2216 118-119 -3.149290455209517 2216 120-121 -2.611265941525115 2216 122-123 -2.1877519693386915 2216 124-125 -2.009758276806462 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32048.0 3 3244.0 4 1881.0 5 3057.0 6 7422.0 7 6433.0 8 4115.0 9 3834.0 10 2863.0 11 2025.0 12 1663.0 13 1492.0 14 1599.0 15 1635.0 16 1566.0 17 1646.0 18 1938.0 19 2330.0 20 3007.0 21 3937.0 22 4885.0 23 6076.0 24 6898.0 25 7383.0 26 7708.0 27 8442.0 28 9622.0 29 11638.0 30 15288.0 31 20815.0 32 26921.0 33 34067.0 34 44534.0 35 57203.0 36 74291.0 37 20403.0 38 1578.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.740061368311025 18.653718627417923 10.982904541929393 13.623315462341662 2 20.355677093155876 23.307828398487665 35.15549927706083 21.180995231295633 3 31.084506125307836 24.21208359243636 24.370351579424714 20.33305870283109 4 17.38306594645078 22.633816919685152 28.09268513903669 31.890431994827377 5 16.80142948858968 34.4460058275006 24.916381202943384 23.83618348096634 6 32.18328623153434 36.348596710580956 8.756445164751543 22.711671893133165 7 28.806452264600313 41.83780895963294 11.327378801177138 18.028359974589605 8 36.38324713572531 28.424334662213123 10.296349172143806 24.896069029917754 9 36.73446482404728 17.586966232611804 14.66702881552631 31.0115401278146 10-11 30.475612192444707 28.00596647728676 14.326616369876696 27.191804960391842 12-13 32.90653641279583 29.380523424820815 16.80366967534552 20.90927048703784 14-15 36.495962863280575 22.796522473057696 20.50959731890125 20.197917344760477 16-17 30.31298191425602 23.93123062522307 21.742512160794096 24.013275299726818 18-19 29.222943776389542 24.702293434854734 21.556985694371804 24.517777094383923 20-21 38.54764090695842 22.56441797665038 17.428713166879543 21.45922794951166 22-23 34.006788046393964 25.58357220941482 16.852716902100838 23.556922842090376 24-25 27.507749910772205 27.55960304294831 18.15353465517667 26.77911239110281 26-27 24.672155802875963 26.87525533911279 21.045666979433697 27.406921878577556 28-29 26.530955849835113 25.10691267619546 23.083821783022827 25.2783096909466 30-31 27.559827515381976 26.286507635429828 20.148645645571495 26.005019203616698 32-33 24.64718545239052 26.206034267961726 21.736451405085198 27.410328874562563 34-35 23.56870764485767 26.75531831313456 21.95284283113612 27.72313121087165 36-37 24.40284720834858 28.174545274967507 19.763226476972495 27.659381039711416 38-39 25.168634915789166 24.871882358486967 21.52825322286297 28.4312295028609 40-41 23.637283973341656 25.160890616827803 22.819755369941795 28.382070039888752 42-43 23.577462069770522 25.169869514174316 24.732597213399206 26.52007120265596 44-45 23.944474498809175 24.581639501760986 25.28502387264332 26.18886212678652 46-47 21.507040577881835 25.063806289268648 24.709701025165604 28.719452107683917 48-49 20.866284015991415 25.482896322917064 25.967756779628676 27.683062881462845 50-51 21.7421754521436 25.65832152982453 24.834058753414787 27.765444264617084 52-53 21.549241395410437 25.181429844507946 24.07175036869597 29.197578391385647 54-55 20.325305450864107 25.06403076170231 23.83605431335005 30.774609474083537 56-57 20.787026332979412 24.52102979435988 24.922500544348093 29.76944332831261 58-59 22.506840797415872 23.152760225280534 24.495217614800815 29.845181362502775 60-61 22.590681251388922 23.456695900459945 23.914619665132022 30.038003183019107 62-63 21.97293311394894 23.314268141300907 24.394541728303057 30.3182570164471 64-65 21.90147065590827 23.396369995162537 24.15689540418626 30.545263944742935 66-67 23.12955328813202 25.08482261607202 22.590706606343375 29.19491748945259 68-69 22.832414060939115 25.339704261428047 22.68582434825944 29.14205732937339 70-71 22.507041273255286 25.143828319782134 23.338747826438365 29.01038258052421 72-73 23.88543824875586 24.638879972344995 23.6960957509613 27.779586027937842 74-75 24.72676414894202 24.139827611946163 23.840690899172408 27.292717339939408 76-77 25.056062241995836 23.870617997831587 23.709895013771444 27.363424746401126 78-79 25.46878735399324 24.120592298946427 24.570439600030976 25.840180747029358 80-81 26.149448186304603 24.655347929393887 24.221102594809484 24.974101289492026 82-83 25.127331987995216 24.502400732678023 24.81385623438514 25.556411044941623 84-85 25.25002385456015 24.408996256237266 24.95748275455622 25.383497134646365 86-87 26.007715351349287 24.008727752704694 24.60269981177416 25.380857084171858 88-89 26.410934546287272 23.390710664684992 24.816013865751863 25.382340923275876 90-91 27.260381288875816 23.091479250890597 24.491962764512703 25.156176695720884 92-93 27.541310787556867 23.738170258477957 24.16909781348324 24.55142114048193 94-95 26.851685554697465 24.181805528116527 23.61752186641283 25.348987050773182 96-97 26.793755696333243 24.432050320790548 22.81126111767109 25.96293286520511 98-99 26.55847515744412 25.03414890381177 22.818763321439675 25.588612617304427 100-101 26.04388685179012 26.350966864315755 22.800466456334817 24.80467982755931 102-103 25.51578153444866 27.81976659356348 23.00157804120865 23.662873830779212 104-105 24.782738011728725 29.408569264008527 22.88717416313589 22.921518561126856 106-107 24.0537300835492 30.589962423061557 22.45704691056509 22.89926058282415 108-109 23.912487177012228 32.21718156901742 22.062048670113064 21.808282583857288 110-111 23.697554821780113 34.2993878636734 21.044178419669173 20.958878894877316 112-113 23.326460681660794 35.89656677939013 20.567560560332492 20.209411978616586 114-115 22.73919094868226 36.907915413280655 20.631706550532034 19.72118708750505 116-117 22.31154978012925 37.99285728716085 20.1561654720992 19.5394274606107 118-119 22.40436974336856 38.724188591761916 19.9898012364316 18.881640428437926 120-121 22.85480511084573 38.825300123012276 19.421236228461627 18.898658537680365 122-123 23.365834987973226 39.15609436891155 19.10747096569985 18.370599677415374 124-125 23.61736790232355 40.964118396241275 18.113279298659336 17.30523440277584 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 2.0 2 4.0 3 6.0 4 7.0 5 8.5 6 7.5 7 7.0 8 11.0 9 12.5 10 16.0 11 21.0 12 25.5 13 36.0 14 51.0 15 66.5 16 87.0 17 119.0 18 158.0 19 216.5 20 294.5 21 381.0 22 526.5 23 701.0 24 898.5 25 1166.0 26 1457.0 27 1732.0 28 1971.0 29 2199.5 30 2404.0 31 2504.5 32 2485.0 33 2403.5 34 2360.0 35 2278.0 36 2118.0 37 1937.0 38 1780.0 39 1692.0 40 1576.0 41 1459.0 42 1418.0 43 1488.5 44 1710.0 45 2133.0 46 2712.5 47 3759.5 48 5942.5 49 10299.0 50 17638.0 51 28219.0 52 39550.0 53 46986.5 54 47837.5 55 43340.5 56 36419.5 57 28530.5 58 21663.0 59 16371.5 60 12105.0 61 9032.5 62 6924.0 63 5136.5 64 3880.5 65 3295.0 66 2696.5 67 2070.0 68 1576.5 69 1202.0 70 910.5 71 748.5 72 577.5 73 443.0 74 363.0 75 289.0 76 245.0 77 190.0 78 137.5 79 113.0 80 88.0 81 61.5 82 52.0 83 38.5 84 22.0 85 20.5 86 14.0 87 11.0 88 10.5 89 7.0 90 6.0 91 4.0 92 2.5 93 2.0 94 1.0 95 1.0 96 0.5 97 1.0 98 1.0 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14231552294220506 2 0.019080156861336642 3 0.00942784221383693 4 0.01503965305540653 5 0.0033670865049417603 6 0.0 7 4.489448673255681E-4 8 2.2447243366278404E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.734173009883521E-4 28-29 0.007183117877209089 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.489448673255681E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0016835432524708802 66-67 1.1223621683139202E-4 68-69 0.006285228142557954 70-71 0.01784555847619133 72-73 0.0 74-75 0.009091133563342754 76-77 4.489448673255681E-4 78-79 0.0010101259514825284 80-81 7.856535178197442E-4 82-83 0.0 84-85 0.0182945033435169 86-87 0.003030377854447584 88-89 0.0168354325247088 90-91 0.0 92-93 0.01223374763462173 94-95 0.01279492871877869 96-97 0.006060755708895168 98-99 0.003030377854447584 100-101 5.6118108415696E-4 102-103 0.0 104-105 3.3670865049417606E-4 106-107 6.734173009883521E-4 108-109 0.0 110-111 0.0 112-113 7.856535178197442E-4 114-115 0.006509700576220736 116-117 0.0 118-119 0.07564721014435823 120-121 0.00112236216831392 122-123 0.005499574624738209 124-125 0.2431036456567951 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 445489.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.80836596016991 #Duplication Level Percentage of deduplicated Percentage of total 1 85.23628821878168 56.092608481881044 2 7.599042590434204 10.001611514764232 3 2.5665749726955243 5.067063152020804 4 1.3120769844430438 3.4538256944057597 5 0.7887405163723511 2.595286227452253 6 0.5329207077345428 2.104238457740853 7 0.3791001943679436 1.7463575028576026 8 0.2802026705524635 1.4751743909386754 9 0.1996736080035969 1.1826174481279397 >10 1.0590388624346168 11.7050171860201 >50 0.02623699941195319 1.1453954030962212 >100 0.018059233266225532 2.15647367763784 >500 0.0010222207509184263 0.5013907812413654 >1k 0.0010222207509184263 0.7729400818152891 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1194 0.2680200857933641 No Hit ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1192 0.26757114092603856 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 1061 0.23816525211621387 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 791 0.17755769502726218 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 772 0.17329271878766928 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 673 0.15106994785505365 No Hit GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 495 0.1111138546630781 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.2447243366278404E-4 0.0 0.0 0.0 0.0 3 2.2447243366278404E-4 0.0 0.0 0.0 0.0 4 2.2447243366278404E-4 0.0 0.0 0.0 0.0 5 2.2447243366278404E-4 0.0 0.0 2.2447243366278404E-4 0.0 6 2.2447243366278404E-4 0.0 0.0 2.2447243366278404E-4 0.0 7 2.2447243366278404E-4 0.0 0.0 2.2447243366278404E-4 0.0 8 2.2447243366278404E-4 0.0 0.0 2.2447243366278404E-4 0.0 9 2.2447243366278404E-4 0.0 0.0 2.2447243366278404E-4 0.0 10-11 5.6118108415696E-4 0.0 0.0 5.6118108415696E-4 0.0 12-13 0.0012345983851453123 0.0 0.0 6.734173009883521E-4 0.0 14-15 0.0013468346019767043 0.0 0.0 7.856535178197442E-4 0.0 16-17 0.002132488119796448 0.0 0.0 0.0015713070356394882 0.0 18-19 0.0023569605534592324 0.0 0.0 0.0030303778544475846 0.0 20-21 0.0025814329871220168 0.0 0.0 0.005948519492063777 0.0 22-23 0.0026936692039534085 0.0 0.0 0.0211004087643017 0.0 24-25 0.0026936692039534085 0.0 0.0 0.05005735270680084 0.0 26-27 0.0033670865049417608 0.0 0.0 0.12604127150165323 0.0 28-29 0.00392826758909872 0.0 0.0 0.3866537669841455 0.0 30-31 0.004713921106918465 0.0 0.0 0.9331319067361933 0.0 32-33 0.004713921106918465 0.0 0.0 1.7443752819934948 0.0 34-35 0.004713921106918465 0.0 0.0 2.9740352735982256 0.0 36-37 0.004938393540581249 0.0 0.0 4.642987817881025 0.0 38-39 0.005275102191075425 0.0 0.0 6.825308817950612 0.0 40-41 0.005948519492063777 0.0 0.0 9.363306389158879 0.0 42-43 0.006846409226714914 0.0 0.0 11.376936355330884 0.0 44-45 0.006958645443546305 0.0 0.0 13.190898091759841 0.0 46-47 0.006958645443546305 0.0 0.0 15.343252021935447 0.0 48-49 0.006958645443546305 0.0 0.0 17.655991505963108 0.0 50-51 0.006958645443546305 0.0 0.0 19.86502472563857 0.0 52-53 0.007070881660377697 0.0 0.0 21.81737371742063 0.0 54-55 0.007183117877209089 0.0 0.0 23.729542143577056 0.0 56-57 0.0072953540940404816 0.0 0.0 25.70770546523034 0.0 58-59 0.007407590310871874 0.0 0.0 27.608986978354125 0.0 60-61 0.007407590310871874 0.0 0.0 29.55359167117482 0.0 62-63 0.007407590310871874 0.0 0.0 31.41940653977988 0.0 64-65 0.007407590310871874 0.0 0.0 33.21597166259997 0.0 66-67 0.007407590310871874 0.0 0.0 35.16798394573155 0.0 68-69 0.007407590310871874 0.0 0.0 37.221906713746016 0.0 70-71 0.007407590310871874 0.0 0.0 39.43834752373235 0.0 72-73 0.007407590310871874 0.0 0.0 41.60619005183069 0.0 74-75 0.007407590310871874 0.0 0.0 43.50388000601586 0.0 76-77 0.007407590310871874 0.0 0.0 45.47710493412856 0.0 78-79 0.007407590310871874 0.0 0.0 47.411271658783946 0.0 80-81 0.007407590310871874 1.1223621683139202E-4 0.0 49.32703164387897 0.0 82-83 0.007407590310871874 2.2447243366278404E-4 0.0 51.35311983011926 0.0 84-85 0.007407590310871874 2.2447243366278404E-4 0.0 53.46742568278903 0.0 86-87 0.007407590310871874 2.2447243366278404E-4 0.0 55.17790562729944 0.0 88-89 0.007407590310871874 2.2447243366278404E-4 0.0 56.70790973514498 0.0 90-91 0.007407590310871874 2.2447243366278404E-4 0.0 58.47787487457603 0.0 92-93 0.007407590310871874 2.2447243366278404E-4 0.0 60.057150681610544 0.0 94-95 0.007407590310871874 2.2447243366278404E-4 0.0 61.51307888634736 0.0 96-97 0.007407590310871874 2.2447243366278404E-4 0.0 62.93253032061398 0.0 98-99 0.007407590310871874 2.2447243366278404E-4 0.0 64.18688227992162 0.0 100-101 0.007407590310871874 2.2447243366278404E-4 0.0 65.3709743674928 0.0 102-103 0.007407590310871874 2.2447243366278404E-4 0.0 66.49378548067405 0.0 104-105 0.007407590310871874 2.2447243366278404E-4 0.0 67.57282446929105 0.0 106-107 0.007407590310871874 2.2447243366278404E-4 0.0 68.56072764984097 0.0 108-109 0.007407590310871874 2.2447243366278404E-4 0.0 69.4857785489653 0.0 110-111 0.007407590310871874 2.2447243366278404E-4 0.0 70.37805647277486 0.0 112-113 0.007407590310871874 2.2447243366278404E-4 0.0 71.27246688470422 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATTGTG 50 7.4553154E-6 59.48547 9 TATAAGG 100 6.91216E-11 53.54293 2 GGTACCG 35 0.0078006666 50.98754 8 GTATTCG 50 5.285469E-4 47.679356 1 ATAAGGG 115 2.746674E-10 46.559067 3 GTATAAG 130 1.8189894E-11 45.84553 1 GTACAAG 150 1.8189894E-12 43.706074 1 GTATAGG 205 0.0 43.60916 1 TATTCGG 55 8.528324E-4 43.26701 2 TACTAGG 110 9.618816E-9 43.26701 2 ACTAGGG 130 9.1495167E-10 41.18687 3 GTACTAG 135 1.3005774E-9 39.73279 1 GTCTGTA 60 0.0013084994 39.661427 2 TCTGTAC 60 0.0013084994 39.661427 3 AGTTAGA 80 1.1972223E-4 37.18259 2 ACAACAC 455 0.0 36.606438 5 GTATTGG 165 2.3101165E-10 36.120724 1 GGGATTG 100 1.0625066E-5 35.69128 7 GTCTAGG 120 9.1514084E-7 34.766193 1 TGTAAGG 120 9.281048E-7 34.703747 2 >>END_MODULE