FastQCFastQC Report
Thu 26 May 2016
SRR935922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445489
Sequences flagged as poor quality0
Sequence length125
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT82761.8577338609932006No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT45601.0235942975022954RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT26690.5991169254459706RNA PCR Primer, Index 39 (96% over 25bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA11400.25589857437557384No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT10470.2350226380449349No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT10410.23367580344295819No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT10020.22492137853010963RNA PCR Primer, Index 39 (96% over 28bp)
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC9120.20471885950045904No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG8450.1896792064450525RNA PCR Primer, Index 39 (96% over 30bp)
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA7900.1773332225935994No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC7860.17643533285894827No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC7490.16812985281342524No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG7150.1604977900688906Illumina PCR Primer Index 11 (96% over 25bp)
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA5280.11852144497394998No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT5210.1169501379383105RNA PCR Primer, Index 39 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTG304.2559586E-579.3535465
GTATTAG401.7393028E-459.7163121
GTCCAAG701.301305E-859.7163121
CGGAGAT603.0707452E-759.7163121
TATTAGG401.756816E-459.5954552
CTATACA453.1125353E-453.081171
TGTAAGG1350.052.973742
GTCTATG708.9027344E-751.185411
CAGTCCG350.007795665450.9958159
GTTCTAG558.371827E-443.4300461
GTGTAAG1650.043.4300421
ATTAGGG600.001297382539.7303053
GGTAGGG1053.21701E-739.7303053
TGGTAGG1104.632875E-737.924382
GTACGAG1304.060894E-836.74851
CTATGGG650.001922434336.674133
TTATAGG650.001922434336.674132
CTCATAA650.001922434336.674132
CTTTACA1156.459104E-736.349061
TACGAGG1355.7627403E-835.3158262
Produced by FastQC (version 0.11.5)