##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935922_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 445489 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.325691543450006 33.0 31.0 34.0 30.0 34.0 2 32.433604421209054 34.0 31.0 34.0 31.0 34.0 3 32.42004179676715 34.0 31.0 34.0 30.0 34.0 4 35.906359079573235 37.0 35.0 37.0 35.0 37.0 5 35.89881680580216 37.0 35.0 37.0 35.0 37.0 6 35.995510551326745 37.0 35.0 37.0 35.0 37.0 7 35.97277822796971 37.0 35.0 37.0 35.0 37.0 8 36.00005611810842 37.0 35.0 37.0 35.0 37.0 9 37.682207641490585 39.0 37.0 39.0 35.0 39.0 10-11 37.59382723254671 39.0 37.0 39.0 35.0 39.0 12-13 37.56796464110225 39.0 37.0 39.0 35.0 39.0 14-15 38.72667114115051 40.0 38.0 41.0 34.0 41.0 16-17 38.646828541221 40.0 38.0 41.0 34.5 41.0 18-19 38.697552577055774 40.0 38.0 41.0 34.0 41.0 20-21 38.79148306692197 40.0 38.0 41.0 35.0 41.0 22-23 38.6102024965824 40.0 38.0 41.0 34.0 41.0 24-25 38.28257375602989 40.0 38.0 41.0 33.0 41.0 26-27 38.11299830074368 40.0 37.0 41.0 33.0 41.0 28-29 38.041903391554 40.0 37.0 41.0 33.0 41.0 30-31 37.87209336257461 40.0 37.0 41.0 33.0 41.0 32-33 37.52178617204914 40.0 35.5 41.0 33.0 41.0 34-35 37.33540671037893 40.0 35.0 41.0 33.0 41.0 36-37 37.25314317525236 40.0 35.0 41.0 33.0 41.0 38-39 36.8215298245299 39.5 35.0 41.0 31.5 41.0 40-41 36.5344935565188 39.0 35.0 41.0 31.0 41.0 42-43 36.19470177714825 39.0 35.0 41.0 30.5 41.0 44-45 35.73517303457549 39.0 35.0 41.0 29.5 41.0 46-47 35.18779812745096 38.0 35.0 40.5 28.0 41.0 48-49 34.49341622352067 37.0 35.0 40.0 26.0 41.0 50-51 33.75766629479067 37.0 34.0 39.5 22.5 41.0 52-53 33.28661313747365 36.0 34.0 39.0 21.5 41.0 54-55 32.795748043161566 35.0 34.0 39.0 20.0 41.0 56-57 32.4122649493029 35.0 33.0 37.5 19.5 40.0 58-59 32.10822826152834 35.0 33.0 37.0 18.0 39.0 60-61 31.650729872118056 35.0 33.0 36.0 15.5 39.0 62-63 31.25571562934214 35.0 33.0 35.5 10.0 38.0 64-65 30.931000540978566 35.0 33.0 35.0 4.5 37.0 66-67 30.47215980641497 35.0 33.0 35.0 2.0 36.5 68-69 29.971327013686086 35.0 32.0 35.0 2.0 36.0 70-71 29.466470552583793 35.0 31.5 35.0 2.0 35.5 72-73 29.17976089196366 35.0 31.0 35.0 2.0 35.0 74-75 29.10587915751006 35.0 31.0 35.0 2.0 35.0 76-77 29.013389780667985 35.0 31.0 35.0 2.0 35.0 78-79 29.08228934945644 35.0 31.0 35.0 2.0 35.0 80-81 29.0660251992754 35.0 31.0 35.0 2.0 35.0 82-83 28.993737219100808 35.0 31.0 35.0 2.0 35.0 84-85 28.94413105598567 35.0 31.0 35.0 2.0 35.0 86-87 28.904452186249266 35.0 31.0 35.0 2.0 35.0 88-89 28.821053942970536 35.0 31.0 35.0 2.0 35.0 90-91 28.716110835508847 35.0 31.0 35.0 2.0 35.0 92-93 28.63037695655785 35.0 31.0 35.0 2.0 35.0 94-95 28.59738063117159 35.0 31.0 35.0 2.0 35.0 96-97 28.56572103912779 35.0 30.5 35.0 2.0 35.0 98-99 28.47778396324039 35.0 30.0 35.0 2.0 35.0 100-101 28.36911573574207 35.0 30.0 35.0 2.0 35.0 102-103 27.62089973040861 33.5 28.0 35.0 2.0 35.0 104-105 28.028487796556142 34.5 29.0 35.0 2.0 35.0 106-107 27.8805593404102 35.0 29.0 35.0 2.0 35.0 108-109 27.55897900958273 35.0 29.0 35.0 2.0 35.0 110-111 27.10305866138109 34.5 27.0 35.0 2.0 35.0 112-113 26.51258729171764 34.0 24.5 35.0 2.0 35.0 114-115 25.771222185059564 34.0 13.5 35.0 2.0 35.0 116-117 25.020863590346785 34.0 2.0 35.0 2.0 35.0 118-119 24.386168906527434 34.0 2.0 35.0 2.0 35.0 120-121 23.68285075501303 34.0 2.0 35.0 2.0 35.0 122-123 22.908409635254742 33.0 2.0 35.0 2.0 35.0 124-125 20.672067099299873 29.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20039373885209955 1101 2 0.26259122497933873 1101 3 0.14673321182896615 1101 4 0.2672434899645353 1101 5 0.2440231906973267 1101 6 0.21923932401338675 1101 7 0.2517222477358203 1101 8 0.2681428784140607 1101 9 0.2660833479066227 1101 10-11 0.32292041840067043 1101 12-13 0.37299209630249663 1101 14-15 0.5434542519412204 1101 16-17 0.3662113600247707 1101 18-19 0.3214559451426382 1101 20-21 0.4551179014942548 1101 22-23 0.4537653882449604 1101 24-25 0.3989763680904943 1101 26-27 0.3695964296732086 1101 28-29 0.5704974678529027 1101 30-31 0.6098069557670698 1101 32-33 0.48629839959057364 1101 34-35 0.5497954137505019 1101 36-37 0.7110142339114347 1101 38-39 0.7084991939396375 1101 40-41 0.6901784081475384 1101 42-43 0.46727782768983417 1101 44-45 0.5240976324493616 1101 46-47 0.6332563721722053 1101 48-49 0.8488806902786195 1101 50-51 0.8711237408600851 1101 52-53 1.0062606024323415 1101 54-55 0.8218659495179352 1101 56-57 0.7656212372451208 1101 58-59 0.8889681247341201 1101 60-61 0.803684598999233 1101 62-63 0.8711382690567255 1101 64-65 0.6713789069505793 1101 66-67 0.09402645387461206 1101 68-69 0.2241732130031444 1101 70-71 0.4498300896216172 1101 72-73 0.5230831143324757 1101 74-75 0.6668749070840363 1101 76-77 0.6113747569189307 1101 78-79 0.625795213488054 1101 80-81 0.8012644268268012 1101 82-83 0.7314374054520911 1101 84-85 0.7015859571780432 1101 86-87 0.6907118857769703 1101 88-89 0.5504711431836427 1101 90-91 0.5998021683960069 1101 92-93 0.5454661781394954 1101 94-95 0.5687315412577618 1101 96-97 0.6974446205838838 1101 98-99 0.6661878090843238 1101 100-101 0.5841553562697754 1101 102-103 0.7095574067324932 1101 104-105 0.7928558942725168 1101 106-107 1.0139676478060586 1101 108-109 0.8530192226370303 1101 110-111 1.044852230698467 1101 112-113 1.1852410589510143 1101 114-115 0.8526041762165306 1101 116-117 0.6493714987839283 1101 118-119 0.12625256839909582 1101 120-121 0.07274954152349267 1101 122-123 0.07805840342347281 1101 124-125 0.10437873810999321 1102 1 -0.046883119857149325 1102 2 0.014653117833049123 1102 3 0.18729178641778788 1102 4 0.19243825768599976 1102 5 0.0652095906203769 1102 6 0.2324041792600937 1102 7 0.21837729246026072 1102 8 0.22436823188140664 1102 9 0.1673228881586155 1102 10-11 0.38776484467747707 1102 12-13 0.34033544053641407 1102 14-15 0.45525272746299805 1102 16-17 0.3454661931497114 1102 18-19 0.32998905565528247 1102 20-21 0.2311861182875603 1102 22-23 0.44582138997623133 1102 24-25 0.5635160189513186 1102 26-27 0.4327035759654123 1102 28-29 0.5525175217145915 1102 30-31 0.5449174443695526 1102 32-33 0.631779067090811 1102 34-35 0.33305320445936815 1102 36-37 0.4559015812231664 1102 38-39 0.32886744920558186 1102 40-41 0.35326331244685605 1102 42-43 0.2813107217939006 1102 44-45 0.24871170381609886 1102 46-47 0.17314768697455207 1102 48-49 0.42853407587061554 1102 50-51 0.5480167147948762 1102 52-53 0.5525389760016353 1102 54-55 0.3935573027929138 1102 56-57 0.423954169821819 1102 58-59 0.5267572706415962 1102 60-61 0.42196090466433134 1102 62-63 0.7948121653729707 1102 64-65 0.648229200304435 1102 66-67 0.7975226547017726 1102 68-69 0.9538698803110215 1102 70-71 0.7663396596899084 1102 72-73 0.7380609925665809 1102 74-75 0.7319056851931052 1102 76-77 0.8555677933717512 1102 78-79 0.9217970649836751 1102 80-81 0.8926760118994217 1102 82-83 0.8431823799639702 1102 84-85 0.9056772815985497 1102 86-87 0.7291755050902324 1102 88-89 0.811117243020135 1102 90-91 1.0969708228155497 1102 92-93 0.9284071707335109 1102 94-95 0.9429109920608774 1102 96-97 0.8666761296730456 1102 98-99 0.9429924161825234 1102 100-101 0.9757887601362754 1102 102-103 0.9734586522840942 1102 104-105 0.7673888124219843 1102 106-107 0.8600169889621618 1102 108-109 0.7748326063053952 1102 110-111 0.9205856153286511 1102 112-113 1.0382695767926968 1102 114-115 0.8471789333580162 1102 116-117 0.6230327712663737 1102 118-119 0.36995567987355926 1102 120-121 0.016723764857147216 1102 122-123 -0.1938108857945693 1102 124-125 -0.13101668845536096 1103 1 0.11079706032302994 1103 2 0.028279243959175915 1103 3 0.20042241704842212 1103 4 0.19775357300131446 1103 5 0.11543481584560311 1103 6 0.2240258008817122 1103 7 0.22204846363143105 1103 8 0.20765652016969227 1103 9 0.22554360887934166 1103 10-11 0.23168376359639353 1103 12-13 0.09356742251839734 1103 14-15 0.21627750223778008 1103 16-17 0.41350673369025515 1103 18-19 0.5073426592588888 1103 20-21 0.3161973795488251 1103 22-23 0.2303371557419993 1103 24-25 0.296725478410778 1103 26-27 0.46657744983928495 1103 28-29 0.5165265307236027 1103 30-31 0.38265393085603705 1103 32-33 0.28045023826198445 1103 34-35 0.5027491504053074 1103 36-37 0.32095788752946675 1103 38-39 0.6479327645208954 1103 40-41 0.5208871863207278 1103 42-43 0.4341936046767856 1103 44-45 0.5289819740863777 1103 46-47 0.8087783176051886 1103 48-49 1.0740408326273752 1103 50-51 1.0059784265065872 1103 52-53 1.0994871741998367 1103 54-55 0.9749086541442651 1103 56-57 0.9725577734254287 1103 58-59 1.20550727064159 1103 60-61 1.0793370307904624 1103 62-63 1.2358031563639607 1103 64-65 1.2037246957999272 1103 66-67 1.2305181501972697 1103 68-69 0.9735207812119206 1103 70-71 1.1172856056358533 1103 72-73 1.0703132448188342 1103 74-75 1.0959822617696808 1103 76-77 1.1092840095879701 1103 78-79 0.9743984163350312 1103 80-81 1.160953038926447 1103 82-83 1.3615382358198254 1103 84-85 1.2131547590760299 1103 86-87 1.094727306892036 1103 88-89 0.8472208466237383 1103 90-91 0.9597636156083418 1103 92-93 0.951335098661442 1103 94-95 0.9958727037725907 1103 96-97 1.142779733276651 1103 98-99 1.090132055822167 1103 100-101 1.099887859235377 1103 102-103 1.0872086522840974 1103 104-105 1.1703054790886505 1103 106-107 1.0260079799531532 1103 108-109 0.8668033270261191 1103 110-111 0.8718243540673889 1103 112-113 0.9450038110269325 1103 114-115 1.0330460504751322 1103 116-117 1.196287275770878 1103 118-119 1.2244939681618483 1103 120-121 1.4480075486409305 1103 122-123 1.1998377628540808 1103 124-125 1.0634089872203134 1104 1 0.03515933256311854 1104 2 0.13146788275874854 1104 3 0.10014374609450982 1104 4 0.0701865847912373 1104 5 0.14801788122609594 1104 6 0.22982965940261835 1104 7 0.13358651293475532 1104 8 0.1848162200625154 1104 9 0.30799269783967986 1104 10-11 0.3386371398021879 1104 12-13 0.34378714384744313 1104 14-15 0.47041469409200687 1104 16-17 0.37575753754877894 1104 18-19 0.31277942238858003 1104 20-21 0.43480402477926106 1104 22-23 0.5479684526337465 1104 24-25 0.6711868932017779 1104 26-27 0.5339354348339285 1104 28-29 0.5627993067150285 1104 30-31 0.6350011977370684 1104 32-33 0.446505972452762 1104 34-35 0.38561088673971966 1104 36-37 0.2720216603054624 1104 38-39 0.5260490560535871 1104 40-41 0.24016639318032418 1104 42-43 0.3970446228975746 1104 44-45 0.48863631492238824 1104 46-47 0.3964900003490186 1104 48-49 0.3027546589939334 1104 50-51 0.14192965908965505 1104 52-53 0.11605737784399395 1104 54-55 0.24531058340471645 1104 56-57 0.2117083629216765 1104 58-59 0.04594939282809207 1104 60-61 0.0658054123553029 1104 62-63 0.007199190661925314 1104 64-65 0.09998139462094002 1104 66-67 0.014347196285157793 1104 68-69 -0.36029486723395365 1104 70-71 -0.37211953905868356 1104 72-73 -0.5146438827267197 1104 74-75 -0.4721983384446844 1104 76-77 -0.5122192058461152 1104 78-79 -0.3429086576199545 1104 80-81 -0.6533476041603699 1104 82-83 -0.6482956334191883 1104 84-85 -0.4155456696485196 1104 86-87 -0.5198332504498708 1104 88-89 -0.5257266346195628 1104 90-91 -0.5004427080786868 1104 92-93 -0.6452056301702846 1104 94-95 -0.7495506617150873 1104 96-97 -0.529452313883052 1104 98-99 -0.5930780192046292 1104 100-101 -0.6422209296177854 1104 102-103 -0.45851482038471403 1104 104-105 -0.44292067310856 1104 106-107 -0.5749030597038676 1104 108-109 -0.6527250759749528 1104 110-111 -0.7238482075617654 1104 112-113 -0.5487046562828226 1104 114-115 -0.4045075400929292 1104 116-117 -0.2962636352687795 1104 118-119 -0.26676273065916334 1104 120-121 -0.06122878576421442 1104 122-123 0.020352232307455864 1104 124-125 0.08555528947112023 1105 1 0.06401875091314224 1105 2 0.07249774475662463 1105 3 0.12444155580440253 1105 4 0.13414910729158436 1105 5 0.2049085000561348 1105 6 -0.017784406454808277 1105 7 0.027207953264607454 1105 8 0.06827852973907511 1105 9 0.20253722929400908 1105 10-11 0.04063511925827612 1105 12-13 0.02840793288522292 1105 14-15 0.06969058038769305 1105 16-17 0.3386582488417673 1105 18-19 0.183144094665586 1105 20-21 0.02599004302569341 1105 22-23 0.11095916531137817 1105 24-25 0.14015051828318548 1105 26-27 0.17519786451232733 1105 28-29 0.15572509531690315 1105 30-31 -0.013901093067410386 1105 32-33 0.2731456130626171 1105 34-35 0.26549237209590615 1105 36-37 0.23883268816742742 1105 38-39 0.46550453485581755 1105 40-41 0.4944318434180204 1105 42-43 0.7060596333849176 1105 44-45 0.6697993584563875 1105 46-47 0.6749904388173036 1105 48-49 0.8003725710643721 1105 50-51 0.684220053300848 1105 52-53 1.0061697898298192 1105 54-55 0.9189517163452265 1105 56-57 0.782641505482836 1105 58-59 0.75138845086488 1105 60-61 0.9838426129276243 1105 62-63 0.8714291531741658 1105 64-65 0.9317031646994494 1105 66-67 0.9014711007554794 1105 68-69 0.9925797923761941 1105 70-71 0.8745663073902357 1105 72-73 0.9303052703371755 1105 74-75 0.983322772136507 1105 76-77 1.0081875183599003 1105 78-79 0.9123529617895763 1105 80-81 0.9418820660396854 1105 82-83 0.7840661465056336 1105 84-85 0.9875207877841596 1105 86-87 1.1213381521870929 1105 88-89 1.0099162214243762 1105 90-91 1.0302141738220598 1105 92-93 1.0779260077523496 1105 94-95 1.035155000423309 1105 96-97 1.058397755605199 1105 98-99 1.0494382759178613 1105 100-101 1.084556918246541 1105 102-103 0.950382495984254 1105 104-105 1.0488381744634516 1105 106-107 0.9309521585815403 1105 108-109 0.8043152887486045 1105 110-111 0.9264694896335754 1105 112-113 1.018815613259786 1105 114-115 1.3177151094862936 1105 116-117 1.3315344847022992 1105 118-119 1.3437005072368073 1105 120-121 0.8882268468865462 1105 122-123 0.8047181456291987 1105 124-125 0.5675357814786892 1106 1 0.06391632637807731 1106 2 0.16658199625275216 1106 3 0.18623281460193652 1106 4 0.09108690633464533 1106 5 0.2915816048364306 1106 6 0.24813895072880854 1106 7 0.27047354008403346 1106 8 0.1712956639005796 1106 9 0.17072709511786854 1106 10-11 0.34548345778600265 1106 12-13 0.3134298078394906 1106 14-15 0.30370869489832586 1106 16-17 0.31662599974529826 1106 18-19 0.4239405185861074 1106 20-21 0.39965303704118327 1106 22-23 0.2934870028367982 1106 24-25 0.13794107474105743 1106 26-27 0.1899872357720085 1106 28-29 0.3935219435676345 1106 30-31 0.42121662198753285 1106 32-33 0.637239229087669 1106 34-35 0.46066963970194763 1106 36-37 0.3102774593949107 1106 38-39 0.1842248134505624 1106 40-41 0.3730660854033019 1106 42-43 0.24217525605293844 1106 44-45 0.15755231047781137 1106 46-47 0.03466516775809225 1106 48-49 0.3184139213123913 1106 50-51 0.45248454271454364 1106 52-53 0.44043518643225354 1106 54-55 0.4096640058262295 1106 56-57 0.3866938590523361 1106 58-59 0.43491858562630625 1106 60-61 0.26779268828281033 1106 62-63 0.3788841961193121 1106 64-65 0.2509494664421297 1106 66-67 0.18216188108411657 1106 68-69 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-1.183128415524017 2216 46-47 -1.1776069637801 2216 48-49 -1.5089461803596436 2216 50-51 -1.5723873743592094 2216 52-53 -1.2515084967958288 2216 54-55 -1.8255458913102771 2216 56-57 -1.6056132222455695 2216 58-59 -1.145239482605156 2216 60-61 -1.5084335319801028 2216 62-63 -1.6092726012117957 2216 64-65 -1.573537208961973 2216 66-67 -1.8461593389802218 2216 68-69 -1.792280084588949 2216 70-71 -1.9175845242342788 2216 72-73 -1.7355525560469687 2216 74-75 -1.4628380845506683 2216 76-77 -1.675293912489952 2216 78-79 -1.6259587265221143 2216 80-81 -1.6251833247099192 2216 82-83 -1.563894664613077 2216 84-85 -1.8865963231750555 2216 86-87 -2.102004294839567 2216 88-89 -2.1022163828134914 2216 90-91 -2.1852904969457683 2216 92-93 -2.5300826502563076 2216 94-95 -2.4812268633269774 2216 96-97 -2.3890276260307104 2216 98-99 -2.587119026428919 2216 100-101 -2.2373956905481727 2216 102-103 -2.3172069321314908 2216 104-105 -2.2101274213442466 2216 106-107 -2.4419573880122165 2216 108-109 -2.2528503526275614 2216 110-111 -2.080502485759453 2216 112-113 -1.70893558291246 2216 114-115 -1.5683067633776844 2216 116-117 -1.1437560142724124 2216 118-119 -1.0841532179853388 2216 120-121 -0.9728690747356943 2216 122-123 -1.3195128864965682 2216 124-125 -1.7188962075848764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 35.0 5 100.0 6 289.0 7 697.0 8 1006.0 9 1388.0 10 2021.0 11 3811.0 12 5365.0 13 5444.0 14 7109.0 15 9241.0 16 4588.0 17 2227.0 18 2300.0 19 3480.0 20 4391.0 21 6816.0 22 6898.0 23 2230.0 24 2835.0 25 3440.0 26 4677.0 27 5666.0 28 6939.0 29 8660.0 30 11079.0 31 14904.0 32 20303.0 33 27614.0 34 38532.0 35 56896.0 36 124839.0 37 46827.0 38 2827.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.091524142832725 20.225863914854568 11.637097282075736 16.045514660236975 2 19.25833641985701 21.156043413358475 37.28363469028138 22.301985476503138 3 29.13539952726105 22.414470390963636 28.40025230701544 20.04987777475987 4 15.899927633619354 22.701018972577184 27.26144939522382 34.13760399857964 5 15.529404896624813 33.50465233245316 27.74312478669325 23.222817984228772 6 31.112368176491977 35.651945533152855 9.391625250851886 23.84406103950328 7 27.28754245334902 40.00368134791207 11.94193347086011 20.766842727878803 8 35.858348915461434 27.42761325195459 12.589536441977241 24.124501390606724 9 33.65739670339784 14.232899128822485 17.138919255020888 34.97078491275879 10-11 29.239891445131082 25.226661039890995 18.121435097162895 27.412012417815028 12-13 32.90384274359187 25.78425056510935 19.49262495819201 21.819281733106767 14-15 35.34419480615683 19.525959114590933 23.066675046970857 22.06317103228138 16-17 30.633191840875977 21.389529258859366 24.52136865332253 23.455910246942125 18-19 29.44842633600381 21.542507222400552 24.601168603489647 24.407897838105992 20-21 35.497397242131676 18.926168771843972 22.914819445597985 22.661614540426363 22-23 30.93376042955045 22.34914891276777 22.88159752541589 23.835493132265892 24-25 26.38549997867512 23.497886592037066 24.421590656559424 25.695022772728393 26-27 23.55232115720029 22.408072926604248 27.553878995889907 26.485726920305552 28-29 24.183313168226377 19.186781267326467 30.207704342868176 26.422201221578984 30-31 25.373017066639132 20.26278987808902 28.040310759637162 26.323882295634682 32-33 22.679572335119385 20.34247759203931 29.451007768990927 27.526942303850376 34-35 21.983820026981586 21.738808365638658 29.783339207028682 26.494032400351074 36-37 23.818320991090687 22.236127042418556 26.21905366911416 27.72649829737659 38-39 24.9681810325283 18.057235981135335 28.63909097643264 28.335492009903724 40-41 23.304840299087072 18.391475434859224 29.91936950182833 28.384314764225376 42-43 24.788154140730747 18.05196087894426 31.363961848665173 25.795923131659816 44-45 24.044364731789113 18.50954793496585 31.099757794244077 26.346329539000962 46-47 22.611893896370056 18.09629418459266 29.68165319457944 29.61015872445784 48-49 21.83746400023345 18.98408265972897 30.30097263905506 28.877480700982517 50-51 21.834433622379006 19.617094922658023 29.43899849378997 29.109472961173005 52-53 21.298617923225937 19.998698059884756 28.98724772104362 29.715436295845688 54-55 19.60284092312044 20.567174498135756 27.172051386229512 32.65793319251429 56-57 20.42553239249454 21.117580905476903 26.480339581897645 31.97654712013091 58-59 20.191856589051582 21.087726071799754 26.754981604484062 31.965435734664606 60-61 21.129814653111527 22.78069716648447 23.760519339422522 32.32896884098148 62-63 19.16837452776612 23.898682122341967 24.04245671610298 32.89048663378894 64-65 18.310889831174283 23.578584431938836 24.142908130167076 33.967617606719806 66-67 19.048842956840687 24.374900390357563 23.53368994520628 33.04256670759547 68-69 18.787444807840373 23.72415480516915 24.04582380260792 33.44257658438256 70-71 18.116945648489637 22.859262518266444 24.9642527649392 34.059539068304716 72-73 18.173961646639984 21.257876176516145 25.76247673904406 34.80568543779981 74-75 17.775298604454882 20.509260610250756 26.514683864248052 35.20075692104631 76-77 17.15867282918321 20.51363782270718 27.16037881967905 35.167310528430555 78-79 17.087178359061618 21.202992666485592 27.535808964980056 34.17402000947274 80-81 17.13992938097237 21.912437793076823 26.65531584393779 34.29231698201302 82-83 16.226102103531176 21.34070650453771 27.305612484258873 35.127578907672245 84-85 16.368080917822887 20.268738397581085 27.8935057880217 35.469674896574325 86-87 16.74261317338924 20.25291309100786 27.716284801644935 35.28818893395796 88-89 16.947668741540195 20.447755163427157 27.53457436659491 35.07000172843774 90-91 17.051823950759726 19.748972477434908 27.503372698315783 35.695830873489584 92-93 16.85765529564142 19.56939453050468 27.509096745374183 36.06385342847972 94-95 16.40972055426733 19.345258805492392 27.7433337299013 36.501686910338975 96-97 16.546873211235294 19.45884185692576 27.60236504156107 36.391919890277876 98-99 16.929935419280838 19.935060124941355 27.67374727546584 35.461257180311975 100-101 17.18661964717423 20.67559468359488 27.086415152787165 35.05137051644373 102-103 16.80950595862075 21.710300366563484 26.686629748433745 34.79356392638202 104-105 16.441707876064278 23.265445386979252 26.671028914294183 33.62181782266229 106-107 15.864925957767756 25.266055951998815 26.122081577771844 32.74693651246159 108-109 15.267829284224751 26.60256482202703 25.68335020617793 32.446255687570286 110-111 15.211528122751746 28.007035037133726 25.32433788380965 31.45709895630488 112-113 15.355390767662913 29.174378238719967 24.884144035143443 30.586086958473675 114-115 15.00457928904293 29.81487550619102 24.84488780741845 30.335657397347603 116-117 14.626223715785905 30.336927314481205 24.90442614155482 30.13242282817807 118-119 14.6463694135975 30.690784526318858 25.224645830667665 29.43820022941598 120-121 14.823536013917337 31.11422879429838 25.107438480316507 28.954796711467772 122-123 14.810720170244096 31.36743004143863 24.940288858260114 28.881560930057155 124-125 14.168600519361544 32.77669811352529 24.878607940416007 28.176093426697157 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.5 5 2.5 6 2.5 7 3.0 8 4.5 9 5.0 10 6.0 11 11.0 12 13.5 13 13.5 14 15.5 15 20.0 16 21.5 17 23.5 18 36.0 19 53.0 20 80.0 21 118.0 22 181.5 23 270.5 24 367.0 25 492.5 26 650.5 27 884.0 28 1185.5 29 1487.0 30 1779.0 31 2057.5 32 2365.0 33 2613.0 34 2777.0 35 2823.5 36 2858.5 37 2936.5 38 2834.5 39 2733.5 40 2769.0 41 2855.5 42 3025.5 43 3245.0 44 3576.5 45 4002.5 46 4422.5 47 5009.0 48 6178.0 49 8740.0 50 14105.0 51 23487.5 52 35437.0 53 44950.0 54 47726.5 55 45236.0 56 39890.5 57 32039.0 58 23637.0 59 16798.0 60 11955.5 61 8576.0 62 6387.0 63 4644.0 64 3409.5 65 2843.0 66 2265.5 67 1709.0 68 1308.0 69 981.5 70 691.5 71 531.0 72 375.0 73 263.0 74 211.5 75 161.5 76 112.5 77 70.5 78 41.5 79 33.0 80 26.0 81 16.0 82 11.5 83 4.5 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19865810379156387 2 8.978897346511362E-4 3 0.0 4 0.11919486227493833 5 0.027161164473196872 6 0.0015713070356394884 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0010101259514825284 112-113 1.1223621683139202E-4 114-115 0.00112236216831392 116-117 0.004938393540581249 118-119 0.00224472433662784 120-121 3.3670865049417606E-4 122-123 0.0024691967702906246 124-125 0.030191542327644455 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 445489.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.8772121957906 #Duplication Level Percentage of deduplicated Percentage of total 1 82.19593785333424 47.57271736769442 2 9.027474679923092 10.44970135284071 3 3.050640347677806 5.296876762067663 4 1.5810658438174408 3.660307333525549 5 0.9702422789892431 2.8077459131193945 6 0.6472664398279798 2.2477186251082752 7 0.4530300137187807 1.8354079964544658 8 0.35717748960948814 1.6537949886150507 9 0.26767769126866253 1.3943194683872135 >10 1.383572064048952 13.48423257119774 >50 0.03683501935581974 1.4173823567465664 >100 0.023264222742779526 2.4391274273540993 >500 0.0031018963657039366 1.3119090017768023 >1k 0.0023264222742779526 2.5715301490524083 >5k 3.8773704571299207E-4 1.8572286860596674 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 8276 1.8577338609932006 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 4560 1.0235942975022954 RNA PCR Primer, Index 39 (95% over 23bp) ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 2669 0.5991169254459706 RNA PCR Primer, Index 39 (96% over 25bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1140 0.25589857437557384 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 1047 0.2350226380449349 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1041 0.23367580344295819 No Hit CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT 1002 0.22492137853010963 RNA PCR Primer, Index 39 (96% over 28bp) ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 912 0.20471885950045904 No Hit TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 845 0.1896792064450525 RNA PCR Primer, Index 39 (96% over 30bp) GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 790 0.1773332225935994 No Hit TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC 786 0.17643533285894827 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 749 0.16812985281342524 No Hit CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG 715 0.1604977900688906 Illumina PCR Primer Index 11 (96% over 25bp) GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 528 0.11852144497394998 No Hit TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT 521 0.1169501379383105 RNA PCR Primer, Index 39 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.2447243366278404E-4 0.0 0.0 0.0 0.0 7 2.2447243366278404E-4 0.0 0.0 0.0 0.0 8 2.2447243366278404E-4 0.0 0.0 0.0 0.0 9 2.2447243366278404E-4 0.0 0.0 0.0 0.0 10-11 2.2447243366278404E-4 0.0 0.0 3.3670865049417606E-4 0.0 12-13 2.2447243366278404E-4 0.0 0.0 4.489448673255681E-4 0.0 14-15 4.489448673255681E-4 0.0 0.0 5.6118108415696E-4 0.0 16-17 4.489448673255681E-4 0.0 0.0 0.0013468346019767043 0.0 18-19 4.489448673255681E-4 0.0 0.0 0.002693669203953408 0.0 20-21 4.489448673255681E-4 0.0 0.0 0.0056118108415696005 0.0 22-23 5.6118108415696E-4 0.0 0.0 0.020202519029650562 0.0 24-25 8.978897346511362E-4 0.0 0.0 0.049608407839475274 0.0 26-27 0.00112236216831392 0.0 0.0 0.12435772824918236 0.0 28-29 0.0015713070356394884 0.0 0.0 0.3841845702138549 0.0 30-31 0.0019080156861336643 0.0 0.0 0.9304382375322399 0.0 32-33 0.0020202519029650563 0.0 0.0 1.7407837230548902 0.0 34-35 0.002132488119796448 0.0 0.0 2.970668187093284 0.0 36-37 0.0024691967702906246 0.0 0.0 4.639171786508758 0.0 38-39 0.0025814329871220168 0.0 0.0 6.822727384963489 0.0 40-41 0.0032548502881103686 0.0 0.0 9.362857444291553 0.0 42-43 0.0038160313722673286 0.0 0.0 11.3855785440269 0.0 44-45 0.0040405038059301126 0.0 0.0 13.203356311828125 0.0 46-47 0.0040405038059301126 0.0 0.0 15.360087454460155 0.0 48-49 0.0040405038059301126 0.0 0.0 17.682030308267993 0.0 50-51 0.004377212456424289 0.0 0.0 19.898583354471153 0.0 52-53 0.00448944867325568 0.0 0.0 21.857329810612608 0.0 54-55 0.00448944867325568 0.0 0.0 23.778701606549205 0.0 56-57 0.00448944867325568 0.0 0.0 25.76662947906682 0.0 58-59 0.00448944867325568 0.0 0.0 27.67879790522325 0.0 60-61 0.00448944867325568 0.0 0.0 29.649104691698334 0.0 62-63 0.00448944867325568 0.0 0.0 31.549937259954792 0.0 64-65 0.00448944867325568 0.0 0.0 33.370633169393635 0.0 66-67 0.00448944867325568 0.0 0.0 35.342735735338024 0.0 68-69 0.00448944867325568 0.0 0.0 37.430329368401914 0.0 70-71 0.00448944867325568 0.0 0.0 39.67393134286144 0.0 72-73 0.00448944867325568 0.0 0.0 41.858609303484485 0.0 74-75 0.00448944867325568 0.0 0.0 43.77044102099042 0.0 76-77 0.00448944867325568 0.0 0.0 45.76499083030109 0.0 78-79 0.00448944867325568 0.0 0.0 47.72384952265937 0.0 80-81 0.00448944867325568 0.0 0.0 49.66867868791373 0.0 82-83 0.00448944867325568 0.0 0.0 51.72529512513216 0.0 84-85 0.00448944867325568 0.0 0.0 53.86923133904541 0.0 86-87 0.00448944867325568 0.0 0.0 55.61383109347257 0.0 88-89 0.00448944867325568 0.0 0.0 57.169874003622986 0.0 90-91 0.00448944867325568 0.0 0.0 58.96632689022624 0.0 92-93 0.00448944867325568 0.0 0.0 60.57669212932306 0.0 94-95 0.00448944867325568 0.0 0.0 62.05484310499249 0.0 96-97 0.00448944867325568 0.0 0.0 63.489334192314516 0.0 98-99 0.00448944867325568 0.0 0.0 64.76186841874883 0.0 100-101 0.00448944867325568 0.0 0.0 65.9687444583368 0.0 102-103 0.00448944867325568 0.0 0.0 67.11063572837938 0.0 104-105 0.00448944867325568 0.0 0.0 68.20707133060525 0.0 106-107 0.00448944867325568 0.0 0.0 69.21270783341453 0.0 108-109 0.00448944867325568 0.0 0.0 70.15582876344871 0.0 110-111 0.00448944867325568 0.0 0.0 71.06830920628792 0.0 112-113 0.00448944867325568 0.0 0.0 71.98236095616278 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGGTG 30 4.2559586E-5 79.353546 5 GTATTAG 40 1.7393028E-4 59.716312 1 GTCCAAG 70 1.301305E-8 59.716312 1 CGGAGAT 60 3.0707452E-7 59.716312 1 TATTAGG 40 1.756816E-4 59.595455 2 CTATACA 45 3.1125353E-4 53.08117 1 TGTAAGG 135 0.0 52.97374 2 GTCTATG 70 8.9027344E-7 51.18541 1 CAGTCCG 35 0.0077956654 50.995815 9 GTTCTAG 55 8.371827E-4 43.430046 1 GTGTAAG 165 0.0 43.430042 1 ATTAGGG 60 0.0012973825 39.730305 3 GGTAGGG 105 3.21701E-7 39.730305 3 TGGTAGG 110 4.632875E-7 37.92438 2 GTACGAG 130 4.060894E-8 36.7485 1 CTATGGG 65 0.0019224343 36.67413 3 TTATAGG 65 0.0019224343 36.67413 2 CTCATAA 65 0.0019224343 36.67413 2 CTTTACA 115 6.459104E-7 36.34906 1 TACGAGG 135 5.7627403E-8 35.315826 2 >>END_MODULE