##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935921_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1266369 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.125342613408886 31.0 31.0 34.0 28.0 34.0 2 31.08509447088487 33.0 31.0 34.0 28.0 34.0 3 31.288025843968068 34.0 31.0 34.0 28.0 34.0 4 34.51607390894755 37.0 35.0 37.0 32.0 37.0 5 34.6298093209799 37.0 35.0 37.0 32.0 37.0 6 34.733491581047865 37.0 35.0 37.0 32.0 37.0 7 34.72225709883928 37.0 35.0 37.0 32.0 37.0 8 34.63962952346433 37.0 35.0 37.0 32.0 37.0 9 36.24730311623232 39.0 38.0 39.0 32.0 39.0 10-11 36.009027384593274 39.0 37.0 39.0 31.0 39.0 12-13 35.83126600540601 39.0 37.0 39.0 30.0 39.0 14-15 37.31929635043183 40.0 38.0 41.0 31.0 41.0 16-17 37.494851421663036 40.5 38.0 41.0 31.0 41.0 18-19 37.560197699090864 40.0 38.0 41.0 31.0 41.0 20-21 37.688567866080106 40.5 38.0 41.0 31.0 41.0 22-23 37.62294836655035 40.5 38.0 41.0 31.0 41.0 24-25 37.591935289003445 40.0 38.0 41.0 31.0 41.0 26-27 37.55869537235988 40.0 38.0 41.0 31.0 41.0 28-29 37.4622799515781 40.0 37.0 41.0 31.0 41.0 30-31 37.377290900203654 40.0 37.0 41.0 30.0 41.0 32-33 37.28962845742434 40.0 37.0 41.0 30.0 41.0 34-35 37.04348456097709 40.0 36.0 41.0 30.0 41.0 36-37 37.04844085728567 40.0 36.5 41.0 30.0 41.0 38-39 37.170952937098114 40.0 36.5 41.0 30.0 41.0 40-41 37.148207986771624 40.0 36.0 41.0 30.0 41.0 42-43 36.999876813156355 40.0 36.0 41.0 30.0 41.0 44-45 36.82887926031039 40.0 35.0 41.0 30.0 41.0 46-47 36.66671523071079 40.0 35.0 41.0 30.0 41.0 48-49 36.50167407761877 40.0 35.0 41.0 30.0 41.0 50-51 36.274915131371664 39.0 35.0 41.0 30.0 41.0 52-53 36.012584009873905 39.0 35.0 41.0 30.0 41.0 54-55 35.72919307089798 38.5 35.0 41.0 29.5 41.0 56-57 35.43225552741737 38.0 35.0 40.0 29.0 41.0 58-59 35.035760903812395 37.0 35.0 40.0 28.5 41.0 60-61 34.705110437794986 37.0 34.0 39.0 28.0 41.0 62-63 34.267031568208004 36.0 34.0 39.0 27.5 41.0 64-65 33.81811265120987 35.5 34.0 38.5 27.0 40.5 66-67 33.49065240857917 35.0 33.5 37.0 26.5 40.0 68-69 33.282604043529176 35.0 34.0 37.0 27.5 39.0 70-71 33.11598120295112 35.0 34.0 36.0 28.0 39.0 72-73 32.861363473047746 35.0 34.0 36.0 27.5 38.5 74-75 32.552863343938455 35.0 33.5 35.0 27.0 37.0 76-77 32.358934481182025 35.0 33.0 35.0 27.0 37.0 78-79 32.180514921006434 35.0 33.0 35.0 26.5 36.5 80-81 32.02064406188086 35.0 33.0 35.0 26.0 36.0 82-83 31.903974276060136 35.0 33.0 35.0 26.5 36.0 84-85 31.671744570500383 35.0 33.0 35.0 25.0 35.0 86-87 31.478010753579724 35.0 33.0 35.0 25.0 35.0 88-89 31.358980281418766 35.0 33.0 35.0 25.0 35.0 90-91 31.29881772216471 35.0 33.0 35.0 24.0 35.0 92-93 31.192490498425023 35.0 33.0 35.0 23.5 36.0 94-95 31.0197505624348 35.0 32.0 35.0 23.0 35.0 96-97 30.90711001295831 35.0 32.0 35.0 21.5 35.0 98-99 30.749135915361162 35.0 32.0 35.0 20.0 35.0 100-101 30.613946645882834 35.0 32.0 35.0 19.0 35.0 102-103 29.89489122048945 34.0 30.5 35.0 17.5 35.0 104-105 30.384618938082028 35.0 31.5 35.0 18.0 35.0 106-107 30.389248710289024 35.0 32.0 35.0 16.0 35.0 108-109 30.260781810041152 35.0 31.5 35.0 14.0 35.0 110-111 30.13613567609441 35.0 31.0 35.0 9.0 35.0 112-113 29.984134166265914 35.0 31.0 35.0 4.5 35.0 114-115 29.80602770598459 35.0 31.0 35.0 2.0 35.0 116-117 29.655909928306837 35.0 31.0 35.0 2.0 35.0 118-119 29.42624701015265 35.0 31.0 35.0 2.0 35.0 120-121 29.11485791266211 35.0 30.5 35.0 2.0 35.0 122-123 28.5516488480056 35.0 30.0 35.0 2.0 35.0 124-125 25.747897334821054 32.0 23.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1252367649574353 1101 2 0.2527478036022295 1101 3 0.2152758334788203 1101 4 0.28218172947654097 1101 5 0.20516461834038324 1101 6 0.14419469332723622 1101 7 0.13475158616904537 1101 8 0.10166889239673083 1101 9 0.09267767276618599 1101 10-11 0.3239522375159751 1101 12-13 0.3102029618057358 1101 14-15 0.28629686388532605 1101 16-17 0.2152768748886018 1101 18-19 0.3078134261662555 1101 20-21 0.24898173432837467 1101 22-23 0.2869822780274589 1101 24-25 0.2915042866157336 1101 26-27 0.34451992719640145 1101 28-29 0.24868887113361637 1101 30-31 0.12761771451145876 1101 32-33 0.15051428152031576 1101 34-35 0.08319032903720114 1101 36-37 0.0652602807241962 1101 38-39 0.21037769743739432 1101 40-41 0.17594816255790136 1101 42-43 0.0856665165920063 1101 44-45 0.25917271798427066 1101 46-47 0.17701053739636308 1101 48-49 0.12640043491203556 1101 50-51 0.2455668831394675 1101 52-53 0.31078100648404217 1101 54-55 0.14117945479629412 1101 56-57 -0.029196687373556074 1101 58-59 0.03264016517283608 1101 60-61 0.05030436461893828 1101 62-63 0.09151475521771602 1101 64-65 0.1341952139441034 1101 66-67 -0.045733155616680676 1101 68-69 0.05156370233806484 1101 70-71 -0.02294859589416376 1101 72-73 -0.0030931423159827887 1101 74-75 0.10300821932256099 1101 76-77 0.1783145302438669 1101 78-79 0.14197818301170173 1101 80-81 0.0814365476226655 1101 82-83 0.015548837831499185 1101 84-85 -0.07454657094430317 1101 86-87 -0.07849287759102808 1101 88-89 0.0756565570476333 1101 90-91 0.006882963300178346 1101 92-93 -0.38085483947005017 1101 94-95 -0.39277391469013523 1101 96-97 -0.34602757756620406 1101 98-99 -0.23015370083501452 1101 100-101 -0.1937371465977762 1101 102-103 -0.1830832655987642 1101 104-105 -0.16637182960451113 1101 106-107 -0.10308400413276075 1101 108-109 -0.12720568413902456 1101 110-111 -0.2924296332327856 1101 112-113 -0.19099748088897783 1101 114-115 -0.27734919578221806 1101 116-117 -0.35190030514081627 1101 118-119 -0.5392705447337214 1101 120-121 -0.5874471870610769 1101 122-123 -0.4933105801397666 1101 124-125 -0.10471716504161677 1102 1 0.3279618689039978 1102 2 0.3191229654862191 1102 3 0.16796549335204247 1102 4 0.37343999408017936 1102 5 0.2929323233366361 1102 6 0.21665396672373305 1102 7 0.31607664921860135 1102 8 0.16372124056658777 1102 9 0.18254134940717393 1102 10-11 0.3410318503576377 1102 12-13 0.4312100506204146 1102 14-15 0.3192533767337977 1102 16-17 0.35290484844186665 1102 18-19 0.3446128945051541 1102 20-21 0.32947590650477565 1102 22-23 0.4102642628955593 1102 24-25 0.3956945258632345 1102 26-27 0.40335674813567834 1102 28-29 0.42691734908331114 1102 30-31 0.31635808667422793 1102 32-33 0.42492604622619723 1102 34-35 0.41023586103911214 1102 36-37 0.3442010482247042 1102 38-39 0.28381236446758606 1102 40-41 0.331193544604119 1102 42-43 0.19620260820129687 1102 44-45 0.18211730253884184 1102 46-47 0.22856735015622576 1102 48-49 0.3066925077087106 1102 50-51 0.2882889198104479 1102 52-53 0.295418386349084 1102 54-55 0.43414653270509973 1102 56-57 0.4043323835827479 1102 58-59 0.36434761524439807 1102 60-61 0.3484384386425248 1102 62-63 0.2975102565468717 1102 64-65 0.2478527696662809 1102 66-67 0.2248533756354476 1102 68-69 0.3121400093382647 1102 70-71 0.35916339374526274 1102 72-73 0.35229231198161415 1102 74-75 0.3637464103115846 1102 76-77 0.48711283983421083 1102 78-79 0.42478780744085043 1102 80-81 0.35758234545512835 1102 82-83 0.3749169790625011 1102 84-85 0.36180950566942727 1102 86-87 0.34608860969681743 1102 88-89 0.44467468805438415 1102 90-91 0.39941585131053614 1102 92-93 0.47736205099412743 1102 94-95 0.3306283755423003 1102 96-97 0.2005612711830338 1102 98-99 0.4412248691329488 1102 100-101 0.4165709441935803 1102 102-103 0.31344491925571205 1102 104-105 0.20757882133953132 1102 106-107 0.17863605584951614 1102 108-109 0.19493118451342184 1102 110-111 0.3054283859888116 1102 112-113 0.30266280130037515 1102 114-115 0.3228876660540614 1102 116-117 0.24789793628785617 1102 118-119 0.13103652363244578 1102 120-121 0.07049501183470497 1102 122-123 0.02983576298612789 1102 124-125 -0.13122456058384202 1103 1 0.29693029168319285 1103 2 0.42119932119556935 1103 3 0.3400958710829052 1103 4 0.5156426480905623 1103 5 0.45400829279646615 1103 6 0.48958819319293667 1103 7 0.47489663048814634 1103 8 0.3854615327405426 1103 9 0.49576523655882454 1103 10-11 0.5442483696674643 1103 12-13 0.5860114495844115 1103 14-15 0.6249189428538955 1103 16-17 0.5749182930997208 1103 18-19 0.6034936571627654 1103 20-21 0.43269513749785204 1103 22-23 0.5470104810895009 1103 24-25 0.6026713561832935 1103 26-27 0.5420682753027677 1103 28-29 0.4739997763176049 1103 30-31 0.536882420393809 1103 32-33 0.5213872334408123 1103 34-35 0.5189558407213255 1103 36-37 0.5580705144067011 1103 38-39 0.44981632220236634 1103 40-41 0.5095138354023874 1103 42-43 0.39364059663497386 1103 44-45 0.42955211631895196 1103 46-47 0.5705459926743686 1103 48-49 0.489338925376714 1103 50-51 0.3071972887263641 1103 52-53 0.34329712251949474 1103 54-55 0.3696739484841771 1103 56-57 0.3187156145335095 1103 58-59 0.3083559857476388 1103 60-61 0.28641099905352974 1103 62-63 0.22912689596442704 1103 64-65 0.17349080889442092 1103 66-67 0.3210275212568092 1103 68-69 0.426569074349878 1103 70-71 0.3666350731899115 1103 72-73 0.31841975051238336 1103 74-75 0.383583696088607 1103 76-77 0.47795594845498357 1103 78-79 0.371577968131227 1103 80-81 0.3783859164112826 1103 82-83 0.37087921655833256 1103 84-85 0.4047920001005565 1103 86-87 0.33483776973639934 1103 88-89 0.40348223526412497 1103 90-91 0.3179694526904555 1103 92-93 0.48048346297421674 1103 94-95 0.416977629763263 1103 96-97 0.2611783047867391 1103 98-99 0.46824745414752655 1103 100-101 0.5949520825185317 1103 102-103 0.5797679296738671 1103 104-105 0.4933756858400251 1103 106-107 0.4511383971565195 1103 108-109 0.5189224383428446 1103 110-111 0.5630531763293973 1103 112-113 0.5510774586758878 1103 114-115 0.48750105938834665 1103 116-117 0.4429076454366765 1103 118-119 0.3898961219329493 1103 120-121 0.4637608440965906 1103 122-123 0.3288362100831712 1103 124-125 0.16639954191513695 1104 1 0.057395366757358346 1104 2 0.09712370030237238 1104 3 0.23100167148586692 1104 4 0.23121003259379336 1104 5 0.250036145665284 1104 6 0.15597896357635221 1104 7 0.13578719331656686 1104 8 0.11298231355232957 1104 9 0.21707835099756778 1104 10-11 0.32723252967718963 1104 12-13 0.23117465868848086 1104 14-15 0.333709150307655 1104 16-17 0.2766313812144148 1104 18-19 0.30646283271379104 1104 20-21 0.2624139764048081 1104 22-23 0.26017453053821527 1104 24-25 0.11679775211723609 1104 26-27 0.3130395567777242 1104 28-29 0.441033334417817 1104 30-31 0.3574201144071125 1104 32-33 0.41079079123728235 1104 34-35 0.5102537175855204 1104 36-37 0.4628806015798048 1104 38-39 0.3026686847857647 1104 40-41 0.3374304894132578 1104 42-43 0.3557004281610645 1104 44-45 0.24864578437269813 1104 46-47 0.40480889398955355 1104 48-49 0.4020304075219201 1104 50-51 0.4614498882262055 1104 52-53 0.4762114225529146 1104 54-55 0.4522352784561292 1104 56-57 0.4170808657763061 1104 58-59 0.35670237985915065 1104 60-61 0.32958243948525023 1104 62-63 0.2551472059807338 1104 64-65 0.3177826666635539 1104 66-67 0.3188064296187463 1104 68-69 0.26748322757609344 1104 70-71 0.2498347613511811 1104 72-73 0.19844852978523164 1104 74-75 0.30458054226504316 1104 76-77 0.36564464632577653 1104 78-79 0.38893918470727584 1104 80-81 0.4090477384404565 1104 82-83 0.4388180435182072 1104 84-85 0.3969624938789664 1104 86-87 0.35765489859261734 1104 88-89 0.45520266373864615 1104 90-91 0.35252989169459426 1104 92-93 0.33457166372546254 1104 94-95 0.3391381760885288 1104 96-97 0.23276921387764915 1104 98-99 0.3694569682663307 1104 100-101 0.3281958128387714 1104 102-103 0.3587851314274495 1104 104-105 0.35067655959596067 1104 106-107 0.24613733406992466 1104 108-109 0.25086135642442997 1104 110-111 0.3215458460942031 1104 112-113 0.39144741281129214 1104 114-115 0.3254974195823337 1104 116-117 0.20575327513830288 1104 118-119 0.23140573021084876 1104 120-121 0.11234934352454573 1104 122-123 -0.016701737045988096 1104 124-125 0.15775120589878 1105 1 0.08468995468403051 1105 2 0.1514534310319675 1105 3 0.09193187834530647 1105 4 0.24931894189129622 1105 5 0.249358105052508 1105 6 0.17268554795727908 1105 7 0.18982264601226717 1105 8 0.22315700660232807 1105 9 0.2543429585988264 1105 10-11 0.2833445643275212 1105 12-13 0.3762564319478585 1105 14-15 0.3262149698059176 1105 16-17 0.41232868802988776 1105 18-19 0.3858890746271513 1105 20-21 0.3124913895447392 1105 22-23 0.2935988097972597 1105 24-25 0.29914082985708745 1105 26-27 0.34680435776169816 1105 28-29 0.3086493580369378 1105 30-31 0.3331310661142908 1105 32-33 0.332293326832918 1105 34-35 0.35059106831449327 1105 36-37 0.4463953262917144 1105 38-39 0.3550823051743137 1105 40-41 0.3689062276907862 1105 42-43 0.2878636359597877 1105 44-45 0.3126218289395979 1105 46-47 0.2853968735126102 1105 48-49 0.26565956434929205 1105 50-51 0.19974603042134476 1105 52-53 0.3429437112192417 1105 54-55 0.32885200594026287 1105 56-57 0.2761952570531818 1105 58-59 0.2316765554602611 1105 60-61 0.21072594386819077 1105 62-63 0.18112727194705514 1105 64-65 0.11619891033057428 1105 66-67 0.21580371265174847 1105 68-69 0.2883373305325634 1105 70-71 0.2710903436253105 1105 72-73 0.27348642558918357 1105 74-75 0.31450461853229683 1105 76-77 0.3137412079572073 1105 78-79 0.35578546106918196 1105 80-81 0.30059307047016404 1105 82-83 0.3341448482833478 1105 84-85 0.3551347750502174 1105 86-87 0.3181826051697918 1105 88-89 0.3753292993840063 1105 90-91 0.4677904899077845 1105 92-93 0.4891598186142012 1105 94-95 0.28331333277656867 1105 96-97 0.30363496784199384 1105 98-99 0.3171151156840075 1105 100-101 0.400962362675056 1105 102-103 0.33508734175577004 1105 104-105 0.3207118489373322 1105 106-107 0.4029650048532787 1105 108-109 0.3473797469998452 1105 110-111 0.3354733047571443 1105 112-113 0.37812112213185856 1105 114-115 0.4529468337888751 1105 116-117 0.32219248690557833 1105 118-119 0.3518182960030103 1105 120-121 0.5578316351937183 1105 122-123 0.6998052187258246 1105 124-125 0.5668021772565162 1106 1 0.016649910792075673 1106 2 0.022143873663125646 1106 3 0.04056869555428122 1106 4 -0.5859110572646244 1106 5 -0.04972520525181068 1106 6 0.040575508193199994 1106 7 -0.10011689270596236 1106 8 -0.03502330422578126 1106 9 0.13097951217857684 1106 10-11 0.1931012738647695 1106 12-13 0.10632669838681608 1106 14-15 0.10342254660238837 1106 16-17 0.21330625526401548 1106 18-19 0.03691537858009042 1106 20-21 0.15119279591416301 1106 22-23 0.10907280473974623 1106 24-25 0.0687768712098773 1106 26-27 -0.02266853410060321 1106 28-29 -0.05610364565965398 1106 30-31 0.021741011642475883 1106 32-33 0.12663362360079589 1106 34-35 0.03636786686129767 1106 36-37 -0.007442964928394247 1106 38-39 -0.033461212726457745 1106 40-41 -0.20320966279208363 1106 42-43 -0.2530035191917861 1106 44-45 -0.21524626625829768 1106 46-47 -0.26998174083830406 1106 48-49 -0.30451581846231335 1106 50-51 -0.33337875061030076 1106 52-53 -0.3284063247985216 1106 54-55 -0.3127642955960681 1106 56-57 -0.3065716010530153 1106 58-59 -0.25802340441722293 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-0.9886864021010098 2216 54-55 -1.0524135499731813 2216 56-57 -1.0236005029538475 2216 58-59 -1.3430169625431176 2216 60-61 -1.1530342681314636 2216 62-63 -1.2276452871776158 2216 64-65 -0.9694269376192182 2216 66-67 -1.0301263723628367 2216 68-69 -1.2774479563794827 2216 70-71 -1.1209189191585978 2216 72-73 -1.2391715736885303 2216 74-75 -1.1207835135868223 2216 76-77 -1.140506349459372 2216 78-79 -1.500549638927886 2216 80-81 -1.4252963617572973 2216 82-83 -1.5453123174414216 2216 84-85 -1.449541271527238 2216 86-87 -1.3557212824664902 2216 88-89 -1.3169083608114036 2216 90-91 -1.33490294025043 2216 92-93 -1.3589417499099028 2216 94-95 -1.377527491796652 2216 96-97 -1.4407361096276752 2216 98-99 -1.3301590002520776 2216 100-101 -1.4780455726709079 2216 102-103 -1.413870229032785 2216 104-105 -1.6671481942044046 2216 106-107 -1.457824332696621 2216 108-109 -1.6350143751830117 2216 110-111 -1.8378986316429575 2216 112-113 -2.0251628227013825 2216 114-115 -1.9292967315532827 2216 116-117 -1.9027853077905235 2216 118-119 -2.114946220409397 2216 120-121 -1.9716072294564135 2216 122-123 -1.8214584338758577 2216 124-125 -1.7081114416711891 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16647.0 3 3717.0 4 2408.0 5 1899.0 6 2142.0 7 2338.0 8 2652.0 9 3355.0 10 4321.0 11 5138.0 12 4752.0 13 4286.0 14 4220.0 15 4246.0 16 3996.0 17 3847.0 18 3651.0 19 3482.0 20 3541.0 21 3825.0 22 4624.0 23 6214.0 24 9599.0 25 15016.0 26 17642.0 27 14909.0 28 16695.0 29 21306.0 30 23894.0 31 30137.0 32 41074.0 33 56038.0 34 91518.0 35 128288.0 36 259979.0 37 388647.0 38 56204.0 39 122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 69.3036392800534 10.291730864752166 6.740324412567745 13.664305442626684 2 11.339529564520994 32.0276650560046 42.78748264418164 13.84532273529277 3 37.705517451066335 19.778638583855543 18.956292373968907 23.559551591109216 4 14.691733515721934 15.378455030600374 47.31886387276184 22.610947580915855 5 16.82107637199841 33.33346494747016 27.8269926906773 22.01846598985413 6 31.229128318839138 38.6013081495204 14.594877164554724 15.574686367085738 7 44.46984521062631 23.251413888244 16.32419063683011 15.954550264299577 8 41.65421632326882 24.503776165462696 15.473592110957037 18.368415400311445 9 55.21423850394316 10.886637307135597 10.548031418962402 23.351092769958832 10-11 39.66146518115968 25.351576041422362 19.13908979136413 15.847868986053829 12-13 29.238594753977708 29.008409081397286 23.7987111181654 17.954285046459603 14-15 25.0814336105827 29.268325424895902 22.025847126706356 23.624393837815045 16-17 23.481465512816566 33.78790068297629 21.040707724209927 21.68992607999722 18-19 23.75212122217142 26.50822943391697 24.94154547371264 24.798103870198972 20-21 23.959525225270042 27.949633953452746 22.263929391828132 25.82691142944908 22-23 24.94699412256617 24.371174594450746 22.2865136464964 28.395317636486677 24-25 22.11381516761702 23.582857761047528 32.6418287244871 21.66149834684835 26-27 18.717611286877975 23.136512614348813 31.281895170826836 26.86398092794638 28-29 22.209222342173867 24.376683611700997 23.403416858111072 30.010677188014068 30-31 22.52250331459472 27.956938301553496 21.9367735628399 27.583784821011886 32-33 29.78041945120261 24.940203052980607 19.930367846970356 25.349009648846426 34-35 28.626766763873725 19.23258544705374 25.890400033481555 26.250247755590983 36-37 24.870949936392947 25.61895466487256 28.348096013089393 21.1619993856451 38-39 22.834497685903557 29.472531308015277 24.583632416775835 23.109338589305327 40-41 20.91688915316152 29.032849035312775 26.385082073234578 23.66517973829113 42-43 21.67602807712444 22.62519850059501 23.97764790515245 31.7211255171281 44-45 23.273429782314633 23.981122405870643 23.18064481995374 29.564802991860983 46-47 24.84785240320949 22.340763237255494 23.781575512350667 29.029808847184352 48-49 18.28981126354167 22.89214281145543 26.00272906238229 32.815316862620605 50-51 22.910462906151366 24.8122782538107 25.02825005981669 27.249008780221246 52-53 24.67748341912981 22.14883655553792 28.277539958732405 24.896140066599862 54-55 21.147193274630062 24.309739104479025 28.266405763249104 26.27666185764181 56-57 20.984219775759488 26.252572819731455 26.031862099626213 26.731345304882844 58-59 23.33032473157508 23.21673224786772 30.352211717121946 23.100731303435253 60-61 25.31774913858012 28.423519694306986 27.33209172527586 18.926639441837033 62-63 26.388714505803602 25.63664303216519 25.533671465425954 22.440970996605255 64-65 30.51656743054788 20.323175450510924 27.529454964701422 21.630802154239777 66-67 30.42293771520691 24.415128771759562 23.38178026380157 21.780153249231958 68-69 23.682043988266322 28.71031798523142 24.900978820069504 22.706659206432754 70-71 24.726207325780507 28.204792023404078 22.780553572218395 24.28844707859702 72-73 23.01025452317178 28.466014618183657 23.32220965785617 25.201521200788395 74-75 21.04887409096276 29.069922175316197 25.716222827599903 24.164980906121137 76-77 22.5538017140425 28.74349613815312 23.058396368181953 25.644305779622428 78-79 23.522476597119045 30.35944629863533 19.717368927480976 26.40070817676465 80-81 22.58889972926265 24.014209268874918 24.403633573958043 28.993257427904386 82-83 24.967683195024513 19.89941320420825 27.56455661817369 27.56834698259354 84-85 26.490474477301728 21.07486107913405 30.84858721497577 21.586077228588454 86-87 28.46822281343059 23.450743208934774 24.4647734837519 23.61626049388273 88-89 23.77653031203537 26.1349119062334 26.774462976528735 23.314094805202497 90-91 20.416498205892758 28.4591050942538 26.315020594329606 24.809376105523828 92-93 24.06922717877962 23.31704285097131 27.02094253656079 25.592787433688287 94-95 22.913315057933964 22.988383195793975 28.168479595761436 25.929822150510628 96-97 22.580980152533105 22.016532645756378 28.066643896602848 27.335843305107666 98-99 21.67010838449845 24.588829881744417 29.165577557104218 24.57548417665291 100-101 20.70101002204352 28.941212144902096 25.880270081497457 24.477507751556928 102-103 22.032164400739436 27.38992347412168 25.444795316373032 25.13311680876585 104-105 22.315959791860216 24.17214529354003 25.19274724693324 28.31914766766651 106-107 21.51039244191785 26.093632660087668 22.904435019303467 29.491539878691015 108-109 23.714217577972928 26.284045171667973 24.892981429583322 25.108755820775773 110-111 25.019247944319545 27.45668916405882 22.729670419917102 24.794392471704533 112-113 21.86115488289802 28.624748144968997 24.33893853493515 25.17515843719783 114-115 22.815652437608687 25.28145389415628 26.433330016536598 25.469563651698437 116-117 26.07774669152514 24.761147817105442 24.342825827227294 24.818279664142125 118-119 23.797809457579973 26.074132317612847 24.963372423820964 25.16468580098622 120-121 21.56845093556653 26.044482348807 26.51978726176516 25.867279453861308 122-123 22.105091501082093 27.089706083843712 28.130181201638006 22.67502121343619 124-125 22.67428881739769 28.978355793953366 23.44899813813281 24.898357250516128 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 7.5 2 13.5 3 23.5 4 23.5 5 26.0 6 35.0 7 37.0 8 52.0 9 63.0 10 68.0 11 92.0 12 129.0 13 175.0 14 212.5 15 246.0 16 310.5 17 400.5 18 515.0 19 607.0 20 716.5 21 879.5 22 1057.0 23 1310.5 24 1605.5 25 1898.5 26 2316.5 27 2784.0 28 3097.0 29 3491.5 30 4111.5 31 4841.0 32 5675.0 33 6671.0 34 8062.0 35 9022.5 36 9783.0 37 11134.0 38 11917.0 39 12346.5 40 12694.5 41 13502.5 42 14771.5 43 16134.0 44 17914.0 45 22841.5 46 41680.5 47 77948.0 48 109938.0 49 131951.0 50 132017.5 51 103995.0 52 75328.0 53 57168.0 54 47495.0 55 42140.5 56 37635.5 57 32861.5 58 28962.0 59 24733.5 60 21885.0 61 19055.0 62 14729.5 63 11062.0 64 8598.5 65 7428.5 66 6572.0 67 5722.0 68 4843.5 69 3936.5 70 3124.5 71 2416.5 72 2011.0 73 1679.5 74 1294.0 75 1035.5 76 773.5 77 576.0 78 488.0 79 377.5 80 365.5 81 349.0 82 230.0 83 105.5 84 49.5 85 48.5 86 35.5 87 23.5 88 15.0 89 13.5 90 15.0 91 13.0 92 9.5 93 8.5 94 4.0 95 1.0 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16211704487396641 2 0.017609401367216036 3 0.009002115497141827 4 0.01642491248601316 5 0.003237602941954517 6 0.0 7 2.3689777624057441E-4 8 3.948296270676241E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0010660399930825849 28-29 0.009317979198795928 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.3431258977438646E-4 58-59 0.0 60-61 3.9482962706762405E-5 62-63 0.0 64-65 0.0022900118369922197 66-67 3.9482962706762405E-5 68-69 0.007857109578645717 70-71 0.020925970234584073 72-73 7.896592541352481E-5 74-75 0.009910223639397364 76-77 4.7379555248114883E-4 78-79 0.001460869620150209 80-81 9.870740676690601E-4 82-83 0.0 84-85 0.02136028282435846 86-87 0.0028427733148868933 88-89 0.019465100614433864 90-91 7.896592541352481E-5 92-93 0.015003525828569713 94-95 0.014687662126915616 96-97 0.006830552548269896 98-99 0.0034745007181950917 100-101 8.291422168420104E-4 102-103 0.0 104-105 6.712103660149608E-4 106-107 0.001263454806616397 108-109 0.0 110-111 0.0 112-113 0.0012239718439096346 114-115 0.006870035510976659 116-117 0.0 118-119 0.07335934470916454 120-121 0.0018951822099245953 122-123 0.0057250295924805485 124-125 0.24645265321561094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1266369.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.87819667740177 #Duplication Level Percentage of deduplicated Percentage of total 1 81.43352908997764 33.28855818275024 2 9.686450725345393 7.919292757132601 3 2.9143362164961584 3.573984271260147 4 1.4528304459430506 2.3755635483270936 5 0.8792308676919025 1.7970686167176102 6 0.5545699968325063 1.3601892841143162 7 0.421802847036906 1.2069777818183862 8 0.31906590324136014 1.043427099660253 9 0.23810762017969891 0.876006911528443 >10 1.750771945859029 14.199647721633465 >50 0.1934002668191218 5.496802218591661 >100 0.1342668302919943 11.00962939850551 >500 0.012943708634961538 3.563474213679417 >1k 0.007341207882515499 6.14208290603277 >5k 5.795690433564867E-4 1.9269279705200846 >10k+ 7.727587244753157E-4 4.220367117728027 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 14500 1.1450059184961097 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 14416 1.1383727807613737 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 12967 1.0239511548371762 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 11558 0.9126881659295198 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 9793 0.7733133075746484 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 8263 0.6524954416919555 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 6344 0.5009598308234015 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 4866 0.3842481930622117 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 4556 0.35976875618401905 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3731 0.29462186771786103 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3653 0.28846252553560614 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 3600 0.28427733148868933 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 2824 0.22299977336779406 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2738 0.21620870378223092 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 2647 0.20902280456960015 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 2593 0.2047586445972698 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2592 0.20467967867185632 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2525 0.19938896166915013 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 2325 0.1835957765864452 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 2317 0.182964049183137 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 2076 0.1639332611584775 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1885 0.14885076940449427 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1849 0.14600799608960738 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1777 0.14032244945983358 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1751 0.13826933539908196 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1723 0.13605828948750326 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1650 0.13029377693231592 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1623 0.12816169694615076 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1592 0.12571375325833148 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1573 0.12421340067547453 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1565 0.12358167327216633 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1518 0.11987027477773067 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1455 0.1148954214766786 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1395 0.11015746595186711 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1349 0.10652503338284497 No Hit ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA 1342 0.1059722719049503 No Hit CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 1339 0.10573537412870973 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA 1287 0.10162914600720643 No Hit ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA 1284 0.10139224823096586 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 1282 0.1012343163801388 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.763807389473368E-4 0.0 22-23 0.0 0.0 0.0 0.0018951822099245955 0.0 24-25 0.0 0.0 0.0 0.003237602941954517 0.0 26-27 0.0 0.0 0.0 0.004935370338345301 0.0 28-29 0.0 0.0 0.0 0.0105024680799988 0.0 30-31 0.0 0.0 0.0 0.03739036568330399 0.0 32-33 0.0 0.0 0.0 0.08986322312059122 0.0 34-35 0.0 0.0 0.0 0.148061110150359 0.0 36-37 0.0 0.0 0.0 0.2184592326565164 0.0 38-39 7.896592541352481E-5 0.0 0.0 0.3423962525930436 0.0 40-41 7.896592541352481E-5 0.0 0.0 0.5361786335578335 0.0 42-43 7.896592541352481E-5 0.0 0.0 0.9507102590161319 0.0 44-45 7.896592541352481E-5 0.0 0.0 1.5434679781327558 0.0 46-47 1.5793185082704962E-4 0.0 0.0 2.1492550749426114 0.0 48-49 1.5793185082704962E-4 0.0 0.0 2.3824019697260432 0.0 50-51 1.5793185082704962E-4 0.0 0.0 2.6855126744258584 0.0 52-53 1.5793185082704962E-4 0.0 0.0 2.9325575720820707 0.0 54-55 1.5793185082704962E-4 0.0 0.0 3.168507757217683 0.0 56-57 1.5793185082704962E-4 0.0 0.0 3.5415427888711744 0.0 58-59 1.5793185082704962E-4 0.0 0.0 3.965155495752028 0.0 60-61 1.5793185082704962E-4 0.0 0.0 4.568376199985944 0.0 62-63 1.5793185082704962E-4 0.0 0.0 5.304654488541649 0.0 64-65 1.5793185082704962E-4 0.0 0.0 5.955846992464282 0.0 66-67 1.5793185082704962E-4 0.0 0.0 6.498895661533092 0.0 68-69 2.3689777624057444E-4 0.0 0.0 6.963017888151084 0.0 70-71 2.3689777624057444E-4 0.0 0.0 7.496511680244858 0.0 72-73 2.3689777624057444E-4 0.0 0.0 8.002880676959085 0.0 74-75 2.3689777624057444E-4 0.0 0.0 8.508775878120833 0.0 76-77 2.3689777624057444E-4 0.0 0.0 9.101888943901816 0.0 78-79 2.3689777624057444E-4 0.0 0.0 9.864739266359173 0.0 80-81 2.3689777624057444E-4 0.0 0.0 11.771766365095797 0.0 82-83 2.3689777624057444E-4 0.0 0.0 13.128282514812033 0.0 84-85 2.763807389473368E-4 0.0 0.0 13.746190881172865 0.0 86-87 3.1586370165409924E-4 0.0 0.0 14.261877857085889 0.0 88-89 3.1586370165409924E-4 0.0 0.0 14.856688690263265 0.0 90-91 3.9482962706762403E-4 0.0 0.0 15.6059963565122 0.0 92-93 3.9482962706762403E-4 0.0 0.0 16.558009553297655 0.0 94-95 3.9482962706762403E-4 0.0 0.0 17.752606072953462 0.0 96-97 3.9482962706762403E-4 0.0 0.0 19.06162421853346 0.0 98-99 3.9482962706762403E-4 0.0 0.0 20.398161989120076 0.0 100-101 4.3431258977438646E-4 0.0 0.0 21.595324901351816 0.0 102-103 4.737955524811489E-4 0.0 0.0 22.65686383668583 0.0 104-105 4.737955524811489E-4 0.0 0.0 23.76021522952631 0.0 106-107 4.737955524811489E-4 0.0 0.0 24.80639529236739 0.0 108-109 4.737955524811489E-4 0.0 0.0 25.687220707392555 0.0 110-111 4.737955524811489E-4 0.0 0.0 26.681006878721764 0.0 112-113 4.737955524811489E-4 0.0 0.0 29.63764905805496 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGT 2410 0.0 93.67877 1 AGTAGTA 1980 0.0 93.1391 2 GAGTTAG 1245 0.0 86.602066 1 GAGTCCG 795 0.0 86.16874 1 GAGTCGG 680 0.0 84.97317 1 GAGTCGT 890 0.0 84.33339 1 AGTCGTA 530 0.0 84.18233 2 GAGTCAG 6005 0.0 83.7237 1 GAGTAAG 1180 0.0 81.27611 1 AGTCCGT 355 0.0 78.75987 2 GAGTACG 630 0.0 77.53402 1 GAGTAGC 795 0.0 75.678635 1 GAGTCGA 315 0.0 75.642944 1 GAGTTAC 575 0.0 75.6265 1 GAGTAGG 1200 0.0 73.468216 1 GAGTAAC 990 0.0 72.204636 1 GTGGGGT 7475 0.0 70.66199 7 AGTACGG 355 0.0 70.381165 2 TGGGGTA 7855 0.0 69.89395 8 AGTCAGT 5120 0.0 69.597305 2 >>END_MODULE