FastQCFastQC Report
Thu 26 May 2016
SRR935919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096589
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT117230.5591463085993487No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC113830.5429294916647945No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT99670.47539121878441604No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT88420.4217326333392No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG80520.38405238222655946No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT66120.3153693928566829No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA56820.27101162888863767No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC35510.16937034392529962No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC33450.1595448607237756No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC32020.15272425830718372No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA29180.1391784465147914No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG25530.12176921657034354No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG25080.1196228731525349No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT24280.1158071515208751No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG24220.11552097239850062No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT22340.10655402656410007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT24600.075.800931
AGTAGTA19050.074.012832
GAGTCAG59400.063.386371
GAGTTAG14250.062.71071
GAGTCGG11650.059.319481
AGTCGTA5700.058.447622
GGTATCA40000.058.2347221
TGGGGTA67700.056.146738
GGGGTAA78450.055.7322549
GAGTAAG16950.055.5331881
ACGGGAT7100.055.297845
TCGTAGG12000.055.0294842
GTGGGGT67650.054.5175257
AGGGTAA65550.053.8138776
CGGGATT15300.053.2649356
GAGTCGT8950.053.251541
GAGTCCG10750.053.2020071
GATTGTG50300.052.981269
GAGTAGG20800.051.555431
GTAGTAG37200.050.4467121
Produced by FastQC (version 0.11.5)