FastQCFastQC Report
Thu 26 May 2016
SRR935919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096589
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT124040.5916276389888528No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT108130.5157424750392184No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC96580.46065299398213005No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT91600.43690012682504775No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG87640.4180123047483317No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT71380.3404577625848461No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA48370.230708069154231No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC39650.1891167033691391No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC30630.1460944419721748No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA30020.1431849542280342No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC28550.1361735657298593No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG28510.13598277964827632No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG27450.13092694848632708No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG26380.1258234208039821No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT25950.12377247042696495No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT24040.11466243503137716No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA23000.10970199691021942No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA21000.10016269283106989No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT23400.073.9858861
AGTAGTA17250.070.4555052
GGTATCA45850.066.1441041
GAGTAAG15250.064.200841
AGGGTAA65450.062.3587766
GAGTCAG55050.059.6447331
GATTGTG54850.058.6818439
GAGTTAG14550.058.6732031
TAGTAGG37100.058.13112
GATAGCG19300.057.0292329
GTAGTAG38500.056.7527731
GAGTCGT8500.056.1872521
ACGGGAT7650.056.0135
GAGTCCG10150.055.28771
AGTCCGT3800.054.8729482
TCGTAGG11700.054.4843522
GAGTACG8900.054.332761
GAGTCGA5600.053.3026431
TGGGGTA64700.052.8745468
GGGGTAA76500.051.8737379
Produced by FastQC (version 0.11.5)