FastQCFastQC Report
Thu 26 May 2016
SRR935918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312044
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT10410.33360679904116086No Hit
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA9680.31021266231685274No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT9420.3018805040314827No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC7010.22464780607862994No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA6970.2233659355731884No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC6280.20125366935432182No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA4630.14837651100485827No Hit
GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT3740.11985489225878401No Hit
GGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAAT3360.10767712245708938No Hit
GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT3340.10703618720436861No Hit
ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA3170.10158823755624206No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAG250.00204902171.45631
GTATTAG300.00422046559.546921
GTCCTAG401.7630846E-459.546921
TAACAAC1850.057.9375461
GTATGAG1103.6379788E-1254.1335641
TCCTAGG453.1675538E-452.888182
GTGTACG803.8109647E-852.1035541
TGTTAGG350.00779137650.9993132
GAGACGC350.007796315550.991146
TGTACGG856.182745E-848.999342
GTATAGG1251.0913936E-1147.637531
AGTCTTG505.33116E-447.591737
GTAACAA909.633732E-846.3142741
TATAAGG802.2887052E-644.62442
GTCCAAG558.485835E-443.306851
GGAAACA3500.040.8321761
TATGAGG1401.884473E-938.2494852
ATGAGGG1401.884473E-938.2494853
GGAGGTA1401.886292E-938.2433559
TGTATGG801.1955641E-437.1872
Produced by FastQC (version 0.11.5)