##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935918_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 312044 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.96555934419505 31.0 30.0 34.0 10.0 34.0 2 27.897982335824434 31.0 29.0 34.0 7.0 34.0 3 27.907420107420748 31.0 28.0 34.0 7.0 34.0 4 30.845137224237607 37.0 32.0 37.0 7.0 37.0 5 31.133263257745703 37.0 33.0 37.0 7.0 37.0 6 31.32468818499955 37.0 33.0 37.0 8.0 37.0 7 31.28355936983246 37.0 33.0 37.0 7.0 37.0 8 30.986245529476612 37.0 32.0 37.0 7.0 37.0 9 32.0917659048083 39.0 33.0 39.0 7.0 39.0 10-11 32.17577008370614 39.0 32.0 39.0 7.0 39.0 12-13 32.172276986578815 39.0 32.0 39.0 7.0 39.0 14-15 33.15333254284652 39.5 32.0 41.0 4.5 41.0 16-17 33.30334183640768 39.0 32.5 41.0 2.0 41.0 18-19 33.52653311712451 39.5 32.5 41.0 2.0 41.0 20-21 33.74431490430837 40.0 33.0 41.0 2.0 41.0 22-23 33.5109888349079 40.0 32.0 41.0 2.0 41.0 24-25 33.217821845637154 39.0 32.0 41.0 2.0 41.0 26-27 33.01842528617759 39.0 32.0 41.0 2.0 41.0 28-29 32.769463601287 39.0 32.0 41.0 2.0 41.0 30-31 32.47656900949866 39.0 31.0 41.0 2.0 41.0 32-33 32.111043634872004 39.0 31.0 41.0 2.0 41.0 34-35 31.73298637371653 39.0 31.0 41.0 2.0 41.0 36-37 31.496518119239596 39.0 31.0 41.0 2.0 41.0 38-39 31.319634410531847 39.0 30.5 41.0 2.0 41.0 40-41 31.056532091628103 38.0 30.0 41.0 2.0 41.0 42-43 30.758434707925804 38.0 30.0 41.0 2.0 41.0 44-45 30.40366743151607 37.0 30.0 40.5 2.0 41.0 46-47 30.024429247157453 36.5 29.5 40.0 2.0 41.0 48-49 29.634343233646536 36.0 29.0 39.5 2.0 41.0 50-51 29.22149921164964 35.0 28.0 39.0 2.0 41.0 52-53 28.87867576367435 35.0 28.0 39.0 2.0 41.0 54-55 28.509990578251784 35.0 27.0 37.5 2.0 40.5 56-57 28.18108503928933 35.0 27.0 37.0 2.0 40.0 58-59 27.8480743100332 35.0 26.0 36.0 2.0 39.0 60-61 27.54787946571637 35.0 26.0 36.0 2.0 39.0 62-63 27.260083513863428 35.0 26.0 35.0 2.0 37.5 64-65 27.045250028842087 35.0 25.0 35.0 2.0 37.0 66-67 26.877459589032316 35.0 25.0 35.0 2.0 36.0 68-69 26.731021907166937 35.0 24.5 35.0 2.0 36.0 70-71 26.56808815423466 35.0 24.0 35.0 2.0 35.0 72-73 26.40722782684493 35.0 24.0 35.0 2.0 35.0 74-75 26.23335170681058 35.0 22.0 35.0 2.0 35.0 76-77 26.0329568906949 34.0 20.0 35.0 2.0 35.0 78-79 25.77579764392201 34.0 18.0 35.0 2.0 35.0 80-81 25.459428798502778 34.0 9.0 35.0 2.0 35.0 82-83 25.15099953852662 34.0 2.0 35.0 2.0 35.0 84-85 24.88714732537719 34.0 2.0 35.0 2.0 35.0 86-87 24.621285780210485 34.0 2.0 35.0 2.0 35.0 88-89 24.256635282203792 33.0 2.0 35.0 2.0 35.0 90-91 23.856478573534503 33.0 2.0 35.0 2.0 35.0 92-93 23.43385067490482 33.0 2.0 35.0 2.0 35.0 94-95 23.05248138083091 32.0 2.0 35.0 2.0 35.0 96-97 22.677285895578827 32.0 2.0 35.0 2.0 35.0 98-99 22.24087628667752 31.0 2.0 35.0 2.0 35.0 100-101 21.879799002704747 30.5 2.0 35.0 2.0 35.0 102-103 21.198605004422454 29.0 2.0 34.5 2.0 35.0 104-105 21.45721757188089 30.0 2.0 35.0 2.0 35.0 106-107 21.372639755931857 30.0 2.0 35.0 2.0 35.0 108-109 21.09066349617362 29.5 2.0 35.0 2.0 35.0 110-111 20.68250791555037 29.0 2.0 35.0 2.0 35.0 112-113 20.200093576546898 29.0 2.0 35.0 2.0 35.0 114-115 19.59963979438797 27.0 2.0 35.0 2.0 35.0 116-117 18.91116477163477 25.0 2.0 34.0 2.0 35.0 118-119 18.06206977221161 23.5 2.0 34.0 2.0 35.0 120-121 17.165866031713477 10.5 2.0 34.0 2.0 35.0 122-123 16.220981656433068 2.0 2.0 34.0 2.0 35.0 124-125 14.001990103959699 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2840323242328999 1101 2 0.3419906601545861 1101 3 0.2617839434101761 1101 4 -0.07450621675368652 1101 5 0.28071500175258635 1101 6 -0.06739578355153597 1101 7 -0.047759049226286265 1101 8 -0.1343468067253859 1101 9 -0.3933033183015162 1101 10-11 -0.14292880085641713 1101 12-13 0.06343400607956795 1101 14-15 -0.12946123302928925 1101 16-17 0.25868457855652593 1101 18-19 0.08534875604068048 1101 20-21 0.1401456150449789 1101 22-23 0.17892324416929029 1101 24-25 0.5419179377504619 1101 26-27 0.6228891133623051 1101 28-29 0.47493697224545883 1101 30-31 0.31735598662327646 1101 32-33 0.3817241817743735 1101 34-35 0.012709728199638448 1101 36-37 0.24370846301681226 1101 38-39 0.14993378026272453 1101 40-41 -0.05305495252575554 1101 42-43 0.14446078082091418 1101 44-45 -0.020957622627388872 1101 46-47 0.25244155980351124 1101 48-49 0.40998871338646126 1101 50-51 0.48437394229608444 1101 52-53 0.5223103133615261 1101 54-55 0.08367376374395619 1101 56-57 0.18926265666558706 1101 58-59 0.253176699271215 1101 60-61 0.3414075104780423 1101 62-63 0.5156874863767875 1101 64-65 0.5051032629501861 1101 66-67 0.3824300113442405 1101 68-69 0.4792805464557688 1101 70-71 0.2354346027772216 1101 72-73 0.5341468644292675 1101 74-75 0.7164272529389599 1101 76-77 0.6949092161039907 1101 78-79 0.5066521693803594 1101 80-81 0.3041416974582738 1101 82-83 0.22662378534715089 1101 84-85 0.10278214259540164 1101 86-87 0.13029607277016808 1101 88-89 0.032372608316158846 1101 90-91 0.032378461706585426 1101 92-93 -0.22217144102038233 1101 94-95 -0.006691577406385818 1101 96-97 -0.06802979674123932 1101 98-99 -0.30784822690704416 1101 100-101 -0.036959265748102155 1101 102-103 -0.051308807811178525 1101 104-105 0.16092486917062843 1101 106-107 0.24188694782393227 1101 108-109 0.12497355613334449 1101 110-111 -0.11866908801063758 1101 112-113 0.16213097574404856 1101 114-115 0.17654016877380485 1101 116-117 -0.39232416476080445 1101 118-119 -0.31654269188366513 1101 120-121 -0.5403886660427268 1101 122-123 -0.6199772002158888 1101 124-125 -0.4474807451682814 1102 1 0.6229512936130348 1102 2 0.8706734458155267 1102 3 0.8155320763451996 1102 4 1.0311229841425025 1102 5 0.9568259054120318 1102 6 0.8074175099510761 1102 7 1.1208135796012009 1102 8 0.8407745524979156 1102 9 0.9534106473444908 1102 10-11 1.140491662175691 1102 12-13 1.2057977103364763 1102 14-15 1.2035181359027547 1102 16-17 1.0539258967044063 1102 18-19 1.3467028449129756 1102 20-21 1.3014483596304913 1102 22-23 1.1750140768802666 1102 24-25 1.1931618697893 1102 26-27 1.4773163911815743 1102 28-29 1.2650088542469575 1102 30-31 1.3856672263544212 1102 32-33 1.6473375126182788 1102 34-35 1.5789438581473583 1102 36-37 1.23415142418186 1102 38-39 1.1009933396353908 1102 40-41 1.1621592931799967 1102 42-43 0.9482672595363724 1102 44-45 1.0809257791649927 1102 46-47 1.4072679227609441 1102 48-49 1.5325381906082711 1102 50-51 1.435666884790482 1102 52-53 1.2651571206804206 1102 54-55 1.309156866805946 1102 56-57 1.4891016223115905 1102 58-59 1.2838199031547113 1102 60-61 1.3680948704481715 1102 62-63 1.1985548314103944 1102 64-65 1.1457399321062702 1102 66-67 0.9715240180656721 1102 68-69 1.2696674956716052 1102 70-71 1.143960088214115 1102 72-73 1.1483995716734796 1102 74-75 1.2523565845297 1102 76-77 1.2268593654691848 1102 78-79 1.2783451305454108 1102 80-81 1.4016491657181689 1102 82-83 1.0468487666765007 1102 84-85 1.1205776056275134 1102 86-87 1.2246995455110188 1102 88-89 0.9894184447612702 1102 90-91 1.2696357432599825 1102 92-93 1.092499022011733 1102 94-95 0.8697553979483601 1102 96-97 0.8416275968360551 1102 98-99 1.0360418029659968 1102 100-101 0.8222873735349445 1102 102-103 0.7593474655271351 1102 104-105 0.7759244024043852 1102 106-107 0.7715966192204995 1102 108-109 1.165605557627 1102 110-111 1.3389952137070615 1102 112-113 0.9197859421368761 1102 114-115 0.9425035743003214 1102 116-117 0.8018972691450941 1102 118-119 0.5254219272350795 1102 120-121 0.22254075890126046 1102 122-123 0.30520017095662766 1102 124-125 0.4106339859743624 1103 1 0.6036981630226954 1103 2 0.46802362470640446 1103 3 0.5294967453970791 1103 4 0.7376748624967071 1103 5 0.555551307916506 1103 6 0.29807046165054274 1103 7 0.5942594651110369 1103 8 0.6399024594746585 1103 9 0.5498328298131838 1103 10-11 0.6455900521578002 1103 12-13 0.5122825046119743 1103 14-15 0.6079512754376424 1103 16-17 0.5358746891909902 1103 18-19 0.6382670220149436 1103 20-21 0.5394682612405077 1103 22-23 0.7526024042505739 1103 24-25 0.4457054744404658 1103 26-27 0.1112329833103729 1103 28-29 -0.09188962518059896 1103 30-31 -0.03583322087276741 1103 32-33 0.31771989186694327 1103 34-35 0.060942963692976804 1103 36-37 0.15791595906558342 1103 38-39 -0.05440692870092434 1103 40-41 -0.09809898052304433 1103 42-43 -0.2026886885852761 1103 44-45 -0.07392104552194922 1103 46-47 0.19217400505074522 1103 48-49 0.04118085608233457 1103 50-51 0.08104926403914092 1103 52-53 0.2243085070847144 1103 54-55 0.40019555195800294 1103 56-57 0.3585615954779584 1103 58-59 0.29123269385238615 1103 60-61 0.3593348078363654 1103 62-63 -0.12075979291876493 1103 64-65 -0.032055237840062034 1103 66-67 0.41681136153615483 1103 68-69 0.603360474204699 1103 70-71 0.4081315998420223 1103 72-73 0.42689353410639796 1103 74-75 0.5187754127944579 1103 76-77 0.26656195938689464 1103 78-79 0.3022550142663434 1103 80-81 0.3315463034641404 1103 82-83 0.2794404482507389 1103 84-85 0.36048704212125315 1103 86-87 0.07965258665592145 1103 88-89 0.1418837846539347 1103 90-91 -0.2172828613911797 1103 92-93 -0.04237351823871549 1103 94-95 0.06297990599844638 1103 96-97 0.10203680971619988 1103 98-99 0.4921799961681437 1103 100-101 0.4694586615492575 1103 102-103 0.29822268914072936 1103 104-105 0.45110888362084367 1103 106-107 0.547390447485256 1103 108-109 0.36756217658943413 1103 110-111 0.48016247667665013 1103 112-113 0.4623172480402786 1103 114-115 0.5466180644613203 1103 116-117 0.1142631009876709 1103 118-119 -0.12664090818531193 1103 120-121 -0.1091005648912251 1103 122-123 -0.3154840866462365 1103 124-125 -0.4942463807519335 1104 1 0.5965254131154012 1104 2 0.3711279315348186 1104 3 0.32026858414264225 1104 4 0.570953455493477 1104 5 0.43850410761984904 1104 6 0.4262846906064155 1104 7 0.747461774800076 1104 8 0.7663155547758187 1104 9 0.9468397694752042 1104 10-11 1.0867856870574144 1104 12-13 0.9330405699825235 1104 14-15 0.8719762794107311 1104 16-17 0.8880413470285689 1104 18-19 0.608429124043866 1104 20-21 0.7929089562662028 1104 22-23 0.7604108483889505 1104 24-25 0.5799429062357646 1104 26-27 0.7942599473406773 1104 28-29 0.642127962359794 1104 30-31 0.637841091782839 1104 32-33 0.4329023890857826 1104 34-35 0.4844874887764128 1104 36-37 0.4246055813572589 1104 38-39 0.4334195267739922 1104 40-41 0.7047960769543096 1104 42-43 0.5854370729234404 1104 44-45 0.33004144851841843 1104 46-47 0.6479482276496746 1104 48-49 0.7844054150694575 1104 50-51 0.8416759526019391 1104 52-53 0.5873190490140452 1104 54-55 0.5817524730789394 1104 56-57 0.6447246510482252 1104 58-59 0.7128489464348924 1104 60-61 0.5887038813120213 1104 62-63 0.5242102764778558 1104 64-65 0.5120116160032389 1104 66-67 0.5115324506922718 1104 68-69 0.7806052694581815 1104 70-71 0.8627456804692386 1104 72-73 0.7359044998318822 1104 74-75 0.6412895834783612 1104 76-77 0.6509821532736346 1104 78-79 0.7342463264451808 1104 80-81 0.7305106516126862 1104 82-83 1.0451129597726982 1104 84-85 0.9654692956573037 1104 86-87 0.8127219303016275 1104 88-89 0.847030259199677 1104 90-91 0.6023521441711424 1104 92-93 1.039718445526713 1104 94-95 1.0780193720152909 1104 96-97 0.9022275179855228 1104 98-99 0.894357248909575 1104 100-101 0.8625613791420967 1104 102-103 0.7589367856603033 1104 104-105 0.6129036603332452 1104 106-107 0.9424558710165378 1104 108-109 1.1192096841383012 1104 110-111 1.3351950560059933 1104 112-113 1.1612657038330383 1104 114-115 1.1223265986563753 1104 116-117 1.0928666926600776 1104 118-119 1.2825157374681275 1104 120-121 1.1160596830295262 1104 122-123 1.0830935475117371 1104 124-125 0.5430344371746436 1105 1 0.9259871622387905 1105 2 0.9648880093418875 1105 3 0.8161965102259252 1105 4 0.94295314836031 1105 5 1.1024287576160106 1105 6 1.2474564036416602 1105 7 0.9427831128769135 1105 8 1.0035554513743214 1105 9 1.1913536032649716 1105 10-11 1.1844847477418128 1105 12-13 1.3647648667755448 1105 14-15 1.3675279673634293 1105 16-17 1.3879389693059636 1105 18-19 1.2498818639276692 1105 20-21 1.2659119508145906 1105 22-23 1.4785496213919416 1105 24-25 1.4611745102387417 1105 26-27 1.4743372425212442 1105 28-29 1.4303340487154088 1105 30-31 1.3152642445048102 1105 32-33 1.3872521625750664 1105 34-35 1.526621040515554 1105 36-37 1.6930348511481554 1105 38-39 1.8413077303009011 1105 40-41 1.746361432332975 1105 42-43 1.4594356908241863 1105 44-45 1.5928936918702625 1105 46-47 1.4625293747920587 1105 48-49 1.581538838836444 1105 50-51 1.5747388381180514 1105 52-53 1.5151760273355634 1105 54-55 1.5777367069096577 1105 56-57 1.6185824995803877 1105 58-59 1.5855755211322382 1105 60-61 1.5256008125397855 1105 62-63 1.4243408080741808 1105 64-65 1.3984516866438668 1105 66-67 1.287532553069994 1105 68-69 1.5798041637787748 1105 70-71 1.41365812192198 1105 72-73 1.657439653782383 1105 74-75 1.5786395361286623 1105 76-77 1.644691042219403 1105 78-79 1.6604729395341735 1105 80-81 1.6418285088469489 1105 82-83 1.5971566750602513 1105 84-85 1.6001395114183516 1105 86-87 1.7628639793917458 1105 88-89 1.3848025199557412 1105 90-91 1.5498323724734675 1105 92-93 1.2892308716227951 1105 94-95 1.017670263981202 1105 96-97 1.4304402588930998 1105 98-99 1.1834002731475017 1105 100-101 1.201938410700027 1105 102-103 0.9976739564519406 1105 104-105 0.8602021681779384 1105 106-107 0.7021983910441811 1105 108-109 0.7925556440574226 1105 110-111 0.7197104254615994 1105 112-113 0.8916744468905513 1105 114-115 0.6583635570142334 1105 116-117 0.5333903547112158 1105 118-119 0.24946308972427644 1105 120-121 0.10904680903090735 1105 122-123 -0.33355307018425506 1105 124-125 -0.20298690858052026 1106 1 0.25382736198910294 1106 2 0.4425217364138163 1106 3 0.2297948968580208 1106 4 -0.44590693038741236 1106 5 0.36120624729237605 1106 6 0.38113545866902854 1106 7 0.22311058814820584 1106 8 0.32528309950646417 1106 9 0.2076841516179968 1106 10-11 0.20032072200474715 1106 12-13 0.44084044537920875 1106 14-15 0.6211196316292558 1106 16-17 0.5631406402941508 1106 18-19 0.29502015260528935 1106 20-21 0.47439173569886606 1106 22-23 0.4037801025212957 1106 24-25 0.2883808868833171 1106 26-27 0.3893089125490974 1106 28-29 0.1701904781785757 1106 30-31 0.42065654259373986 1106 32-33 0.30999776902854137 1106 34-35 0.1232814649849665 1106 36-37 -0.028001353595918488 1106 38-39 0.014187784079833676 1106 40-41 -0.08209818545248027 1106 42-43 -0.1603064584123466 1106 44-45 -0.18897272510850982 1106 46-47 0.040280743273765296 1106 48-49 0.15192921624929667 1106 50-51 0.019267312140911486 1106 52-53 0.18008767623597421 1106 54-55 0.14823272150679756 1106 56-57 0.16525012658509297 1106 58-59 0.22318956127437062 1106 60-61 0.3497989302772311 1106 62-63 0.26359222457278975 1106 64-65 0.2947783936447337 1106 66-67 0.34158277874943366 1106 68-69 0.6228993332784398 1106 70-71 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28-29 -1.9969551156911578 2216 30-31 -1.7655356177363544 2216 32-33 -1.7459261920678344 2216 34-35 -1.8283468875054538 2216 36-37 -1.9580779819484064 2216 38-39 -2.1510789415317184 2216 40-41 -2.4758500274801847 2216 42-43 -2.3487458925414373 2216 44-45 -2.1664631465644497 2216 46-47 -2.3721492596734812 2216 48-49 -2.2395016708712454 2216 50-51 -2.3757002681714994 2216 52-53 -2.556391840415955 2216 54-55 -2.245777004399482 2216 56-57 -2.228059628793627 2216 58-59 -2.6001599819027597 2216 60-61 -2.2715170143145755 2216 62-63 -2.031242729744921 2216 64-65 -2.152686976234431 2216 66-67 -2.2866591900097752 2216 68-69 -2.580534206361996 2216 70-71 -2.3909417317092547 2216 72-73 -2.28855242508455 2216 74-75 -2.262918430206522 2216 76-77 -2.3682905755859274 2216 78-79 -2.6663986434009495 2216 80-81 -2.470311488570072 2216 82-83 -2.4876984629138335 2216 84-85 -2.5666046445935287 2216 86-87 -2.7576814594992953 2216 88-89 -2.76915158913204 2216 90-91 -2.742097973215113 2216 92-93 -2.663588879550307 2216 94-95 -2.4783325772947897 2216 96-97 -2.558967378112367 2216 98-99 -2.476714238884874 2216 100-101 -2.513910312878256 2216 102-103 -2.385297759937057 2216 104-105 -2.8819972377370853 2216 106-107 -2.6659125909180226 2216 108-109 -3.210122043316119 2216 110-111 -3.2308728971093252 2216 112-113 -3.2606222512023493 2216 114-115 -3.1129783001470734 2216 116-117 -2.9705938891219255 2216 118-119 -2.7598851387831935 2216 120-121 -2.060192073400536 2216 122-123 -2.038503778350771 2216 124-125 -1.8165755262567007 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27832.0 3 2861.0 4 1396.0 5 2994.0 6 8257.0 7 6935.0 8 4174.0 9 4270.0 10 3007.0 11 1935.0 12 1503.0 13 1233.0 14 1282.0 15 1395.0 16 1158.0 17 1085.0 18 1167.0 19 1519.0 20 1909.0 21 2630.0 22 3267.0 23 3863.0 24 4343.0 25 4642.0 26 5011.0 27 5315.0 28 5908.0 29 7381.0 30 9492.0 31 12895.0 32 17153.0 33 21727.0 34 28416.0 35 38743.0 36 50394.0 37 13873.0 38 1066.0 39 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 56.50556546969483 19.000590568871885 11.955810042238513 12.538033919194772 2 20.401341008448608 24.530134229048343 35.10019102319201 19.968333739311035 3 30.858424805704672 25.13933178431215 24.249659482413268 19.752583927569905 4 16.51004775487965 24.2296721258934 28.251658600685875 31.008621518541073 5 16.73535576251306 35.37435023106456 24.424261458687194 23.466032547735182 6 30.30021407237441 37.54246196049275 8.892976631500686 23.264347335632156 7 27.878119752342617 42.79492635653946 11.148427785825076 18.178526105292843 8 34.879055517811594 29.367332812039326 10.481855122995475 25.271756547153608 9 35.80104087885042 19.07711732960736 14.499557754675624 30.6222840368666 10-11 30.039834125956595 28.998154106472164 14.034398995013525 26.92761277255772 12-13 32.505512043173404 30.80238684288113 16.197875940572484 20.494225173372985 14-15 36.00934483598467 24.372844855212726 20.115592672828193 19.502217635974414 16-17 30.10889489943726 24.753079693889322 21.401949725038776 23.73607568163464 18-19 28.52290061657971 25.721693094563587 21.255816487418443 24.49958980143826 20-21 38.44938534309264 23.2866198356642 17.563548730307264 20.70044609093589 22-23 34.09887708143724 26.594486674956098 16.495910833087642 22.810725410519026 24-25 26.995391675532936 29.001679250362127 18.47543936111574 25.527489712989194 26-27 23.65955114872725 29.185748026701617 21.160360337263374 25.99434048730776 28-29 24.877972956948412 28.068944077481966 22.84205229776201 24.211030667807616 30-31 25.80966145799951 28.98950148056043 19.718533283767673 25.482303777672378 32-33 23.000442245324376 29.681070618246146 20.72944841112151 26.58903872530797 34-35 22.39539936675597 30.11546448577765 21.20293932906898 26.286196818397407 36-37 22.793740626321927 31.25200292266475 19.353039955903657 26.601216495109664 38-39 23.29094614862007 28.75427824281191 20.91483893297099 27.039936675597033 40-41 21.93056107472023 29.047666354744845 22.40260988834908 26.61916268218584 42-43 21.149741703093152 29.117528297291408 24.389188704157107 25.343541295458333 44-45 21.799329581725654 28.676244375793157 24.367236671751417 25.15718937072977 46-47 19.54067375113766 28.80699516734819 23.774692030611067 27.87763905090308 48-49 18.887400494802016 29.21110484418864 25.13812154696133 26.763373114048015 50-51 19.855533194036738 28.899770545179525 23.963928163976874 27.280768096806863 52-53 20.060151773467844 27.68984502185589 23.27844791119201 28.97155529348425 54-55 19.045391034597685 27.45606388842599 23.00060247913756 30.49794259783877 56-57 19.847937380324794 27.285706273985117 23.919818614451557 28.946537731238532 58-59 21.57356013895476 25.60824755483201 23.370742587583802 29.447449718629425 60-61 21.398905282588355 25.714963274410017 23.226532155721628 29.65959928728 62-63 21.49712861006781 25.900033328633143 23.40118701208804 29.20165104921101 64-65 21.66431385870654 25.65725062772703 23.305617736272925 29.37281777729351 66-67 22.7450659924017 27.30084106863306 22.32381062255743 27.630282316407808 68-69 22.174470403486843 27.612409063231098 22.16213184629683 28.050988686985225 70-71 21.836667318399048 27.21986993131168 23.196490901922825 27.746971848366446 72-73 23.46595907301386 26.17936281319752 23.56161881276168 26.79305930102694 74-75 24.494864920734376 25.2316754828609 23.416890877362224 26.8565687190425 76-77 25.12698570389337 24.796262053627224 23.518072829706163 26.55867941277324 78-79 25.632208587974013 24.945600097423096 24.56023407180467 24.861957242798223 80-81 25.83338140378539 25.692054274745068 24.275738522378397 24.198825799091146 82-83 24.97243978413301 25.018106420889364 25.10527361525939 24.904180179718242 84-85 25.324170602442074 24.872230867483896 25.295964434586534 24.507634095487493 86-87 26.09469664800978 24.81712993749169 24.795177518158816 24.292995896339715 88-89 26.32008282369835 24.411355564957628 24.61761349795505 24.650948113388978 90-91 26.95237515342437 24.484926756889276 24.424037712751126 24.13866037693523 92-93 26.99447754640239 25.440305898416344 23.81337760697951 23.751838948201755 94-95 26.193810996618645 26.261277864136794 23.082963414047853 24.461947725196712 96-97 25.724655679387233 26.825840671094696 22.241469101281137 25.208034548236935 98-99 25.23755195697946 28.19040690687322 22.116607966362963 24.455433169784353 100-101 24.82786799875017 29.74434572213721 21.963674819936386 23.464111459176234 102-103 23.959640242466197 31.740766912305496 21.95030500555211 22.349287839676197 104-105 23.189752694340203 33.561239957313575 21.587088958893485 21.661918389452737 106-107 22.518835665825964 34.714508670334986 21.153149746347435 21.61350591749161 108-109 22.520702208662883 36.43364397328582 20.716950173693455 20.328703644357844 110-111 22.503877658278963 38.29652228531874 19.679916934791247 19.519683121611056 112-113 22.196281898853034 39.45747513948488 19.32646671430998 19.01977624735211 114-115 21.757899442844714 40.295290699817166 19.441947367493345 18.504862489844776 116-117 21.53590519285742 40.99293689351502 19.06878517132199 18.402372742305573 118-119 21.44988607373699 41.875959073132485 18.930520212491324 17.743634640639204 120-121 22.086021298619723 41.59832585029435 18.407795179448723 17.9078576716372 122-123 22.66975395191206 41.83394357959507 18.274698949628647 17.22160351886422 124-125 22.723469182895002 43.626902228468175 17.404858462156195 16.244770126480628 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 5.5 2 5.5 3 6.0 4 9.0 5 8.0 6 8.5 7 10.0 8 10.0 9 14.5 10 21.5 11 26.5 12 32.5 13 41.0 14 59.5 15 82.5 16 112.0 17 147.5 18 184.0 19 259.0 20 356.5 21 469.0 22 657.5 23 890.5 24 1139.5 25 1414.5 26 1707.0 27 2078.0 28 2406.5 29 2645.5 30 2865.5 31 3001.5 32 3117.5 33 3074.0 34 2938.5 35 2815.0 36 2534.5 37 2206.0 38 1946.5 39 1776.0 40 1565.0 41 1370.0 42 1259.5 43 1211.5 44 1269.5 45 1444.5 46 1874.0 47 2734.5 48 4204.5 49 7367.0 50 12993.5 51 21015.5 52 29135.0 53 33385.5 54 31993.5 55 27475.5 56 22234.0 57 16682.0 58 12291.0 59 9134.0 60 6881.5 61 5122.0 62 3771.0 63 2958.0 64 2212.0 65 1709.0 66 1418.5 67 1168.0 68 921.0 69 785.5 70 706.0 71 563.5 72 472.0 73 396.5 74 319.5 75 233.0 76 161.0 77 129.5 78 105.5 79 75.5 80 52.5 81 39.5 82 32.5 83 23.5 84 12.5 85 15.0 86 17.5 87 8.0 88 1.0 89 1.5 90 2.5 91 2.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15382446065298483 2 0.012818705054415402 3 0.006088884900847316 4 0.010895899296253092 5 0.0032046762636038506 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.614028790811552E-4 28-29 0.007370755406288856 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.807014395405776E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0017625719449821178 66-67 1.6023381318019253E-4 68-69 0.004486546769045391 70-71 0.01762571944982118 72-73 1.6023381318019253E-4 74-75 0.007530989219469049 76-77 3.2046762636038506E-4 78-79 9.614028790811552E-4 80-81 6.409352527207701E-4 82-83 0.0 84-85 0.01778595326300137 86-87 0.0017625719449821178 88-89 0.017946187076181563 90-91 3.2046762636038506E-4 92-93 0.014421043186217328 94-95 0.012498237428055017 96-97 0.0052877158349463535 98-99 0.0022432733845226954 100-101 4.807014395405776E-4 102-103 1.6023381318019253E-4 104-105 3.2046762636038506E-4 106-107 9.614028790811552E-4 108-109 0.0 110-111 0.0 112-113 9.614028790811552E-4 114-115 0.004005845329504813 116-117 0.0 118-119 0.07034264398610451 120-121 0.0016023381318019253 122-123 0.003685377703144428 124-125 0.23442206868262166 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 312044.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.30052759357763 #Duplication Level Percentage of deduplicated Percentage of total 1 86.7062347516407 60.08787813941295 2 6.923092470404104 9.59547921556258 3 2.3330381828673707 4.850423309060113 4 1.2250490954205397 3.395861945627137 5 0.7223837166377329 2.5030786344002185 6 0.4750762907414297 1.9753822557348575 7 0.31971079417660925 1.5509288699660506 8 0.2415317008501105 1.3390619439589455 9 0.19911710231706464 1.241902821912926 >10 0.8112813887995264 8.750608873708856 >50 0.027756067752015758 1.372745069297966 >100 0.012952831617606313 1.7410972609427386 >500 0.002313005646001127 1.2618226512742807 >1k 4.626011292002254E-4 0.3337290091403776 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 1041 0.33360679904116086 No Hit ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 968 0.31021266231685274 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 942 0.3018805040314827 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 701 0.22464780607862994 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 697 0.2233659355731884 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 628 0.20125366935432182 No Hit GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 463 0.14837651100485827 No Hit GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT 374 0.11985489225878401 No Hit GGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAAT 336 0.10767712245708938 No Hit GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT 334 0.10703618720436861 No Hit ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA 317 0.10158823755624206 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.2046762636038506E-4 0.0 2 3.2046762636038506E-4 0.0 0.0 3.2046762636038506E-4 0.0 3 3.2046762636038506E-4 0.0 0.0 3.2046762636038506E-4 0.0 4 3.2046762636038506E-4 0.0 0.0 3.2046762636038506E-4 0.0 5 9.614028790811552E-4 0.0 0.0 3.2046762636038506E-4 0.0 6 0.0012818705054415402 0.0 0.0 3.2046762636038506E-4 0.0 7 0.0022432733845226954 0.0 0.0 3.2046762636038506E-4 0.0 8 0.0022432733845226954 0.0 0.0 3.2046762636038506E-4 0.0 9 0.0025637410108830805 0.0 0.0 3.2046762636038506E-4 0.0 10-11 0.004166079142685006 0.0 0.0 3.2046762636038506E-4 0.0 12-13 0.006088884900847316 0.0 0.0 9.614028790811552E-4 0.0 14-15 0.010415197856712514 0.0 0.0 0.0019228057581623104 0.0 16-17 0.013780107933496558 0.0 0.0 0.002403507197702888 0.0 18-19 0.015061978438938098 0.0 0.0 0.0028842086372434655 0.0 20-21 0.016023381318019253 0.0 0.0 0.006249118714027509 0.0 22-23 0.016984784197100408 0.0 0.0 0.01842688851572214 0.0 24-25 0.01778595326300137 0.0 0.0 0.045826870569535064 0.0 26-27 0.01890758995526272 0.0 0.0 0.11969465844560383 0.0 28-29 0.02034969427388445 0.0 0.0 0.368698004127623 0.0 30-31 0.023073669097947724 0.0 0.0 0.8947456127981951 0.0 32-33 0.023874838163848687 0.0 0.0 1.7016830959736446 0.0 34-35 0.025797643922010997 0.0 0.0 2.9119290869236387 0.0 36-37 0.02980348925151581 0.0 0.0 4.624988783633077 0.0 38-39 0.03092512594377716 0.0 0.0 6.749208444962889 0.0 40-41 0.033328633141480046 0.0 0.0 9.05433208137314 0.0 42-43 0.03525143889964236 0.0 0.0 10.913204548076553 0.0 44-45 0.03621284177872351 0.0 0.0 12.605113381446206 0.0 46-47 0.036373075591903704 0.0 0.0 14.609157682890874 0.0 48-49 0.0365333094050839 0.0 0.0 16.872460294061092 0.0 50-51 0.0365333094050839 0.0 0.0 18.924254271833462 0.0 52-53 0.03685377703144428 0.0 0.0 20.72800630680289 0.0 54-55 0.03749471228416505 0.0 0.0 22.574540769891424 0.0 56-57 0.03749471228416505 0.0 0.0 24.469465844560382 0.0 58-59 0.03797541372370563 0.0 0.0 26.328979246516518 0.0 60-61 0.0386163489764264 0.0 0.0 28.20451602979067 0.0 62-63 0.03877658278960659 0.0 0.0 29.93600261501583 0.0 64-65 0.03941751804232736 0.0 0.0 31.62518747356142 0.0 66-67 0.04037892092140852 0.0 0.0 33.52411839355988 0.0 68-69 0.04053915473458871 0.0 0.0 35.519029367653275 3.2046762636038506E-4 70-71 0.0406993885477689 0.0 0.0 37.6544653959057 3.2046762636038506E-4 72-73 0.04101985617412929 0.0 0.0 39.72532719744652 3.2046762636038506E-4 74-75 0.04101985617412929 0.0 0.0 41.565933009447384 3.2046762636038506E-4 76-77 0.04101985617412929 0.0 0.0 43.49418671725782 3.2046762636038506E-4 78-79 0.04118008998730948 0.0 0.0 45.38927202573996 3.2046762636038506E-4 80-81 0.04166079142685006 0.0 0.0 47.21257258591737 3.2046762636038506E-4 82-83 0.04166079142685006 0.0 0.0 49.15188242683724 3.2046762636038506E-4 84-85 0.04166079142685006 0.0 0.0 51.26857109894759 3.2046762636038506E-4 86-87 0.04166079142685006 0.0 0.0 52.97698401507479 3.2046762636038506E-4 88-89 0.04166079142685006 0.0 0.0 54.45369883734345 3.2046762636038506E-4 90-91 0.04166079142685006 0.0 0.0 56.30407891194832 3.2046762636038506E-4 92-93 0.04166079142685006 0.0 0.0 57.883824076091834 3.2046762636038506E-4 94-95 0.04198125905321044 0.0 0.0 59.312628988219615 3.2046762636038506E-4 96-97 0.04198125905321044 0.0 0.0 60.658272551306865 3.2046762636038506E-4 98-99 0.04198125905321044 0.0 0.0 61.843842535027115 3.2046762636038506E-4 100-101 0.04198125905321044 0.0 0.0 62.91244183512582 3.2046762636038506E-4 102-103 0.04198125905321044 0.0 0.0 63.9124610631834 3.2046762636038506E-4 104-105 0.04198125905321044 0.0 0.0 64.87738908615452 3.2046762636038506E-4 106-107 0.04198125905321044 0.0 0.0 65.76828908743639 3.2046762636038506E-4 108-109 0.04198125905321044 0.0 0.0 66.58484059940265 3.2046762636038506E-4 110-111 0.04198125905321044 0.0 0.0 67.38504826242453 3.2046762636038506E-4 112-113 0.04198125905321044 0.0 0.0 68.16330389304073 3.2046762636038506E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTTAG 25 0.002049021 71.4563 1 GTATTAG 30 0.004220465 59.54692 1 GTCCTAG 40 1.7630846E-4 59.54692 1 TAACAAC 185 0.0 57.937546 1 GTATGAG 110 3.6379788E-12 54.133564 1 TCCTAGG 45 3.1675538E-4 52.88818 2 GTGTACG 80 3.8109647E-8 52.103554 1 TGTTAGG 35 0.007791376 50.999313 2 GAGACGC 35 0.0077963155 50.99114 6 TGTACGG 85 6.182745E-8 48.99934 2 GTATAGG 125 1.0913936E-11 47.63753 1 AGTCTTG 50 5.33116E-4 47.59173 7 GTAACAA 90 9.633732E-8 46.314274 1 TATAAGG 80 2.2887052E-6 44.6244 2 GTCCAAG 55 8.485835E-4 43.30685 1 GGAAACA 350 0.0 40.832176 1 TATGAGG 140 1.884473E-9 38.249485 2 ATGAGGG 140 1.884473E-9 38.249485 3 GGAGGTA 140 1.886292E-9 38.243355 9 TGTATGG 80 1.1955641E-4 37.187 2 >>END_MODULE