FastQCFastQC Report
Thu 26 May 2016
SRR935918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312044
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT62001.9868992834343873No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT36801.179320865006217RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT21980.7043878427401263RNA PCR Primer, Index 17 (95% over 23bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA10490.33617054005204394No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT9890.31694248247042084No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT9030.2893822666034277No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA7960.2550922305828665No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC7540.24163259027573034No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC7180.23009575572675647No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC6510.20862442476061066No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG5940.19035777005806873TruSeq Adapter, Index 12 (95% over 23bp)
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA4560.1461332376203356No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT4110.13171219443411825No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT3880.1243414390278294RNA PCR Primer, Index 17 (96% over 26bp)
ATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAATTGCCC3700.11857302175334247No Hit
GTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.11024086346797246No Hit
GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT3390.10863852533617054No Hit
GGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAAT3390.10863852533617054No Hit
GTGGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCC3360.10767712245708938No Hit
GGTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.10575431669892707No Hit
GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT3250.10415197856712514No Hit
ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA3220.10319057568804399No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT359.059419E-568.124694
GGGCGAC551.7244929E-764.903067
CGAATAA150.004234515659.50401118-119
GTGTAAT150.004237204359.49447314-15
CTATACA859.495125E-1056.1929131
CTTTACA901.5752448E-953.0710831
CCTAGAC453.1388155E-452.985873
AGGGGCG803.8326107E-852.0660065
CTACGGG350.00773472951.0935173
TAGGGTA350.00779382450.995267
CGAGTTT255.333161E-447.59557716-17
GTGTAGG951.4432953E-743.9931371
GTCTAGG558.37578E-443.4217951
ACGCAGT558.522743E-443.2687037
AGTTGCT705.445795E-542.4960487
GTGTACG600.001285128439.8033141
TATTAGG758.087124E-539.73942
GGCGACA600.001307645339.6629838
GGGGCGA905.146687E-639.662986
AGTTAAT650.001919391736.6825262
Produced by FastQC (version 0.11.5)