##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935918_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 312044 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29955390906411 33.0 31.0 34.0 30.0 34.0 2 32.409365345912754 34.0 31.0 34.0 31.0 34.0 3 32.3954666649575 34.0 31.0 34.0 30.0 34.0 4 35.88830741818462 37.0 35.0 37.0 35.0 37.0 5 35.894486034020844 37.0 35.0 37.0 35.0 37.0 6 35.98889259207035 37.0 35.0 37.0 35.0 37.0 7 35.968927458948095 37.0 35.0 37.0 35.0 37.0 8 35.99895848021433 37.0 35.0 37.0 35.0 37.0 9 37.65774057504711 39.0 37.0 39.0 35.0 39.0 10-11 37.60339407263078 39.0 37.0 39.0 35.0 39.0 12-13 37.561625924549105 39.0 37.0 39.0 35.0 39.0 14-15 38.68454929433029 40.0 38.0 41.0 34.0 41.0 16-17 38.63544083526682 40.0 38.0 41.0 34.5 41.0 18-19 38.723083924061996 40.0 38.0 41.0 34.0 41.0 20-21 38.82667828895925 40.0 38.0 41.0 35.0 41.0 22-23 38.653824460652984 40.0 38.0 41.0 34.0 41.0 24-25 38.326085423850486 40.0 38.0 41.0 33.5 41.0 26-27 38.126802630398274 40.0 37.0 41.0 33.0 41.0 28-29 38.00432631295587 40.0 37.0 41.0 33.0 41.0 30-31 37.76620765020317 40.0 36.5 41.0 33.0 41.0 32-33 37.311395187858125 39.5 35.0 41.0 33.0 41.0 34-35 36.992283139557244 39.0 35.0 41.0 32.5 41.0 36-37 36.736487482534514 39.0 35.0 41.0 32.0 41.0 38-39 36.15413371191242 39.0 35.0 41.0 30.5 41.0 40-41 35.77289580956532 39.0 35.0 41.0 29.5 41.0 42-43 35.30764571662971 39.0 35.0 41.0 27.5 41.0 44-45 34.69034174667675 38.0 35.0 41.0 25.0 41.0 46-47 33.919504941610796 37.5 34.5 40.0 20.5 41.0 48-49 32.94943822025099 37.0 34.0 40.0 2.0 41.0 50-51 31.94498372024458 36.0 33.0 39.0 2.0 41.0 52-53 31.314141595416032 35.5 33.0 39.0 2.0 41.0 54-55 30.837253720629143 35.0 32.0 38.5 2.0 40.5 56-57 30.475062811654766 35.0 32.0 37.0 2.0 40.0 58-59 30.135394367460997 35.0 32.0 37.0 2.0 39.0 60-61 29.78487809411493 35.0 31.0 36.0 2.0 39.0 62-63 29.443544820602224 35.0 31.0 35.5 2.0 37.0 64-65 29.11668706977221 35.0 31.0 35.0 2.0 37.0 66-67 28.638714412070094 35.0 30.5 35.0 2.0 36.0 68-69 28.11353687300509 35.0 29.5 35.0 2.0 36.0 70-71 27.60559568522388 35.0 28.5 35.0 2.0 35.0 72-73 27.299376049531475 35.0 27.0 35.0 2.0 35.0 74-75 27.192314224917 35.0 27.0 35.0 2.0 35.0 76-77 27.09620758610965 35.0 27.0 35.0 2.0 35.0 78-79 27.12532046762636 35.0 27.0 35.0 2.0 35.0 80-81 27.096558498160515 35.0 27.0 35.0 2.0 35.0 82-83 27.02318423042904 35.0 27.0 35.0 2.0 35.0 84-85 26.963860865775338 35.0 27.0 35.0 2.0 35.0 86-87 26.92379440078963 35.0 27.0 35.0 2.0 35.0 88-89 26.85438912461063 35.0 25.5 35.0 2.0 35.0 90-91 26.747503557190655 34.5 25.0 35.0 2.0 35.0 92-93 26.6753646921588 34.0 25.0 35.0 2.0 35.0 94-95 26.63277774929177 34.0 25.0 35.0 2.0 35.0 96-97 26.600710156260014 34.0 25.0 35.0 2.0 35.0 98-99 26.517644947507403 34.0 24.5 35.0 2.0 35.0 100-101 26.41186819807463 34.0 24.0 35.0 2.0 35.0 102-103 25.688622437861326 33.0 21.5 35.0 2.0 35.0 104-105 26.0548993090718 34.0 21.5 35.0 2.0 35.0 106-107 25.91816218225635 34.0 19.5 35.0 2.0 35.0 108-109 25.606925625873274 34.0 12.0 35.0 2.0 35.0 110-111 25.150927433310688 34.0 2.0 35.0 2.0 35.0 112-113 24.54433188909256 34.0 2.0 35.0 2.0 35.0 114-115 23.84311186883901 33.5 2.0 35.0 2.0 35.0 116-117 23.15799855148633 33.0 2.0 35.0 2.0 35.0 118-119 22.571927356398454 33.0 2.0 35.0 2.0 35.0 120-121 21.904527887092847 33.0 2.0 35.0 2.0 35.0 122-123 21.16177045544859 32.5 2.0 35.0 2.0 35.0 124-125 19.130340592993296 27.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2793647647306159 1101 2 0.36375417849121305 1101 3 0.3233277385624618 1101 4 0.25690590158094295 1101 5 0.3675965669178467 1101 6 0.3718686927954664 1101 7 0.27495315689835564 1101 8 0.28549702547236677 1101 9 0.4879709238143448 1101 10-11 0.19263519383194705 1101 12-13 0.27080434936809183 1101 14-15 0.4380055957652331 1101 16-17 0.12844252535222012 1101 18-19 0.44008905913293717 1101 20-21 0.2560049920440619 1101 22-23 0.28478013505841204 1101 24-25 0.4733779636423847 1101 26-27 0.6137225808129401 1101 28-29 0.7382406407505258 1101 30-31 0.6699610144530652 1101 32-33 0.8792818739831034 1101 34-35 0.7270485940121247 1101 36-37 0.7258065456734428 1101 38-39 0.9765826607615509 1101 40-41 1.134729668927271 1101 42-43 1.1933026701684994 1101 44-45 1.4287823566562068 1101 46-47 1.3125548755221033 1101 48-49 1.3075006588392384 1101 50-51 1.3380902083039885 1101 52-53 0.9186535014781967 1101 54-55 1.0148064074947314 1101 56-57 0.855966822642614 1101 58-59 0.8891391406399016 1101 60-61 0.654008048047892 1101 62-63 0.8633548337964996 1101 64-65 0.7408575921505829 1101 66-67 0.4640171535390323 1101 68-69 0.3136718220553796 1101 70-71 0.2737877404242397 1101 72-73 0.3051524778442172 1101 74-75 0.22271660717744624 1101 76-77 0.31046971263083023 1101 78-79 0.1695113928929679 1101 80-81 0.26188739571331965 1101 82-83 0.06374554967699098 1101 84-85 -0.011475763383348436 1101 86-87 -0.0145389220578096 1101 88-89 -0.1286554414193617 1101 90-91 -0.1044345904285997 1101 92-93 -0.14663264650288355 1101 94-95 -0.03532220944965303 1101 96-97 0.06067682956661713 1101 98-99 0.19073455142080675 1101 100-101 0.023825529627277575 1101 102-103 0.12987493386636828 1101 104-105 0.2874307983205142 1101 106-107 -0.015585645040605556 1101 108-109 0.1467100294838133 1101 110-111 0.25956666247256877 1101 112-113 0.20098047644867734 1101 114-115 0.25251912956207434 1101 116-117 0.04502341494530171 1101 118-119 -0.16042375258056296 1101 120-121 -8.044830889026855E-4 1101 122-123 -0.04932025113107841 1101 124-125 0.1608852580111133 1102 1 0.11770027630641522 1102 2 0.11125697908867238 1102 3 0.16016959815917176 1102 4 0.2700453713344899 1102 5 0.26651833689544446 1102 6 0.3490905001143645 1102 7 0.44312903441888807 1102 8 0.23461950493464911 1102 9 0.25323417997565656 1102 10-11 0.4169280429730904 1102 12-13 0.355370723527912 1102 14-15 0.44151801174731276 1102 16-17 0.4707105425292184 1102 18-19 0.30395668422628574 1102 20-21 0.3269884685190405 1102 22-23 0.3788685405849179 1102 24-25 0.49309883742879634 1102 26-27 0.4033627040392318 1102 28-29 0.3978396885473927 1102 30-31 0.563479965162557 1102 32-33 0.5144923855589028 1102 34-35 0.5322203639897154 1102 36-37 0.8317974904083201 1102 38-39 0.9778896062432523 1102 40-41 0.8688012708690209 1102 42-43 0.7342730771886679 1102 44-45 0.864158570248442 1102 46-47 1.0533479113697481 1102 48-49 1.1381461965539472 1102 50-51 0.7610387706639656 1102 52-53 0.881989013053996 1102 54-55 0.9121178339323706 1102 56-57 0.7979011019555386 1102 58-59 0.736669947211972 1102 60-61 0.887017756785756 1102 62-63 0.8920726269256996 1102 64-65 1.0634164046972572 1102 66-67 0.8828114963321632 1102 68-69 1.1092515457297623 1102 70-71 1.1815080540911573 1102 72-73 1.3249083590988846 1102 74-75 1.2683780149444353 1102 76-77 1.2757189658048418 1102 78-79 1.234298547485949 1102 80-81 1.4475436690516297 1102 82-83 1.4310555758159005 1102 84-85 1.5157250394545905 1102 86-87 1.4638147934014896 1102 88-89 1.4295503091407582 1102 90-91 1.4763043005348067 1102 92-93 1.302839907642003 1102 94-95 1.2931878727012105 1102 96-97 1.447742736960194 1102 98-99 1.3740103169174454 1102 100-101 1.3864912316437064 1102 102-103 1.3672138995123753 1102 104-105 1.243274711688695 1102 106-107 1.1670716551834417 1102 108-109 0.9281934126055482 1102 110-111 0.9325035556764512 1102 112-113 0.513737197882584 1102 114-115 0.26395490252697584 1102 116-117 -0.2293417829635871 1102 118-119 -0.020592255194458176 1102 120-121 0.3001663906975054 1102 122-123 0.26396158232672207 1102 124-125 0.08910827892223949 1103 1 -0.002346682548683532 1103 2 0.1831152080689904 1103 3 0.15371834592303912 1103 4 0.22341075594987814 1103 5 0.02871645854123983 1103 6 0.04014595628609641 1103 7 0.1338490701970656 1103 8 0.1663279128058477 1103 9 0.06255886691662482 1103 10-11 0.06734731846504616 1103 12-13 0.03721888989642963 1103 14-15 0.08352941604069031 1103 16-17 0.2644325908297489 1103 18-19 0.1892988130277189 1103 20-21 0.2836353826514184 1103 22-23 0.33392779818778706 1103 24-25 0.4330697676613582 1103 26-27 0.3252488847190165 1103 28-29 0.5685183558103546 1103 30-31 0.6709318882394797 1103 32-33 0.6248960080991495 1103 34-35 0.7928196484262102 1103 36-37 0.7325857283331842 1103 38-39 0.5776212699284073 1103 40-41 0.5060832476132049 1103 42-43 0.7309691863121017 1103 44-45 0.5340713609461218 1103 46-47 0.28758874321232497 1103 48-49 0.39870634861119925 1103 50-51 0.3700895309501888 1103 52-53 0.4084536821058755 1103 54-55 0.40903196631161975 1103 56-57 0.33922489444211834 1103 58-59 0.20385465204202546 1103 60-61 0.40155264050668293 1103 62-63 0.5639588076054842 1103 64-65 0.5701136318886739 1103 66-67 0.5389776412337746 1103 68-69 0.5964049446564204 1103 70-71 0.619723617597419 1103 72-73 0.6475548260040718 1103 74-75 0.5523169684328089 1103 76-77 0.6591827404023398 1103 78-79 0.5027243077721728 1103 80-81 0.2951398228977844 1103 82-83 0.5477091983561486 1103 84-85 0.6615937782739145 1103 86-87 0.6604102764068571 1103 88-89 0.5607891284609714 1103 90-91 0.7261309999981371 1103 92-93 0.5961874031697327 1103 94-95 0.7099588923792055 1103 96-97 0.6847172450102832 1103 98-99 0.7409490467922204 1103 100-101 0.7625701672429912 1103 102-103 0.7468482912833956 1103 104-105 0.7440349979141025 1103 106-107 0.884843345702226 1103 108-109 0.6360981532137764 1103 110-111 0.7920965789322665 1103 112-113 0.38930741255163426 1103 114-115 0.5662581225627505 1103 116-117 0.9488335300954489 1103 118-119 0.8814705802259368 1103 120-121 0.5995849953486712 1103 122-123 0.012804381968940959 1103 124-125 0.6193352467218816 1104 1 -0.044562076943975626 1104 2 -0.07021424046626379 1104 3 -0.014708840600242468 1104 4 0.12341075594987672 1104 5 0.07812579265153374 1104 6 0.021159888584662667 1104 7 -0.00525671991788812 1104 8 0.039792976110398115 1104 9 0.02238828896462053 1104 10-11 -0.06376726542010402 1104 12-13 -0.04220845555040853 1104 14-15 -0.061167834581752345 1104 16-17 0.15911130037046206 1104 18-19 0.09781181827219854 1104 20-21 0.033502946353287655 1104 22-23 0.1297692984261687 1104 24-25 0.14934883742879634 1104 26-27 0.07086259452460553 1104 28-29 0.349924299551418 1104 30-31 0.06717334609824377 1104 32-33 0.2492616382310615 1104 34-35 -0.04533789779406305 1104 36-37 -0.09623770759413475 1104 38-39 0.049293145756131196 1104 40-41 -0.22854354689862788 1104 42-43 -0.5429276193243879 1104 44-45 -0.566863639318754 1104 46-47 -0.3630787357303049 1104 48-49 -0.12056843022018882 1104 50-51 0.07815755023290905 1104 52-53 0.12006834545280753 1104 54-55 0.35142482534642383 1104 56-57 0.32111820376033506 1104 58-59 0.2387781038617014 1104 60-61 0.11964873628461703 1104 62-63 -0.06514274454793068 1104 64-65 0.12723605600267263 1104 66-67 0.21481331427503747 1104 68-69 0.2957056810022358 1104 70-71 0.3344452364987269 1104 72-73 0.48761150873967907 1104 74-75 0.48431622678953445 1104 76-77 0.40880662131561607 1104 78-79 0.6454164728407754 1104 80-81 0.639900642943342 1104 82-83 0.7850588831577632 1104 84-85 0.6995261827873414 1104 86-87 0.7185868934540594 1104 88-89 0.7396628901815845 1104 90-91 0.5532301153236645 1104 92-93 0.4908820050662186 1104 94-95 0.6189062886815861 1104 96-97 0.4063626335783823 1104 98-99 0.5116647325473735 1104 100-101 0.4839400883109555 1104 102-103 0.49080389863625484 1104 104-105 0.7743549640104099 1104 106-107 0.9030400930667426 1104 108-109 0.9767905301804554 1104 110-111 1.1205730317584575 1104 112-113 0.9776795055748906 1104 114-115 1.1348919097111327 1104 116-117 1.4817148141977938 1104 118-119 1.0127467363948242 1104 120-121 0.9986288052760912 1104 122-123 1.0687216357698617 1104 124-125 1.0588356970052963 1105 1 -0.03879821116117199 1105 2 -0.06337028970994396 1105 3 -0.044557065626477765 1105 4 0.11666918291616923 1105 5 0.07849435245292113 1105 6 0.18271193485939108 1105 7 0.08490734038259262 1105 8 0.03468432775228081 1105 9 0.019731587062956635 1105 10-11 0.04690049327560786 1105 12-13 0.09801063277597422 1105 14-15 0.1100384309348641 1105 16-17 0.1605522668161612 1105 18-19 0.29131083288661586 1105 20-21 0.20120789375672388 1105 22-23 0.25592936599547045 1105 24-25 0.4482252419231756 1105 26-27 0.6500594412907432 1105 28-29 0.6604180161186974 1105 30-31 0.6726172814979066 1105 32-33 0.1878042913497424 1105 34-35 0.5390699750528043 1105 36-37 0.6814490677635519 1105 38-39 0.691718474004702 1105 40-41 0.6371650349139699 1105 42-43 0.7233261238610922 1105 44-45 1.0261669972147374 1105 46-47 1.2926710531156473 1105 48-49 1.4089624238659653 1105 50-51 1.106201367898187 1105 52-53 0.8226815368223654 1105 54-55 0.7506441952115388 1105 56-57 0.8615138936582163 1105 58-59 0.916331788179992 1105 60-61 1.1193211275722739 1105 62-63 1.1651738585592355 1105 64-65 1.2793898273420297 1105 66-67 1.0149771186312115 1105 68-69 1.341322634753098 1105 70-71 1.3424306988621169 1105 72-73 1.3291461508015665 1105 74-75 1.4274018913489321 1105 76-77 1.19021226744702 1105 78-79 1.17479120089995 1105 80-81 1.31367914874048 1105 82-83 1.354437706325843 1105 84-85 1.2741493047959906 1105 86-87 1.2923935531249136 1105 88-89 1.3208100325634007 1105 90-91 0.9665673731363604 1105 92-93 1.269861299171822 1105 94-95 1.4280409409812442 1105 96-97 1.4371604119818002 1105 98-99 1.1856838259926405 1105 100-101 1.1902158427068024 1105 102-103 1.03897005768005 1105 104-105 1.5137371144544716 1105 106-107 1.355211780507556 1105 108-109 1.2389594022338954 1105 110-111 1.5329249039910557 1105 112-113 1.260825572990619 1105 114-115 1.29745749543968 1105 116-117 0.966941708825523 1105 118-119 1.1126699531028663 1105 120-121 1.1066270648548056 1105 122-123 1.119349979042365 1105 124-125 0.8797872979369288 1106 1 0.18848019083633005 1106 2 0.22797706455875755 1106 3 0.21599224773182613 1106 4 0.18751332005243881 1106 5 0.17169996082706973 1106 6 0.2892614402853013 1106 7 0.24601364980350837 1106 8 0.3852605305756782 1106 9 0.43432392356540106 1106 10-11 0.31930517972523376 1106 12-13 0.4150662363484301 1106 14-15 0.451801131405432 1106 16-17 0.24782058526425743 1106 18-19 0.3571083936279962 1106 20-21 0.48871389586947345 1106 22-23 0.43429054913193 1106 24-25 0.29998986306981834 1106 26-27 0.2395005245520494 1106 28-29 0.338678363761062 1106 30-31 0.5841797514873406 1106 32-33 0.5586964453879659 1106 34-35 0.44675027851963023 1106 36-37 0.5084801827160135 1106 38-39 0.6872913156449698 1106 40-41 0.9140737067664517 1106 42-43 0.9798660259066097 1106 44-45 1.0134641258039991 1106 46-47 0.9661417147885558 1106 48-49 0.7544656409983901 1106 50-51 0.40914240314259587 1106 52-53 0.5467860216010081 1106 54-55 0.5007930476075835 1106 56-57 0.5585848626392966 1106 58-59 0.29089003268205715 1106 60-61 0.42761604738404557 1106 62-63 0.3606890799171545 1106 64-65 0.39488007991093355 1106 66-67 0.2515347869304527 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-1.4510673625301678 2216 58-59 -1.2545343705009664 2216 60-61 -1.4467945037506418 2216 62-63 -1.5637877842148846 2216 64-65 -1.7326619183207228 2216 66-67 -2.141811809002352 2216 68-69 -2.1735273372646375 2216 70-71 -2.4669777991360746 2216 72-73 -2.0487607758849045 2216 74-75 -1.9456425789252982 2216 76-77 -2.04977653964756 2216 78-79 -2.3062260620041783 2216 80-81 -2.2540938935773127 2216 82-83 -2.266663072872575 2216 84-85 -2.267841310383311 2216 86-87 -2.2748521311490535 2216 88-89 -2.253418302707164 2216 90-91 -2.1127623440275265 2216 92-93 -2.3353950614119334 2216 94-95 -2.4813847768714403 2216 96-97 -2.3257523264055635 2216 98-99 -2.82904560704365 2216 100-101 -2.631067897445593 2216 102-103 -2.5546887309031874 2216 104-105 -2.5059516366755794 2216 106-107 -2.248632552007834 2216 108-109 -2.3156081641701682 2216 110-111 -2.480571156967226 2216 112-113 -2.189945015497905 2216 114-115 -2.2688884514370713 2216 116-117 -2.249014638843338 2216 118-119 -1.9661281010535774 2216 120-121 -1.7750251801837216 2216 122-123 -1.7318984604083205 2216 124-125 -1.826909072978733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 19.0 5 74.0 6 220.0 7 584.0 8 902.0 9 1366.0 10 2177.0 11 4127.0 12 6131.0 13 6446.0 14 8489.0 15 11154.0 16 5112.0 17 1903.0 18 1791.0 19 2258.0 20 3498.0 21 5242.0 22 4800.0 23 1506.0 24 1831.0 25 2402.0 26 3051.0 27 3765.0 28 4536.0 29 5507.0 30 7297.0 31 9918.0 32 13455.0 33 18248.0 34 24395.0 35 36513.0 36 82771.0 37 28715.0 38 1823.0 39 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.91729709595797 21.014809201484773 12.208279281246387 14.859614421310866 2 19.702602230483272 21.579925650557623 37.739071913857195 20.97840020510191 3 29.121534142620913 23.156349745548706 29.333042776018765 18.389073335811617 4 15.409046141803518 23.84501127639848 27.71523805551902 33.03070452627898 5 14.712519874852543 34.47197004667385 28.107529363491818 22.707980714981794 6 29.60597368884901 36.723124008524685 9.698591504158188 23.972310798468122 7 25.7329094614862 41.59445462819346 12.343772032149312 20.328863878171028 8 34.85822512209816 27.461511838074117 12.950096781223161 24.730166258604555 9 32.50054479496482 14.44860340208432 18.163143659227547 34.88770814372332 10-11 29.37742754226968 24.966030431605798 18.892688210636962 26.763853815487558 12-13 33.204932637704935 26.228031944212997 20.136903769981156 20.430131648100907 14-15 34.67363576931458 19.346149901936908 25.067137967722502 20.913076361026008 16-17 30.25038135647537 20.66359231390445 26.46453705246696 22.621489277153223 18-19 28.692588224737538 20.816134904051992 27.011094589224598 23.480182281985872 20-21 34.19613900603761 17.576687902988038 27.277403186730076 20.949769904244274 22-23 29.85027752496443 21.320871415569602 27.083199805155683 21.74565125431029 24-25 25.371261745138508 22.54425657920037 29.03356577918499 23.050915896476138 26-27 22.675007370755406 22.16241940239197 31.417364217866712 23.745209008985913 28-29 23.405833792670265 19.967536629449693 33.42589506608042 23.200734511799617 30-31 26.446911333017137 20.2740639140634 30.485284126597534 22.793740626321927 32-33 21.18883875350912 21.46588301649767 32.27317301406212 25.072105215931085 34-35 21.59455076848137 21.1072797426004 33.38695824947764 23.91121123944059 36-37 21.8531681429542 21.97895168630065 31.109394828934384 25.058485341810773 38-39 23.116291292253656 17.23282613990335 33.06168360872185 26.589198959121152 40-41 21.85509094871236 17.40043070848983 34.30429682993424 26.44018151286357 42-43 22.846137083231852 17.2283395931343 35.522394277730065 24.403129045903782 44-45 22.396200535821873 18.124367076437938 34.24356821473894 25.235864173001243 46-47 21.317987206932358 17.35155939546987 32.55053774467703 28.77991565292074 48-49 20.445674327979386 18.588243965594593 32.507595082744736 28.458486623681278 50-51 20.69756188229865 18.83340169976029 31.31545551268411 29.15358090525695 52-53 20.543577187832483 18.667880170745153 30.749349450718487 30.039193190703877 54-55 18.777480098960403 19.593390675673945 28.3822153286075 33.24691389675815 56-57 19.84223378754278 20.709899885913526 27.06349104613452 32.384375280409174 58-59 19.30865518965274 20.69692094704593 26.932419786953123 33.0620040763482 60-61 19.852648985399497 22.410301111381727 24.054940969863225 33.68210893335555 62-63 18.153209162810374 24.07609183320301 24.153164297342684 33.61753470664394 64-65 17.84860468395483 23.580809116663033 24.461293920088192 34.109292279293946 66-67 18.75841227519196 24.666072733332477 24.09628129366371 32.47923369781185 68-69 18.59561472100088 24.6804937765187 24.07753393752163 32.64635756495879 70-71 18.55139018856315 24.287279998974505 24.749073848559817 32.412255963902524 72-73 19.401590801297253 23.133276076450755 24.946802374024177 32.51833074822781 74-75 19.66677776211047 22.888438809911424 24.638192049839127 32.80659137813898 76-77 19.30785402058684 23.188877209624284 25.12273910089603 32.38052966889285 78-79 19.2402353514248 24.35954544871877 25.568990270602864 30.83122892925357 80-81 19.01751035110433 25.40731435310405 24.876940431477614 30.698234864314006 82-83 18.57222058427658 24.20844496288985 25.479740036661497 31.73959441617208 84-85 18.989629667610977 23.450218558921176 25.592224173513994 31.967927599953853 86-87 19.536988373434518 23.70787453051493 25.14292856135673 31.612208534693824 88-89 19.41521067541757 24.179442642704235 24.685140557100922 31.720206124777274 90-91 19.61854738434323 23.459031418646088 24.522022535283487 32.400398661727195 92-93 19.426106574713824 23.313058414838935 24.419633128661342 32.841201881785906 94-95 19.320031790388533 23.199292407480996 24.30154080834754 33.179134993782924 96-97 19.450141646690852 23.55260796554332 23.96793400930638 33.02931637845945 98-99 19.68167950673623 24.37540859622361 23.744247606106832 32.19866429093333 100-101 20.361551576059785 25.178660701695915 23.032008306520876 31.42777941572342 102-103 19.97138224096602 26.461652843829714 22.498429708630834 31.068535206573433 104-105 19.792721539270104 28.43044570637474 22.042724743946366 29.73410801040879 106-107 19.617265513837793 30.376164899821823 21.432073681916652 28.574495904423735 108-109 19.027925548961043 31.70097806719565 21.051518375613696 28.219578008229607 110-111 18.649192579227865 33.10985985905789 20.931410094121645 27.30953746759261 112-113 18.88038045977564 34.07537731117616 20.870487608298202 26.17375462074999 114-115 18.484429930682186 34.65506135411693 20.95125320070119 25.909255514499698 116-117 17.898279011633498 35.116815690798965 21.22103643880396 25.763868858763576 118-119 18.295659029254402 35.17386476348271 21.54493756379421 24.985538643468676 120-121 18.317857343150372 35.35608130302763 21.599621846383105 24.72643950743889 122-123 17.821490262435145 35.68480222023529 21.782533593557215 24.711173923772346 124-125 17.05156869816084 37.1424450054093 22.286973594582683 23.519012701847178 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 2.0 6 3.0 7 4.5 8 4.5 9 8.5 10 12.5 11 12.0 12 14.5 13 17.5 14 17.5 15 19.0 16 26.5 17 37.5 18 47.0 19 60.5 20 92.5 21 135.5 22 212.0 23 302.0 24 445.5 25 656.0 26 877.0 27 1116.0 28 1436.5 29 1883.5 30 2319.5 31 2725.0 32 3072.0 33 3298.5 34 3466.0 35 3553.5 36 3444.5 37 3321.5 38 3193.0 39 2972.5 40 2786.0 41 2667.0 42 2635.5 43 2671.0 44 2730.0 45 2790.0 46 3018.0 47 3531.5 48 4377.5 49 6178.5 50 10225.0 51 17365.0 52 26094.0 53 32087.5 54 32333.5 55 28909.0 56 24305.5 57 18824.0 58 13684.5 59 9749.0 60 6842.5 61 4748.5 62 3420.5 63 2535.5 64 1812.0 65 1379.0 66 1121.5 67 933.5 68 705.5 69 566.0 70 516.0 71 443.0 72 390.5 73 284.5 74 195.5 75 137.0 76 79.5 77 60.0 78 52.0 79 33.0 80 10.0 81 2.5 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1974080578379972 2 0.0012818705054415402 3 0.0 4 0.10607478432528746 5 0.029483021625155426 6 0.0028842086372434655 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 3.2046762636038506E-4 112-113 1.6023381318019253E-4 114-115 9.614028790811552E-4 116-117 0.004486546769045391 118-119 0.0017625719449821178 120-121 4.807014395405776E-4 122-123 0.0016023381318019253 124-125 0.026118111548371382 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 312044.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.466046428782846 #Duplication Level Percentage of deduplicated Percentage of total 1 83.18549581718732 49.46712556466183 2 8.525139008531557 10.139126241863309 3 2.9549996368826976 5.271664368117089 4 1.5414635701515478 3.6665897692363725 5 0.9167314131317572 2.725719638800839 6 0.6169150987278118 2.2011301142139126 7 0.4500683893739836 1.873465141704858 8 0.31531189979489666 1.500028165820084 9 0.233037990596108 1.247206317161258 >10 1.192520938017123 11.626949453955508 >50 0.043033850459977985 1.8310023853403106 >100 0.019365232706990088 2.5219466816951446 >500 0.0037654619152480734 1.7289601489804993 >1k 0.0016137693922491743 2.215819972615711 >5k 5.379231307497248E-4 1.9832660358333203 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 6200 1.9868992834343873 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 3680 1.179320865006217 RNA PCR Primer, Index 17 (95% over 21bp) ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 2198 0.7043878427401263 RNA PCR Primer, Index 17 (95% over 23bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 1049 0.33617054005204394 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 989 0.31694248247042084 No Hit GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 903 0.2893822666034277 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 796 0.2550922305828665 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 754 0.24163259027573034 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 718 0.23009575572675647 No Hit TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC 651 0.20862442476061066 No Hit CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG 594 0.19035777005806873 TruSeq Adapter, Index 12 (95% over 23bp) GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 456 0.1461332376203356 No Hit TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT 411 0.13171219443411825 No Hit CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT 388 0.1243414390278294 RNA PCR Primer, Index 17 (96% over 26bp) ATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAATTGCCC 370 0.11857302175334247 No Hit GTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 344 0.11024086346797246 No Hit GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT 339 0.10863852533617054 No Hit GGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAAT 339 0.10863852533617054 No Hit GTGGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCC 336 0.10767712245708938 No Hit GGTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 330 0.10575431669892707 No Hit GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT 325 0.10415197856712514 No Hit ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA 322 0.10319057568804399 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.2046762636038506E-4 0.0 0.0 0.0 0.0 3 3.2046762636038506E-4 0.0 0.0 0.0 0.0 4 3.2046762636038506E-4 0.0 0.0 0.0 0.0 5 3.2046762636038506E-4 0.0 0.0 0.0 0.0 6 3.2046762636038506E-4 0.0 0.0 0.0 0.0 7 0.0016023381318019253 0.0 0.0 0.0 0.0 8 0.0016023381318019253 0.0 0.0 0.0 0.0 9 0.0016023381318019253 0.0 0.0 0.0 0.0 10-11 0.002083039571342503 0.0 0.0 0.0 0.0 12-13 0.007210521593108664 0.0 0.0 0.0 0.0 14-15 0.012818705054415402 0.0 0.0 3.2046762636038506E-4 0.0 16-17 0.013459640307136173 0.0 0.0 6.409352527207701E-4 0.0 18-19 0.013619874120316365 0.0 0.0 8.011690659009627E-4 0.0 20-21 0.014260809373037135 0.0 0.0 0.004166079142685006 0.0 22-23 0.015061978438938098 0.0 0.0 0.016664316570740023 0.0 24-25 0.015702913691658868 0.0 0.0 0.04374383099819256 0.0 26-27 0.016343848944379638 0.0 0.0 0.11681044980836036 0.0 28-29 0.0171450180102806 0.0 0.0 0.3675763674353617 0.0 30-31 0.017946187076181563 0.0 0.0 0.892181871787312 0.0 32-33 0.018106420889361756 0.0 0.0 1.7024842650395458 0.0 34-35 0.019388291394803296 0.0 0.0 2.9124097883631794 0.0 36-37 0.01954852520798349 0.0 0.0 4.628033226083501 0.0 38-39 0.01970875902116368 0.0 0.0 6.754496160797836 0.0 40-41 0.02099062952660522 0.0 0.0 9.058978861955366 0.0 42-43 0.021952032405686377 0.0 0.0 10.91977413441694 0.0 44-45 0.022592967658407147 0.0 0.0 12.616489982182 0.0 46-47 0.02275320147158734 0.0 0.0 14.619412646934407 0.0 48-49 0.02275320147158734 0.0 0.0 16.883195959544167 0.0 50-51 0.022913435284767532 0.0 0.0 18.9401174193383 0.0 52-53 0.023073669097947724 0.0 0.0 20.74851623488995 0.0 54-55 0.023073669097947724 0.0 0.0 22.59633256848393 0.0 56-57 0.023073669097947724 0.0 0.0 24.4992693338119 0.0 58-59 0.023554370537488302 0.0 0.0 26.36487162066888 0.0 60-61 0.024355539603389265 0.0 0.0 28.258514824832396 0.0 62-63 0.024836241042929842 0.0 0.0 30.021887938880415 0.0 64-65 0.024996474856110035 0.0 0.0 31.736870441348017 0.0 66-67 0.02531694248247042 0.0 0.0 33.65358731460948 0.0 68-69 0.025477176295650612 0.0 0.0 35.67541756931715 0.0 70-71 0.025637410108830805 0.0 0.0 37.829921421338014 0.0 72-73 0.02595787773519119 0.0 0.0 39.919370345207724 0.0 74-75 0.02595787773519119 0.0 0.0 41.77920421479023 0.0 76-77 0.02595787773519119 0.0 0.0 43.72764738306137 0.0 78-79 0.026118111548371382 0.0 0.0 45.6429221520042 0.0 80-81 0.02659881298791196 0.0 0.0 47.48625193882914 0.0 82-83 0.02659881298791196 0.0 0.0 49.4539231646819 0.0 84-85 0.02723974824063273 0.0 0.0 51.59576854546154 0.0 86-87 0.027560215866993115 0.0 0.0 53.32789606593941 0.0 88-89 0.028361384932894078 0.0 0.0 54.83265180551461 0.0 90-91 0.02852161874607427 0.0 0.0 56.70290087295382 0.0 92-93 0.028842086372434655 0.0 0.0 58.30059222417351 0.0 94-95 0.02980348925151581 0.0 0.0 59.745580751432485 0.0 96-97 0.030123956877876196 0.0 0.0 61.102280447629184 0.0 98-99 0.030123956877876196 0.0 0.0 62.29986796733794 0.0 100-101 0.030123956877876196 0.0 0.0 63.3857725192601 0.0 102-103 0.030123956877876196 0.0 0.0 64.40501980489931 0.0 104-105 0.030123956877876196 0.0 0.0 65.38260629911167 0.0 106-107 0.030123956877876196 0.0 0.0 66.28808757739293 0.0 108-109 0.030123956877876196 0.0 0.0 67.11553498865544 0.0 110-111 0.030123956877876196 0.0 0.0 67.92439527758907 0.0 112-113 0.030123956877876196 0.0 0.0 68.70745792260065 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGACT 35 9.059419E-5 68.12469 4 GGGCGAC 55 1.7244929E-7 64.90306 7 CGAATAA 15 0.0042345156 59.50401 118-119 GTGTAAT 15 0.0042372043 59.494473 14-15 CTATACA 85 9.495125E-10 56.192913 1 CTTTACA 90 1.5752448E-9 53.071083 1 CCTAGAC 45 3.1388155E-4 52.98587 3 AGGGGCG 80 3.8326107E-8 52.066006 5 CTACGGG 35 0.007734729 51.093517 3 TAGGGTA 35 0.007793824 50.99526 7 CGAGTTT 25 5.333161E-4 47.595577 16-17 GTGTAGG 95 1.4432953E-7 43.993137 1 GTCTAGG 55 8.37578E-4 43.421795 1 ACGCAGT 55 8.522743E-4 43.268703 7 AGTTGCT 70 5.445795E-5 42.496048 7 GTGTACG 60 0.0012851284 39.803314 1 TATTAGG 75 8.087124E-5 39.7394 2 GGCGACA 60 0.0013076453 39.662983 8 GGGGCGA 90 5.146687E-6 39.66298 6 AGTTAAT 65 0.0019193917 36.682526 2 >>END_MODULE