FastQCFastQC Report
Thu 26 May 2016
SRR935917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866167
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC64200.22399253079112275No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT63280.22078266897916274No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT57000.19887187313230528No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT54640.19063787978858177No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT50840.17737975491309474No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG45120.15742278799525639No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA29030.10128509608826003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62050.057.6135031
GAGTAGT20500.049.7000731
GTATCAA105100.049.54771
GATTGTG63000.044.0938849
AGGGTAA43150.042.1849756
GAGTAAG18350.041.5612561
GGGGTAA64650.040.85239
GATAGCG11600.038.9724439
CGGGATT16800.038.9493836
TATCAAC133150.038.874382
GGGATTG72750.038.1857267
AGTAGTA19350.037.818962
TGGGGTA63800.037.667158
GTGGGGT66400.035.476797
ATCAACG147900.034.8341183
TCAACGC148800.034.5034834
GAGTAGG30300.034.215381
GAGTTAG17600.034.191921
TACGCGG1408.083043E-833.9951554
GAGTCAG64450.033.835531
Produced by FastQC (version 0.11.5)