##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935917_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2866167 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.287829355372523 31.0 31.0 34.0 30.0 34.0 2 31.45471390885458 33.0 31.0 34.0 28.0 34.0 3 31.61096998186079 34.0 31.0 34.0 28.0 34.0 4 34.98437634652831 37.0 35.0 37.0 32.0 37.0 5 34.95934186668118 37.0 35.0 37.0 32.0 37.0 6 35.08357852141902 37.0 35.0 37.0 32.0 37.0 7 35.03373355425556 37.0 35.0 37.0 32.0 37.0 8 35.031552941611565 37.0 35.0 37.0 32.0 37.0 9 36.69745761499592 39.0 38.0 39.0 33.0 39.0 10-11 36.60223270311883 39.0 37.0 39.0 33.0 39.0 12-13 36.493572775068586 39.0 37.0 39.0 32.0 39.0 14-15 37.87867489926442 40.0 38.0 41.0 32.5 41.0 16-17 37.91039653307013 40.0 38.0 41.0 32.5 41.0 18-19 37.92615224444354 40.0 38.0 41.0 32.0 41.0 20-21 37.96131558279751 40.0 38.0 41.0 32.5 41.0 22-23 37.85997187184138 40.0 38.0 41.0 32.0 41.0 24-25 37.80665310151153 40.0 38.0 41.0 32.0 41.0 26-27 37.74692524894746 40.0 38.0 41.0 32.0 41.0 28-29 37.65009558061341 40.0 38.0 41.0 32.0 41.0 30-31 37.584002258068004 40.0 38.0 41.0 32.0 41.0 32-33 37.50241943334076 40.0 38.0 41.0 31.5 41.0 34-35 37.36633088720929 40.0 37.0 41.0 31.0 41.0 36-37 37.35195855649723 40.0 37.0 41.0 31.5 41.0 38-39 37.385840566861596 40.0 37.0 41.0 31.0 41.0 40-41 37.31348173361845 40.0 37.0 41.0 31.0 41.0 42-43 37.177825821035555 40.0 37.0 41.0 31.0 41.0 44-45 37.032230501572315 40.0 36.0 41.0 31.0 41.0 46-47 36.895446601680916 40.0 36.0 41.0 30.5 41.0 48-49 36.72058292486098 40.0 35.0 41.0 30.0 41.0 50-51 36.5153375570928 40.0 35.0 41.0 30.0 41.0 52-53 36.32872875167428 39.0 35.0 41.0 30.0 41.0 54-55 36.09858950996226 39.0 35.0 41.0 30.0 41.0 56-57 35.84228762664562 39.0 35.0 41.0 30.0 41.0 58-59 35.5333661646373 38.0 35.0 40.5 29.0 41.0 60-61 35.25917976865968 37.0 35.0 40.0 29.0 41.0 62-63 34.92793354330016 37.0 34.5 40.0 28.5 41.0 64-65 34.611781693111396 36.0 34.0 39.0 28.5 41.0 66-67 34.36602943931739 36.0 34.0 39.0 28.5 41.0 68-69 34.159918106656036 35.0 34.0 39.0 29.0 41.0 70-71 33.85325070032556 35.0 34.0 37.5 29.0 40.0 72-73 33.55244861866039 35.0 34.0 37.0 28.5 39.0 74-75 33.244738530588066 35.0 34.0 36.5 28.0 39.0 76-77 32.97733244434117 35.0 34.0 36.0 28.0 39.0 78-79 32.72131962303662 35.0 34.0 36.0 27.5 37.0 80-81 32.4797438530274 35.0 33.5 35.0 27.0 37.0 82-83 32.29153378013214 35.0 33.0 35.0 27.0 36.5 84-85 32.1175470584931 35.0 33.0 35.0 27.0 36.0 86-87 31.9689977939178 35.0 33.0 35.0 26.0 36.0 88-89 31.822795740792493 35.0 33.0 35.0 26.0 36.0 90-91 31.69203033179853 35.0 33.0 35.0 25.0 35.0 92-93 31.560352728923334 35.0 33.0 35.0 25.0 35.0 94-95 31.438933949068563 35.0 33.0 35.0 24.5 35.0 96-97 31.33970787466327 35.0 33.0 35.0 24.0 35.0 98-99 31.197886934013265 35.0 33.0 35.0 24.0 35.0 100-101 31.091584509904692 35.0 33.0 35.0 23.0 35.0 102-103 30.398358330132194 34.0 31.5 35.0 20.0 35.0 104-105 30.890803641239327 35.0 32.5 35.0 21.0 35.0 106-107 30.93078351680136 35.0 33.0 35.0 20.0 35.0 108-109 30.84982225390216 35.0 33.0 35.0 20.0 35.0 110-111 30.735011079256722 35.0 32.5 35.0 19.0 35.0 112-113 30.604299226109298 35.0 32.0 35.0 18.5 35.0 114-115 30.435649597528688 35.0 32.0 35.0 16.0 35.0 116-117 30.281578672840766 35.0 32.0 35.0 4.5 35.0 118-119 30.03060027555966 35.0 32.0 35.0 2.0 35.0 120-121 29.78134037549103 35.0 32.0 35.0 2.0 35.0 122-123 29.401954247606646 35.0 31.0 35.0 2.0 35.0 124-125 26.69618814953909 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11193513079132345 1101 2 0.3327258362857499 1101 3 0.2467038489832234 1101 4 0.20770611373121994 1101 5 0.2023857328794918 1101 6 0.19282775832922283 1101 7 0.18707187321331276 1101 8 0.19234150087627455 1101 9 0.21681751862524123 1101 10-11 0.3072624576126799 1101 12-13 0.23385573854277908 1101 14-15 0.3387763733121929 1101 16-17 0.3118699793550874 1101 18-19 0.35168849840006544 1101 20-21 0.3208817184657917 1101 22-23 0.34212935088817886 1101 24-25 0.3525055522028211 1101 26-27 0.4086998667379831 1101 28-29 0.39631346207580975 1101 30-31 0.3407396996951064 1101 32-33 0.3170519341927047 1101 34-35 0.14045902565848678 1101 36-37 0.12597199077031007 1101 38-39 0.1998942883660817 1101 40-41 0.17132697674309583 1101 42-43 0.20267930620657637 1101 44-45 0.25135673335955744 1101 46-47 0.295336219617063 1101 48-49 0.21781853976595045 1101 50-51 0.2681044006387765 1101 52-53 0.3376654117257658 1101 54-55 0.2809447891951393 1101 56-57 0.2286940799964583 1101 58-59 0.15623791452616587 1101 60-61 0.25946866952294556 1101 62-63 0.38020647100982075 1101 64-65 0.30883010528190624 1101 66-67 0.2201437922132996 1101 68-69 0.3126565214655983 1101 70-71 0.1807005822620411 1101 72-73 0.2718148966695253 1101 74-75 0.29366394226597947 1101 76-77 0.34315581306692877 1101 78-79 0.3193902153601442 1101 80-81 0.29876860700629493 1101 82-83 0.21405070450785502 1101 84-85 0.15509860004748788 1101 86-87 0.1316064282142939 1101 88-89 0.15034454178960743 1101 90-91 0.10390362435154898 1101 92-93 -0.02810853980636807 1101 94-95 -0.022568468036396894 1101 96-97 0.030695659755366478 1101 98-99 -0.02643869664749232 1101 100-101 0.11241693544420173 1101 102-103 0.09986156269008717 1101 104-105 0.07073146883140424 1101 106-107 0.14188919385166088 1101 108-109 0.12910713859126943 1101 110-111 0.06215028900105324 1101 112-113 -0.004254403221221281 1101 114-115 -0.0512170657463642 1101 116-117 -0.0749222829640459 1101 118-119 -0.16993697700519306 1101 120-121 -0.3094253079335303 1101 122-123 -0.1834727394426814 1101 124-125 0.006929487820368507 1102 1 0.07097653205499554 1102 2 0.1430562703619458 1102 3 0.2018399647767808 1102 4 0.23431170608719043 1102 5 0.2552183876291636 1102 6 0.20558286855052188 1102 7 0.2216570588894058 1102 8 0.24555923270015967 1102 9 0.30603314254630476 1102 10-11 0.3012176694879116 1102 12-13 0.3573785631111832 1102 14-15 0.4048692680121846 1102 16-17 0.45624315084025113 1102 18-19 0.3774783072457808 1102 20-21 0.3726844724215823 1102 22-23 0.507010615672634 1102 24-25 0.5243934961103704 1102 26-27 0.4295625061914663 1102 28-29 0.3735456130452661 1102 30-31 0.3731116943034962 1102 32-33 0.4205682544058007 1102 34-35 0.45436819129454165 1102 36-37 0.3225553069119016 1102 38-39 0.3217921598331728 1102 40-41 0.27163942095902627 1102 42-43 0.2138524872050951 1102 44-45 0.21073177010354982 1102 46-47 0.1390011405653624 1102 48-49 0.2433871497579716 1102 50-51 0.10861588507482622 1102 52-53 0.29531060796274744 1102 54-55 0.30695272376036087 1102 56-57 0.29945359575602737 1102 58-59 0.23012427750660436 1102 60-61 0.22300379464493147 1102 62-63 0.33863832278962036 1102 64-65 0.14401832005283666 1102 66-67 0.09235939903170731 1102 68-69 0.29767692974884596 1102 70-71 0.2874600611439959 1102 72-73 0.2338115840981061 1102 74-75 0.29548390577318173 1102 76-77 0.3305072604329524 1102 78-79 0.36947381797129 1102 80-81 0.32457257899214653 1102 82-83 0.3281603854684292 1102 84-85 0.2394328843599638 1102 86-87 0.27639694468255627 1102 88-89 0.25674058915114983 1102 90-91 0.2819606178963632 1102 92-93 0.286993536349641 1102 94-95 0.22592028482696236 1102 96-97 0.2058935027892943 1102 98-99 0.2856092185917234 1102 100-101 0.36803021544573866 1102 102-103 0.43055678848260825 1102 104-105 0.2962814796808999 1102 106-107 0.11317275915763503 1102 108-109 0.21615319658852528 1102 110-111 0.2645642091765268 1102 112-113 0.2581448915078468 1102 114-115 0.21283029739761616 1102 116-117 0.227154581199148 1102 118-119 0.141017169695008 1102 120-121 0.08027309200575417 1102 122-123 0.06819844428584787 1102 124-125 -0.048558688897138325 1103 1 0.01816174590527453 1103 2 0.13071890339717385 1103 3 0.12123886729583688 1103 4 0.0795035664712529 1103 5 0.17198343397625848 1103 6 0.13668865185586299 1103 7 0.12430086704915766 1103 8 0.10341535251434664 1103 9 0.18613111605827015 1103 10-11 0.18628527975619136 1103 12-13 0.19289831115938227 1103 14-15 0.2612668923913759 1103 16-17 0.20888901135545979 1103 18-19 0.19640631035812817 1103 20-21 0.20743158608696177 1103 22-23 0.23858233529867334 1103 24-25 0.253546179223072 1103 26-27 0.2622834996967285 1103 28-29 0.15473467164143528 1103 30-31 0.19491451173576024 1103 32-33 0.19843210992204519 1103 34-35 0.30389258695976196 1103 36-37 0.27507219173061515 1103 38-39 0.26659915839110226 1103 40-41 0.32735996810925627 1103 42-43 0.2512905163965584 1103 44-45 0.1998261469593885 1103 46-47 0.29647937527118273 1103 48-49 0.16181860547433047 1103 50-51 0.10958839822667699 1103 52-53 0.29792797429761464 1103 54-55 0.2556285414368489 1103 56-57 0.2880221371999241 1103 58-59 0.26967849170229385 1103 60-61 0.27055293371409306 1103 62-63 -0.025532468810339992 1103 64-65 0.11137805898289344 1103 66-67 0.2699627538454976 1103 68-69 0.2869081625716632 1103 70-71 0.24572128236520285 1103 72-73 0.26285028403547983 1103 74-75 0.2597756959899229 1103 76-77 0.268678341944252 1103 78-79 0.3116744219059271 1103 80-81 0.2885424785532251 1103 82-83 0.3390012432445815 1103 84-85 0.3452333572020052 1103 86-87 0.3421318570301928 1103 88-89 0.25018047372358154 1103 90-91 0.15551919315378626 1103 92-93 0.3001147998364573 1103 94-95 0.33185206324073135 1103 96-97 0.1032194191595508 1103 98-99 0.25797083174828117 1103 100-101 0.25094430462071315 1103 102-103 0.13945711190284982 1103 104-105 0.099486084288813 1103 106-107 0.03384341212678521 1103 108-109 0.05828204552948435 1103 110-111 0.17178357602874073 1103 112-113 0.17496841062366641 1103 114-115 0.08357582261731977 1103 116-117 0.04142890057870119 1103 118-119 0.06584209990201373 1103 120-121 -0.06497913860775029 1103 122-123 -0.09344826634339398 1103 124-125 -0.14851553134697326 1104 1 0.14876727314142713 1104 2 0.2635967099988932 1104 3 0.27998235488015766 1104 4 0.2355495408979209 1104 5 0.24373896439409748 1104 6 0.27744060457663267 1104 7 0.24708463785908208 1104 8 0.2617594304712014 1104 9 0.2782751951271649 1104 10-11 0.37032773764828164 1104 12-13 0.3435965829790959 1104 14-15 0.5233496977605796 1104 16-17 0.5317760479337252 1104 18-19 0.41553315087043075 1104 20-21 0.5134095404053767 1104 22-23 0.4949096263129107 1104 24-25 0.335925326148363 1104 26-27 0.5784697833584929 1104 28-29 0.5994738630238174 1104 30-31 0.5935818798147494 1104 32-33 0.6051459602617442 1104 34-35 0.6195778941839194 1104 36-37 0.580006028120323 1104 38-39 0.5046372879555108 1104 40-41 0.537230791683875 1104 42-43 0.4241416895117496 1104 44-45 0.3427996883110964 1104 46-47 0.5216508310435941 1104 48-49 0.5352463741120559 1104 50-51 0.47655496705394285 1104 52-53 0.40848048168190587 1104 54-55 0.46890336676849387 1104 56-57 0.5087051307519133 1104 58-59 0.4855916755561367 1104 60-61 0.588186884212007 1104 62-63 0.5392542130872329 1104 64-65 0.46114910119798935 1104 66-67 0.48093484212459714 1104 68-69 0.5201995573792786 1104 70-71 0.5026096002668439 1104 72-73 0.3707689119691935 1104 74-75 0.4700068840435918 1104 76-77 0.5497462329316818 1104 78-79 0.5616172751301534 1104 80-81 0.5158919060660878 1104 82-83 0.5950556197917933 1104 84-85 0.595011843943027 1104 86-87 0.6197158025548042 1104 88-89 0.601893054478154 1104 90-91 0.5411813142320518 1104 92-93 0.5440381206016127 1104 94-95 0.6004662817292257 1104 96-97 0.5576596309671231 1104 98-99 0.5807567648686351 1104 100-101 0.5724642976518943 1104 102-103 0.5693747155192206 1104 104-105 0.5398613625318305 1104 106-107 0.5690688082775033 1104 108-109 0.6573079246785731 1104 110-111 0.5800311531598972 1104 112-113 0.5851720446643647 1104 114-115 0.6212831804654897 1104 116-117 0.47480132024732313 1104 118-119 0.5349944865676051 1104 120-121 0.5805847579745027 1104 122-123 0.6177261524121178 1104 124-125 0.7140298540045471 1105 1 0.17860354020608682 1105 2 0.22272445633088722 1105 3 0.11610444334410985 1105 4 0.16992966088885453 1105 5 0.17246999558770426 1105 6 0.26500693830423927 1105 7 0.2527190154876635 1105 8 0.20263749742362336 1105 9 0.25832198613484536 1105 10-11 0.3615699653463267 1105 12-13 0.2977472087376398 1105 14-15 0.45672518729251976 1105 16-17 0.4577306426232326 1105 18-19 0.3975561619856762 1105 20-21 0.3779442344427295 1105 22-23 0.38224336934438696 1105 24-25 0.45859416943526554 1105 26-27 0.46060828901221385 1105 28-29 0.37734310446138863 1105 30-31 0.370571357769947 1105 32-33 0.4201684956367657 1105 34-35 0.43254834015773724 1105 36-37 0.35909688975585397 1105 38-39 0.3484190311578246 1105 40-41 0.30398871109751013 1105 42-43 0.3018406254434254 1105 44-45 0.3037001304766349 1105 46-47 0.3144474004939539 1105 48-49 0.27352875025054146 1105 50-51 0.21995635536117675 1105 52-53 0.28005191923595874 1105 54-55 0.2918317910006394 1105 56-57 0.272686613661449 1105 58-59 0.22881593664616418 1105 60-61 0.20859797833146132 1105 62-63 0.18961214531383064 1105 64-65 0.13092797097685605 1105 66-67 0.21200494914835133 1105 68-69 0.30832355117686205 1105 70-71 0.24051458747168653 1105 72-73 0.26461104214642717 1105 74-75 0.22819714928358792 1105 76-77 0.3204497115298963 1105 78-79 0.3493351101681199 1105 80-81 0.2692099864483879 1105 82-83 0.22494361322396372 1105 84-85 0.19349439875102803 1105 86-87 0.24018769888376923 1105 88-89 0.2978967180337264 1105 90-91 0.30549712031512044 1105 92-93 0.31886244670551633 1105 94-95 0.3004802217479927 1105 96-97 0.2786445793060075 1105 98-99 0.342651219351211 1105 100-101 0.3565949209664865 1105 102-103 0.4011902279721049 1105 104-105 0.40925934354255844 1105 106-107 0.34721240114828333 1105 108-109 0.33113171335026337 1105 110-111 0.2937810538001884 1105 112-113 0.2802400067048012 1105 114-115 0.3135939847511864 1105 116-117 0.27436075633501034 1105 118-119 0.2336218989323413 1105 120-121 0.27991017970456156 1105 122-123 0.34668034254448443 1105 124-125 0.3315687703846031 1106 1 -0.08391451285638496 1106 2 0.06267850898628424 1106 3 0.1137659357017391 1106 4 -0.562808146933456 1106 5 0.0043605274466216315 1106 6 0.12132404860168577 1106 7 0.10478458897418363 1106 8 0.18227948922585568 1106 9 0.25321516180657255 1106 10-11 0.25495891762994916 1106 12-13 0.2099470731908113 1106 14-15 0.24709584075382907 1106 16-17 0.26236456873937897 1106 18-19 0.2493272567320446 1106 20-21 0.2555087436917418 1106 22-23 0.2408372766658644 1106 24-25 0.26355938413408353 1106 26-27 0.285477343410669 1106 28-29 0.22223021733552173 1106 30-31 0.19905318994737797 1106 32-33 0.24452654280966613 1106 34-35 0.29464784365792696 1106 36-37 0.29768020883439306 1106 38-39 0.3698217892976743 1106 40-41 0.29574601297936454 1106 42-43 0.21458776564345072 1106 44-45 0.2501282765813926 1106 46-47 0.31487450293141706 1106 48-49 0.21273783665190393 1106 50-51 0.2895291230066306 1106 52-53 0.2702231499622201 1106 54-55 0.3419467676263892 1106 56-57 0.353366209965742 1106 58-59 0.4065381868967961 1106 60-61 0.4109996040273245 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-1.1125063545801765 2216 64-65 -0.9180809487456401 2216 66-67 -0.9336999070493022 2216 68-69 -1.1568683791039192 2216 70-71 -1.0574845645349527 2216 72-73 -1.0463926048950043 2216 74-75 -1.011849528635416 2216 76-77 -1.1382535381701757 2216 78-79 -1.3749671018407454 2216 80-81 -1.4017440874371019 2216 82-83 -1.4889324677641866 2216 84-85 -1.442361866374192 2216 86-87 -1.4427945770021644 2216 88-89 -1.3085848799839255 2216 90-91 -1.4521724819463238 2216 92-93 -1.2843004441095935 2216 94-95 -1.3492342026095159 2216 96-97 -1.4279995348208843 2216 98-99 -1.3450631896926453 2216 100-101 -1.3743234577582584 2216 102-103 -1.385890794156598 2216 104-105 -1.7289064592587842 2216 106-107 -1.5266778258239384 2216 108-109 -1.6957281413901981 2216 110-111 -1.8228353091218281 2216 112-113 -1.9258228586992239 2216 114-115 -1.8969098391150432 2216 116-117 -1.8261118372565157 2216 118-119 -1.9269084606178026 2216 120-121 -1.7630069461722044 2216 122-123 -1.6900316684775163 2216 124-125 -1.5181388585407767 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34418.0 3 7200.0 4 5442.0 5 4709.0 6 5187.0 7 5856.0 8 5973.0 9 6692.0 10 7597.0 11 8343.0 12 8403.0 13 8171.0 14 8011.0 15 7992.0 16 7543.0 17 7514.0 18 7072.0 19 7258.0 20 7500.0 21 8374.0 22 9839.0 23 12645.0 24 17634.0 25 25100.0 26 31065.0 27 30859.0 28 35177.0 29 44107.0 30 50791.0 31 62654.0 32 83437.0 33 114333.0 34 180160.0 35 282380.0 36 567351.0 37 910239.0 38 238644.0 39 497.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.23801902195185 16.164884873019243 11.541983046867136 22.055113058161773 2 16.18744889950961 21.085244429152343 39.060508214987244 23.6667984563508 3 24.826173414503 20.63134108950983 25.399739212144322 29.14274628384284 4 14.33716958279086 15.478378481177415 36.1231489465348 34.06130298949693 5 16.884874570579825 34.52656839983894 30.936430810928115 17.652126218653112 6 36.02661673238161 34.69386117417443 14.477732804822608 14.801789288621354 7 34.949866615680804 29.52562911832372 17.252492364332134 18.272011901663344 8 32.20232953126069 31.660279482344993 16.460129874298538 19.67726111209578 9 33.2599251892859 15.087292540874275 16.79919558071808 34.85358668912175 10-11 30.36187354051596 24.779766845407124 23.29318214884199 21.565177465234928 12-13 28.959268598096344 23.083703775809294 24.773190117672836 23.183837508421526 14-15 25.840626174259906 23.75491379253198 23.14544128098607 27.25901875222204 16-17 26.25529147464192 27.505619874906102 22.917802765854187 23.321285884597792 18-19 26.150953520852067 25.196577868630822 24.122268520989877 24.53020008952723 20-21 27.23890478119384 24.78810550815776 23.54505163167394 24.427938078974464 22-23 26.90249730737951 24.221477673841058 23.47996470547599 25.396060313303447 24-25 24.870358217089233 25.041283358576106 25.914697224551116 24.173661199783545 26-27 24.402027393608762 25.083186707711373 25.211355469702664 25.3034304289772 28-29 24.576697884100042 24.80606576596695 24.372435378517196 26.24480097141581 30-31 24.906730835991063 25.500956503930162 24.223431502770076 25.3688811573087 32-33 26.13692782032589 24.83955052165488 23.732392425144802 25.291129232874425 34-35 26.140329576050526 23.71461607087096 24.737951417345883 25.407102935732635 36-37 25.516046343426602 24.875190454708328 25.12575156995388 24.48301163191119 38-39 24.9836977398735 25.422977795780916 24.685529489384255 24.907794974961334 40-41 24.62189048998192 25.249401029318946 25.279423704201463 24.849284776497672 42-43 24.67694659801749 23.938312038342495 24.79060012902249 26.594141234617524 44-45 24.514935801019273 24.620529787692064 24.611912006523 26.252622404765667 46-47 24.921367108057556 24.481930048039768 24.509527881662162 26.08717496224051 48-49 24.011510843576104 24.44850561743262 24.699973867538073 26.840009671453195 50-51 24.3412411665547 25.009799674931653 24.884405703576537 25.764553454937104 52-53 24.73667793956179 24.383000013607024 25.501148397842833 25.379173648988353 54-55 24.25450087172171 24.609923287791673 25.375719558560267 25.759856281926353 56-57 24.339926710276714 25.237382155002564 24.819139482492076 25.603551652228646 58-59 24.948336925238483 24.2026895153004 25.46285683981429 25.386116719646836 60-61 25.12768795398175 25.163781454465145 25.28113330451436 24.42739728703875 62-63 25.07121880895286 24.669113837400264 24.89982265513489 25.359844698511985 64-65 25.720839623051305 23.661661699730242 25.535815935099706 25.081682742118744 66-67 25.575093674872353 24.85662454357541 24.442080851284302 25.126200930267935 68-69 24.299171890148045 25.25059015497118 25.018907115513013 25.431330839367767 70-71 24.657428121106246 25.147610428862844 24.92107581520094 25.273885634829973 72-73 24.679065593640555 25.147008438462304 24.759783292081885 25.414142675815256 74-75 24.008044925409276 25.340293784932232 25.281534007046986 25.370127282611506 76-77 24.528205116575148 25.36317377057874 24.498880120077228 25.609740992768888 78-79 24.61752552824549 25.833499352264493 23.86705599414404 25.68191912534598 80-81 24.23043475606193 24.962532458640037 24.72671068045172 26.080322104846314 82-83 24.818149957443154 24.254888890788447 25.0416366250879 25.8853245266805 84-85 24.942529036468216 24.236440996622846 25.727048038309903 25.093981928599035 86-87 25.30355868719874 24.406359197302244 24.986296638096917 25.3037854774021 88-89 24.368173001207253 25.057016884954397 25.22698137928255 25.3478287345558 90-91 23.96662857278429 25.66773470259645 25.04458484500802 25.321051879611232 92-93 24.851769440959284 24.186823457897077 25.216703716673095 25.74470338447054 94-95 24.303357308918656 24.372082473687197 25.70356057070326 25.62099964669089 96-97 24.518687847687023 24.221862331509126 25.52238865859256 25.73706116221129 98-99 24.257180902982338 24.32708446328388 25.817249728419345 25.598484905314432 100-101 24.259123866663664 25.321158117830866 25.03366449179832 25.386053523707147 102-103 24.461733119830967 24.989249577789707 25.071763974633015 25.477253327746315 104-105 24.455564636625866 24.268397671795775 25.084490297549944 26.191547394028415 106-107 24.095164809438188 24.86546818050108 24.62664552559912 26.41272148446161 108-109 24.615296317346477 25.070433788401026 24.765776034683253 25.548493859569245 110-111 24.676859373511732 25.452110780704686 24.58893707170587 25.282092774077714 112-113 24.047818959518253 25.832656320243565 24.97470904719181 25.144815673046377 114-115 24.13250113921922 25.431019142372048 25.12979771793281 25.306682000475927 116-117 24.654163557113037 25.222780110161064 24.94287667117792 25.180179661547985 118-119 24.270998351577944 25.56234376914968 24.906728501989036 25.259929377283342 120-121 24.099803741986133 25.60476252780322 25.139779318766625 25.155654411444022 122-123 24.258372913586385 25.828899908845475 25.471382277641847 24.44134489992629 124-125 24.381407599399772 26.65393454148609 24.422891941334285 24.541765917779852 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 2.0 2 2.5 3 6.5 4 15.0 5 17.0 6 19.0 7 27.5 8 33.5 9 51.5 10 74.0 11 85.0 12 106.5 13 146.0 14 210.0 15 267.0 16 337.0 17 452.0 18 623.0 19 773.5 20 958.0 21 1253.0 22 1605.0 23 2062.0 24 2691.0 25 3581.5 26 4652.0 27 5828.0 28 6707.0 29 7915.5 30 9882.5 31 11983.5 32 14764.0 33 17842.5 34 22139.0 35 25978.5 36 29105.5 37 33353.5 38 37142.0 39 40532.5 40 43543.0 41 49006.0 42 55177.5 43 61965.5 44 68844.0 45 76438.5 46 93555.0 47 117325.0 48 139017.5 49 159851.5 50 169105.0 51 157097.0 52 141052.0 53 131746.0 54 125520.0 55 119851.5 56 112501.0 57 105769.0 58 102489.5 59 95277.0 60 85389.5 61 73386.0 62 58480.5 63 46227.0 64 37903.5 65 32269.0 66 26718.0 67 22340.0 68 17921.5 69 12504.5 70 8991.5 71 7198.5 72 5897.0 73 5017.0 74 4078.0 75 3307.0 76 2558.5 77 1860.0 78 1418.5 79 1111.5 80 919.0 81 799.5 82 555.5 83 275.0 84 154.5 85 138.0 86 115.0 87 78.5 88 43.5 89 32.0 90 28.0 91 25.5 92 21.0 93 11.5 94 4.0 95 5.5 96 4.0 97 3.0 98 4.5 99 5.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16157467446942206 2 0.01709600312891747 3 0.008233993343723517 4 0.01514217419989833 5 0.003244751614263928 6 0.0 7 4.5356742995087166E-4 8 3.837878253430453E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.373552552939169E-4 28-29 0.008617781169066563 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 1.7444901151956602E-5 52-53 0.0 54-55 0.0 56-57 2.7911841843130563E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0016572656094358774 66-67 1.2211430806369623E-4 68-69 0.00636738892046416 70-71 0.02095132628349988 72-73 1.7444901151956603E-4 74-75 0.009106238401321347 76-77 4.012327264950019E-4 78-79 9.769144645095699E-4 80-81 6.803511449263075E-4 82-83 1.7444901151956602E-5 84-85 0.020567538458156835 86-87 0.002860963788920883 88-89 0.019834852609774658 90-91 8.722450575978302E-5 92-93 0.015543406926393333 94-95 0.014112925031932892 96-97 0.006053380699728941 98-99 0.002634180073945447 100-101 5.75681738014568E-4 102-103 1.7444901151956602E-5 104-105 3.314531218871755E-4 106-107 8.199103541419603E-4 108-109 0.0 110-111 0.0 112-113 8.199103541419603E-4 114-115 0.0058963765893613315 116-117 0.0 118-119 0.07731580190547166 120-121 0.0014653716967643546 122-123 0.00539047445595459 124-125 0.25319529531949814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2866167.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.1947251175592 #Duplication Level Percentage of deduplicated Percentage of total 1 75.87991781194586 35.05251945265823 2 12.842937786084402 11.865519614601665 3 4.33937784107052 6.013690996484405 4 1.9837272187753467 3.665509343181895 5 1.1137423876949908 2.572451172567207 6 0.7256191582364074 2.011186653285932 7 0.4985203608064964 1.6120307723073775 8 0.34747678019968614 1.284127547684723 9 0.27906174917198623 1.1602062714442642 >10 1.7045872515846976 15.062208233822815 >50 0.1691321112566987 5.409448997608653 >100 0.10705846408311007 9.307332152104399 >500 0.005440664055242723 1.7477905726474317 >1k 0.003022591141783708 2.2238165370713148 >5k 3.778238927229635E-4 1.012161682529704 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 6420 0.22399253079112275 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 6328 0.22078266897916274 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 5700 0.19887187313230528 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 5464 0.19063787978858177 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 5084 0.17737975491309474 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 4512 0.15742278799525639 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2903 0.10128509608826003 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.4889802303913204E-5 0.0 12-13 0.0 0.0 0.0 6.977960460782641E-5 0.0 14-15 0.0 0.0 0.0 1.0466940691173961E-4 0.0 16-17 0.0 0.0 0.0 1.0466940691173961E-4 0.0 18-19 0.0 0.0 0.0 1.0466940691173961E-4 0.0 20-21 0.0 0.0 0.0 3.1400822073521885E-4 0.0 22-23 0.0 0.0 0.0 7.675756506860905E-4 0.0 24-25 0.0 0.0 0.0 0.0013781471910045717 0.0 26-27 0.0 0.0 0.0 0.002459731062425881 0.0 28-29 0.0 0.0 0.0 0.00919346290708113 0.0 30-31 0.0 0.0 0.0 0.03318020199102146 0.0 32-33 0.0 0.0 0.0 0.07431527890733514 0.0 34-35 0.0 0.0 0.0 0.1270686599908519 6.977960460782641E-5 36-37 0.0 0.0 0.0 0.21328136148382143 6.977960460782641E-5 38-39 0.0 0.0 0.0 0.3547595098261895 1.0466940691173961E-4 40-41 0.0 0.0 0.0 0.5274640312305598 1.0466940691173961E-4 42-43 0.0 0.0 0.0 0.7374483063966615 1.0466940691173961E-4 44-45 0.0 0.0 0.0 0.9942198064523107 1.0466940691173961E-4 46-47 0.0 0.0 0.0 1.278292576810772 1.74449011519566E-4 48-49 0.0 0.0 0.0 1.5148454364313035 2.442286161273924E-4 50-51 3.4889802303913204E-5 0.0 0.0 1.7885733804066546 2.442286161273924E-4 52-53 3.4889802303913204E-5 0.0 0.0 2.027987203816107 2.6167351727934905E-4 54-55 3.4889802303913204E-5 0.0 0.0 2.2786529884685716 2.965633195832622E-4 56-57 3.4889802303913204E-5 1.7444901151956602E-5 0.0 2.598348246979328 3.1400822073521885E-4 58-59 3.4889802303913204E-5 3.4889802303913204E-5 0.0 2.9566839615416685 3.1400822073521885E-4 60-61 3.4889802303913204E-5 3.4889802303913204E-5 0.0 3.367860281693286 3.314531218871755E-4 62-63 3.4889802303913204E-5 3.4889802303913204E-5 0.0 3.767400852776548 3.4889802303913206E-4 64-65 3.4889802303913204E-5 3.4889802303913204E-5 0.0 4.147961371406481 3.4889802303913206E-4 66-67 3.4889802303913204E-5 3.4889802303913204E-5 0.0 4.5474844975885915 3.4889802303913206E-4 68-69 3.4889802303913204E-5 3.4889802303913204E-5 0.0 4.975006690119592 3.4889802303913206E-4 70-71 3.4889802303913204E-5 3.4889802303913204E-5 0.0 5.454549577885727 3.4889802303913206E-4 72-73 3.4889802303913204E-5 3.4889802303913204E-5 0.0 5.910663963404785 3.4889802303913206E-4 74-75 3.4889802303913204E-5 3.4889802303913204E-5 0.0 6.359486380242323 3.4889802303913206E-4 76-77 3.4889802303913204E-5 3.4889802303913204E-5 0.0 6.855514699597057 3.4889802303913206E-4 78-79 3.4889802303913204E-5 3.4889802303913204E-5 0.0 7.419490908938663 3.4889802303913206E-4 80-81 3.4889802303913204E-5 3.4889802303913204E-5 0.0 8.29787657174198 3.4889802303913206E-4 82-83 3.4889802303913204E-5 3.4889802303913204E-5 0.0 9.029742509770017 3.4889802303913206E-4 84-85 3.4889802303913204E-5 3.4889802303913204E-5 0.0 9.575715581122804 3.4889802303913206E-4 86-87 3.4889802303913204E-5 3.4889802303913204E-5 0.0 10.109267882855395 3.4889802303913206E-4 88-89 3.4889802303913204E-5 3.4889802303913204E-5 0.0 10.719193961831255 3.4889802303913206E-4 90-91 3.4889802303913204E-5 3.4889802303913204E-5 0.0 11.394643089533862 3.4889802303913206E-4 92-93 3.4889802303913204E-5 3.4889802303913204E-5 0.0 12.114001033435944 3.4889802303913206E-4 94-95 3.4889802303913204E-5 3.4889802303913204E-5 0.0 12.829398984776532 3.4889802303913206E-4 96-97 3.4889802303913204E-5 3.4889802303913204E-5 0.0 13.519676278458304 3.4889802303913206E-4 98-99 3.4889802303913204E-5 3.4889802303913204E-5 0.0 14.234742776677004 3.4889802303913206E-4 100-101 3.4889802303913204E-5 3.4889802303913204E-5 0.0 15.025956268423997 3.4889802303913206E-4 102-103 3.4889802303913204E-5 3.4889802303913204E-5 0.0 15.818704911472361 3.4889802303913206E-4 104-105 3.4889802303913204E-5 3.4889802303913204E-5 0.0 16.59627649051852 3.4889802303913206E-4 106-107 3.4889802303913204E-5 3.4889802303913204E-5 0.0 17.357467307382997 3.4889802303913206E-4 108-109 3.4889802303913204E-5 3.4889802303913204E-5 0.0 18.10855752648049 3.4889802303913206E-4 110-111 3.4889802303913204E-5 3.4889802303913204E-5 0.0 18.946593132919332 3.4889802303913206E-4 112-113 3.4889802303913204E-5 3.4889802303913204E-5 0.0 20.174435055598643 3.4889802303913206E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6205 0.0 57.613503 1 GAGTAGT 2050 0.0 49.700073 1 GTATCAA 10510 0.0 49.5477 1 GATTGTG 6300 0.0 44.093884 9 AGGGTAA 4315 0.0 42.184975 6 GAGTAAG 1835 0.0 41.561256 1 GGGGTAA 6465 0.0 40.8523 9 GATAGCG 1160 0.0 38.972443 9 CGGGATT 1680 0.0 38.949383 6 TATCAAC 13315 0.0 38.87438 2 GGGATTG 7275 0.0 38.185726 7 AGTAGTA 1935 0.0 37.81896 2 TGGGGTA 6380 0.0 37.66715 8 GTGGGGT 6640 0.0 35.47679 7 ATCAACG 14790 0.0 34.834118 3 TCAACGC 14880 0.0 34.503483 4 GAGTAGG 3030 0.0 34.21538 1 GAGTTAG 1760 0.0 34.19192 1 TACGCGG 140 8.083043E-8 33.995155 4 GAGTCAG 6445 0.0 33.83553 1 >>END_MODULE