FastQCFastQC Report
Thu 26 May 2016
SRR935917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866167
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT66400.2316682872979837No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT59440.20738498489446008No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT57790.2016281675143144No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC54310.18948651631255262No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT52940.1847066133969165No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG49150.1714833783237334No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA28960.10104086747213264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69550.067.337421
GTATCAA120700.050.778241
GATTGTG61200.049.67599
AGGGTAA44100.049.106516
CGGGATT16550.047.4517446
GAGTAGT21850.047.0234571
AGTAGTA16600.045.2420772
TAGTAGG29250.044.8308452
GGGATTG72350.042.9247937
GATAGCG10200.041.9960679
GTAGTAG32350.041.5475851
TATCAAC158250.038.4558262
TCGTAGG9250.037.3737262
TAGGGTA53200.036.244835
GAGTAAG17250.035.3222731
ATCAACG174000.034.837283
TGGTATC30150.034.794092
TCAACGC175250.034.5887954
GGGGTAA60300.033.9404349
ACGGGAT7550.033.8948865
Produced by FastQC (version 0.11.5)