FastQCFastQC Report
Thu 26 May 2016
SRR935915_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935915_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519096
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC84070.5534212452669219No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT78120.5142532137534428No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT69720.4589571692638253No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT60080.39549837534955No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG59440.39128534338843624No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA42480.27963999641892284No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT41880.27569027895537873No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC29570.19465524232833212No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC25780.16970619368361184No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC21800.14350640117543592No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG21580.14205817143880306No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA20290.13356627889218325No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG17770.116977465545298No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT16070.1057865993985897No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG16010.10539162765223528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGTA13350.074.874962
GAGTAGT16650.074.439161
GAGTCAG44400.061.0633771
GTGGGGT48150.060.909877
GGTATCA29600.059.9897351
GAGTTAG11100.058.5134771
GAGTCCG5950.057.0834581
GGGGTAA61550.056.2512329
TGGGGTA54450.055.6105238
GAGTAAG10750.054.3212851
GAGTACG7050.054.0932731
AGGGTAA46600.053.4890866
TCGTAGG7900.052.72052
AGTGGGG48850.051.9996456
GTAGTAG27250.051.8243941
CGGGATT15500.051.429256
AGTCAGT39300.050.7178462
GATTGTG48750.050.1538549
AGTCGTA3800.050.1042942
GTAATCG1550.049.902263
Produced by FastQC (version 0.11.5)