FastQCFastQC Report
Thu 26 May 2016
SRR935915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519096
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT83840.5519071869058967No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT74350.4894358223575074No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC71780.47251786588866007No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG66020.4346005782386367No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT60990.40148878016925854No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT45780.30136344246841545No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA36360.23935287829077292No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC25650.16885042156651062No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC24440.16088515801502998No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG23880.1571987550490555No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC20380.13415873651171487No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA20370.13409290788732245No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG20230.13317130714582884No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG17870.117635751789222No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT17240.11348854845250068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGTA13150.070.215862
GAGTAGT17650.068.999521
GGTATCA35500.067.6018141
GTATACG1500.063.677991
GAGTCAG42650.058.0884321
GAGTAAG10600.058.0085451
TCGTAGG7700.057.2493132
AGGGTAA47300.057.1077086
GTAGTAG27200.055.7475051
GATTGTG50700.055.388529
TATCCGG2800.055.315212
GAGTTAG10900.054.768911
GAGTACG5900.053.627121
TAGTAGG28550.052.997682
GATAGCG16350.052.036019
AGTCGTA3900.051.933022
GAGTCGG7150.051.7661931
CGGGATT13950.051.6073076
GTATCCG3150.051.1698151
GAGTCGT6000.050.7433971
Produced by FastQC (version 0.11.5)