##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935914_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1528837 Sequences flagged as poor quality 0 Sequence length 125 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.011731793513633 31.0 31.0 34.0 28.0 34.0 2 31.055606320359857 31.0 31.0 34.0 28.0 34.0 3 31.31537698263451 33.0 31.0 34.0 28.0 34.0 4 34.583221756145356 37.0 35.0 37.0 32.0 37.0 5 34.63491922291258 37.0 35.0 37.0 32.0 37.0 6 34.83157524314234 37.0 35.0 37.0 32.0 37.0 7 34.73619620665905 37.0 35.0 37.0 32.0 37.0 8 34.783111607058174 37.0 35.0 37.0 32.0 37.0 9 36.41007118482873 39.0 37.0 39.0 32.0 39.0 10-11 36.205911421557694 39.0 37.0 39.0 32.0 39.0 12-13 36.04305069801424 39.0 37.0 39.0 31.0 39.0 14-15 37.48127759859292 40.0 38.0 41.0 32.0 41.0 16-17 37.57483237258125 40.0 38.0 41.0 32.0 41.0 18-19 37.57610458145636 40.0 38.0 41.0 32.0 41.0 20-21 37.66891925038445 40.0 38.0 41.0 32.0 41.0 22-23 37.57322657680315 40.0 38.0 41.0 31.5 41.0 24-25 37.50974139165915 40.0 38.0 41.0 31.0 41.0 26-27 37.47529102186826 40.0 38.0 41.0 31.0 41.0 28-29 37.3589032055085 40.0 37.0 41.0 31.0 41.0 30-31 37.281677183375336 40.0 37.0 41.0 31.0 41.0 32-33 37.18824308935485 40.0 37.0 41.0 30.5 41.0 34-35 36.973099486734036 40.0 37.0 41.0 30.0 41.0 36-37 36.956645476267255 40.0 36.5 41.0 30.0 41.0 38-39 37.00814148270875 40.0 36.5 41.0 30.0 41.0 40-41 36.93035588489813 40.0 36.0 41.0 30.0 41.0 42-43 36.772229151963224 40.0 35.5 41.0 30.0 41.0 44-45 36.56643841037338 40.0 35.0 41.0 30.0 41.0 46-47 36.38905978858439 40.0 35.0 41.0 30.0 41.0 48-49 36.19776764952706 39.0 35.0 41.0 30.0 41.0 50-51 35.93872368342734 39.0 35.0 41.0 29.5 41.0 52-53 35.694312081667306 39.0 35.0 41.0 29.0 41.0 54-55 35.43569818103565 38.0 35.0 40.0 29.0 41.0 56-57 35.127276158282406 37.5 35.0 40.0 28.0 41.0 58-59 34.74663616853857 37.0 34.0 40.0 28.0 41.0 60-61 34.44935627539103 36.5 34.0 39.0 28.0 41.0 62-63 34.05347921328435 36.0 34.0 39.0 26.5 41.0 64-65 33.67370393312041 35.0 34.0 38.5 26.0 40.5 66-67 33.39840283823586 35.0 33.5 37.0 26.5 40.0 68-69 33.20697693737135 35.0 33.5 37.0 26.5 39.5 70-71 33.0001040006227 35.0 34.0 36.5 27.0 39.0 72-73 32.755490611490956 35.0 34.0 36.0 26.5 39.0 74-75 32.47057011309904 35.0 33.0 35.5 26.0 37.0 76-77 32.27917070295918 35.0 33.0 35.0 26.0 37.0 78-79 32.07672237131885 35.0 33.0 35.0 26.0 36.5 80-81 31.887718900052782 35.0 33.0 35.0 25.0 36.0 82-83 31.780790888760542 35.0 33.0 35.0 25.5 36.0 84-85 31.592713611719233 35.0 33.0 35.0 25.0 35.5 86-87 31.451650175918033 35.0 33.0 35.0 25.0 35.0 88-89 31.34158906410559 35.0 33.0 35.0 24.0 35.0 90-91 31.259758561573275 35.0 33.0 35.0 24.0 35.0 92-93 31.15252214591876 35.0 33.0 35.0 23.0 35.0 94-95 31.01237084136504 35.0 32.5 35.0 23.0 35.0 96-97 30.900396183504192 35.0 32.0 35.0 21.5 35.0 98-99 30.74865207997975 35.0 32.0 35.0 20.0 35.0 100-101 30.636830152593113 35.0 32.0 35.0 19.0 35.0 102-103 29.921360485126932 34.0 30.5 35.0 18.0 35.0 104-105 30.492123097491756 35.0 32.0 35.0 19.0 35.0 106-107 30.54978686413267 35.0 32.0 35.0 18.5 35.0 108-109 30.454048404113713 35.0 32.0 35.0 17.5 35.0 110-111 30.361840405484692 35.0 32.0 35.0 17.0 35.0 112-113 30.280374232177792 35.0 32.0 35.0 12.5 35.0 114-115 30.104315895023475 35.0 31.0 35.0 6.0 35.0 116-117 29.901571913814223 35.0 31.0 35.0 2.0 35.0 118-119 29.676811524053903 35.0 31.0 35.0 2.0 35.0 120-121 29.471142770615835 35.0 31.0 35.0 2.0 35.0 122-123 29.093509314596652 35.0 31.0 35.0 2.0 35.0 124-125 26.253446901141196 32.0 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0025992034789439344 1101 2 0.21132882510675444 1101 3 0.14566786516282804 1101 4 0.2510098309808271 1101 5 0.059625861477783815 1101 6 0.18173266462208204 1101 7 0.052063354914473337 1101 8 0.10232170942226304 1101 9 0.1873985379164509 1101 10-11 0.26686571379381263 1101 12-13 0.12932212180394487 1101 14-15 0.26778715800673325 1101 16-17 0.2332865147059664 1101 18-19 0.33078854968143645 1101 20-21 0.23513940972869563 1101 22-23 0.313631910872914 1101 24-25 0.29966717827411316 1101 26-27 0.31696232965727233 1101 28-29 0.22550569604543114 1101 30-31 0.10812010839810426 1101 32-33 0.14782494413710623 1101 34-35 -0.04856785616988901 1101 36-37 -0.03817111374352322 1101 38-39 0.06235807144471295 1101 40-41 0.06103491372326886 1101 42-43 -0.017166856066232583 1101 44-45 0.0013605226856867603 1101 46-47 -0.09875176321229162 1101 48-49 -0.08059756184909617 1101 50-51 -0.07465623671993171 1101 52-53 -0.042584464879269035 1101 54-55 -0.06591734483713907 1101 56-57 -0.22857990167068465 1101 58-59 -0.22245850254551414 1101 60-61 -0.10944969975228247 1101 62-63 0.03746894005650603 1101 64-65 0.007743313665706353 1101 66-67 -0.19503479935234225 1101 68-69 -0.10792119266699984 1101 70-71 -0.24373066764924545 1101 72-73 -0.1548284673606446 1101 74-75 -0.10356134272981166 1101 76-77 0.0250439924972099 1101 78-79 -0.01407475317441964 1101 80-81 -0.03538331373964354 1101 82-83 -0.024292147786521667 1101 84-85 0.004104146999079461 1101 86-87 -0.07844743587514813 1101 88-89 -0.08323169827950494 1101 90-91 -0.16790142120781582 1101 92-93 -0.4000597696360799 1101 94-95 -0.2955936776285668 1101 96-97 -0.19474168668924818 1101 98-99 -0.28997281977530776 1101 100-101 -0.2811701898071952 1101 102-103 -0.25045872674777314 1101 104-105 -0.26885037684152024 1101 106-107 -0.25083717753921775 1101 108-109 -0.048267593645736184 1101 110-111 -0.2104566194392703 1101 112-113 -0.1944667258711732 1101 114-115 -0.23887467672738438 1101 116-117 -0.2652519220230971 1101 118-119 -0.10445339328301984 1101 120-121 -0.4329370955818135 1101 122-123 -0.3579907167175982 1101 124-125 -0.13957900212160368 1102 1 0.061337960691702165 1102 2 0.06994112766375693 1102 3 0.1379586880864352 1102 4 0.0834061467073397 1102 5 0.14561388815857867 1102 6 0.05675559164863131 1102 7 0.09791968647630256 1102 8 0.07586192712933126 1102 9 0.05159079456244342 1102 10-11 0.14453735320437744 1102 12-13 0.13189108800865768 1102 14-15 0.19304220337661349 1102 16-17 0.24213947983979267 1102 18-19 0.31784118785592597 1102 20-21 0.14911264918938372 1102 22-23 0.22962158943884958 1102 24-25 0.2574763228824182 1102 26-27 0.24497260552209355 1102 28-29 0.2823413676506661 1102 30-31 0.13915219484296415 1102 32-33 0.1946125971500834 1102 34-35 0.22191523479202147 1102 36-37 0.14138139626746948 1102 38-39 0.07989368665801067 1102 40-41 0.1721418476535561 1102 42-43 0.0975320736261196 1102 44-45 0.07973791578308465 1102 46-47 -0.01535605697443998 1102 48-49 0.12041977155258365 1102 50-51 0.03339186161849739 1102 52-53 0.17361027529058504 1102 54-55 0.15216168362655225 1102 56-57 0.055724911011232336 1102 58-59 -0.058217828576374586 1102 60-61 -0.08162796663996374 1102 62-63 -0.04518911402279002 1102 64-65 -0.1106131175788434 1102 66-67 -0.19058097523824102 1102 68-69 0.014452167552192918 1102 70-71 -0.1100732230585919 1102 72-73 -0.16123037671202667 1102 74-75 -0.0395707299943453 1102 76-77 -0.021262904977689345 1102 78-79 -0.01301811629864602 1102 80-81 0.06485307672043561 1102 82-83 0.016431947521557078 1102 84-85 -0.004503883441174139 1102 86-87 0.07594173687216355 1102 88-89 0.09068275685472571 1102 90-91 0.057507989980571494 1102 92-93 0.1213241683441737 1102 94-95 0.03700769970187068 1102 96-97 -0.030270318415695385 1102 98-99 0.07387880676194314 1102 100-101 0.18215852964004853 1102 102-103 0.11747020801415431 1102 104-105 0.05333216216290637 1102 106-107 0.04348546410992782 1102 108-109 0.08525200397783195 1102 110-111 0.0889122465130967 1102 112-113 0.10813242995855887 1102 114-115 0.06498219027307783 1102 116-117 0.060514068509895935 1102 118-119 0.03358040770469373 1102 120-121 -0.13134900738253918 1102 122-123 -0.19610426388461732 1102 124-125 -0.3302714837657419 1103 1 0.09730693172014071 1103 2 0.15192794432439172 1103 3 0.18148887378344014 1103 4 0.213231354876946 1103 5 0.33709615290263883 1103 6 0.1834719423448874 1103 7 0.25381399900886237 1103 8 0.2401991398199499 1103 9 0.2130573417284225 1103 10-11 0.25439869855842545 1103 12-13 0.2117875992361249 1103 14-15 0.2501836571642784 1103 16-17 0.3119012437405786 1103 18-19 0.3149188662460247 1103 20-21 0.17236561389547234 1103 22-23 0.28446379555104073 1103 24-25 0.33654247357690537 1103 26-27 0.3619304815298321 1103 28-29 0.25536905442935875 1103 30-31 0.36421003199825464 1103 32-33 0.2732941969876137 1103 34-35 0.33320683560086195 1103 36-37 0.3734852117537031 1103 38-39 0.276197186026657 1103 40-41 0.3447701931577143 1103 42-43 0.22696798475999458 1103 44-45 0.2280904398671879 1103 46-47 0.21702921795072427 1103 48-49 0.028335514737953815 1103 50-51 0.025565964701087296 1103 52-53 0.16957922424377614 1103 54-55 0.13973497345691754 1103 56-57 -0.008054128980290898 1103 58-59 0.05778507211149275 1103 60-61 0.10512600028247476 1103 62-63 -0.09900128896473603 1103 64-65 -0.04927107482350124 1103 66-67 0.10593279088772789 1103 68-69 0.18535080683377458 1103 70-71 0.2268752268173415 1103 72-73 0.25589659571156886 1103 74-75 0.2986439562280552 1103 76-77 0.21601998493746066 1103 78-79 0.2648407389671874 1103 80-81 0.1370339569048049 1103 82-83 0.274186541381102 1103 84-85 0.36395466806921206 1103 86-87 0.29949812589105207 1103 88-89 0.22688738117812335 1103 90-91 0.100491150979785 1103 92-93 0.24102721207965772 1103 94-95 0.2647440373295744 1103 96-97 0.2086672218716359 1103 98-99 0.37423057587248465 1103 100-101 0.38157530153207375 1103 102-103 0.4126768985809548 1103 104-105 0.40861278274233825 1103 106-107 0.3571505958108716 1103 108-109 0.26549719378083125 1103 110-111 0.38868343654567994 1103 112-113 0.24173383041242502 1103 114-115 0.13462563083325918 1103 116-117 0.18328215034726014 1103 118-119 0.24019338000466917 1103 120-121 0.2928058416669046 1103 122-123 0.24686885287579585 1103 124-125 0.05811351301127132 1104 1 0.09180038366824661 1104 2 0.23146196904863814 1104 3 0.3369278005417655 1104 4 0.3243418052551519 1104 5 0.3932294721906757 1104 6 0.4073290065613264 1104 7 0.45303091724486677 1104 8 0.2923839192522024 1104 9 0.47206133784104765 1104 10-11 0.5109556703477693 1104 12-13 0.5299160525094706 1104 14-15 0.6059995131683067 1104 16-17 0.5933060676193946 1104 18-19 0.45624142321441497 1104 20-21 0.48050293609225747 1104 22-23 0.5019843367932282 1104 24-25 0.4219224270915234 1104 26-27 0.4761956011753483 1104 28-29 0.5492791452933474 1104 30-31 0.5088907167742889 1104 32-33 0.5737336504444528 1104 34-35 0.669457540697266 1104 36-37 0.534273526522334 1104 38-39 0.5044623770313237 1104 40-41 0.48694098959383325 1104 42-43 0.5019704483498373 1104 44-45 0.30263781894739594 1104 46-47 0.4521380916314328 1104 48-49 0.5221349139618425 1104 50-51 0.526610866599242 1104 52-53 0.4082556308686236 1104 54-55 0.4615804740923579 1104 56-57 0.35373380860423254 1104 58-59 0.3703623778616745 1104 60-61 0.472489721288575 1104 62-63 0.3419592052483793 1104 64-65 0.33875451932640743 1104 66-67 0.38826400949820083 1104 68-69 0.51076893749579 1104 70-71 0.4452418837429164 1104 72-73 0.4410689280685318 1104 74-75 0.518425053527956 1104 76-77 0.5635373659885445 1104 78-79 0.6287176308342808 1104 80-81 0.5237746615254828 1104 82-83 0.5334453916152277 1104 84-85 0.5379626908769595 1104 86-87 0.5329874016296188 1104 88-89 0.5546285071576094 1104 90-91 0.6383403860776156 1104 92-93 0.6400421159486847 1104 94-95 0.7155915971863109 1104 96-97 0.6623963323288109 1104 98-99 0.689048898864403 1104 100-101 0.6547899634595851 1104 102-103 0.7254784759580666 1104 104-105 0.6282018899373796 1104 106-107 0.7017579675512877 1104 108-109 0.710657259159408 1104 110-111 0.6557947670201187 1104 112-113 0.593491134531547 1104 114-115 0.6939344324751993 1104 116-117 0.5758233347732968 1104 118-119 0.6544999559206168 1104 120-121 0.6359002294393434 1104 122-123 0.6432184073907905 1104 124-125 0.7164998276847498 1105 1 0.048138857931324 1105 2 0.007472951098598912 1105 3 0.12727518254052228 1105 4 0.22580285114278809 1105 5 0.14083514898494087 1105 6 0.27170183829571215 1105 7 0.11731119255100708 1105 8 0.2605175943660143 1105 9 0.2641901028691578 1105 10-11 0.3014556719888404 1105 12-13 0.3260523139518412 1105 14-15 0.3949560871363147 1105 16-17 0.3998007718819139 1105 18-19 0.3749907462724522 1105 20-21 0.31601137284043546 1105 22-23 0.3064199066881059 1105 24-25 0.3426972425530366 1105 26-27 0.3029702558118643 1105 28-29 0.2352428973077636 1105 30-31 0.34444469325445937 1105 32-33 0.3391913124428143 1105 34-35 0.3909623867811689 1105 36-37 0.3951434913049354 1105 38-39 0.3620210699507638 1105 40-41 0.42600021257933207 1105 42-43 0.40283086209676355 1105 44-45 0.3257157960971142 1105 46-47 0.2558007168862133 1105 48-49 0.23653063864257007 1105 50-51 0.1614097040642406 1105 52-53 0.3019478446411554 1105 54-55 0.37316611012241196 1105 56-57 0.3634423871409922 1105 58-59 0.2636480161892294 1105 60-61 0.25631015197136264 1105 62-63 0.33200856867676976 1105 64-65 0.1861418515678821 1105 66-67 0.28858656208632993 1105 68-69 0.25176725047436577 1105 70-71 0.13067228754495375 1105 72-73 0.23851599530978973 1105 74-75 0.2147989421123313 1105 76-77 0.2576613456523873 1105 78-79 0.33214525466132727 1105 80-81 0.2812382543559657 1105 82-83 0.2826100862610694 1105 84-85 0.31148416757866926 1105 86-87 0.29691066184774684 1105 88-89 0.3349989954419996 1105 90-91 0.37654682629830916 1105 92-93 0.4041454837554568 1105 94-95 0.35396879088853694 1105 96-97 0.2953132711398254 1105 98-99 0.33341805459879836 1105 100-101 0.33440194853240257 1105 102-103 0.4738821237316273 1105 104-105 0.38269309215348457 1105 106-107 0.3451576603427178 1105 108-109 0.3241045266113822 1105 110-111 0.24609677339403646 1105 112-113 0.2366348201810311 1105 114-115 0.264591993383565 1105 116-117 0.1884049370497891 1105 118-119 0.03433127018907456 1105 120-121 0.11903863083884758 1105 122-123 0.17889776172765082 1105 124-125 0.35493856156532644 1106 1 -0.0440939682994248 1106 2 0.05220349379285594 1106 3 0.12462785506564344 1106 4 -0.6511200440665093 1106 5 -0.049783453175756165 1106 6 0.05377292714890558 1106 7 0.1867755851302988 1106 8 0.10339453537202559 1106 9 0.27433530429282627 1106 10-11 0.24816541087825073 1106 12-13 0.31703037625301533 1106 14-15 0.25329296388833455 1106 16-17 0.3549733757434481 1106 18-19 0.24110503333661626 1106 20-21 0.24463865243979654 1106 22-23 0.37461575537625436 1106 24-25 0.4086410518068746 1106 26-27 0.4053947416224446 1106 28-29 0.27434609740855365 1106 30-31 0.3185454523334812 1106 32-33 0.43516402024463474 1106 34-35 0.4952160640623475 1106 36-37 0.4253007403521494 1106 38-39 0.45074473053849573 1106 40-41 0.44582918357054524 1106 42-43 0.3022639151063089 1106 44-45 0.2810636023272366 1106 46-47 0.32031298498914396 1106 48-49 0.30213467341531697 1106 50-51 0.24038856954032894 1106 52-53 0.29458737659491874 1106 54-55 0.31694426061533676 1106 56-57 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-0.32651456593176675 2216 26-27 -0.31451047489814243 2216 28-29 -0.37731312786338833 2216 30-31 -0.21318438045803134 2216 32-33 -0.16407246522235397 2216 34-35 -0.34613340029440565 2216 36-37 -0.37242346166971885 2216 38-39 -0.4259316408042437 2216 40-41 -0.445937616149628 2216 42-43 -0.38970966622779457 2216 44-45 -0.40221400627597603 2216 46-47 -0.5157829232725177 2216 48-49 -0.43345285384693 2216 50-51 -0.4467299303842367 2216 52-53 -0.31531636536357865 2216 54-55 -0.4435726248351912 2216 56-57 -0.27670634187896326 2216 58-59 -0.4679493030247528 2216 60-61 -0.3965954014864437 2216 62-63 -0.407091877278674 2216 64-65 -0.2297831936663428 2216 66-67 -0.316437911420941 2216 68-69 -0.511373661341004 2216 70-71 -0.392801434491588 2216 72-73 -0.4928788401976476 2216 74-75 -0.3287917291790521 2216 76-77 -0.5648046602459615 2216 78-79 -0.7805611418586196 2216 80-81 -0.7068480184418711 2216 82-83 -0.9235385051962943 2216 84-85 -0.8221803453444956 2216 86-87 -0.7671222661438506 2216 88-89 -0.678207539342381 2216 90-91 -0.7357031923007185 2216 92-93 -0.6507490071436521 2216 94-95 -0.6220000427632009 2216 96-97 -0.6401700508001475 2216 98-99 -0.5344444824764203 2216 100-101 -0.6580038596969295 2216 102-103 -0.6684911186606719 2216 104-105 -0.9288019464693207 2216 106-107 -0.7074682761005597 2216 108-109 -1.0229100845005874 2216 110-111 -1.2037013043793579 2216 112-113 -1.2120510009061896 2216 114-115 -1.0751012701203777 2216 116-117 -1.0427481111546655 2216 118-119 -1.1719001395607442 2216 120-121 -0.953085574140637 2216 122-123 -0.7629325646466079 2216 124-125 -0.6935045247215079 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22786.0 3 4668.0 4 3264.0 5 2770.0 6 3066.0 7 3535.0 8 4152.0 9 4540.0 10 4910.0 11 5072.0 12 5075.0 13 4754.0 14 4678.0 15 4707.0 16 4472.0 17 4350.0 18 4250.0 19 4355.0 20 4449.0 21 5028.0 22 5693.0 23 7157.0 24 10103.0 25 14279.0 26 17393.0 27 17153.0 28 19867.0 29 24826.0 30 29140.0 31 36532.0 32 49644.0 33 67525.0 34 108254.0 35 165514.0 36 342302.0 37 440907.0 38 67579.0 39 88.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 61.6720397105173 12.55130377929925 8.528039296483927 17.24861721369953 2 13.760095878958047 27.62034915746104 40.39561845722278 18.22393650635813 3 33.03022532605752 19.684729296050676 21.13987013955484 26.14517523833696 4 14.68903305828917 15.248105623238997 42.34958324692546 27.71327807154637 5 17.186404357973394 33.40550398224995 28.93561157102638 20.472480088750274 6 33.850175002305676 36.48976313367612 14.274641443136188 15.385420420882017 7 40.669202366507825 25.44708879637109 16.57353475031642 17.310174086804658 8 38.5264296707746 26.448116240447767 15.717891644007743 19.307562444769893 9 46.40246147888886 12.33336189534921 12.966980783432112 28.297195842329824 10-11 36.423176571472304 24.842609120527566 20.03758412440306 18.696630183597073 12-13 29.062025578920448 26.4816327705308 23.444749178624015 21.011592471924736 14-15 25.57022102421645 27.015339110709647 21.56793039414928 25.846509470924634 16-17 25.08822065400039 30.75674515988297 21.46755998186857 22.687474204248065 18-19 24.641802886769483 25.621240197614263 24.541824929668763 25.195131985947487 20-21 25.394008648403982 26.40601973918737 22.610880034954675 25.58909157745397 22-23 26.63276071942267 23.723719402395417 22.381718914442807 27.261800963739102 24-25 23.82824329866428 24.16026038093008 28.824426672038943 23.187069648366702 26-27 21.88991757030595 23.477647437432054 28.19161600661426 26.440818985647734 28-29 23.848644886422175 23.93384747069342 23.746696151230825 28.47081149165358 30-31 23.839853431072118 26.33456019183209 22.627657493898955 27.197928883196838 32-33 28.121277807902345 24.691121421054042 21.028337226270686 26.15926354477292 34-35 27.399977891691528 21.209030132054625 24.90729881602813 26.483693160225712 36-37 25.52950379929319 25.14087505731481 26.322917354825925 23.006703788566078 38-39 23.762932215795406 27.38051865568403 24.495351695439084 24.361197433081486 40-41 22.58762706554067 27.125324674899943 25.54376300416591 24.74328525539348 42-43 23.295681619427057 22.895573563434166 23.744519526934525 30.064225290204256 44-45 24.60455234926941 23.982805230381 22.878174717121578 28.53446770322801 46-47 26.086626631877696 22.686166020314786 23.66593037714289 27.56127697066463 48-49 21.491761384634202 23.213494963818903 25.434333418147258 29.860410233399637 50-51 24.097532961329428 24.507092646240245 24.93264487973538 26.462729512694942 52-53 25.376577097493065 22.33037269506167 26.588217056494578 25.70483315095069 54-55 23.469702787151277 23.59447082978761 26.26588053533503 26.669945847726083 56-57 22.822037615002046 25.12072128288827 24.97992748056277 27.07731362154691 58-59 23.736964764719847 23.329171128118954 28.069571837939556 24.86429226922164 60-61 25.15905881398736 26.524410385148972 26.192066256899853 22.12446454396381 62-63 26.0918593676108 24.818603945351924 24.657174047985496 24.432362639051778 64-65 28.730310627829414 21.14498429167318 26.52457111983396 23.600133960663445 66-67 28.415221912364018 23.93449131708415 24.020537199718348 23.62974957083349 68-69 24.307538635790525 26.630837396887554 24.62191692916644 24.43970703815549 70-71 24.969447730103735 26.35630488940994 23.470521966231566 25.203725414254762 72-73 24.055302221854475 26.393290841297183 23.5359199861594 26.01548695068894 74-75 23.057238008827184 26.745664743665643 24.783359837559946 25.413737409947228 76-77 24.054505733142335 26.063460293165363 23.290653637095033 26.591380336597265 78-79 24.131110353809053 27.066120973117158 21.80853325344596 26.99423541962783 80-81 23.189073166392326 23.375131269240693 25.062834264386964 28.372961299980016 82-83 24.557065272491442 21.153072564308687 26.787813220114376 27.502048943085494 84-85 25.438121887088954 21.972727742583807 28.61611252243193 23.973037847895306 86-87 26.69602322621917 23.192107219470046 24.597565590292188 25.51430396401859 88-89 23.63845536611798 24.622505079224783 25.95012006629897 25.788919488358264 90-91 22.201316688851314 26.040383690849566 25.576631879830064 26.18166774046905 92-93 24.457006337133958 23.239657754206604 25.829855770834794 26.47348013782465 94-95 23.7863218648345 22.954944287529663 26.490286093716016 26.768447753919826 96-97 23.56856765513357 21.755953140218914 26.885011162853374 27.790468041794142 98-99 23.1020788083605 23.41536691404184 27.405824692754276 26.076729584843385 100-101 22.673702139683837 26.060161274286642 25.189884512540356 26.076252073489165 102-103 22.915686891408306 25.324413263153627 25.514754025445484 26.245145819992583 104-105 23.104651698996978 23.56235509738339 25.394836634717365 27.93815656890227 106-107 22.53488559956594 25.043620285861273 23.814795543094224 28.60669857147856 108-109 24.271619538250317 25.072031877826085 24.821285722415144 25.835062861508458 110-111 25.04433108303894 25.565151811474994 23.55287058070939 25.837646524776677 112-113 23.32136767007736 25.96347245815234 24.725130967105983 25.99002890466432 114-115 23.91636889917334 24.007653374107722 25.406062191841304 26.669915534877635 116-117 25.368335538713417 24.140048939160945 24.4092404880311 26.082375034094547 118-119 23.631433387336966 25.08053087207685 25.01631564305235 26.271720097533834 120-121 22.42183690349302 25.020399900183577 25.722516224257287 26.835246972066116 122-123 23.333068410058058 25.605227034340928 26.74580350084661 24.315901054754406 124-125 24.267652244729884 26.64854061575242 23.60728521678805 25.47652192272965 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12.0 1 13.5 2 18.5 3 25.0 4 32.5 5 38.5 6 43.0 7 46.5 8 49.0 9 57.5 10 66.5 11 87.0 12 112.0 13 133.0 14 153.0 15 184.5 16 245.0 17 310.5 18 381.0 19 470.0 20 579.5 21 687.0 22 845.5 23 1072.0 24 1344.0 25 1704.5 26 2088.5 27 2441.0 28 2829.0 29 3375.0 30 4182.5 31 4919.0 32 5714.5 33 6880.0 34 8167.5 35 9111.5 36 10554.0 37 12418.5 38 13842.5 39 14915.0 40 15509.5 41 16914.0 42 18410.5 43 20574.0 44 23722.0 45 28937.0 46 45563.0 47 73866.5 48 96341.0 49 113891.0 50 121675.0 51 111732.5 52 95393.5 53 80305.0 54 70632.0 55 64332.0 56 58622.5 57 53502.0 58 50616.0 59 46353.5 60 42522.0 61 36970.0 62 27769.5 63 20263.5 64 15680.5 65 13773.5 66 12080.5 67 10019.0 68 7919.0 69 5499.0 70 3795.5 71 2980.5 72 2546.0 73 2279.0 74 1794.0 75 1431.5 76 1111.0 77 769.0 78 605.5 79 540.5 80 466.5 81 359.5 82 249.0 83 128.5 84 55.5 85 49.0 86 40.5 87 20.0 88 12.5 89 13.0 90 12.5 91 11.0 92 8.5 93 6.5 94 4.0 95 2.5 96 2.0 97 2.5 98 2.0 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15632798002664772 2 0.015894434789320247 3 0.007783694403000451 4 0.015305752019345423 5 0.0034666874231850746 6 0.0 7 1.3081839332773867E-4 8 3.92455179983216E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0012427747366135175 28-29 0.00833967257464334 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.5975058165128133E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.00196227589991608 66-67 9.8113794995804E-5 68-69 0.006900670248038214 70-71 0.020211441769135623 72-73 9.8113794995804E-5 74-75 0.0098113794995804 76-77 4.578643766470853E-4 78-79 0.001177365539949648 80-81 8.83024154962236E-4 82-83 0.0 84-85 0.021192579719093666 86-87 0.0031069368415337934 88-89 0.020047918777475953 90-91 1.3081839332773867E-4 92-93 0.014913296839362208 94-95 0.015011410634358012 96-97 0.006933374846370149 98-99 0.00343398282485314 100-101 8.503195566303013E-4 102-103 0.0 104-105 5.559781716428893E-4 106-107 0.0012100701382815825 108-109 0.0 110-111 0.0 112-113 0.001177365539949648 114-115 0.006508215068054999 116-117 0.0 118-119 0.07600548652341617 120-121 0.001994980498248015 122-123 0.006050350691407913 124-125 0.24698512660277058 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1528837.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.76516687968937 #Duplication Level Percentage of deduplicated Percentage of total 1 78.43327384052103 33.54212044710256 2 11.357784757062067 9.71435121038703 3 3.699176256184039 4.745876697392848 4 1.7239501298274456 2.9490005997733144 5 0.9877921176039207 2.112154737588671 6 0.6616854656114237 1.6978253615242498 7 0.46246249266465655 1.3844099972080812 8 0.32883741099104213 1.1250229405853531 9 0.2743669527645052 1.0560013669121322 >10 1.7527873752251444 14.602650531589623 >50 0.1808300743389235 5.381723237168074 >100 0.11992150799740532 10.092076598762807 >500 0.01009541846217838 2.9805224967356083 >1k 0.005965474545832679 4.611358383973679 >5k 7.648044289529076E-4 2.566909913405364 >10k+ 3.05921771581163E-4 1.4379954798906784 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 11242 0.7353301888952191 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 10741 0.7025601813666205 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 9693 0.6340113432628854 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 8913 0.5829921698650674 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 8502 0.556108990036217 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 6375 0.416983628732167 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 5758 0.37662615439055963 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3754 0.24554612427616548 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 3377 0.22088685713388673 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3053 0.1996942774147931 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 2901 0.18975207952188494 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2755 0.18020233680896 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 2725 0.17824006090904393 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2712 0.17738974135241364 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2629 0.17196077802931248 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2140 0.13997568086068038 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 2101 0.13742472219078947 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 2060 0.13474294512757082 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 2035 0.1331077152109741 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1957 0.1280057978711923 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 1890 0.12362338169471304 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1874 0.12257683454809114 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 1860 0.12166110579479696 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1612 0.10543962502215737 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1597 0.10445848707219933 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1563 0.10223457438562776 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC 1531 0.10014148009238395 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.270459833193467E-5 0.0 20-21 0.0 0.0 0.0 3.2704598331934667E-4 0.0 22-23 0.0 0.0 0.0 0.001667934514928668 0.0 24-25 0.0 0.0 0.0 0.0028125954565463816 0.0 26-27 0.0 0.0 0.0 0.004447825373143114 0.0 28-29 0.0 0.0 0.0 0.012133405981147762 0.0 30-31 0.0 0.0 0.0 0.036629150131766826 0.0 32-33 0.0 0.0 0.0 0.08231747400147955 0.0 34-35 0.0 0.0 0.0 0.13889642911572653 0.0 36-37 0.0 0.0 0.0 0.22036358356057578 0.0 38-39 0.0 0.0 0.0 0.35827887472634423 0.0 40-41 0.0 0.0 0.0 0.5434196058834264 0.0 42-43 0.0 0.0 0.0 0.8894996654319591 0.0 44-45 0.0 0.0 0.0 1.3736912437362518 0.0 46-47 0.0 0.0 0.0 1.8803181764962518 0.0 48-49 0.0 0.0 0.0 2.115889398281177 0.0 50-51 0.0 0.0 0.0 2.4107540568419 0.0 52-53 0.0 0.0 0.0 2.648451077518401 0.0 54-55 0.0 0.0 0.0 2.8941607247862264 0.0 56-57 0.0 0.0 0.0 3.241254626883049 0.0 58-59 0.0 0.0 0.0 3.633775216062929 0.0 60-61 0.0 0.0 0.0 4.146060044334353 0.0 62-63 0.0 0.0 0.0 4.754332868710007 0.0 64-65 0.0 0.0 0.0 5.353579223946046 0.0 66-67 0.0 0.0 0.0 5.8675319867323985 0.0 68-69 0.0 0.0 0.0 6.305675490585328 0.0 70-71 0.0 0.0 0.0 6.820151526944992 0.0 72-73 0.0 0.0 0.0 7.328184757433265 0.0 74-75 0.0 0.0 0.0 7.8882182992693135 0.0 76-77 0.0 0.0 0.0 8.565039961748703 0.0 78-79 0.0 0.0 0.0 9.374511475062416 0.0 80-81 0.0 0.0 0.0 10.755397730431694 0.0 82-83 0.0 0.0 0.0 11.79867441722041 0.0 84-85 0.0 0.0 0.0 12.427747366135172 0.0 86-87 0.0 0.0 0.0 13.000535701320677 3.270459833193467E-5 88-89 0.0 0.0 0.0 13.623067730569053 6.540919666386934E-5 90-91 0.0 0.0 0.0 14.335079540853602 6.540919666386934E-5 92-93 0.0 0.0 0.0 15.199527483963301 6.540919666386934E-5 94-95 0.0 0.0 0.0 16.291174271684948 6.540919666386934E-5 96-97 0.0 0.0 0.0 17.543564160208057 6.540919666386934E-5 98-99 0.0 0.0 0.0 18.781531320866776 6.540919666386934E-5 100-101 0.0 0.0 0.0 19.810287165996115 6.540919666386934E-5 102-103 0.0 0.0 0.0 20.718788203058928 6.540919666386934E-5 104-105 0.0 0.0 0.0 21.7091815543449 6.540919666386934E-5 106-107 0.0 0.0 0.0 22.67870937189511 6.540919666386934E-5 108-109 0.0 0.0 0.0 23.527295584813814 6.540919666386934E-5 110-111 0.0 0.0 0.0 24.455026925695805 6.540919666386934E-5 112-113 0.0 0.0 0.0 26.38371520312499 6.540919666386934E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTAGTA 1880 0.0 87.03396 2 GAGTAGT 2340 0.0 84.27697 1 GAGTTAG 1230 0.0 78.95493 1 GAGTAAG 1195 0.0 75.284546 1 GAGTCAG 5500 0.0 73.98689 1 GAGTCGT 890 0.0 71.62927 1 AGTCGTA 475 0.0 71.39949 2 GAGTTAC 645 0.0 70.20219 1 AGTTAGT 805 0.0 67.26038 2 GAGTACG 630 0.0 66.19943 1 AGTCCGT 345 0.0 65.53577 2 GAGTCCG 800 0.0 64.792694 1 GTGGGGT 6490 0.0 64.25216 7 TGGGGTA 6740 0.0 64.16363 8 TCGTAGG 1110 0.0 63.251804 2 GAGTAAT 540 0.0 61.786133 1 GTTAGTG 880 0.0 61.523945 3 GAGTAAC 875 0.0 60.60085 1 AGTAGCG 285 0.0 60.543427 2 GGGGTAA 8065 0.0 60.405956 9 >>END_MODULE