FastQCFastQC Report
Thu 26 May 2016
SRR935913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754040
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC167390.9543111901667009No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT165000.9406855031812273No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT153550.8754076303847119No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG153430.8747234954733073No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT149510.852375088367426No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT104660.5966796652299834No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA80490.45888349182458776No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC52920.30170349592939727No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG49580.28266174089530455No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA49100.27992520124968645No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC48490.27644751545004675No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG44590.25421313082939956No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG44500.25370002964584615No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC43500.24799890538414177No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG43420.2475428154432054No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC40800.23260586987753984No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT39220.2235980935440469No Hit
GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT34850.19868418052039866No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT33680.19201386513420446No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC32580.1857426284463296No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT32180.18346217874164786No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC31010.1767918633554537No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG30410.17337118879843105No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG29710.16938040181523797No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA29020.16544662607466193No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG28450.16219698524549042No Hit
GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT27430.15638183849855192No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT27210.15512759116097694No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA26630.1518209390891884No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA25840.1473170509224419No Hit
ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA24150.13768215092016145No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA22850.13027068937994574No Hit
CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA22750.1297005769537753No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA22590.1287883970719026No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC22150.12627990239675263No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA21160.12063578937766528No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA20390.11624592369615287No Hit
GATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACG19470.11100088937538483No Hit
GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG18670.1064399899660213No Hit
GTATCAACGCAGAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC18580.10592688878246789No Hit
GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG18560.10581286629723383No Hit
GAGTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGA18380.10478666393012703No Hit
TTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA18300.10433057398919067No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC18040.10284828168114753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGTA22900.077.818652
GAGTTAG17450.077.3534551
GAGTAGT30400.076.426281
GAGTAAG14600.074.4535141
GAGTCAG73500.073.5407261
AGTTAGT10700.073.525522
AGTTAGG5700.073.193272
TCGTAGG14900.072.000292
GAGTACG8400.071.102911
AGTCGTA4650.070.49492
GAGTTCG6950.070.468611
AGTCGGT4950.068.630582
GATTGTG93650.067.404269
GAGTCGT12600.065.88871
GAGTCGA5100.065.5819851
GAGTAAC11700.065.341751
GAGTAGG16550.064.9592741
GTAGTAG52100.064.426611
GAGTTAC10850.064.405481
GTACACG3450.064.054451
Produced by FastQC (version 0.11.5)