##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935912_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 957470 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.97376419104515 31.0 31.0 34.0 28.0 34.0 2 30.867679405098855 31.0 31.0 34.0 27.0 34.0 3 31.12853666433413 33.0 31.0 34.0 27.0 34.0 4 34.29582754551056 37.0 35.0 37.0 30.0 37.0 5 34.48866178574786 37.0 35.0 37.0 31.0 37.0 6 34.598229709547034 37.0 35.0 37.0 31.0 37.0 7 34.56031520569835 37.0 35.0 37.0 31.0 37.0 8 34.566367614651114 37.0 35.0 37.0 31.0 37.0 9 36.1188569876863 39.0 37.0 39.0 31.0 39.0 10-11 35.83494522021578 39.0 37.0 39.0 30.0 39.0 12-13 35.66027603998037 39.0 37.0 39.0 30.0 39.0 14-15 37.14552414174857 40.0 38.0 41.0 30.5 41.0 16-17 37.34670276875516 40.0 38.0 41.0 31.0 41.0 18-19 37.418056440410666 40.0 38.0 41.0 31.0 41.0 20-21 37.55043656720315 40.0 38.0 41.0 31.0 41.0 22-23 37.49457215369672 40.0 38.0 41.0 31.0 41.0 24-25 37.43419376064002 40.0 38.0 41.0 31.0 41.0 26-27 37.41227662485508 40.0 37.5 41.0 30.5 41.0 28-29 37.311280771199094 40.0 37.0 41.0 30.0 41.0 30-31 37.232005180318964 40.0 37.0 41.0 30.0 41.0 32-33 37.14102843953335 40.0 37.0 41.0 30.0 41.0 34-35 36.85307007008052 40.0 36.0 41.0 30.0 41.0 36-37 36.849130521060715 40.0 36.0 41.0 30.0 41.0 38-39 36.98127617575486 40.0 36.0 41.0 30.0 41.0 40-41 36.95910681274609 40.0 36.0 41.0 30.0 41.0 42-43 36.80136087814762 40.0 35.0 41.0 30.0 41.0 44-45 36.59724273345379 40.0 35.0 41.0 30.0 41.0 46-47 36.427074477529324 40.0 35.0 41.0 30.0 41.0 48-49 36.25782896592061 39.5 35.0 41.0 30.0 41.0 50-51 35.99841979383166 39.0 35.0 41.0 30.0 41.0 52-53 35.72256885333222 39.0 35.0 41.0 29.5 41.0 54-55 35.43886858073883 38.0 35.0 40.0 29.0 41.0 56-57 35.14667195839034 37.5 35.0 40.0 29.0 41.0 58-59 34.759591945439546 37.0 34.0 39.5 28.0 41.0 60-61 34.435994339248225 36.5 34.0 39.0 28.0 41.0 62-63 33.988059155900444 36.0 34.0 39.0 26.5 41.0 64-65 33.52006433621941 35.0 34.0 38.0 26.0 40.0 66-67 33.161424901041286 35.0 33.0 37.0 26.5 39.5 68-69 32.93583036544226 35.0 33.0 36.5 26.5 39.0 70-71 32.77531358684867 35.0 33.5 36.0 27.0 39.0 72-73 32.53575151179672 35.0 33.5 35.5 26.5 37.0 74-75 32.2318197959205 35.0 33.0 35.0 26.0 37.0 76-77 32.06358058215923 35.0 33.0 35.0 26.0 36.5 78-79 31.883889312458876 35.0 33.0 35.0 25.5 36.0 80-81 31.683619852319133 35.0 33.0 35.0 25.0 36.0 82-83 31.56532319550482 35.0 33.0 35.0 25.0 35.0 84-85 31.38101507096828 35.0 33.0 35.0 24.0 35.0 86-87 31.213052628280785 35.0 33.0 35.0 24.0 35.0 88-89 31.099226085412596 35.0 33.0 35.0 23.0 35.0 90-91 31.047219756232572 35.0 33.0 35.0 23.0 35.0 92-93 30.949850125852507 35.0 32.5 35.0 20.0 36.0 94-95 30.76358684867411 35.0 32.0 35.0 19.5 35.0 96-97 30.63896414509071 35.0 32.0 35.0 18.5 35.0 98-99 30.454400137863328 35.0 32.0 35.0 17.5 35.0 100-101 30.322468589094175 35.0 31.5 35.0 16.0 35.0 102-103 29.614961826480204 34.0 30.0 35.0 15.0 35.0 104-105 30.17670109768452 35.0 31.0 35.0 15.0 35.0 106-107 30.196959695865147 35.0 31.5 35.0 11.5 35.0 108-109 30.068945763313735 35.0 31.0 35.0 7.5 35.0 110-111 29.96651539996031 35.0 31.0 35.0 4.5 35.0 112-113 29.869968771867526 35.0 31.0 35.0 2.0 35.0 114-115 29.678448933125843 35.0 31.0 35.0 2.0 35.0 116-117 29.415875171023636 35.0 30.5 35.0 2.0 35.0 118-119 29.17155106687416 35.0 30.0 35.0 2.0 35.0 120-121 28.976241553260152 35.0 30.0 35.0 2.0 35.0 122-123 28.540805456045618 35.0 30.0 35.0 2.0 35.0 124-125 25.61618588571966 31.5 23.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11109374653943505 1101 2 0.2960348046497927 1101 3 0.29008499043989033 1101 4 0.2580585235630224 1101 5 0.09911010808463061 1101 6 0.3162417042466714 1101 7 0.1097751130848792 1101 8 0.15690667843212225 1101 9 0.20482704688667752 1101 10-11 0.13923665656291462 1101 12-13 0.020808194500396837 1101 14-15 0.1734153923248627 1101 16-17 0.09860400556636506 1101 18-19 0.23343502544808103 1101 20-21 0.12778185025384659 1101 22-23 0.2450650272298347 1101 24-25 0.2911327237241963 1101 26-27 0.29088765267350425 1101 28-29 0.2136356842174365 1101 30-31 0.09103553377578777 1101 32-33 0.12696383838672176 1101 34-35 -0.11882502383856064 1101 36-37 -0.01209355801267975 1101 38-39 0.08597073451583981 1101 40-41 0.02659371954001699 1101 42-43 0.07668369439908673 1101 44-45 0.18763431902195293 1101 46-47 0.19023963634519703 1101 48-49 0.18520449923411775 1101 50-51 0.30094428329965695 1101 52-53 0.2487527842531776 1101 54-55 0.1384341196535388 1101 56-57 0.12910969710770104 1101 58-59 0.1458586650874949 1101 60-61 0.1120102645116674 1101 62-63 0.25383795906269313 1101 64-65 0.3313075696558627 1101 66-67 0.17216333583977672 1101 68-69 0.2573044661805568 1101 70-71 0.055000215373986805 1101 72-73 0.1984757394812462 1101 74-75 0.1700845114565368 1101 76-77 0.29213668449670394 1101 78-79 0.3231389021145006 1101 80-81 0.22876573327362948 1101 82-83 0.16507695404693834 1101 84-85 0.06690660473768872 1101 86-87 0.023978252774575992 1101 88-89 -0.08606463975345235 1101 90-91 -0.028292551356429385 1101 92-93 -0.22209809477162068 1101 94-95 -0.181494734208961 1101 96-97 -0.1035403242365156 1101 98-99 -0.31014849287693025 1101 100-101 -0.16462445383759317 1101 102-103 -0.09460203739311268 1101 104-105 -0.2953391473052527 1101 106-107 -0.12834541972870994 1101 108-109 -0.12551925196560632 1101 110-111 -0.2478437997334062 1101 112-113 -0.22812554591477507 1101 114-115 -0.21851304050637665 1101 116-117 -0.4439166828388146 1101 118-119 -0.6445074407138982 1101 120-121 -0.7897070218359268 1101 122-123 -0.7758046372859688 1101 124-125 -0.5253045274509347 1102 1 0.23508999984603207 1102 2 0.12851383540468575 1102 3 0.14524422490946876 1102 4 0.154163092081383 1102 5 0.24979684497826327 1102 6 0.1777034795015453 1102 7 0.25501361177165904 1102 8 0.2820843639546382 1102 9 0.33384791728686025 1102 10-11 0.47714039101836647 1102 12-13 0.499021125299528 1102 14-15 0.5191066949127219 1102 16-17 0.6314464090984231 1102 18-19 0.4856296181599262 1102 20-21 0.510072840303458 1102 22-23 0.5445675077668 1102 24-25 0.5451394734205977 1102 26-27 0.6195882184430346 1102 28-29 0.5369503782217819 1102 30-31 0.48029038423753434 1102 32-33 0.5757762871991687 1102 34-35 0.48738267288637616 1102 36-37 0.41457998072982605 1102 38-39 0.3976711496645322 1102 40-41 0.48684276168560814 1102 42-43 0.43678282173959104 1102 44-45 0.429933828562838 1102 46-47 0.47482947696952493 1102 48-49 0.46930482212754043 1102 50-51 0.5460785241235513 1102 52-53 0.578365312141564 1102 54-55 0.544462012750401 1102 56-57 0.631106599966671 1102 58-59 0.5118014103405883 1102 60-61 0.4949237422527375 1102 62-63 0.5948494250741518 1102 64-65 0.5452350551350733 1102 66-67 0.44731348598993037 1102 68-69 0.6513897740589627 1102 70-71 0.5454216713126883 1102 72-73 0.47877170943238667 1102 74-75 0.5373804908214765 1102 76-77 0.644438829902299 1102 78-79 0.6541376124235612 1102 80-81 0.7578720339316547 1102 82-83 0.6917509634795671 1102 84-85 0.6820515302274401 1102 86-87 0.6532450587655205 1102 88-89 0.6785702929849293 1102 90-91 0.6395708449552409 1102 92-93 0.8126200089119955 1102 94-95 0.8622135508786357 1102 96-97 0.7014892351033915 1102 98-99 0.6852234464605296 1102 100-101 0.768865854652713 1102 102-103 0.8035406193702315 1102 104-105 0.7091041674828915 1102 106-107 0.5812917252532621 1102 108-109 0.7424404004768093 1102 110-111 0.7944806563841524 1102 112-113 0.8438265001752008 1102 114-115 0.8304634438149385 1102 116-117 0.888118722472452 1102 118-119 0.7964091812372054 1102 120-121 0.8807804719274301 1102 122-123 0.8517465031962068 1102 124-125 0.4265062174632632 1103 1 0.06528873790993828 1103 2 0.004138898670198898 1103 3 0.11685403696871788 1103 4 0.1246324378316288 1103 5 0.17088872337351546 1103 6 0.005120222086830495 1103 7 0.08980458251767942 1103 8 0.13179249905603285 1103 9 0.10128895382901959 1103 10-11 0.21233963520637644 1103 12-13 0.14842052469892764 1103 14-15 0.09765546840530703 1103 16-17 0.06436134650774505 1103 18-19 0.043814488423443265 1103 20-21 0.11808394129209177 1103 22-23 0.09456075944544295 1103 24-25 0.125982676173912 1103 26-27 0.022046294496618657 1103 28-29 -0.11863217061919329 1103 30-31 -0.10827880292715975 1103 32-33 -0.02816895017303267 1103 34-35 0.03897527672616974 1103 36-37 -0.059867500459233725 1103 38-39 -0.03622145080110073 1103 40-41 0.005600564432171495 1103 42-43 -0.18219177083050653 1103 44-45 -0.051493103987695577 1103 46-47 0.0045634244001035995 1103 48-49 -0.11856253961622087 1103 50-51 -0.1492415150167119 1103 52-53 -0.11713018335392889 1103 54-55 -0.10653083402896613 1103 56-57 -0.18459505674621823 1103 58-59 -0.21401682362371588 1103 60-61 -0.22486233596031013 1103 62-63 -0.40952601778443665 1103 64-65 -0.43139129031149537 1103 66-67 -0.29459696378569333 1103 68-69 -0.09796070001060997 1103 70-71 0.020640148216550358 1103 72-73 -0.046456046413346996 1103 74-75 -0.07796181777925426 1103 76-77 -0.03104789277544029 1103 78-79 -0.16402633804376165 1103 80-81 -0.1862569264894418 1103 82-83 -0.1048099232498565 1103 84-85 0.010646698429351176 1103 86-87 -0.07912073399353048 1103 88-89 -0.19929858713114257 1103 90-91 -0.25122528825662016 1103 92-93 -0.18471946539376916 1103 94-95 -0.20594702542800647 1103 96-97 -0.2683187751540608 1103 98-99 -0.13579335685964722 1103 100-101 -0.10637624137590151 1103 102-103 0.02605386295089218 1103 104-105 -0.0675677291328256 1103 106-107 -0.14231727700742525 1103 108-109 -0.3041145814714312 1103 110-111 -0.22009909190346022 1103 112-113 -0.0758440130346365 1103 114-115 -0.4104811524667369 1103 116-117 -0.3108040767312481 1103 118-119 -0.3054390152739117 1103 120-121 -0.19009799080496492 1103 122-123 -0.2476680799262425 1103 124-125 -0.146292986234819 1104 1 -0.07474060226943635 1104 2 0.14393207263301377 1104 3 0.10903888009878315 1104 4 0.10105360277010789 1104 5 0.17969855677908697 1104 6 0.06880674894309635 1104 7 0.12547234944107544 1104 8 0.06689291137445963 1104 9 0.10104389664900992 1104 10-11 0.09072987070577199 1104 12-13 0.10143733973355751 1104 14-15 0.15642168052147554 1104 16-17 0.2031731803800909 1104 18-19 0.09945969452709136 1104 20-21 0.1501858761880115 1104 22-23 0.1299670764749763 1104 24-25 0.0878721170434531 1104 26-27 0.1569286003679906 1104 28-29 0.21913553073334668 1104 30-31 0.14650259050909398 1104 32-33 0.23557592363085433 1104 34-35 0.21847021443386438 1104 36-37 0.2690067368628064 1104 38-39 0.11121072781835295 1104 40-41 0.18582067134600777 1104 42-43 0.055527308065677516 1104 44-45 -0.09684146304924468 1104 46-47 0.061653689906499665 1104 48-49 0.015460399069468167 1104 50-51 -0.048391316637086845 1104 52-53 -0.1458641101590672 1104 54-55 -0.034100600307766626 1104 56-57 -0.025517383066798516 1104 58-59 -0.009088173605370287 1104 60-61 0.10505464466849901 1104 62-63 -0.0014560792432192216 1104 64-65 -0.08983881695660045 1104 66-67 0.010442267590519805 1104 68-69 0.08756036601590722 1104 70-71 0.120141420735699 1104 72-73 0.08006625323434946 1104 74-75 0.1091629557790128 1104 76-77 0.14137344998231782 1104 78-79 0.050408141112487925 1104 80-81 0.1476854378400141 1104 82-83 0.1981702165605448 1104 84-85 0.05428021291606555 1104 86-87 0.09319725131415169 1104 88-89 0.16218075804940213 1104 90-91 0.184599181897525 1104 92-93 0.29069340872129956 1104 94-95 0.21374423586629376 1104 96-97 0.18971893364465942 1104 98-99 0.18190521725209052 1104 100-101 0.25930325050553904 1104 102-103 0.3530912827933008 1104 104-105 0.18630206270749383 1104 106-107 0.17852508621065866 1104 108-109 0.19894029800044422 1104 110-111 0.11188337067114063 1104 112-113 0.08562616333984963 1104 114-115 0.06202215386029408 1104 116-117 -0.06081574612077034 1104 118-119 -0.030983864627650348 1104 120-121 -0.015353050318854145 1104 122-123 1.1556959197989158E-4 1104 124-125 0.2799950920945662 1105 1 0.28598265073868134 1105 2 0.2813006881915392 1105 3 0.19952267918792188 1105 4 0.3097252476435344 1105 5 0.4565618517432668 1105 6 0.3766887284867977 1105 7 0.42051207727012496 1105 8 0.449090930961205 1105 9 0.4553655888045327 1105 10-11 0.5814633953413733 1105 12-13 0.6348674111458195 1105 14-15 0.6209299467359699 1105 16-17 0.7071649848170054 1105 18-19 0.7079335154638215 1105 20-21 0.6347197399503628 1105 22-23 0.6058502190495076 1105 24-25 0.5346504129315477 1105 26-27 0.7156298644846828 1105 28-29 0.5481373894087938 1105 30-31 0.5314709604181118 1105 32-33 0.6347976712205465 1105 34-35 0.5937104292141342 1105 36-37 0.619516785666633 1105 38-39 0.6411049680983538 1105 40-41 0.628570478413323 1105 42-43 0.6143345492913213 1105 44-45 0.5370733857023993 1105 46-47 0.5745679517080049 1105 48-49 0.5906731684958828 1105 50-51 0.5564999345449664 1105 52-53 0.6429430767193338 1105 54-55 0.7075902508786385 1105 56-57 0.6526605715206415 1105 58-59 0.5893539628931421 1105 60-61 0.5164925638215578 1105 62-63 0.47561618084091606 1105 64-65 0.49742708232709987 1105 66-67 0.5293977680742117 1105 68-69 0.601051523720713 1105 70-71 0.5403545162455288 1105 72-73 0.627145632806311 1105 74-75 0.6074890609300496 1105 76-77 0.6990010844645482 1105 78-79 0.6187987020846535 1105 80-81 0.49008171614134 1105 82-83 0.5598853066139107 1105 84-85 0.5622127353886484 1105 86-87 0.5328848634053251 1105 88-89 0.7510944655091016 1105 90-91 0.64188497226937 1105 92-93 0.6496674959594877 1105 94-95 0.6139072775723626 1105 96-97 0.6046902383043928 1105 98-99 0.7169950532321323 1105 100-101 0.6971221329089907 1105 102-103 0.5933625841921994 1105 104-105 0.7761907595694844 1105 106-107 0.6631978071593458 1105 108-109 0.6785869866233938 1105 110-111 0.592206954110452 1105 112-113 0.5651733965220949 1105 114-115 0.7456492340007266 1105 116-117 0.516191025544753 1105 118-119 0.5732291330571613 1105 120-121 0.7482894644364215 1105 122-123 0.5331361345858348 1105 124-125 0.661263502220546 1106 1 0.1253014879298604 1106 2 0.10648968359330269 1106 3 0.1421070451765445 1106 4 -0.42172982210940546 1106 5 -0.029565496086206622 1106 6 0.06609719236334399 1106 7 0.2221019767323611 1106 8 0.24688533513858602 1106 9 0.42170492322897246 1106 10-11 0.33938561496572106 1106 12-13 0.1984205246989319 1106 14-15 0.11338182110273465 1106 16-17 0.17930277610373224 1106 18-19 0.006265679753433062 1106 20-21 0.0643744823710577 1106 22-23 0.10468507213968081 1106 24-25 0.09305973410682356 1106 26-27 0.011304402712411843 1106 28-29 -0.044446976579827435 1106 30-31 -0.08335251397983257 1106 32-33 -0.10574086825415918 1106 34-35 0.007438899325578063 1106 36-37 0.026230993444670503 1106 38-39 -0.03418144761359798 1106 40-41 -0.005651328148907453 1106 42-43 -0.11081158117396228 1106 44-45 -0.0015050571313679484 1106 46-47 -0.03434405826784115 1106 48-49 -0.007557678671126666 1106 50-51 0.02015046840826784 1106 52-53 0.01691236983755573 1106 54-55 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-0.47109835984524295 2216 60-61 -0.4031980959668999 2216 62-63 -0.45073527943475256 2216 64-65 -0.29676495220723353 2216 66-67 -0.2774087912995853 2216 68-69 -0.4600398095588787 2216 70-71 -0.274735894566156 2216 72-73 -0.3471110192620799 2216 74-75 -0.19550918369411363 2216 76-77 -0.3821568366689192 2216 78-79 -0.6317543308644709 2216 80-81 -0.5245846281093591 2216 82-83 -0.7435119277735431 2216 84-85 -0.6562608041117883 2216 86-87 -0.7305840637555328 2216 88-89 -0.7247113505167633 2216 90-91 -0.8468192737698388 2216 92-93 -0.6357810204792536 2216 94-95 -0.7772094907448945 2216 96-97 -0.8314942373666341 2216 98-99 -0.7484480000471052 2216 100-101 -0.7781496816660045 2216 102-103 -0.8259877476275719 2216 104-105 -1.1567317173016498 2216 106-107 -0.9988789099295978 2216 108-109 -1.1161321084624518 2216 110-111 -1.296094943665775 2216 112-113 -1.4847352522863098 2216 114-115 -1.36196829109846 2216 116-117 -1.4291854398806088 2216 118-119 -1.5435606785981761 2216 120-121 -1.2942022736543386 2216 122-123 -0.9220746896347656 2216 124-125 -0.7800603735776583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14600.0 3 2935.0 4 1972.0 5 1481.0 6 1815.0 7 2036.0 8 2454.0 9 2854.0 10 3550.0 11 4076.0 12 3921.0 13 3608.0 14 3456.0 15 3490.0 16 3438.0 17 3282.0 18 3262.0 19 3001.0 20 3006.0 21 3241.0 22 3833.0 23 4830.0 24 7096.0 25 10132.0 26 12431.0 27 10996.0 28 12656.0 29 16081.0 30 18568.0 31 22771.0 32 31363.0 33 42440.0 34 69304.0 35 99708.0 36 206440.0 37 284020.0 38 33227.0 39 96.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 73.17187478750637 9.160685461303405 5.819160988836827 11.848278762353399 2 10.466508301628986 34.308699004055235 43.40915216205883 11.815640532256952 3 40.37281029312382 19.611396555379958 17.58753343771576 22.428259713780466 4 14.592456251775834 15.439425214353763 49.48082933596296 20.48728919790744 5 16.922541838436736 32.51287557458329 27.43795127178612 23.12663131519386 6 30.157080639602285 39.20728586796453 14.469800620384973 16.16583287204821 7 46.453973300357404 22.004854480472414 15.911687725726031 15.629484493444155 8 44.21505470678916 22.154474092084538 15.372419757109377 18.258051444016928 9 59.91310432702852 9.904122322370414 9.25209144934045 20.930681901260613 10-11 41.70308208090071 25.426331895516306 18.00004177676585 14.870544246817133 12-13 29.306923454520767 30.221625742843116 23.318328511598274 17.15312229103784 14-15 25.133058999237573 30.172642484882033 21.816610442102625 22.87768807377777 16-17 22.97748232320595 35.072639351624595 20.815325806552686 21.134552518616772 18-19 23.42444149686152 26.657075417506555 24.969241856141707 24.94924122949022 20-21 23.644866157686405 28.38297805675374 21.938859703176078 26.033296082383785 22-23 24.999268906597596 24.3320939559464 22.037087323884823 28.631549813571183 24-25 21.85274734456432 23.130280844308437 33.80179013441674 21.215181676710497 26-27 17.666282490277656 23.054291069230604 31.944223228240986 27.335203212250754 28-29 21.670649701167964 24.259239142923395 22.866604553611154 31.203506602297487 30-31 21.951601616760836 28.635675269199034 21.163848475670257 28.24887463836987 32-33 30.354580300166063 25.174940207003875 18.897406707259755 25.573072785570304 34-35 28.88581365473592 18.577553343707898 25.924102060638976 26.612530940917207 36-37 24.939371468557763 25.729265668898243 28.837561490177237 20.493801372366757 38-39 22.55981910660386 30.194157519295644 24.296061495399336 22.94996187870116 40-41 20.39390268102395 29.721244529854722 26.107397620813185 23.777455168308144 42-43 21.23852444463012 22.49073078007666 23.75839451888832 32.5123502564049 44-45 23.162187849227653 23.723928687060692 22.681911704805373 30.431971758906286 46-47 25.156453988114507 21.984918587527545 23.41979383166052 29.43883359269742 48-49 17.469946839065454 22.57788755783471 26.010893291695826 33.94127231140401 50-51 22.826198209865584 24.80270922326548 24.700565030758142 27.67052753611079 52-53 25.039896811388346 21.714100702894086 28.340052429841144 24.905950055876424 54-55 20.85887808495305 24.039813257856643 28.695154939580352 26.406153717609953 56-57 20.414395699484004 26.466722334410658 26.065559473882377 27.053322492222964 58-59 23.068137905104077 22.969544737694132 31.078623873332845 22.883693483868946 60-61 24.972949544111042 29.02681023948531 27.58378852601126 18.41645169039239 62-63 26.29199870492026 25.552915496046875 25.72242472349003 22.432661075542836 64-65 31.15641789423551 19.352069250758266 28.085493938051986 21.406018916954235 66-67 30.961315718837895 23.83628086131248 23.474545912191413 21.72785750765821 68-69 23.41121229323656 28.71633672079043 24.764402089190096 23.10804889678292 70-71 24.768917625146443 28.457774223758044 22.074745492085196 24.69856265901032 72-73 22.918026588850395 28.67325208440169 22.89875703547582 25.50996429127209 74-75 20.7022830597126 29.122315909868362 25.895636778595694 24.27976425182334 76-77 22.61755372120069 28.578806673653855 22.70988071123115 26.093758893914305 78-79 23.44422598392096 30.25251773577417 19.144452886942762 27.158803393362106 80-81 21.771189963423986 22.988945729212144 24.798737908085787 30.44112639927809 82-83 24.006287403260675 18.50569730644302 28.749255851358267 28.738759438938033 84-85 25.90808402796893 19.866987228872745 32.274321995787005 21.950606747371317 86-87 28.416641774053055 22.993254972081974 24.541801835299328 24.048301418565643 88-89 23.448112685237223 25.833658906533678 26.7982086858055 23.920019722423604 90-91 19.84419338902878 28.255588431583682 26.059016009917812 25.841202169469717 92-93 23.81502020709531 22.74846788539459 27.102629481572322 26.333882425937784 94-95 22.31997689403376 21.92042752304982 28.639385540073235 27.120210042843183 96-97 22.17959551162672 20.99039384504187 28.284261552296986 28.545749091034427 98-99 21.102232100426242 23.235997280244487 30.115756721838157 25.54601389749112 100-101 19.59364402309859 28.08161376808037 26.931645257613624 25.39309695120741 102-103 20.830950750911647 26.90336350139613 26.13644612178247 26.129239625909754 104-105 21.1523357536158 23.588458678341954 25.797941228077505 29.46126433996474 106-107 20.564161105929564 25.602873027196 23.121038333836403 30.711927533038036 108-109 22.955340637304563 26.018883098164956 25.03263809832162 25.993138166208862 110-111 24.395072430467795 27.34200549364471 22.572874345932508 25.690047729954983 112-113 21.849481569678026 27.613862756310333 24.20927299645154 26.327382677560102 114-115 23.04270420816963 23.488228415890138 26.19918394546128 27.269883430478952 116-117 25.392597157091085 23.690559495336668 25.02590159482804 25.89094175274421 118-119 21.98719811358225 24.701014074967546 26.641156267050192 26.67063154440001 120-121 19.877205204234997 25.761318333784182 27.39012231435342 26.971354147627398 122-123 21.285116023293963 26.809937796077044 28.58596920563747 23.318976974991525 124-125 21.997777129775102 28.468130396520053 23.64220898644761 25.89188348725724 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7.0 1 7.0 2 12.0 3 17.0 4 21.0 5 21.5 6 15.0 7 24.0 8 38.5 9 38.0 10 43.0 11 59.5 12 85.0 13 109.5 14 143.0 15 190.5 16 239.5 17 301.5 18 379.0 19 459.5 20 554.5 21 652.5 22 807.0 23 1038.5 24 1231.0 25 1468.0 26 1804.0 27 2098.0 28 2349.0 29 2720.0 30 3218.0 31 3635.5 32 4177.5 33 4863.0 34 5654.0 35 6109.0 36 6566.0 37 7457.5 38 7910.5 39 8051.5 40 8355.5 41 8917.5 42 9476.0 43 10252.0 44 11353.5 45 14894.5 46 29306.5 47 57726.5 48 82867.5 49 99696.0 50 102792.0 51 87471.5 52 66832.5 53 49087.5 54 38079.0 55 32238.0 56 28104.5 57 23843.0 58 20185.5 59 16263.5 60 13793.5 61 12279.0 62 9915.5 63 7385.0 64 5714.0 65 5114.0 66 4633.5 67 4143.5 68 3847.0 69 3652.0 70 3184.5 71 2326.0 72 1768.5 73 1513.5 74 1127.5 75 918.0 76 741.5 77 569.5 78 573.5 79 477.5 80 409.5 81 392.5 82 269.0 83 133.5 84 66.0 85 59.0 86 45.0 87 27.0 88 16.0 89 11.0 90 8.5 91 9.0 92 9.5 93 5.5 94 2.0 95 1.5 96 3.0 97 3.5 98 1.5 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16282494490689003 2 0.01963507995028565 3 0.009713098060513646 4 0.018172893145477143 5 0.003864350841279622 6 0.0 7 4.177676585167159E-4 8 2.0888382925835796E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0010966401036063794 28-29 0.007937585511817603 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.6554670120212643E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.002297722121841938 66-67 1.0444191462917898E-4 68-69 0.006840945408211223 70-71 0.018956207505195985 72-73 1.0444191462917898E-4 74-75 0.009660877103199055 76-77 3.6554670120212643E-4 78-79 0.0013055239328647373 80-81 0.0012533029755501478 82-83 0.0 84-85 0.020209510480746134 86-87 0.0025066059511002956 88-89 0.019217312291768932 90-91 1.0444191462917898E-4 92-93 0.013890774645680806 94-95 0.014778530920028825 96-97 0.0065798406216382764 98-99 0.0032376993535045482 100-101 9.399772316626109E-4 102-103 5.222095731458949E-5 104-105 6.266514877750739E-4 106-107 0.0013577448901793267 108-109 0.0 110-111 0.0 112-113 0.0013055239328647373 114-115 0.00610985200580697 116-117 0.0 118-119 0.07686924916707573 120-121 0.0019843963779544007 122-123 0.005692084347290254 124-125 0.25050393223808576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 957470.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.63719011058514 #Duplication Level Percentage of deduplicated Percentage of total 1 82.63015484794906 32.75227156575245 2 8.876310701188023 7.0366402948722175 3 2.659070071380534 3.161941978100323 4 1.316841969444508 2.0878366195387725 5 0.7710953563971508 1.5282026617451634 6 0.5650628083934195 1.3438501176426667 7 0.3918925715845425 1.0873464253975826 8 0.31496073763199006 0.9987326907911453 9 0.25687027199575785 0.9163454224368213 >10 1.846890965991877 14.680868523251748 >50 0.19569707979970621 5.385759618749892 >100 0.15118436522069398 11.871251546504276 >500 0.013169369792693727 3.5100109774357615 >1k 0.008955171459031734 6.701081901450559 >5k 7.901621875616236E-4 2.146450899618669 >10k+ 0.0010535495834154982 4.791408756712004 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 12832 1.3401986485216246 No Hit GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 12013 1.254660720440327 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 10526 1.099355593386738 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 10524 1.0991467095574796 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 8232 0.8597658412274014 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 7237 0.7558461361713683 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 5091 0.5317137873771501 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 4300 0.4491002329054696 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 3916 0.4089945376878649 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3372 0.35217813612959153 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3368 0.3517603684710748 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 3332 0.3480004595444243 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 2573 0.2687290463408775 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2368 0.2473184538418958 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 2284 0.23854533301304479 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 2280 0.2381275653545281 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2236 0.2335321211108442 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 2221 0.2319654923914065 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 2196 0.22935444452567705 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 1838 0.19196423908843097 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1763 0.18413109549124254 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1700 0.17755125486960427 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 1687 0.17619350997942496 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1581 0.165122667028732 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1547 0.16157164193133988 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1423 0.14862084451732172 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1413 0.14757642537102988 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1412 0.14747198345640072 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1399 0.14611423856622138 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1395 0.14569647090770468 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1372 0.14329430687123357 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1351 0.1411010266640208 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1317 0.13755000156662872 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1158 0.12094373714058926 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1118 0.1167660605554221 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA 1103 0.11519943183598443 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 1050 0.10966401036063794 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1050 0.10966401036063794 No Hit ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA 1043 0.10893291695823369 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 1013 0.10579965951935831 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCACGT 1008 0.10527744994621242 No Hit GAGTAAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 962 0.10047312187327019 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.0444191462917898E-4 0.0 0.0 0.0 0.0 3 1.0444191462917898E-4 0.0 0.0 0.0 0.0 4 1.0444191462917898E-4 0.0 0.0 0.0 0.0 5 1.0444191462917898E-4 0.0 0.0 0.0 0.0 6 1.0444191462917898E-4 0.0 0.0 0.0 0.0 7 1.0444191462917898E-4 0.0 0.0 0.0 0.0 8 1.0444191462917898E-4 0.0 0.0 0.0 0.0 9 1.0444191462917898E-4 0.0 0.0 0.0 0.0 10-11 1.0444191462917898E-4 0.0 0.0 5.222095731458949E-5 0.0 12-13 1.0444191462917898E-4 0.0 0.0 1.0444191462917898E-4 0.0 14-15 1.0444191462917898E-4 0.0 0.0 1.0444191462917898E-4 0.0 16-17 1.0444191462917898E-4 0.0 0.0 1.5666287194376848E-4 0.0 18-19 1.0444191462917898E-4 0.0 0.0 3.1332574388753695E-4 0.0 20-21 1.0444191462917898E-4 0.0 0.0 9.921981889772003E-4 0.0 22-23 1.0444191462917898E-4 0.0 0.0 0.002976594566931601 0.0 24-25 1.0444191462917898E-4 0.0 0.0 0.004699886158313054 0.0 26-27 1.0444191462917898E-4 0.0 0.0 0.0076242597679300654 0.0 28-29 1.0444191462917898E-4 0.0 0.0 0.014204100389568344 0.0 30-31 1.0444191462917898E-4 0.0 0.0 0.04245563829676126 0.0 32-33 1.0444191462917898E-4 0.0 0.0 0.10141309910493279 0.0 34-35 1.0444191462917898E-4 0.0 0.0 0.1715458447784265 0.0 36-37 1.0444191462917898E-4 0.0 0.0 0.25614379562806144 0.0 38-39 1.0444191462917898E-4 0.0 0.0 0.39296270379228593 0.0 40-41 1.0444191462917898E-4 0.0 0.0 0.6129173760013369 0.0 42-43 1.0444191462917898E-4 0.0 0.0 1.1270327007634704 0.0 44-45 1.0444191462917898E-4 0.0 0.0 1.853582879881354 0.0 46-47 1.0444191462917898E-4 0.0 0.0 2.5946504851326937 0.0 48-49 1.0444191462917898E-4 0.0 0.0 2.8617606817968184 0.0 50-51 1.0444191462917898E-4 0.0 0.0 3.197175890628427 0.0 52-53 1.0444191462917898E-4 0.0 0.0 3.467314902816798 0.0 54-55 1.0444191462917898E-4 0.0 0.0 3.7458092681755044 0.0 56-57 1.0444191462917898E-4 0.0 0.0 4.197886095647906 0.0 58-59 1.0444191462917898E-4 0.0 0.0 4.713463607214848 0.0 60-61 1.0444191462917898E-4 0.0 0.0 5.445758091637336 0.0 62-63 1.0444191462917898E-4 0.0 0.0 6.334819889918222 0.0 64-65 1.0444191462917898E-4 0.0 0.0 7.154114489226816 0.0 66-67 1.0444191462917898E-4 0.0 0.0 7.822229417109675 0.0 68-69 1.0444191462917898E-4 0.0 0.0 8.350392179389432 0.0 70-71 1.0444191462917898E-4 0.0 0.0 8.952552038183963 0.0 72-73 1.0444191462917898E-4 0.0 0.0 9.51549395803524 0.0 74-75 1.0444191462917898E-4 0.0 0.0 10.153007404931747 0.0 76-77 1.0444191462917898E-4 0.0 0.0 10.964938849258985 0.0 78-79 1.0444191462917898E-4 0.0 0.0 11.989148485070029 0.0 80-81 1.0444191462917898E-4 0.0 0.0 14.310526700575476 0.0 82-83 1.0444191462917898E-4 0.0 0.0 15.898357128682882 0.0 84-85 1.0444191462917898E-4 0.0 0.0 16.594619152558305 0.0 86-87 1.0444191462917898E-4 5.222095731458949E-5 0.0 17.1855515055302 0.0 88-89 1.0444191462917898E-4 1.0444191462917898E-4 0.0 17.8706382445403 0.0 90-91 1.0444191462917898E-4 1.0444191462917898E-4 0.0 18.760744461967477 0.0 92-93 1.0444191462917898E-4 1.0444191462917898E-4 0.0 19.93206053453372 0.0 94-95 1.0444191462917898E-4 1.0444191462917898E-4 0.0 21.472578775314112 0.0 96-97 1.0444191462917898E-4 1.0444191462917898E-4 0.0 23.186888362037454 0.0 98-99 1.0444191462917898E-4 1.0444191462917898E-4 0.0 24.88119732210931 0.0 100-101 1.0444191462917898E-4 1.0444191462917898E-4 0.0 26.34489853467994 0.0 102-103 1.0444191462917898E-4 1.0444191462917898E-4 0.0 27.561437956280614 0.0 104-105 1.0444191462917898E-4 1.0444191462917898E-4 0.0 28.793956990819556 0.0 106-107 1.0444191462917898E-4 1.0444191462917898E-4 0.0 30.01080973816412 0.0 108-109 1.0444191462917898E-4 1.0444191462917898E-4 0.0 31.003060148098633 0.0 110-111 1.0444191462917898E-4 1.0444191462917898E-4 0.0 32.051134761402444 0.0 112-113 1.0444191462917898E-4 1.0444191462917898E-4 0.0 35.32095000365547 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTAGT 2135 0.0 93.19913 1 GAGTCGA 360 0.0 92.67205 1 AGTAGTA 1805 0.0 92.28022 2 GAGTCAG 4935 0.0 88.72856 1 GAGTCCG 660 0.0 88.45969 1 GAGTTAG 1135 0.0 88.181335 1 GAGTACG 660 0.0 87.55704 1 AGTTCGG 180 0.0 85.92679 2 GAGTCGT 665 0.0 85.10699 1 GAGTCGG 650 0.0 83.404854 1 AGTCCGT 265 0.0 83.05841 2 AGTCGGT 280 0.0 82.85798 2 GAGTAAG 965 0.0 82.1084 1 AGTTTCG 95 0.0 81.40433 2 GAGTGCG 235 0.0 81.12325 1 AGTCGAG 125 0.0 80.90338 2 AGTCGAC 150 0.0 79.31704 2 GAGTAGG 925 0.0 79.218506 1 GAGTGGT 925 0.0 78.574455 1 AGTCGTA 430 0.0 77.47245 2 >>END_MODULE