##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935912_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 957470 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.532866826114656 33.0 31.0 34.0 31.0 34.0 2 32.60617042831629 34.0 31.0 34.0 31.0 34.0 3 32.81160871881103 34.0 31.0 34.0 31.0 34.0 4 36.018986495660435 37.0 35.0 37.0 35.0 37.0 5 36.07631675143869 37.0 35.0 37.0 35.0 37.0 6 36.206398111690184 37.0 37.0 37.0 35.0 37.0 7 36.24469800620385 37.0 37.0 37.0 35.0 37.0 8 36.24347812464098 37.0 37.0 37.0 35.0 37.0 9 38.07299967623006 39.0 39.0 39.0 35.0 39.0 10-11 37.761097997848495 39.0 38.0 39.0 35.0 39.0 12-13 37.67685097183201 39.0 37.5 39.0 35.0 39.0 14-15 39.22359133967644 41.0 39.0 41.0 35.5 41.0 16-17 39.29778060931413 41.0 39.0 41.0 35.5 41.0 18-19 39.28724659780463 41.0 39.0 41.0 35.0 41.0 20-21 39.28645649472046 41.0 39.0 41.0 35.0 41.0 22-23 39.136984970808484 41.0 39.0 41.0 35.0 41.0 24-25 38.913994694350734 41.0 38.0 41.0 35.0 41.0 26-27 38.80682110144443 40.5 38.0 41.0 35.0 41.0 28-29 38.6851217270515 40.0 38.0 41.0 35.0 41.0 30-31 38.55800286170846 40.0 38.0 41.0 35.0 41.0 32-33 38.39926629554973 40.0 37.5 41.0 34.5 41.0 34-35 37.96537489425256 40.0 37.0 41.0 34.0 41.0 36-37 37.709399250107055 40.0 37.0 41.0 33.5 41.0 38-39 37.293643142866095 40.0 37.0 41.0 33.0 41.0 40-41 37.01686528037432 40.0 37.0 41.0 32.0 41.0 42-43 36.83318432953513 40.0 36.0 41.0 32.0 41.0 44-45 36.65811252571882 40.0 35.5 41.0 31.0 41.0 46-47 36.47502428274515 40.0 35.0 41.0 31.0 41.0 48-49 36.26756347457362 40.0 35.0 41.0 31.0 41.0 50-51 36.00507274379354 39.5 35.0 41.0 30.0 41.0 52-53 35.72647445872978 39.0 35.0 41.0 29.5 41.0 54-55 35.453408461883924 39.0 35.0 41.0 29.0 41.0 56-57 35.197903328563825 38.0 35.0 40.5 29.0 41.0 58-59 34.8719254911381 37.0 35.0 40.0 28.5 41.0 60-61 34.55622264927361 37.0 35.0 39.0 28.0 41.0 62-63 34.13860382048524 36.0 35.0 39.0 27.5 41.0 64-65 33.6940708325065 35.5 34.0 38.5 26.5 41.0 66-67 33.253375562680816 35.0 34.0 37.0 26.0 39.5 68-69 32.90890419543171 35.0 34.0 37.0 26.0 39.0 70-71 32.74074540194471 35.0 34.0 36.0 26.0 39.0 72-73 32.473371489446144 35.0 34.0 35.5 26.0 37.5 74-75 32.18085736367719 35.0 34.0 35.0 25.5 37.0 76-77 32.046186825696886 35.0 34.0 35.0 25.0 36.5 78-79 32.07133121664386 35.0 34.0 35.0 26.0 36.0 80-81 32.00528946076639 35.0 34.0 35.0 26.0 36.0 82-83 31.909692209677587 35.0 34.0 35.0 26.0 35.5 84-85 31.73307153226733 35.0 33.0 35.0 25.0 35.0 86-87 31.6400007310934 35.0 33.0 35.0 25.0 35.0 88-89 31.57631048492381 35.0 33.0 35.0 25.0 35.0 90-91 31.584494553354155 35.0 34.0 35.0 24.5 35.0 92-93 31.598991091104683 35.0 33.5 35.0 25.0 36.0 94-95 31.496945073997097 35.0 33.0 35.0 24.0 35.0 96-97 31.468548884038142 35.0 33.0 35.0 24.5 35.0 98-99 31.377135053839808 35.0 33.0 35.0 24.0 35.0 100-101 31.330942483837614 35.0 33.0 35.0 24.0 35.0 102-103 30.643180987393862 34.5 31.5 35.0 21.5 35.0 104-105 31.217099230263088 35.0 33.0 35.0 24.0 35.0 106-107 31.313652124870753 35.0 33.0 35.0 24.0 35.0 108-109 31.243704241386155 35.0 33.0 35.0 23.5 35.0 110-111 31.14804119189113 35.0 33.0 35.0 23.0 35.0 112-113 31.096356021598588 35.0 33.0 35.0 21.5 35.0 114-115 30.986548403605333 35.0 33.0 35.0 19.5 35.0 116-117 30.838772494177363 35.0 33.0 35.0 18.5 35.0 118-119 30.61814678266682 35.0 33.0 35.0 7.5 35.0 120-121 30.305163086049696 35.0 33.0 35.0 2.0 35.0 122-123 29.924284833989578 35.0 33.0 35.0 2.0 35.0 124-125 27.607811733004688 32.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03150129269896951 1101 2 0.09066215628765661 1101 3 0.09919946673904434 1101 4 0.11719767810683379 1101 5 0.20588751077747958 1101 6 0.2028747286512882 1101 7 0.2341372908877375 1101 8 0.24459150140436492 1101 9 0.23779725566673449 1101 10-11 0.1882786229010378 1101 12-13 0.2141338625114244 1101 14-15 0.3039932086968875 1101 16-17 0.22923023654227137 1101 18-19 0.2512914886860713 1101 20-21 0.32649976497138056 1101 22-23 0.18591487225919678 1101 24-25 0.36020298248028126 1101 26-27 0.4205857266558368 1101 28-29 0.4443815439792047 1101 30-31 0.317089685159182 1101 32-33 0.37727866620240746 1101 34-35 0.4499561933163392 1101 36-37 0.38910024827354306 1101 38-39 0.3936361219233504 1101 40-41 0.3327274461530507 1101 42-43 0.299869088515635 1101 44-45 0.32846043734210184 1101 46-47 0.3536232224275011 1101 48-49 0.3498350607826026 1101 50-51 0.24418690544659682 1101 52-53 0.22075705326197692 1101 54-55 0.32008226420270347 1101 56-57 0.20284215543636464 1101 58-59 0.2979994927092591 1101 60-61 0.23239704501155956 1101 62-63 0.1775618451333898 1101 64-65 0.21299656777460996 1101 66-67 -0.02221156452968387 1101 68-69 0.11475056217791035 1101 70-71 0.2285212083496191 1101 72-73 0.2175633586657142 1101 74-75 0.28494047858399796 1101 76-77 0.2902310896715363 1101 78-79 0.11720457549679253 1101 80-81 0.2088849419693446 1101 82-83 0.2716629326077502 1101 84-85 0.3475074576445891 1101 86-87 0.12855366878788033 1101 88-89 0.10998937170314704 1101 90-91 0.11989911796774422 1101 92-93 0.2389967237973316 1101 94-95 0.1828139051407689 1101 96-97 0.2586654519196614 1101 98-99 0.2750188227939816 1101 100-101 0.17776325019011097 1101 102-103 0.16413953455035113 1101 104-105 0.19051366081495757 1101 106-107 0.1681646408039299 1101 108-109 0.2176671349412942 1101 110-111 0.1994415536483629 1101 112-113 0.19915979243734938 1101 114-115 0.19178112518996215 1101 116-117 0.1364469650291582 1101 118-119 -0.008688253814867153 1101 120-121 0.16456112824524283 1101 122-123 0.1555555424118218 1101 124-125 0.21908170199758104 1102 1 0.1626567428199408 1102 2 0.18625226049844912 1102 3 0.1924196461661154 1102 4 0.26572394732276194 1102 5 0.2616266354821164 1102 6 0.23953207565346446 1102 7 0.20838818755242983 1102 8 0.13883449047494167 1102 9 0.18084375043736856 1102 10-11 0.22664692851072488 1102 12-13 0.2995111677163109 1102 14-15 0.28658002749060785 1102 16-17 0.2421106718365067 1102 18-19 0.1590632886992509 1102 20-21 0.21278103056299358 1102 22-23 0.18759523600852646 1102 24-25 0.23799417492664077 1102 26-27 0.3279374357316769 1102 28-29 0.21142405671136544 1102 30-31 0.2001890854999573 1102 32-33 0.32604169255164095 1102 34-35 0.2916313324052737 1102 36-37 0.25624325334758424 1102 38-39 0.3971992461761289 1102 40-41 0.5266247133261786 1102 42-43 0.6242216542475063 1102 44-45 0.567939336303759 1102 46-47 0.5687297866719945 1102 48-49 0.6275622393718479 1102 50-51 0.40939678617371555 1102 52-53 0.358325656347823 1102 54-55 0.43932807741403224 1102 56-57 0.360797524598631 1102 58-59 0.3180367713672254 1102 60-61 0.36830058177717007 1102 62-63 0.33630679698178767 1102 64-65 0.4354670790382187 1102 66-67 0.41560438621730356 1102 68-69 0.41721603705717314 1102 70-71 0.46278486071671665 1102 72-73 0.4851342210641647 1102 74-75 0.5238604330211913 1102 76-77 0.32524337468382214 1102 78-79 0.4950699016379829 1102 80-81 0.47693590829617705 1102 82-83 0.5508574876643699 1102 84-85 0.5228477666055866 1102 86-87 0.430400186151644 1102 88-89 0.4815227389261665 1102 90-91 0.41204353511215785 1102 92-93 0.412064776175729 1102 94-95 0.45951615598094975 1102 96-97 0.4049429990592799 1102 98-99 0.4214399304219896 1102 100-101 0.4623460304625482 1102 102-103 0.4871506334235214 1102 104-105 0.5600654226081012 1102 106-107 0.5107305609560555 1102 108-109 0.5875930953844986 1102 110-111 0.4773017021981687 1102 112-113 0.43896346758585025 1102 114-115 0.5153229271800441 1102 116-117 0.6455677629430561 1102 118-119 0.6399203861730847 1102 120-121 0.807756831785774 1102 122-123 0.7505749348450053 1102 124-125 0.6688861302502858 1103 1 0.07717069497434181 1103 2 0.14399595925338105 1103 3 0.1339505028655168 1103 4 0.13472553317827618 1103 5 0.11030733865720066 1103 6 0.17035503344271063 1103 7 0.156414303556069 1103 8 0.2001261191823005 1103 9 0.19298904179613885 1103 10-11 0.1914392489210215 1103 12-13 0.07518431277147641 1103 14-15 0.12772960403344058 1103 16-17 0.16747592472984962 1103 18-19 0.20337107963625556 1103 20-21 0.08993037091457978 1103 22-23 0.15987228834175227 1103 24-25 0.12338321565275834 1103 26-27 0.18715817332320484 1103 28-29 0.22245587504656328 1103 30-31 0.24748047801606532 1103 32-33 0.2052399919746577 1103 34-35 0.26924546339019173 1103 36-37 0.20228958057368374 1103 38-39 0.09179046661004975 1103 40-41 0.03990372267259801 1103 42-43 -0.06604557927815335 1103 44-45 -0.16074946485695563 1103 46-47 -0.026977437406017657 1103 48-49 -0.05059934918299547 1103 50-51 -0.08641081918445082 1103 52-53 -0.004146085056504489 1103 54-55 -0.1058434986564194 1103 56-57 -0.08138876281238083 1103 58-59 0.05195467803794429 1103 60-61 5.870817603010892E-4 1103 62-63 0.03703814630864599 1103 64-65 0.0032455316481332375 1103 66-67 0.03689196593184363 1103 68-69 0.04637384655206489 1103 70-71 0.006434690321604819 1103 72-73 -0.035448159068778295 1103 74-75 0.034194643636304534 1103 76-77 0.03108910805764964 1103 78-79 0.06974176451209502 1103 80-81 0.06603568222740819 1103 82-83 0.14112978243105445 1103 84-85 0.016971665785668222 1103 86-87 0.09495968947518918 1103 88-89 -0.02693459481206517 1103 90-91 -0.02847507338397648 1103 92-93 -0.04682360658905793 1103 94-95 -0.05130224669138528 1103 96-97 0.029494235689163872 1103 98-99 -0.04148141789261928 1103 100-101 0.04026046174776354 1103 102-103 0.13544499520102704 1103 104-105 -0.05189091575272897 1103 106-107 -0.05973034939249544 1103 108-109 0.02497373445052986 1103 110-111 0.04138207470550981 1103 112-113 -0.054810362423932446 1103 114-115 0.029488497244489764 1103 116-117 0.06351070588599939 1103 118-119 0.046004841414841735 1103 120-121 0.2980611810788858 1103 122-123 0.32012060410977483 1103 124-125 0.49394433452197006 1104 1 -0.035657298224073486 1104 2 0.019438562527476222 1104 3 0.08314642635877334 1104 4 0.07825457884448639 1104 5 0.09887189260868467 1104 6 0.11549112075196888 1104 7 0.09237380521756933 1104 8 0.04071114457710934 1104 9 0.13173975029479124 1104 10-11 0.1724837148519569 1104 12-13 0.07816556395230378 1104 14-15 0.07563911625295816 1104 16-17 0.1892673982959039 1104 18-19 0.19304311422210674 1104 20-21 0.13486557801846288 1104 22-23 0.24220399907425616 1104 24-25 0.23339721892018872 1104 26-27 0.253865960695812 1104 28-29 0.20379290924343252 1104 30-31 0.23682215774133653 1104 32-33 0.08115437199666076 1104 34-35 0.05296610931868884 1104 36-37 0.03877281555072898 1104 38-39 0.19034179525339567 1104 40-41 0.17243075675833808 1104 42-43 0.05186554442849456 1104 44-45 0.08517652898605377 1104 46-47 0.10178955266944456 1104 48-49 0.03729727113952919 1104 50-51 0.11712417231356653 1104 52-53 0.13901398562665435 1104 54-55 0.1986344906521964 1104 56-57 0.12659893431695224 1104 58-59 0.19872086792669563 1104 60-61 0.1302045480579892 1104 62-63 0.0977117479267946 1104 64-65 0.09106741230917237 1104 66-67 0.08059021774616326 1104 68-69 -0.002835691228973758 1104 70-71 0.08342293202245088 1104 72-73 0.1677159125479335 1104 74-75 0.20839028928696024 1104 76-77 0.19514072010045425 1104 78-79 0.2638609478770988 1104 80-81 0.25454994666689146 1104 82-83 0.22746881709469946 1104 84-85 0.32254768907998965 1104 86-87 0.35794494158957235 1104 88-89 0.36835541730187416 1104 90-91 0.341574327031676 1104 92-93 0.33259992556844864 1104 94-95 0.35254754048117576 1104 96-97 0.21983281469093185 1104 98-99 0.24945674545660523 1104 100-101 0.38241335297794166 1104 102-103 0.3489378657597122 1104 104-105 0.30106755233426696 1104 106-107 0.30362248849190365 1104 108-109 0.38555314742466607 1104 110-111 0.3438340357186327 1104 112-113 0.4563944742601862 1104 114-115 0.4574783615461584 1104 116-117 0.4710527434725478 1104 118-119 0.5496543040672393 1104 120-121 0.7507343048077573 1104 122-123 0.7238675895174822 1104 124-125 0.5427098798158738 1105 1 0.11184164200484048 1105 2 0.12139234563853307 1105 3 0.14288164662811909 1105 4 0.24981793141674302 1105 5 0.20146262531810777 1105 6 0.12754316366454788 1105 7 0.17524790441214577 1105 8 0.16745541909586592 1105 9 0.22945444904806322 1105 10-11 0.20758115944494904 1105 12-13 0.14109368429883062 1105 14-15 0.23623682714740823 1105 16-17 0.28276176248759555 1105 18-19 0.24892402855999052 1105 20-21 0.3035781463601097 1105 22-23 0.3442901177034017 1105 24-25 0.29362645555892186 1105 26-27 0.3661473489415954 1105 28-29 0.43047332576064434 1105 30-31 0.4335034438143168 1105 32-33 0.4088453753553267 1105 34-35 0.3823005730745166 1105 36-37 0.33330575541008045 1105 38-39 0.35036559934248146 1105 40-41 0.42516116186262565 1105 42-43 0.4498082298340904 1105 44-45 0.4611297794942004 1105 46-47 0.416011408953608 1105 48-49 0.5317359185455288 1105 50-51 0.4654885922655225 1105 52-53 0.45437142739359615 1105 54-55 0.5859620740480338 1105 56-57 0.532588271389379 1105 58-59 0.5345821629126206 1105 60-61 0.521873660350245 1105 62-63 0.35150083717582703 1105 64-65 0.5140946076657471 1105 66-67 0.5658667364796486 1105 68-69 0.5323680022091466 1105 70-71 0.49355244148430444 1105 72-73 0.595938431868376 1105 74-75 0.540166574327337 1105 76-77 0.49069729013774577 1105 78-79 0.5782613348294134 1105 80-81 0.5984964798567418 1105 82-83 0.5059114427183253 1105 84-85 0.5779462717041 1105 86-87 0.6091960149474716 1105 88-89 0.6213059287093543 1105 90-91 0.5288818019504227 1105 92-93 0.5679066070175587 1105 94-95 0.588550410015209 1105 96-97 0.5188879880042663 1105 98-99 0.5214399304219874 1105 100-101 0.5137551318716476 1105 102-103 0.5396800859529733 1105 104-105 0.48198569452837603 1105 106-107 0.5113031115286084 1105 108-109 0.504028761820166 1105 110-111 0.354605929502398 1105 112-113 0.4762757798981667 1105 114-115 0.5516023384594533 1105 116-117 0.6268806692559608 1105 118-119 0.6379791592318576 1105 120-121 0.5975878716168133 1105 122-123 0.5848446441147175 1105 124-125 0.8000068113709666 1106 1 -0.01764164066993601 1106 2 0.10702090147985643 1106 3 0.10414733129805853 1106 4 0.19554312820577024 1106 5 0.19262465477800816 1106 6 0.19191850463563753 1106 7 0.15235023470596332 1106 8 0.1519071375901362 1106 9 0.09046294409911582 1106 10-11 0.11561248981670502 1106 12-13 0.17547915698217054 1106 14-15 0.19034017061245123 1106 16-17 0.2523432448350391 1106 18-19 0.3317040145314678 1106 20-21 0.2633864231896581 1106 22-23 0.18811756685000347 1106 24-25 0.2066846739362873 1106 26-27 0.264144387364162 1106 28-29 0.280512293884712 1106 30-31 0.24946868424764546 1106 32-33 0.4224764251565887 1106 34-35 0.5157352235304415 1106 36-37 0.4660078051334082 1106 38-39 0.46972496338269565 1106 40-41 0.4648618866697305 1106 42-43 0.48012543036405475 1106 44-45 0.5351107630496514 1106 46-47 0.3478093981984145 1106 48-49 0.33466242466352725 1106 50-51 0.4980620471794026 1106 52-53 0.5825142066002087 1106 54-55 0.4883313359917594 1106 56-57 0.5925095557787401 1106 58-59 0.5600868001193788 1106 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-1.5530736045456344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 16.0 4 41.0 5 108.0 6 340.0 7 670.0 8 946.0 9 1748.0 10 3895.0 11 12999.0 12 18440.0 13 5427.0 14 4150.0 15 5165.0 16 3308.0 17 2028.0 18 1770.0 19 1756.0 20 1748.0 21 1890.0 22 2088.0 23 2376.0 24 3013.0 25 4102.0 26 5317.0 27 6816.0 28 8819.0 29 11523.0 30 14964.0 31 19413.0 32 26139.0 33 35819.0 34 64745.0 35 101618.0 36 198915.0 37 337883.0 38 47180.0 39 291.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 73.24700578121795 9.164758432163833 5.897700490210443 11.690535296407775 2 10.61393815707843 32.17289949010818 45.74237198916297 11.470790363650416 3 39.3453580791043 19.84020387061736 18.391803398539903 22.422634651738434 4 15.502732977580905 15.626441126737586 48.47268015848864 20.39814573719286 5 16.914229952284384 31.964761280872278 28.611190341113897 22.509818425729446 6 30.251729583396 38.41690897545161 15.353603716515432 15.977757724636954 7 46.82371249229741 20.87825206011677 17.394591997660502 14.903443449925325 8 45.27838992344408 20.601063218690925 16.731176955935958 17.38936990192904 9 56.84501864288176 8.552539505154208 13.835107105183454 20.767334746780577 10-11 37.53459638422091 24.78746070372962 22.96745589940155 14.710487012647915 12-13 28.50977054111356 27.943643142866094 27.00465810939246 16.541928206627883 14-15 24.318516507044606 27.40341733944667 26.160558555359437 22.117507598149288 16-17 21.93687530679812 33.31979069840308 24.959215432337306 19.784118562461487 18-19 22.546554983445958 24.997441173091588 28.569667979153397 23.886335864309064 20-21 22.467544675028982 26.155963111115753 26.531118468463767 24.8453737453915 22-23 23.662307957429476 23.038058633690873 26.419261177895915 26.88037223098374 24-25 19.779000908644655 21.446886064315333 38.6290954285774 20.145017598462616 26-27 16.566837603266944 21.925491138103546 35.59761663550816 25.910054623121354 28-29 20.805508266577544 22.53020982380649 26.64689233083021 30.017389578785757 30-31 20.672031499681452 27.120223087929645 25.755167263726282 26.45257814866262 32-33 29.74965273063386 22.53830407219025 23.37493602932729 24.3371071678486 34-35 27.335164548236495 17.050664772786615 30.60518867431878 25.008982004658108 36-37 24.105768326944972 24.71288917668439 31.89729182115367 19.284050675216978 38-39 22.15860549155587 29.235328522042465 26.381139879056263 22.2249261073454 40-41 19.88699384837123 27.777893824349587 28.653378173728683 23.6817341535505 42-43 21.207035207369422 21.75525081725798 25.254524945951307 31.783189029421287 44-45 22.830845875066583 22.92285920185489 24.531003582357673 29.715291340720857 46-47 24.21726007081162 21.629398310129822 24.702027217562954 29.45131440149561 48-49 17.281951392732932 22.109935559338673 26.854000647539873 33.75411240038852 50-51 23.099888247151345 23.585229824433142 26.518846543494835 26.796035384920675 52-53 24.498626588822624 21.00890889531787 29.984281491848307 24.508183024011196 54-55 20.915224497895498 23.50616729505885 29.49444891223746 26.08415929480819 56-57 20.719970338496246 25.941282755595473 27.68692491670757 25.651821989200705 58-59 23.39065453747898 22.02037661754415 32.020742164245355 22.568226680731513 60-61 25.061986276332416 28.74669702444985 27.80040105695218 18.390915642265554 62-63 26.10812871421559 23.601209437371406 26.986694100076242 23.303967748336763 64-65 30.63709567923799 18.547839618995894 29.056680627069255 21.758384074696856 66-67 30.228048920592816 23.61901678381568 24.243057223725025 21.909877071866482 68-69 22.35547850063187 28.52763010851515 25.406696815566022 23.710194575286955 70-71 24.331885072117142 27.26978390967863 23.028919966160817 25.369411052043407 72-73 22.858105214784796 28.161770081569138 24.19292510470302 24.787199598943047 74-75 20.3615256874889 27.535014151879434 26.906430488683718 25.197029671947945 76-77 22.41746477696429 28.699645941909406 22.871369337942703 26.011519943183597 78-79 22.658151169227235 29.151879432253754 19.76688564654767 28.42308375197134 80-81 21.647988970933817 22.286442395061986 25.18569772421068 30.879870909793517 82-83 24.266817759303162 18.138113987905626 28.662046852642902 28.933021400148306 84-85 25.81480359697954 19.833571809038403 31.437904059657225 22.913720534324835 86-87 27.53893072367803 23.074143315195254 24.076002381275654 25.310923579851064 88-89 22.137142678099575 25.242984114384786 27.19228800902378 25.427585198491858 90-91 19.22060221207975 26.700209928248402 26.524695290714067 27.554492568957773 92-93 23.972030455262306 22.308740743835315 26.836976615455317 26.882252185447065 94-95 21.546366987999622 21.66501300301837 29.095689682183252 27.69293032679875 96-97 21.664856340146425 20.383249605731773 28.28710037912415 29.664793674997654 98-99 19.449121121288396 22.739406978808734 29.877750738926544 27.933721160976322 100-101 19.18655414791064 26.63441152203202 27.722174062894922 26.45686026716242 102-103 20.81219254911381 26.013452118604235 26.422655540121358 26.751699792160586 104-105 19.754143732962913 22.906096274556905 25.174156892644156 32.16560309983603 106-107 19.605940656104107 25.31672010611298 23.303079992062415 31.774259245720494 108-109 21.95332490835222 24.834616228184693 25.683572331247976 27.52848653221511 110-111 23.816138533202434 27.315135880350095 22.21387838656445 26.654847199883026 112-113 20.090091877840177 27.3685672717702 24.124956787022427 28.416384063367197 114-115 20.876128765423587 23.862513603673044 25.294058667602474 29.96729896330089 116-117 24.60211893373358 23.665012734805316 23.77191511940218 27.960953212058925 118-119 21.44489459990997 25.095801647506338 25.206042736569227 28.253261016014463 120-121 19.849080645245518 24.551289081063015 26.925422861410077 28.674207412281387 122-123 19.569513217908803 26.16050325290798 28.54381059709583 25.726172932087387 124-125 19.72748784220726 27.11608276179084 23.89963487445218 29.256794521549722 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 2.5 2 37.0 3 119.5 4 190.0 5 247.0 6 319.0 7 408.5 8 533.0 9 657.5 10 778.0 11 893.0 12 1003.0 13 1108.0 14 1200.0 15 1299.5 16 1422.5 17 1513.5 18 1569.5 19 1643.5 20 1741.5 21 1912.0 22 1999.0 23 2125.0 24 2313.0 25 2471.5 26 2669.5 27 2801.5 28 2908.5 29 3072.5 30 3392.5 31 3722.5 32 4078.0 33 4596.0 34 5223.5 35 5616.0 36 6031.0 37 6794.5 38 7301.5 39 7647.5 40 7974.0 41 8510.0 42 9074.5 43 9706.5 44 10664.0 45 13692.0 46 27417.0 47 56687.5 48 83179.5 49 99283.0 50 100132.0 51 84931.5 52 67345.0 53 51481.0 54 39387.5 55 31605.5 56 26929.5 57 22938.0 58 19396.0 59 15261.0 60 12739.0 61 11283.5 62 8974.5 63 6623.0 64 5157.5 65 4765.0 66 4348.0 67 3883.0 68 3641.5 69 3435.0 70 2969.0 71 2180.0 72 1714.0 73 1454.0 74 1081.5 75 870.5 76 667.5 77 532.0 78 533.5 79 434.0 80 379.5 81 370.0 82 242.5 83 106.0 84 41.5 85 36.0 86 32.0 87 14.5 88 3.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20491503650244916 2 0.0012533029755501478 3 0.0 4 0.12397255266483546 5 0.03561469288855003 6 0.0014621868048085057 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0010444191462917899 112-113 3.1332574388753695E-4 114-115 8.355353170334318E-4 116-117 0.005692084347290254 118-119 0.0024021640364711165 120-121 5.222095731458949E-4 122-123 0.0019843963779544007 124-125 0.027677107376732433 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 957470.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.4250078731611 #Duplication Level Percentage of deduplicated Percentage of total 1 82.14545769218998 31.56439858566821 2 8.973733727473544 6.896315782596445 3 2.7571559724456347 3.1783121984627 4 1.3571970557922275 2.086012302169896 5 0.8456530657966088 1.6247112855598755 6 0.5554171755156575 1.2805145605246824 7 0.4321230837341177 1.1623033026260685 8 0.3331799635863715 1.0241954179188681 9 0.2587029375935662 0.8946596172458419 >10 1.9462969572323456 14.866853405569847 >50 0.20993014041061075 5.639795355488049 >100 0.15928537281501431 12.381698931218361 >500 0.014703265182897685 3.7712999364080972 >1k 0.009257611411454097 6.664799105284043 >5k 8.168480657165379E-4 2.139783229745836 >10k+ 0.0010891307542887172 4.8243469835131165 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 12616 1.317639194961722 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 11364 1.1868779178459898 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 11077 1.1569030883474156 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 11054 1.1545009243109443 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 8475 0.8851452264822918 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 6132 0.6404378205061255 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 5845 0.6104629910075512 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 3819 0.39886367196883454 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 3742 0.39082164454238777 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 3623 0.37839305670151546 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 3545 0.37024658736043947 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 3331 0.3478960176297952 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 2303 0.2405297293909992 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 2267 0.23676982046434875 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 2139 0.22340125539181385 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 2135 0.22298348773329715 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 2035 0.21253929627037924 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 1959 0.20460171075856165 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1916 0.20011070842950696 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 1914 0.19990182460024858 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 1833 0.19144202951528508 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 1829 0.19102426185676835 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 1765 0.1843399793205009 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1674 0.17483576508924561 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 1643 0.17159806573574107 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 1525 0.15927391980949795 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 1520 0.15875171023635204 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 1513 0.1580206168339478 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 1386 0.14475649367604207 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 1343 0.14026549134698738 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1336 0.1395343979445831 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1238 0.12929909031092357 No Hit ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA 1228 0.1282546711646318 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1221 0.12752357776222753 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1176 0.12282369160391449 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1173 0.12251036586002695 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1167 0.12188371437225187 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 1166 0.1217792724576227 No Hit CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 1148 0.11989931799429748 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 1083 0.11311059354340083 No Hit ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA 1007 0.10517300803158323 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.222095731458949E-5 0.0 12-13 0.0 0.0 0.0 1.0444191462917898E-4 0.0 14-15 0.0 1.0444191462917898E-4 0.0 1.0444191462917898E-4 0.0 16-17 0.0 1.0444191462917898E-4 0.0 1.0444191462917898E-4 0.0 18-19 0.0 1.0444191462917898E-4 0.0 2.0888382925835796E-4 0.0 20-21 0.0 1.0444191462917898E-4 0.0 8.877562743480214E-4 0.0 22-23 0.0 1.0444191462917898E-4 0.0 0.002872152652302422 0.0 24-25 0.0 1.0444191462917898E-4 0.0 0.004595444243683876 0.0 26-27 0.0 1.0444191462917898E-4 0.0 0.007519817853300886 0.0 28-29 0.0 1.0444191462917898E-4 0.0 0.014099658474939162 0.0 30-31 0.0 1.0444191462917898E-4 0.0 0.04256008021139043 0.0 32-33 0.0 1.0444191462917898E-4 0.0 0.10172642484882033 0.0 34-35 0.0 1.0444191462917898E-4 0.0 0.17217249626620154 0.0 36-37 0.0 1.0444191462917898E-4 0.0 0.2569793309450949 0.0 38-39 0.0 1.0444191462917898E-4 0.0 0.3949993211275549 0.0 40-41 0.0 1.0444191462917898E-4 0.0 0.6160506334402123 0.0 42-43 0.0 1.0444191462917898E-4 0.0 1.1315759240498395 0.0 44-45 0.0 1.0444191462917898E-4 0.0 1.8597449528444754 0.0 46-47 0.0 1.0444191462917898E-4 0.0 2.6021703029859946 0.0 48-49 0.0 1.0444191462917898E-4 0.0 2.8698027092232654 0.0 50-51 0.0 1.0444191462917898E-4 0.0 3.2061578952865366 0.0 52-53 0.0 1.0444191462917898E-4 0.0 3.477289105663885 0.0 54-55 0.0 1.0444191462917898E-4 0.0 3.7574545416566574 0.0 56-57 0.0 1.0444191462917898E-4 0.0 4.211411323592384 0.0 58-59 0.0 1.0444191462917898E-4 0.0 4.730122092598202 0.0 60-61 0.0 1.0444191462917898E-4 0.0 5.467429788922891 0.0 62-63 0.0 1.0444191462917898E-4 0.0 6.357953774008585 0.0 64-65 0.0 1.0444191462917898E-4 0.0 7.180120525969482 0.0 66-67 0.0 1.0444191462917898E-4 0.0 7.849854303529092 0.0 68-69 0.0 1.0444191462917898E-4 0.0 8.381306986119668 0.0 70-71 0.0 1.0444191462917898E-4 0.0 8.986965649054278 0.0 72-73 0.0 1.0444191462917898E-4 0.0 9.553824140704148 0.0 74-75 0.0 1.0444191462917898E-4 0.0 10.193844193551756 0.0 76-77 0.0 1.0444191462917898E-4 0.0 11.00948332584833 0.0 78-79 0.0 1.0444191462917898E-4 0.0 12.038862836433518 0.0 80-81 0.0 1.0444191462917898E-4 0.0 14.364888717139962 0.0 82-83 0.0 1.0444191462917898E-4 0.0 15.959455648740953 0.0 84-85 0.0 1.0444191462917898E-4 0.0 16.659947570158856 0.0 86-87 0.0 1.0444191462917898E-4 0.0 17.253908738655 0.0 88-89 0.0 1.0444191462917898E-4 0.0 17.944635341055072 0.0 90-91 0.0 1.0444191462917898E-4 0.0 18.84215693442092 0.0 92-93 0.0 1.0444191462917898E-4 0.0 20.02047061526732 0.0 94-95 0.0 1.0444191462917898E-4 0.0 21.574044095376358 0.0 96-97 0.0 1.0444191462917898E-4 0.0 23.300730048983258 0.0 98-99 0.0 1.0444191462917898E-4 0.0 25.009817539975145 0.0 100-101 0.0 1.0444191462917898E-4 0.0 26.482344094331935 0.0 102-103 0.0 1.0444191462917898E-4 0.0 27.708126625377297 0.0 104-105 0.0 1.0444191462917898E-4 0.0 28.950202095104807 0.0 106-107 0.0 1.0444191462917898E-4 0.0 30.17514909083313 0.0 108-109 0.0 1.0444191462917898E-4 0.0 31.177478145529363 0.0 110-111 0.0 1.0444191462917898E-4 0.0 32.236153613168035 0.0 112-113 0.0 1.0444191462917898E-4 0.0 35.52356731803607 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTAGTA 1575 0.0 96.884636 2 GAGTACG 390 0.0 96.47461 1 GAGTAGT 1925 0.0 95.86605 1 GAGTCCG 590 0.0 93.12641 1 AGTCGCG 45 4.0017767E-10 92.72163 2 AGTTCGT 85 0.0 91.16328 2 AGTCGTA 290 0.0 90.43784 2 AGTCCGT 245 0.0 87.58545 2 GAGTAAG 915 0.0 87.46227 1 GAGTAAT 405 0.0 87.00296 1 GAGTCGA 400 0.0 86.597435 1 AGTACGG 255 0.0 86.488235 2 GAGTCGG 685 0.0 86.31409 1 GAGTTAG 1050 0.0 85.31767 1 GAGTCAG 4570 0.0 82.853355 1 GAGTCGT 645 0.0 81.48168 1 GAGTAGC 555 0.0 78.55376 1 GAGTTAC 540 0.0 78.523865 1 GAGTTCG 415 0.0 77.71104 1 TCGTAGG 1090 0.0 77.65285 2 >>END_MODULE