##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935911_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1575858 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53492510111952 33.0 31.0 34.0 31.0 34.0 2 32.63370367126987 34.0 31.0 34.0 31.0 34.0 3 32.810408044379635 34.0 31.0 34.0 31.0 34.0 4 36.05872293061938 37.0 35.0 37.0 35.0 37.0 5 36.09385363402033 37.0 35.0 37.0 35.0 37.0 6 36.20606044453244 37.0 37.0 37.0 35.0 37.0 7 36.23823402870055 37.0 37.0 37.0 35.0 37.0 8 36.23637472411854 37.0 37.0 37.0 35.0 37.0 9 38.05341598037386 39.0 39.0 39.0 35.0 39.0 10-11 37.77015600390391 39.0 38.0 39.0 35.0 39.0 12-13 37.67909100946913 39.0 37.5 39.0 35.0 39.0 14-15 39.21091176996912 41.0 39.0 41.0 35.5 41.0 16-17 39.263539608264196 41.0 39.0 41.0 35.5 41.0 18-19 39.259590648395985 41.0 39.0 41.0 35.0 41.0 20-21 39.284154727139125 41.0 39.0 41.0 35.0 41.0 22-23 39.16883405738334 41.0 39.0 41.0 35.0 41.0 24-25 38.99642670849785 41.0 38.0 41.0 35.0 41.0 26-27 38.89687617792974 41.0 38.0 41.0 35.0 41.0 28-29 38.78625739121164 40.0 38.0 41.0 35.0 41.0 30-31 38.67021711347088 40.0 38.0 41.0 35.0 41.0 32-33 38.52822938361197 40.0 38.0 41.0 35.0 41.0 34-35 38.15616730695278 40.0 37.0 41.0 34.0 41.0 36-37 38.05406134309056 40.0 38.0 41.0 34.0 41.0 38-39 37.78744055619225 40.0 37.5 41.0 33.0 41.0 40-41 37.57526471293733 40.0 37.0 41.0 33.0 41.0 42-43 37.40271521926468 40.0 37.0 41.0 33.0 41.0 44-45 37.22871540456056 40.0 36.0 41.0 33.0 41.0 46-47 37.03547908504447 40.0 36.0 41.0 32.5 41.0 48-49 36.81699524957198 40.0 35.0 41.0 32.0 41.0 50-51 36.55008001990027 39.5 35.0 41.0 31.0 41.0 52-53 36.25778052337203 39.0 35.0 41.0 31.0 41.0 54-55 35.99414414242908 39.0 35.0 41.0 31.0 41.0 56-57 35.73497897653215 38.0 35.0 41.0 31.0 41.0 58-59 35.38871459230464 37.0 35.0 40.0 30.0 41.0 60-61 35.07211309648458 37.0 35.0 39.0 30.0 41.0 62-63 34.64318104803859 36.0 35.0 39.0 29.5 41.0 64-65 34.20637265540423 35.5 34.5 38.5 29.0 41.0 66-67 33.773760706865716 35.0 34.0 37.0 29.0 39.5 68-69 33.423979191018475 35.0 34.0 37.0 29.0 39.0 70-71 33.24864740350971 35.0 34.0 36.0 29.0 39.0 72-73 32.9569618582385 35.0 34.0 35.5 27.5 37.5 74-75 32.659042566018 35.0 34.0 35.0 27.0 37.0 76-77 32.53127407418688 35.0 34.0 35.0 27.0 36.5 78-79 32.57113648564781 35.0 34.0 35.0 28.5 36.0 80-81 32.52681364691489 35.0 34.0 35.0 29.0 36.0 82-83 32.453610985253746 35.0 34.0 35.0 29.0 35.5 84-85 32.273272401447336 35.0 34.0 35.0 27.5 35.0 86-87 32.176459109894424 35.0 33.5 35.0 27.0 35.0 88-89 32.13562421233385 35.0 34.0 35.0 27.0 35.0 90-91 32.14291738214992 35.0 34.0 35.0 27.0 35.0 92-93 32.14660584900416 35.0 34.0 35.0 27.0 36.0 94-95 32.063965154220746 35.0 34.0 35.0 27.0 35.0 96-97 32.032223715588586 35.0 34.0 35.0 27.0 35.0 98-99 31.95503687514992 35.0 34.0 35.0 27.0 35.0 100-101 31.90658295354023 35.0 34.0 35.0 27.0 35.0 102-103 31.196952390380353 34.5 32.0 35.0 24.5 35.0 104-105 31.791046528303944 35.0 33.0 35.0 26.0 35.0 106-107 31.897811858682697 35.0 34.0 35.0 27.0 35.0 108-109 31.821989988945703 35.0 33.5 35.0 26.0 35.0 110-111 31.723458268448045 35.0 33.0 35.0 25.0 35.0 112-113 31.68127236083454 35.0 33.0 35.0 25.0 35.0 114-115 31.563917561100048 35.0 33.0 35.0 25.0 35.0 116-117 31.41586551580155 35.0 33.0 35.0 25.0 35.0 118-119 31.234949468797318 35.0 33.0 35.0 23.5 35.0 120-121 30.928626817898568 35.0 33.0 35.0 19.0 35.0 122-123 30.561125431352316 35.0 33.0 35.0 2.0 35.0 124-125 28.24275791346682 33.0 29.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09414946025085413 1101 2 0.13885051225438616 1101 3 0.059547786418910675 1101 4 0.1432242455268522 1101 5 0.2334064238277378 1101 6 0.18490924687797872 1101 7 0.17968134620267762 1101 8 0.12458845064782764 1101 9 0.17992304634163503 1101 10-11 0.1597447584525753 1101 12-13 0.22215766309054175 1101 14-15 0.21655421944548436 1101 16-17 0.09903165356764987 1101 18-19 0.21722004748023238 1101 20-21 0.1957998367224718 1101 22-23 0.2670077930222945 1101 24-25 0.2591414567580941 1101 26-27 0.3194871682857823 1101 28-29 0.2893757161740069 1101 30-31 0.2907201740405796 1101 32-33 0.30463571983857207 1101 34-35 0.3863810291010523 1101 36-37 0.2903562762325933 1101 38-39 0.3304201473131201 1101 40-41 0.3230053442233256 1101 42-43 0.24094994782946344 1101 44-45 0.3147678752836285 1101 46-47 0.37830755043895437 1101 48-49 0.4009778157195072 1101 50-51 0.3966376666344402 1101 52-53 0.4143276210963833 1101 54-55 0.438526055548941 1101 56-57 0.25161074507891357 1101 58-59 0.24860877902093392 1101 60-61 0.22393500695911683 1101 62-63 0.32701186189546405 1101 64-65 0.3608598575691744 1101 66-67 0.14645235967086734 1101 68-69 0.2973663834442348 1101 70-71 0.3337075289841067 1101 72-73 0.2634938955442081 1101 74-75 0.30815071284429507 1101 76-77 0.2330864804997006 1101 78-79 0.2706951648514533 1101 80-81 0.32181215010582775 1101 82-83 0.31632496840217783 1101 84-85 0.31095602363211583 1101 86-87 0.19911611030013887 1101 88-89 0.011235052829597691 1101 90-91 0.1384916569980632 1101 92-93 0.1877494458541591 1101 94-95 0.16537208562542816 1101 96-97 0.28297118108457653 1101 98-99 0.16393156300617306 1101 100-101 0.05982321962937576 1101 102-103 0.02662562300178095 1101 104-105 0.07239266917883569 1101 106-107 0.02757611493360912 1101 108-109 0.06668587411751759 1101 110-111 0.049325489965049485 1101 112-113 0.11784413734332233 1101 114-115 0.11613522864826464 1101 116-117 0.048640603487363876 1101 118-119 -0.23759983765368986 1101 120-121 -0.25491371181446 1101 122-123 -0.12080631416107224 1101 124-125 -0.026859836411563975 1102 1 0.06907769121175278 1102 2 0.02986482543822433 1102 3 0.15259533002198822 1102 4 0.16535416102896505 1102 5 0.1330694140238151 1102 6 0.1850869768097354 1102 7 0.21381988076014835 1102 8 0.15929318198787712 1102 9 0.22495811838150104 1102 10-11 0.19361966027794608 1102 12-13 0.2076049670121094 1102 14-15 0.27823200692787964 1102 16-17 0.2637483689831015 1102 18-19 0.1751611054642055 1102 20-21 0.31252143040137526 1102 22-23 0.29005851883748335 1102 24-25 0.25470370962885625 1102 26-27 0.26341802964967087 1102 28-29 0.22022015339641143 1102 30-31 0.29944798318910415 1102 32-33 0.3907991907518067 1102 34-35 0.4190905303739356 1102 36-37 0.3618147111258807 1102 38-39 0.32232412575532265 1102 40-41 0.27231507502173 1102 42-43 0.22541762129387877 1102 44-45 0.2293606029133528 1102 46-47 0.2770438059908926 1102 48-49 0.43243855263857967 1102 50-51 0.42470122285802603 1102 52-53 0.5060798520301901 1102 54-55 0.4257019946400433 1102 56-57 0.3597632170483607 1102 58-59 0.2636807003999806 1102 60-61 0.3386601010713477 1102 62-63 0.35708292001484665 1102 64-65 0.4445351094378722 1102 66-67 0.4091782459507911 1102 68-69 0.3674482576994649 1102 70-71 0.2930598472594781 1102 72-73 0.25267902919711105 1102 74-75 0.375941563645938 1102 76-77 0.3396131467601222 1102 78-79 0.3345755339880654 1102 80-81 0.3700628644108903 1102 82-83 0.3630975621081731 1102 84-85 0.3337029158973053 1102 86-87 0.3711506440722161 1102 88-89 0.34271153154263345 1102 90-91 0.31875677927625645 1102 92-93 0.295309907884274 1102 94-95 0.35124204825135763 1102 96-97 0.29702623335410294 1102 98-99 0.36125283440146916 1102 100-101 0.29650363074409825 1102 102-103 0.3563586557176279 1102 104-105 0.4101222793239998 1102 106-107 0.522369051099794 1102 108-109 0.4999563249083252 1102 110-111 0.5147663279024357 1102 112-113 0.30842225049466165 1102 114-115 0.27615733486644345 1102 116-117 0.4360685805483584 1102 118-119 0.4052848714785817 1102 120-121 0.5650213236638209 1102 122-123 0.6306210264574936 1102 124-125 0.4752145257918805 1103 1 0.12503592859923174 1103 2 0.14690022945748638 1103 3 0.17607698691961815 1103 4 0.250403616754312 1103 5 0.18032220081468608 1103 6 0.20997511266905633 1103 7 0.21456727713862733 1103 8 0.20814379429685914 1103 9 0.259073410405783 1103 10-11 0.2743016717391171 1103 12-13 0.1913895729363304 1103 14-15 0.2167795066662066 1103 16-17 0.2434034872581634 1103 18-19 0.29222676513554546 1103 20-21 0.22726672687405625 1103 22-23 0.3568695403991313 1103 24-25 0.39171520350576117 1103 26-27 0.3114255723019568 1103 28-29 0.40740614879101145 1103 30-31 0.42855048606747914 1103 32-33 0.37907635215016455 1103 34-35 0.4612660916571656 1103 36-37 0.4326505521348807 1103 38-39 0.40794598570926865 1103 40-41 0.329127012430142 1103 42-43 0.26178246193654076 1103 44-45 0.4025889300538168 1103 46-47 0.37045690521635066 1103 48-49 0.31899948261555977 1103 50-51 0.2795378750455981 1103 52-53 0.27749676510325116 1103 54-55 0.25338460794338147 1103 56-57 0.29629748934269173 1103 58-59 0.2677962868035806 1103 60-61 0.20689510080967466 1103 62-63 0.2468329592654257 1103 64-65 0.3261488314391343 1103 66-67 0.37605157780132714 1103 68-69 0.23173693914178983 1103 70-71 0.2694113754152241 1103 72-73 0.2743347166055301 1103 74-75 0.19237888701835004 1103 76-77 0.15868189414342027 1103 78-79 0.20800387552039723 1103 80-81 0.29617937974477826 1103 82-83 0.37026896937214815 1103 84-85 0.31337929490086935 1103 86-87 0.408900702948074 1103 88-89 0.35000886773689643 1103 90-91 0.3192662190397044 1103 92-93 0.3741283347212061 1103 94-95 0.33668335555719864 1103 96-97 0.39377726695259696 1103 98-99 0.32965693870333723 1103 100-101 0.34844195500618014 1103 102-103 0.35935805387546793 1103 104-105 0.27061019668845887 1103 106-107 0.28768249965536796 1103 108-109 0.26781288367952527 1103 110-111 0.41995983324051167 1103 112-113 0.38708858135084867 1103 114-115 0.4359546056430794 1103 116-117 0.4716042527798834 1103 118-119 0.43874598724161373 1103 120-121 0.39539505456187385 1103 122-123 0.17679687557828316 1103 124-125 0.23193128055428147 1104 1 0.12713842094078842 1104 2 0.13924062501860135 1104 3 0.13913399453085162 1104 4 0.17722233261251574 1104 5 0.13800262507465533 1104 6 0.15436509965086742 1104 7 0.1112007352876958 1104 8 0.08062606649009041 1104 9 0.11343024820777003 1104 10-11 0.22661044671322372 1104 12-13 0.17347707732862006 1104 14-15 0.3105929239186409 1104 16-17 0.3308491544908634 1104 18-19 0.2854483267304815 1104 20-21 0.1990888598144238 1104 22-23 0.32682882178249884 1104 24-25 0.2749221989656476 1104 26-27 0.3525647454532148 1104 28-29 0.29489136594406773 1104 30-31 0.2836461759775304 1104 32-33 0.35100887271070746 1104 34-35 0.34884689423417825 1104 36-37 0.22322724182976827 1104 38-39 0.22642365432272982 1104 40-41 0.12173769802917178 1104 42-43 0.0750746213002742 1104 44-45 0.1997210577466504 1104 46-47 0.13766615138403182 1104 48-49 0.07776528516934889 1104 50-51 0.10655718484917287 1104 52-53 0.16339379367362028 1104 54-55 0.2786788523851058 1104 56-57 0.28419577484203273 1104 58-59 0.19452609273808008 1104 60-61 0.11535619096336802 1104 62-63 0.10949305517591057 1104 64-65 0.1701599015469739 1104 66-67 0.2828512423622769 1104 68-69 0.31310867511240303 1104 70-71 0.2515992058041121 1104 72-73 0.29120051312234807 1104 74-75 0.23329612397267852 1104 76-77 0.264388924432005 1104 78-79 0.25111793253730497 1104 80-81 0.22727989691222916 1104 82-83 0.23723621414358576 1104 84-85 0.2998804659190597 1104 86-87 0.1895562448129482 1104 88-89 0.25266478807360926 1104 90-91 0.3090143920810533 1104 92-93 0.24190873282094572 1104 94-95 0.24704193247212203 1104 96-97 0.20577911983927777 1104 98-99 0.24876496803373982 1104 100-101 0.28210518704734966 1104 102-103 0.21223709071494312 1104 104-105 0.2534228152027751 1104 106-107 0.2358751031236892 1104 108-109 0.2895714348986047 1104 110-111 0.3013379351229659 1104 112-113 0.2666743597877037 1104 114-115 0.1380392192535176 1104 116-117 0.20800296938109852 1104 118-119 0.3095375196887993 1104 120-121 0.23961509685165083 1104 122-123 0.3767413625051397 1104 124-125 0.33403121879139164 1105 1 0.09056440683428235 1105 2 0.15861261031290042 1105 3 0.16874223904567742 1105 4 0.15339806194433692 1105 5 0.21780174242331185 1105 6 0.15957439043577892 1105 7 0.15243122825753375 1105 8 0.13931123764967168 1105 9 0.14053236173293726 1105 10-11 0.21659252180552357 1105 12-13 0.13297257423054987 1105 14-15 0.28184104766705786 1105 16-17 0.2354840073216593 1105 18-19 0.17964583783244592 1105 20-21 0.18107675036746684 1105 22-23 0.2626061041915193 1105 24-25 0.2987889863760955 1105 26-27 0.2924097771126313 1105 28-29 0.3035566739604292 1105 30-31 0.2524541592453531 1105 32-33 0.2403077197811001 1105 34-35 0.2359955819752031 1105 36-37 0.24231009442224405 1105 38-39 0.23686929200127338 1105 40-41 0.16617033331489495 1105 42-43 0.21583331983922704 1105 44-45 0.2438163872589456 1105 46-47 0.3586134055472314 1105 48-49 0.34087877772081043 1105 50-51 0.28165611876307395 1105 52-53 0.28836699681333045 1105 54-55 0.35820571174782856 1105 56-57 0.42980430443585504 1105 58-59 0.415603536748435 1105 60-61 0.34602469555998283 1105 62-63 0.38110527684444406 1105 64-65 0.4541565620501231 1105 66-67 0.42103583968724223 1105 68-69 0.4671487177762117 1105 70-71 0.4086248476419456 1105 72-73 0.5002217739445527 1105 74-75 0.49342816532497125 1105 76-77 0.4585170700817329 1105 78-79 0.4293424597765778 1105 80-81 0.3942687858074052 1105 82-83 0.3227095725495417 1105 84-85 0.3575671064044599 1105 86-87 0.4135574454631197 1105 88-89 0.3649986404694303 1105 90-91 0.3593610025162661 1105 92-93 0.4079988309827769 1105 94-95 0.37849087853770413 1105 96-97 0.4239347869250736 1105 98-99 0.3708124348183617 1105 100-101 0.3652239098180132 1105 102-103 0.36980677716386623 1105 104-105 0.4302112946928318 1105 106-107 0.4435788162965011 1105 108-109 0.5073043856378234 1105 110-111 0.48971349955392895 1105 112-113 0.3955019742892567 1105 114-115 0.4569559831619152 1105 116-117 0.4508775498935691 1105 118-119 0.6021067244070082 1105 120-121 0.4389653542994836 1105 122-123 0.4407775154997964 1105 124-125 0.5174471251059849 1106 1 0.06336321081133178 1106 2 0.10632348914801781 1106 3 0.13528850733503361 1106 4 0.15262279426153214 1106 5 0.13090712587771947 1106 6 0.15143426235410828 1106 7 0.16469199719307426 1106 8 0.07746693316484254 1106 9 0.09791546265029183 1106 10-11 0.10801569854792348 1106 12-13 0.1513208195072835 1106 14-15 0.12352503278395943 1106 16-17 0.13837050090242542 1106 18-19 0.2298472504044895 1106 20-21 0.15885901371818534 1106 22-23 0.1604358294078736 1106 24-25 0.16945941946333676 1106 26-27 0.20161627256309345 1106 28-29 0.1672752601906069 1106 30-31 0.14311338874686186 1106 32-33 0.22221427574114472 1106 34-35 0.18909024387559725 1106 36-37 0.09066145464637287 1106 38-39 0.12416251591341165 1106 40-41 0.0770060594609916 1106 42-43 -0.006024061581882734 1106 44-45 -0.08349848791126391 1106 46-47 -0.09090910916842887 1106 48-49 -0.019869927092820205 1106 50-51 -0.06720055312194972 1106 52-53 0.07532979163324427 1106 54-55 0.039743239560515065 1106 56-57 0.04158919485945489 1106 58-59 0.010602585466138237 1106 60-61 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38-39 -1.0356592919707452 2216 40-41 -0.941094915042676 2216 42-43 -0.85749608639005 2216 44-45 -0.7152152375724157 2216 46-47 -0.8778202229540213 2216 48-49 -1.0663410072623023 2216 50-51 -1.029197190139648 2216 52-53 -1.0660790195312515 2216 54-55 -1.305569346695627 2216 56-57 -1.1655303806257464 2216 58-59 -0.8702859337964384 2216 60-61 -0.8007989811726617 2216 62-63 -0.8059381377672352 2216 64-65 -0.8896952707483905 2216 66-67 -1.072454270837973 2216 68-69 -0.9724265410448254 2216 70-71 -1.015505566085345 2216 72-73 -0.7933281958082787 2216 74-75 -0.8736081360416321 2216 76-77 -1.0871640945851304 2216 78-79 -1.0150649365163815 2216 80-81 -0.9890687022114868 2216 82-83 -1.0056640718189023 2216 84-85 -1.1407419101100231 2216 86-87 -1.2061610097559878 2216 88-89 -1.1152959770163449 2216 90-91 -1.0220986734817643 2216 92-93 -1.3062273416309154 2216 94-95 -1.3910365405345821 2216 96-97 -1.2330665462791188 2216 98-99 -1.5138503185049927 2216 100-101 -1.3627730607017128 2216 102-103 -1.4891723451442545 2216 104-105 -1.3736904578435158 2216 106-107 -1.4744296550279614 2216 108-109 -1.343722880213889 2216 110-111 -1.2738541950930866 2216 112-113 -1.1970802977468544 2216 114-115 -1.3427262495502283 2216 116-117 -1.3505927853869082 2216 118-119 -1.2308455422253637 2216 120-121 -1.3609463123221985 2216 122-123 -1.3112642345913663 2216 124-125 -1.6238341481648852 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 25.0 4 72.0 5 162.0 6 403.0 7 840.0 8 1206.0 9 1993.0 10 4490.0 11 14242.0 12 20033.0 13 6676.0 14 5918.0 15 7587.0 16 4695.0 17 3053.0 18 2591.0 19 2605.0 20 2577.0 21 2871.0 22 3184.0 23 3619.0 24 4916.0 25 6521.0 26 8566.0 27 11298.0 28 14950.0 29 19445.0 30 24964.0 31 33018.0 32 44123.0 33 61080.0 34 108813.0 35 171171.0 36 336932.0 37 567732.0 38 73222.0 39 261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 69.56092362028322 10.49275985325754 6.703757143647487 13.24255938281175 2 11.9184718323087 29.969387910509138 44.67353450332773 13.438605753854432 3 37.27588399462388 19.69212962081609 19.016624594347967 24.01536179021206 4 15.640817934325096 15.798635929007315 46.162589384134975 22.397956752532615 5 17.291281962086988 32.68291413362042 28.370329509192597 21.65547439509999 6 31.290673242709673 38.63579683225443 14.816974770611639 15.256555154424264 7 45.332130179242036 22.284622091584396 16.79389894267123 15.589348786502338 8 43.91912215440731 21.852984215582875 15.928592550851665 18.29930107915815 9 53.77362681155282 9.556127519103878 13.29872361596032 23.371522053382982 10-11 37.122443773487205 24.95729310635857 21.73520710622404 16.185056013930186 12-13 29.31015992557705 27.823763308622983 25.296663785696428 17.56941298010354 14-15 25.051813044068695 27.44117807568956 24.50963221305473 22.99737666718702 16-17 22.88496171609371 32.98225474630328 23.3484869829642 20.784296554638807 18-19 22.945627080612592 25.090775945548394 27.46976567685667 24.493831296982343 20-21 23.257203377461675 25.916802148416927 25.371924373896633 25.45407010022477 22-23 24.670116216055 22.81744294219403 25.005838089472526 27.506602752278443 24-25 20.358782326834017 22.61282425193133 36.28496349290355 20.743429928331107 26-27 17.880862361964088 22.257557470279682 34.03691830101443 25.8246618667418 28-29 21.911682397779494 22.726952555369838 26.051300307515017 29.31006473933565 30-31 21.666292267450494 26.376964168091288 25.301105810295088 26.65563775416313 32-33 29.778222403287607 22.656641651722424 22.602512409113004 24.96262353587696 34-35 27.36172929286776 17.73116613298914 29.382438011546725 25.524666562596376 36-37 24.5037941235822 25.024653236522582 30.08653063918196 20.385022000713263 38-39 22.275738042387065 28.868464036734277 26.005483996654522 22.850313924224135 40-41 20.442324118036016 27.627013347649342 28.0559225513974 23.87473998291724 42-43 21.60610283413861 21.495972352838898 24.563190338215755 32.334734474806744 44-45 23.1837830565952 23.303336975793503 23.986710731550687 29.52616923606061 46-47 24.5032230061338 21.468114512855855 24.683696119828056 29.34496636118229 48-49 17.991754333194994 21.89074142467151 27.081913471899117 33.03559077023437 50-51 23.663172697032348 23.376154450464444 26.783060402650495 26.177612449852717 52-53 24.559985734755287 21.02962322747354 29.679450813461617 24.73094022430955 54-55 21.719628291381586 23.361241939311792 28.37308310774194 26.54604666156468 56-57 21.010268691722224 25.611095669787503 27.055388239295674 26.3232473991946 58-59 22.86998574744679 22.24286706035696 31.354728662100268 23.532418530095985 60-61 25.419803053320795 27.774837580543426 27.576945384672985 19.228413981462797 62-63 26.24421109008553 23.54771178621424 26.40396533190173 23.804111791798498 64-65 30.125588726903057 19.124787893325415 28.421945378327234 22.327678001444294 66-67 29.69623532069514 23.858431406890723 24.141420102572695 22.303913169841444 68-69 22.863544811778727 28.715880491770196 24.995240687930004 23.425334008521073 70-71 24.48009274947362 27.17709971329904 23.1019546177384 25.240852919488937 72-73 22.732124341152566 27.95772207901981 24.121811736844307 25.188341842983313 74-75 21.141689162348385 27.47249434911014 26.13671409479788 25.2491023937436 76-77 22.862370848134795 28.291762328839276 22.84085875757841 26.00500806544752 78-79 23.113186594223592 28.663147314034642 20.3780099475968 27.84565614414497 80-81 22.061442084248707 22.273675673823405 25.741437363011133 29.923444878916754 82-83 24.61198915130678 18.078246897880394 28.466682911785202 28.84308103902763 84-85 25.708725024716696 20.530561763813747 30.873276653099452 22.887436558370105 86-87 27.329588072021714 23.04382755299018 24.31913281526635 25.30745155972175 88-89 21.944204363591137 24.55979536227249 27.8876967340966 25.608303540039774 90-91 19.558583324131998 26.13880819210868 26.98660031551066 27.31600816824866 92-93 23.922904221065604 22.18090081720561 26.987203161706198 26.90899180002259 94-95 22.07660842537843 21.93849318910714 28.59426420400823 27.3906341815062 96-97 21.620951887796995 20.039051741971676 28.806783352307125 29.533213017924204 98-99 19.564040668638928 23.172138606397276 29.60564974762955 27.65817097733425 100-101 19.428685833368235 26.835730122891782 27.147623707212198 26.587960336527782 102-103 20.964039907148994 25.463747368100424 26.836015681615983 26.736197043134595 104-105 20.497595595542236 22.440568883744604 25.93783830776631 31.123997212946854 106-107 20.003496515699894 25.055803057959984 23.929874236485666 31.010826189854455 108-109 22.39662456896497 24.617573410802244 26.09489560607618 26.890906414156607 110-111 24.23439196516903 26.6553245604405 22.79975936628044 26.310524108110027 112-113 20.289258663228114 27.048632616178125 24.964558944472085 27.697549776121676 114-115 21.20153137471531 23.71616008995843 26.432173591337694 28.65013494398857 116-117 24.95651318565903 22.966305254907933 24.26297703020438 27.814204529228657 118-119 21.254847485463095 24.936842285620727 25.1644067807366 28.643903448179582 120-121 18.9706097456889 24.469181782097294 27.581415817730864 28.978792654482945 122-123 19.628370906928687 26.05931457207794 28.824385549477938 25.487928971515437 124-125 20.736623245713844 26.7795338492051 24.02720212721727 28.45664077786379 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 3.0 2 26.0 3 73.0 4 149.0 5 231.0 6 318.0 7 423.0 8 562.0 9 723.0 10 861.5 11 1006.0 12 1160.0 13 1290.0 14 1446.5 15 1589.0 16 1679.5 17 1835.5 18 2023.5 19 2130.0 20 2196.0 21 2341.5 22 2479.5 23 2592.5 24 2760.5 25 2976.5 26 3228.5 27 3457.5 28 3665.0 29 3911.5 30 4387.5 31 4916.0 32 5439.5 33 6155.5 34 7087.5 35 7811.0 36 8520.5 37 9589.5 38 10363.0 39 10961.5 40 11629.0 41 12701.0 42 13758.5 43 15066.0 44 17167.5 45 23224.5 46 50351.5 47 100287.5 48 138470.0 49 163289.0 50 167954.0 51 140690.0 52 107984.5 53 81838.5 54 65192.5 55 55195.0 56 46082.5 57 39051.5 58 35079.0 59 30265.0 60 27064.5 61 24018.0 62 17853.5 63 12344.0 64 9236.5 65 8064.0 66 7226.5 67 6246.5 68 5356.5 69 4443.0 70 3527.5 71 2663.5 72 2103.5 73 1808.5 74 1519.0 75 1226.5 76 850.0 77 636.5 78 530.5 79 386.0 80 362.5 81 346.0 82 228.0 83 86.5 84 24.0 85 27.5 86 22.0 87 7.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2098539335396971 2 6.34574942666154E-4 3 0.0 4 0.12012503664670293 5 0.03268060954730693 6 0.0023479272878647694 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 2.2210122993315386E-4 108-109 0.0 110-111 0.0012056923910656926 112-113 1.2691498853323078E-4 114-115 0.0012056923910656926 116-117 0.005901546966795231 118-119 0.002601757264931231 120-121 7.932186783326924E-4 122-123 0.002506571023531308 124-125 0.02960292107537608 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1575858.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.941447508358806 #Duplication Level Percentage of deduplicated Percentage of total 1 78.72686206323527 26.72103656217104 2 10.798272384328428 7.3301799062728765 3 3.400202809804425 3.462234155602531 4 1.6486197574216357 2.238261638310787 5 0.9971116866968844 1.6921706986996707 6 0.6960081375134297 1.417411419888159 7 0.5046741297338827 1.199055933823247 8 0.3761689978292537 1.0214176235274843 9 0.29318831396825873 0.8956112191756087 >10 2.0856715755513324 14.027212562799626 >50 0.24852545983630953 5.852053386645356 >100 0.18855629101298063 12.750492287365113 >500 0.02321844943222894 5.410847973992648 >1k 0.01067299691642782 7.367353352077212 >5k 0.001123473359623981 2.2974718017786913 >10k+ 0.001123473359623981 6.317189477869991 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 20646 1.3101434266285414 No Hit GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 18522 1.1753597088062502 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 17045 1.0816329897744594 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 17000 1.0787774025324617 No Hit GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT 14992 0.951354754045098 No Hit CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT 11194 0.7103431908204927 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 9352 0.5934544863813872 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC 5623 0.3568214902611784 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 5592 0.35485430793891326 No Hit GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG 5346 0.3392437643493259 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 5134 0.3257907755648034 No Hit GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC 5103 0.32382359324253834 No Hit ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC 4978 0.31589140645921143 No Hit GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA 4564 0.28962000383283265 No Hit TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT 3604 0.2287008093368819 No Hit GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC 3452 0.21905527020835633 No Hit CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT 3344 0.2122018608275619 No Hit CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA 3225 0.20465041900983466 No Hit GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC 3166 0.20090642684810434 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA 3129 0.19855849956023955 No Hit GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG 3055 0.19386264498451003 No Hit GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA 2950 0.18719960808651542 No Hit GTGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT 2912 0.18478822330438402 No Hit GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 2838 0.18009236872865447 No Hit AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG 2766 0.1755234291414582 No Hit GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG 2656 0.16854310477213047 No Hit GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2640 0.16752778486386463 No Hit ATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATA 2612 0.1657509750243994 No Hit CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA 2556 0.16219735534546895 No Hit ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA 2527 0.1603570880117371 No Hit GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT 2492 0.15813607571240557 No Hit GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT 2305 0.14626952428454848 No Hit GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC 2194 0.13922574242095417 No Hit GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 2009 0.12748610598163035 No Hit GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG 1988 0.1261534986020314 No Hit GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1977 0.12545546616509864 No Hit GATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACG 1948 0.12361519883136679 No Hit TTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA 1858 0.11790402434737139 No Hit GCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCG 1846 0.11714253441617202 No Hit GGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTG 1826 0.11587338453083972 No Hit GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG 1804 0.11447731965697418 No Hit GACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTT 1748 0.1109236999780437 No Hit GAGTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1737 0.11022566754111095 No Hit ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC 1721 0.1092103476328451 No Hit GAGTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA 1698 0.10775082526471294 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.345749426661539E-5 0.0 10-11 0.0 0.0 0.0 6.345749426661539E-5 0.0 12-13 0.0 0.0 0.0 6.345749426661539E-5 0.0 14-15 0.0 0.0 0.0 9.518624139992309E-5 0.0 16-17 0.0 0.0 0.0 1.2691498853323078E-4 0.0 18-19 0.0 0.0 0.0 1.9037248279984618E-4 0.0 20-21 0.0 0.0 0.0 8.249474254660001E-4 0.0 22-23 0.0 0.0 0.0 0.0019037248279984618 0.0 24-25 6.345749426661539E-5 0.0 0.0 0.0033315184489973084 0.0 26-27 6.345749426661539E-5 0.0 0.0 0.005711174483995385 0.0 28-29 1.9037248279984618E-4 0.0 0.0 0.013484717531655772 0.0 30-31 2.5382997706646155E-4 0.0 0.0 0.043278011089831696 0.0 32-33 2.5382997706646155E-4 0.0 0.0 0.10292805570045016 0.0 34-35 2.5382997706646155E-4 0.0 0.0 0.1780617289121228 0.0 36-37 2.5382997706646155E-4 0.0 0.0 0.27553244010564404 0.0 38-39 5.076599541329231E-4 0.0 0.0 0.42484792411499006 0.0 40-41 5.076599541329231E-4 0.0 0.0 0.6493605388302753 0.0 42-43 5.393887012662309E-4 0.0 0.0 1.1470259376162066 0.0 44-45 5.711174483995385E-4 0.0 0.0 1.8670781250594914 0.0 46-47 6.663036897994616E-4 0.0 0.0 2.615971743646953 0.0 48-49 6.980324369327693E-4 0.0 0.0 2.9160305052866438 0.0 50-51 6.980324369327693E-4 0.0 0.0 3.295918794713737 0.0 52-53 6.980324369327693E-4 0.0 0.0 3.5827466687988387 0.0 54-55 6.980324369327693E-4 0.0 0.0 3.873064705068604 0.0 56-57 6.980324369327693E-4 0.0 0.0 4.313840460244514 0.0 58-59 6.980324369327693E-4 0.0 0.0 4.827719248815566 0.0 60-61 6.980324369327693E-4 0.0 0.0 5.547105132569051 0.0 62-63 6.980324369327693E-4 0.0 0.0 6.404923540065158 0.0 64-65 6.980324369327693E-4 0.0 0.0 7.200490145685715 0.0 66-67 6.980324369327693E-4 0.0 0.0 7.861431677219648 0.0 68-69 8.249474254660001E-4 0.0 0.0 8.400280989784612 0.0 70-71 8.249474254660001E-4 0.0 0.0 9.03038217910497 0.0 72-73 8.249474254660001E-4 0.0 0.0 9.618379320979429 0.0 74-75 8.249474254660001E-4 0.0 0.0 10.213864447177347 0.0 76-77 8.249474254660001E-4 0.0 0.0 10.903932968579657 0.0 78-79 8.249474254660001E-4 0.0 0.0 11.817562242283252 0.0 80-81 8.884049197326155E-4 0.0 0.0 13.810095833507841 0.0 82-83 8.884049197326155E-4 0.0 0.0 15.283007732930251 0.0 84-85 8.884049197326155E-4 0.0 0.0 16.00242534543087 0.0 86-87 8.884049197326155E-4 0.0 0.0 16.593151159558793 0.0 88-89 8.884049197326155E-4 0.0 0.0 17.25882027441559 0.0 90-91 8.884049197326155E-4 0.0 0.0 18.09144605668785 0.0 92-93 8.884049197326155E-4 0.0 0.0 19.14290500793853 0.0 94-95 8.884049197326155E-4 0.0 0.0 20.490646999920045 0.0 96-97 9.518624139992309E-4 0.0 0.0 22.020543729193875 0.0 98-99 0.0010153199082658462 0.0 0.0 23.592163760948004 0.0 100-101 0.0010153199082658462 0.0 0.0 24.98321549276648 0.0 102-103 0.0010153199082658462 0.0 0.0 26.171964732862985 0.0 104-105 0.001142234896799077 0.0 0.0 27.409195498579187 0.0 106-107 0.001142234896799077 0.0 0.0 28.57287902844038 0.0 108-109 0.001142234896799077 0.0 0.0 29.560658384194518 0.0 110-111 0.001142234896799077 0.0 0.0 30.6546021278567 0.0 112-113 0.001142234896799077 0.0 0.0 33.514694851947326 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACGG 505 0.0 89.7012 2 GAGTAGT 3135 0.0 88.596664 1 AGTAGTA 2610 0.0 88.15011 2 AGTCCGT 385 0.0 86.69685 2 GAGTTAG 1685 0.0 81.53234 1 GAGTACG 790 0.0 80.901886 1 GAGTCCG 865 0.0 80.792625 1 GAGTAAG 1280 0.0 80.26394 1 GAGTCAG 6980 0.0 78.643364 1 AGTTAGG 460 0.0 77.74446 2 GAGTAGG 1515 0.0 76.881874 1 TCGTAGG 1515 0.0 75.93128 2 GAGTCGC 590 0.0 73.90483 1 GAGTCGT 965 0.0 73.039314 1 AGTCGGT 425 0.0 72.92735 2 GAGTTGT 1085 0.0 71.56746 1 AGTAAGG 710 0.0 71.35701 2 AGTCGTA 560 0.0 71.312035 2 GAGTCGA 525 0.0 70.53982 1 AGTTGTA 755 0.0 69.47232 2 >>END_MODULE