FastQCFastQC Report
Thu 26 May 2016
SRR935910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616238
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT24360.39530181520776064No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA20280.3290936294094165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATCA8750.060.629591
GTAACGA401.7555579E-459.6077421
TCTACGG701.3400495E-859.50132
TGTTCGG453.1694307E-452.8900452
GTAACAA2500.052.454811
CGCAGAA5000.052.3611452
GGAAACA8000.052.1567731
GAATCAA11050.047.9241262
GTCTACG1004.0763553E-947.6861951
CTAGATA1400.046.743434
CTACGGG909.725045E-846.2712753
TAACAAC4450.045.542991
TAGTCAA1451.8189894E-1245.1242689
TACTAGG1204.1654857E-1044.6259732
AATCAAC12550.044.0854343
TAGGGTG951.4895522E-743.8288355
CCTCTAG1501.8189894E-1243.712341
GTATCGG1109.466021E-943.351081
ATCGGGT558.531229E-443.2666474
TATACGG853.4777277E-642.000922
Produced by FastQC (version 0.11.5)