##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935910_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 616238 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.586948549099535 31.0 26.0 33.0 7.0 34.0 2 26.727524105946078 31.0 26.0 34.0 7.0 34.0 3 26.6004400897056 31.0 26.0 34.0 7.0 34.0 4 29.351623885576675 35.0 28.0 37.0 7.0 37.0 5 29.85250666138732 35.0 31.0 37.0 7.0 37.0 6 29.87512941428474 36.0 31.0 37.0 7.0 37.0 7 29.86176607090118 37.0 31.0 37.0 2.0 37.0 8 29.395441371677826 37.0 30.0 37.0 2.0 37.0 9 30.17720750748899 39.0 27.0 39.0 2.0 39.0 10-11 30.428704494042886 38.5 28.0 39.0 2.0 39.0 12-13 30.42955562623532 38.0 28.5 39.0 2.0 39.0 14-15 31.290311535478175 39.0 27.5 41.0 2.0 41.0 16-17 31.578895653951882 38.5 28.5 41.0 2.0 41.0 18-19 31.947956795913267 39.0 30.0 41.0 2.0 41.0 20-21 32.23450030669969 39.0 30.0 41.0 2.0 41.0 22-23 32.0707105047076 39.0 30.0 41.0 2.0 41.0 24-25 31.869401919388288 38.0 30.0 41.0 2.0 41.0 26-27 31.702989753958697 38.0 30.0 41.0 2.0 41.0 28-29 31.42582817028486 38.0 30.0 41.0 2.0 41.0 30-31 31.107579376799222 38.0 30.0 41.0 2.0 41.0 32-33 30.820871968297965 38.0 30.0 41.0 2.0 41.0 34-35 30.37253220346684 38.0 28.5 40.0 2.0 41.0 36-37 30.041215407034294 38.0 27.0 40.5 2.0 41.0 38-39 29.822748678270408 38.0 27.0 41.0 2.0 41.0 40-41 29.476006023646708 38.0 25.5 41.0 2.0 41.0 42-43 29.080965471132906 37.0 23.0 41.0 2.0 41.0 44-45 28.763188086421156 37.0 20.5 40.0 2.0 41.0 46-47 28.453720802676887 36.5 12.0 40.0 2.0 41.0 48-49 28.07535400283657 36.0 4.5 40.0 2.0 41.0 50-51 27.706276633378664 35.0 2.0 40.0 2.0 41.0 52-53 27.37135165309507 35.0 2.0 40.0 2.0 41.0 54-55 27.026108419149743 35.0 2.0 39.5 2.0 41.0 56-57 26.66468069155099 35.0 2.0 39.0 2.0 41.0 58-59 26.278861413934226 35.0 2.0 39.0 2.0 41.0 60-61 25.92745010856195 35.0 2.0 38.0 2.0 41.0 62-63 25.54161379207384 35.0 2.0 37.0 2.0 40.0 64-65 25.191138813250724 34.0 2.0 37.0 2.0 40.0 66-67 24.91431962975344 34.0 2.0 36.0 2.0 39.0 68-69 24.68522551351913 34.0 2.0 35.5 2.0 39.0 70-71 24.35741547908438 34.0 2.0 35.0 2.0 38.5 72-73 24.02252457654348 34.0 2.0 35.0 2.0 37.0 74-75 23.654803014419755 34.0 2.0 35.0 2.0 37.0 76-77 23.315585861306833 33.0 2.0 35.0 2.0 36.0 78-79 23.05236450851781 33.0 2.0 35.0 2.0 36.0 80-81 22.776084889279794 33.0 2.0 35.0 2.0 35.0 82-83 22.486625297368874 33.0 2.0 35.0 2.0 35.0 84-85 22.192430521973655 32.0 2.0 35.0 2.0 35.0 86-87 21.926487006643537 32.0 2.0 35.0 2.0 35.0 88-89 21.636661484686112 31.5 2.0 35.0 2.0 35.0 90-91 21.364859031737737 31.0 2.0 35.0 2.0 35.0 92-93 21.0241035119548 30.5 2.0 35.0 2.0 35.0 94-95 20.680178762101654 30.0 2.0 35.0 2.0 35.0 96-97 20.37559352068519 29.0 2.0 35.0 2.0 35.0 98-99 20.057295233335175 29.0 2.0 35.0 2.0 35.0 100-101 19.788239284172676 27.0 2.0 35.0 2.0 35.0 102-103 19.134373407676904 25.5 2.0 34.0 2.0 35.0 104-105 19.42830205212921 27.0 2.0 35.0 2.0 35.0 106-107 19.366438778523882 27.0 2.0 35.0 2.0 35.0 108-109 19.184197501614634 26.0 2.0 35.0 2.0 35.0 110-111 18.940966801787624 25.0 2.0 35.0 2.0 35.0 112-113 18.639936193483685 25.0 2.0 35.0 2.0 35.0 114-115 18.249178888676127 23.5 2.0 34.5 2.0 35.0 116-117 17.794275912877815 21.0 2.0 34.0 2.0 35.0 118-119 17.162037232368014 12.0 2.0 34.0 2.0 35.0 120-121 16.469700018499346 2.0 2.0 34.0 2.0 35.0 122-123 15.780510938955404 2.0 2.0 34.0 2.0 35.0 124-125 13.713673126292115 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0981437216918728 1101 2 0.05949511162580379 1101 3 -0.2746735293487923 1101 4 -0.11317276084938044 1101 5 -0.14166104238514876 1101 6 -0.3213358481463686 1101 7 -0.3122950824755186 1101 8 -0.26986569118822246 1101 9 -0.31395383738805904 1101 10-11 -0.1408912432938081 1101 12-13 -0.17589649664805052 1101 14-15 -0.22154246966178803 1101 16-17 -0.44037168397863624 1101 18-19 -0.03645634312468715 1101 20-21 -0.32021428829424536 1101 22-23 -0.28824961028439233 1101 24-25 -0.32769115694587825 1101 26-27 -0.14000497749202978 1101 28-29 -0.22752233312350967 1101 30-31 -0.5747323407304812 1101 32-33 -0.4173120684959635 1101 34-35 -0.6002516026071483 1101 36-37 -0.4705838688359343 1101 38-39 -0.35537592294388887 1101 40-41 -0.4107946235308795 1101 42-43 -0.46837634643707204 1101 44-45 -0.1827149500002747 1101 46-47 -0.03553044842794151 1101 48-49 -0.3100454278551332 1101 50-51 -0.1306055878407797 1101 52-53 -0.1690118998798411 1101 54-55 -0.13162633755949926 1101 56-57 -0.07271598480911479 1101 58-59 -0.4251750093353479 1101 60-61 -0.5992093034995669 1101 62-63 -0.3059022034999934 1101 64-65 -0.2603859604303871 1101 66-67 -0.33052789867241117 1101 68-69 -0.36678732317332674 1101 70-71 -0.42295010412501455 1101 72-73 -0.34563521149864584 1101 74-75 -0.22659657447126946 1101 76-77 -0.06488795807152314 1101 78-79 0.05536182214915897 1101 80-81 0.008491494797038257 1101 82-83 -0.10025425109616748 1101 84-85 -0.07689923260264919 1101 86-87 -0.14313715971942642 1101 88-89 -0.19093933213717662 1101 90-91 -0.16898088455342375 1101 92-93 -0.2745017656054465 1101 94-95 -0.12568008472907266 1101 96-97 -0.13639046797867138 1101 98-99 -0.09587411147425229 1101 100-101 -0.015128965242222847 1101 102-103 0.010788851758661622 1101 104-105 -0.13674292165608648 1101 106-107 0.0877244602812759 1101 108-109 0.129107170590828 1101 110-111 -0.0288521552730181 1101 112-113 -0.1811907422107879 1101 114-115 -0.3086139241499275 1101 116-117 -0.2042077070985755 1101 118-119 -0.03535676289485323 1101 120-121 -0.4552166669279636 1101 122-123 -0.3435239226027278 1101 124-125 -0.21213809825469276 1102 1 -0.06595565659241487 1102 2 0.24056293888543578 1102 3 0.14345562748450646 1102 4 -0.07650374439062091 1102 5 0.18017976430869354 1102 6 0.12196877685746443 1102 7 0.05743550875260439 1102 8 0.1133935726116988 1102 9 0.02146161607073438 1102 10-11 0.33653542786811386 1102 12-13 0.3887845956987803 1102 14-15 0.13685241800793335 1102 16-17 0.214903088449109 1102 18-19 0.036352986571575485 1102 20-21 0.018992503626556356 1102 22-23 -0.10886443849415883 1102 24-25 -0.33889966523656057 1102 26-27 -0.4764671100816038 1102 28-29 -0.6167199302056794 1102 30-31 -0.520755573022754 1102 32-33 -0.42866616173162697 1102 34-35 -0.3583154760861653 1102 36-37 -0.1269313724372445 1102 38-39 -0.038621394485865324 1102 40-41 0.03997314549477338 1102 42-43 0.03912357693927149 1102 44-45 0.03442422920713284 1102 46-47 0.07230968397773552 1102 48-49 0.07337321314771827 1102 50-51 -0.03387282050084295 1102 52-53 0.2161169811082999 1102 54-55 0.12482230926675086 1102 56-57 0.3233470917838943 1102 58-59 0.2961937956421252 1102 60-61 0.28474723556317727 1102 62-63 0.2046074970547629 1102 64-65 0.02725924139632241 1102 66-67 -0.07523167162119648 1102 68-69 0.15700125683709487 1102 70-71 0.09213096370224605 1102 72-73 -0.2115223601791847 1102 74-75 -0.055367224546358784 1102 76-77 0.016305531982727217 1102 78-79 -0.03413000976919278 1102 80-81 -0.04923983201818061 1102 82-83 0.0765379762002425 1102 84-85 0.02951110239597199 1102 86-87 0.1977109108969337 1102 88-89 -6.96588397328668E-4 1102 90-91 0.19069821557836875 1102 92-93 0.22731421502409432 1102 94-95 0.22774192771461088 1102 96-97 0.07484409235479461 1102 98-99 -0.07325021102370499 1102 100-101 0.06903905510901964 1102 102-103 0.11230446765960522 1102 104-105 0.13452474009644533 1102 106-107 -0.010991327256650862 1102 108-109 0.09333311842544845 1102 110-111 0.19774391546594217 1102 112-113 0.3978373633459604 1102 114-115 0.12841369101542455 1102 116-117 -0.100732058096213 1102 118-119 -0.5207032550639141 1102 120-121 -0.8072134793839076 1102 122-123 -0.8649172471498829 1102 124-125 -0.7603113903134169 1103 1 0.033977274326428386 1103 2 0.21008345179339116 1103 3 -0.06630008191312342 1103 4 -0.05586010856436374 1103 5 0.06987101918256755 1103 6 0.010449377978311247 1103 7 -0.06338262036106102 1103 8 0.07896951413655628 1103 9 -0.054261081697799085 1103 10-11 0.24845326255987743 1103 12-13 0.053480397629005694 1103 14-15 0.05464163921085685 1103 16-17 -0.1043997341361198 1103 18-19 -0.2015674740452269 1103 20-21 -0.2115986705370716 1103 22-23 -0.12169140026477976 1103 24-25 0.004924579667896722 1103 26-27 -0.27836891639973516 1103 28-29 -0.056701628303873264 1103 30-31 -0.09135146475734146 1103 32-33 0.04967848240437789 1103 34-35 -0.2929018076347809 1103 36-37 -0.15256966547229212 1103 38-39 0.04598795653551946 1103 40-41 0.2346383116328461 1103 42-43 0.23226547920029716 1103 44-45 0.1869424458516349 1103 46-47 0.09920836419463086 1103 48-49 0.22456709963045896 1103 50-51 0.24282550504565492 1103 52-53 0.19239981938087425 1103 54-55 0.09927637831379954 1103 56-57 0.08632751670631222 1103 58-59 -0.049433641295884456 1103 60-61 -0.19075265644423567 1103 62-63 -0.28600709444906514 1103 64-65 -0.1462464822108558 1103 66-67 0.09028686331724955 1103 68-69 0.2439736562050534 1103 70-71 0.2080922682527131 1103 72-73 0.03295722922162625 1103 74-75 0.008112524001425925 1103 76-77 0.18850539784456544 1103 78-79 0.24302302141224885 1103 80-81 -0.03540967594046762 1103 82-83 -0.0999972236997202 1103 84-85 0.015644001485426884 1103 86-87 -0.010360966696310925 1103 88-89 0.0013439031394604228 1103 90-91 -0.22678976314140797 1103 92-93 -0.0931637374364307 1103 94-95 -0.08879145336281269 1103 96-97 -0.2817104893728626 1103 98-99 0.04821871552996981 1103 100-101 -0.08550544352117839 1103 102-103 -0.19813727886473842 1103 104-105 -0.3154349047784528 1103 106-107 -0.13793519384601538 1103 108-109 -0.0031326820269867994 1103 110-111 -8.970830676346964E-4 1103 112-113 -0.06198797285201607 1103 114-115 0.2062223508842429 1103 116-117 0.21262833022240102 1103 118-119 0.057119614355993775 1103 120-121 -0.06855980593075728 1103 122-123 -0.18571207259975786 1103 124-125 -0.18227537762712842 1104 1 0.4343750312382717 1104 2 0.3447828518977225 1104 3 0.34800286540038883 1104 4 0.3763255982692293 1104 5 0.5819178371272749 1104 6 0.27596841814524 1104 7 0.29944014959199095 1104 8 0.2969533430358773 1104 9 0.4201618447497779 1104 10-11 0.3938383042917515 1104 12-13 0.4505363444208683 1104 14-15 0.38010134187126354 1104 16-17 0.36074763153941447 1104 18-19 0.3366595734247788 1104 20-21 0.32554432633303776 1104 22-23 0.2032222017174803 1104 24-25 0.2779830694957539 1104 26-27 0.7014009062398081 1104 28-29 0.432254096787414 1104 30-31 0.4918596072301398 1104 32-33 0.6178921626339005 1104 34-35 0.6030374855316261 1104 36-37 0.5242854466821179 1104 38-39 0.24571409112080644 1104 40-41 0.47869641022439424 1104 42-43 0.37731633992017066 1104 44-45 0.15995529144370124 1104 46-47 0.2397274043615596 1104 48-49 0.24306931663619835 1104 50-51 0.20866568501435623 1104 52-53 0.28966279538504836 1104 54-55 0.5250148368427503 1104 56-57 0.4250321985007801 1104 58-59 0.39590738635147815 1104 60-61 0.6751592501806982 1104 62-63 0.5909083333026324 1104 64-65 0.3516261703713006 1104 66-67 0.3581207182989914 1104 68-69 0.5360886144731793 1104 70-71 0.6389331002819247 1104 72-73 0.3513013215533931 1104 74-75 0.5725361025095097 1104 76-77 0.48381545261764813 1104 78-79 0.6507483083188745 1104 80-81 0.6167486443516559 1104 82-83 0.41679333446407796 1104 84-85 0.27203419449533683 1104 86-87 0.164700979052256 1104 88-89 0.3025469547826525 1104 90-91 0.35796180180381754 1104 92-93 0.2393871232834428 1104 94-95 0.24265090448799143 1104 96-97 0.2588802774503023 1104 98-99 0.21951501182626387 1104 100-101 0.1278650572612925 1104 102-103 0.24567955733765956 1104 104-105 0.022528058184512645 1104 106-107 0.13987518695732248 1104 108-109 0.46106919590728523 1104 110-111 0.24550028260789958 1104 112-113 0.14595412417458675 1104 114-115 0.13656598677365395 1104 116-117 -0.11821087165553479 1104 118-119 -0.0886077591442671 1104 120-121 -0.053578715685585365 1104 122-123 -0.020145758045766016 1104 124-125 -0.27578085493542304 1105 1 -0.2575656339166805 1105 2 -0.18226268854067484 1105 3 -0.29261032431994494 1105 4 -0.11876132538912287 1105 5 -0.0457512860775644 1105 6 -0.08929991140880489 1105 7 -0.13628830845789608 1105 8 0.014734900103146487 1105 9 0.05883814860465009 1105 10-11 -0.10270128376569687 1105 12-13 -0.14448916578321302 1105 14-15 -0.20160217286796467 1105 16-17 -0.17217942817336152 1105 18-19 -0.14848309475360466 1105 20-21 -0.03163396164882215 1105 22-23 0.24601084495255066 1105 24-25 0.10221067720550536 1105 26-27 0.243516363533832 1105 28-29 0.17673869618272775 1105 30-31 0.20285025348864139 1105 32-33 0.3921859079097878 1105 34-35 0.21472730034644272 1105 36-37 0.28933174297841546 1105 38-39 0.07196125665369024 1105 40-41 -0.010355460523907567 1105 42-43 -0.1409659730049988 1105 44-45 -0.28957938353740076 1105 46-47 -0.3667540771199249 1105 48-49 -0.3341462918294127 1105 50-51 -0.28584253494029 1105 52-53 -0.2989969740782925 1105 54-55 -0.23536639898491174 1105 56-57 -0.2718333532754862 1105 58-59 -0.22694314274905025 1105 60-61 -0.36408630915414975 1105 62-63 -0.21498972036328112 1105 64-65 -0.5743588069529615 1105 66-67 -0.34676401337145535 1105 68-69 -0.48735479066665377 1105 70-71 -0.43831793902268146 1105 72-73 -0.32631347436497293 1105 74-75 -0.2417118188812708 1105 76-77 -0.3674813160898367 1105 78-79 -0.4120941897914818 1105 80-81 -0.274171612640032 1105 82-83 -0.3669840653847487 1105 84-85 -0.442346002027719 1105 86-87 -0.44529996577011843 1105 88-89 -0.24117470054614287 1105 90-91 -0.09916688300343779 1105 92-93 0.012913200380946677 1105 94-95 -0.22352445454450987 1105 96-97 -0.21234988127985588 1105 98-99 -0.20631643186232296 1105 100-101 -0.3773078452330374 1105 102-103 -0.12002575256407866 1105 104-105 -0.4123736802886562 1105 106-107 -0.3099075038967989 1105 108-109 -0.3391008721199249 1105 110-111 -0.456998772569726 1105 112-113 -0.35517966267348555 1105 114-115 -0.07717645464735767 1105 116-117 -0.2682250439911371 1105 118-119 0.0695673164415247 1105 120-121 -0.22036348514892623 1105 122-123 -0.13880363786435979 1105 124-125 -0.11761758962930102 1106 1 -0.27263921693451465 1106 2 -0.15955678430167097 1106 3 -0.11989511850369183 1106 4 -0.622524693263987 1106 5 -0.28522667712089955 1106 6 -0.3269099591233804 1106 7 -0.38692810613601836 1106 8 -0.17020810888167404 1106 9 -0.1947756398523417 1106 10-11 -0.20889764257529464 1106 12-13 -0.036706510962233097 1106 14-15 -0.17042680545566213 1106 16-17 -0.07055215494330014 1106 18-19 -0.009179405798349194 1106 20-21 0.002054088589751757 1106 22-23 -0.08360311445234458 1106 24-25 0.07570495296458546 1106 26-27 -0.1861611985479108 1106 28-29 -0.20028188292844717 1106 30-31 -0.15567904145180478 1106 32-33 -0.11829722104024043 1106 34-35 -0.051532862255186274 1106 36-37 -0.062294273281750634 1106 38-39 -0.004994211416761374 1106 40-41 0.017372038457949657 1106 42-43 -0.24646722746095762 1106 44-45 0.007725421607126748 1106 46-47 -0.03434461666168431 1106 48-49 -0.002863076399854947 1106 50-51 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0.5596040646177514 2215 98-99 0.6334299831355992 2215 100-101 0.3209499555398523 2215 102-103 0.20374065540756092 2215 104-105 0.328931292404647 2215 106-107 0.38002255263285534 2215 108-109 0.03278281093075819 2215 110-111 0.15426723618119098 2215 112-113 0.3320261116550256 2215 114-115 0.32312375933494764 2215 116-117 0.20065649923648365 2215 118-119 0.2409224312574061 2215 120-121 0.15033690768426666 2215 122-123 -0.03125197870304319 2215 124-125 -0.2458904011013079 2216 1 -0.09071756135432096 2216 2 -0.298739227874357 2216 3 0.05556340671093096 2216 4 -0.1687456268019929 2216 5 -0.39147603751660043 2216 6 -0.6488463966695726 2216 7 -0.5647585683567264 2216 8 -0.4187589076763736 2216 9 -1.1194001210621884 2216 10-11 -0.9747211686427235 2216 12-13 -0.8173375872030633 2216 14-15 -1.0245674615475373 2216 16-17 -0.8076355593437761 2216 18-19 -1.0426815946663908 2216 20-21 -0.9837131522994405 2216 22-23 -1.0635530831828035 2216 24-25 -0.8510179988803159 2216 26-27 -1.0436596350707354 2216 28-29 -1.0168894217311042 2216 30-31 -0.932590179094646 2216 32-33 -1.0500903872236513 2216 34-35 -0.9891188534997148 2216 36-37 -1.1168221031754335 2216 38-39 -1.681166250759535 2216 40-41 -1.7335632051602232 2216 42-43 -1.6320212669174374 2216 44-45 -1.6263890817756703 2216 46-47 -1.8849592053250532 2216 48-49 -1.8005809682640894 2216 50-51 -1.791553332079662 2216 52-53 -1.7090248826776318 2216 54-55 -1.6428110178581043 2216 56-57 -1.4910379581943758 2216 58-59 -1.789495035300945 2216 60-61 -1.6172748666284171 2216 62-63 -1.5847629629936648 2216 64-65 -1.260697547577415 2216 66-67 -1.4554191677408959 2216 68-69 -1.5350313997718317 2216 70-71 -1.3753421846183578 2216 72-73 -1.436524533147459 2216 74-75 -1.2908257208523146 2216 76-77 -1.3726321969550028 2216 78-79 -1.5920383725928104 2216 80-81 -1.6057798456768388 2216 82-83 -1.6530001128293677 2216 84-85 -1.5274672300060885 2216 86-87 -1.6634649753636985 2216 88-89 -1.5340876606019584 2216 90-91 -1.5541642665722506 2216 92-93 -1.3203992015028838 2216 94-95 -1.2535314316943449 2216 96-97 -1.301226560156536 2216 98-99 -1.3540889767777244 2216 100-101 -1.2568037461575088 2216 102-103 -1.1639660979329953 2216 104-105 -1.6469733663169137 2216 106-107 -1.626485211903077 2216 108-109 -1.93828977845169 2216 110-111 -1.725414959557341 2216 112-113 -1.911823653603193 2216 114-115 -1.7067281727705073 2216 116-117 -1.9293326665273298 2216 118-119 -1.9830717904832973 2216 120-121 -1.6272290005858672 2216 122-123 -1.3637835785585875 2216 124-125 -0.8564646156191849 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64849.0 3 10212.0 4 2873.0 5 8357.0 6 17607.0 7 16108.0 8 13665.0 9 10043.0 10 8318.0 11 6522.0 12 5426.0 13 4656.0 14 4266.0 15 4339.0 16 4339.0 17 4628.0 18 4876.0 19 5290.0 20 5815.0 21 6309.0 22 6918.0 23 7452.0 24 7914.0 25 8173.0 26 8563.0 27 8986.0 28 9968.0 29 11812.0 30 14497.0 31 18307.0 32 23307.0 33 29742.0 34 40721.0 35 54772.0 36 82134.0 37 63008.0 38 11433.0 39 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 59.06531302766488 17.18625320763364 9.574065256147524 14.174368508553961 2 16.504397418190493 30.105151771382943 36.777477168968296 16.612973641458264 3 32.610343221501125 26.49178582614806 19.98081763609011 20.917053316260706 4 13.496463546451048 28.235149004956554 31.133937294897056 27.134450153695344 5 18.273195667167023 37.306178749645014 20.967666031076313 23.452959552111647 6 28.36874713990374 42.10370019375631 11.706678263917512 17.82087440242244 7 31.79198912106868 41.235920595485176 9.307620282456263 17.66447000098988 8 33.69169054813238 33.7684466066682 11.431135373021464 21.108727472177957 9 39.1207617835966 20.260191679188885 11.437301821698759 29.181744715515755 10-11 30.902102758998957 32.330122452688734 14.095446889026642 22.672327899285666 12-13 33.00437493306158 32.22196618838825 16.33459799622873 18.43906088232144 14-15 34.721081789827956 26.407086223180006 19.685251477513557 19.186580509478482 16-17 30.695770140757304 28.68177554775915 20.38984937637731 20.23260493510624 18-19 30.08334442212262 27.705285944716163 21.007224481450347 21.204145151710865 20-21 38.61746597905355 23.907483796844726 18.06899282420104 19.406057399900686 22-23 35.2347631921433 25.77461954634411 18.679958068148995 20.310659193363602 24-25 27.145112764873314 29.746786144314374 20.990023335139995 22.11807775567232 26-27 24.911498644588455 31.24878800000325 20.8114294338104 23.0282839215979 28-29 24.22352789392414 31.8703895891029 21.699511766056663 22.206570750916303 30-31 23.082721286256284 33.14084817878807 21.885213180621772 21.89121735433388 32-33 22.442789961021553 34.07904089004573 21.451452198663503 22.026716950269215 34-35 22.20051343798987 34.18103070566892 21.188079930156857 22.430375926184364 36-37 21.320415164270948 35.63858444302363 20.46774135966948 22.573259033035935 38-39 20.581090422856104 36.42537461175714 21.0529860216345 21.94054894375225 40-41 19.760465923880062 36.90749353334264 21.619974750015416 21.712065792761887 42-43 18.90024633339716 37.33565602900178 21.615187638542253 22.148909999058805 44-45 18.20927953160954 37.30604084785424 22.23564596795394 22.24903365258228 46-47 17.705821452101297 37.468964912907026 21.962618339018366 22.86259529597331 48-49 16.93347375526988 37.39902440290926 22.06444587967636 23.603055962144495 50-51 16.761705704614126 37.387340605415446 21.86695724703767 23.98399644293276 52-53 16.897854400410232 36.41750427594533 21.748658797412688 24.935982526231747 54-55 16.450786871306217 35.20977284750372 21.939818706408886 26.39962157478117 56-57 16.877381392843002 33.89642929007718 21.990195314782 27.23599400229782 58-59 17.705010077275336 32.67730974071706 21.979900622811314 27.637779559196286 60-61 18.020716022056412 31.668770832048654 21.55360428925188 28.756908856643047 62-63 18.94260009931228 29.793927021702654 22.03231543656834 29.231157442416727 64-65 19.57619754296485 28.70516795257619 23.040810216307047 28.677824288151914 66-67 19.891372962025976 28.214631708255105 23.195458890004982 28.69853643971394 68-69 20.34176799211305 27.962496399345998 23.522190171330273 28.173545437210677 70-71 20.744867321269172 27.753956017203603 23.515864643349836 27.985312018177392 72-73 21.115786433163812 26.60165390644524 24.205582907902468 28.076976752488488 74-75 21.373701408560418 25.66713702528333 25.363986974934132 27.595174591222115 76-77 21.601337152222772 25.116879112676173 25.999334669403716 27.28244906569734 78-79 22.076182346908308 24.515361097768373 26.946669436186372 26.461787119136947 80-81 22.5425285565687 24.421687900518148 27.05502626441622 25.98075727849693 82-83 22.848801923931987 24.084038958973643 26.846932516332977 26.220226600761393 84-85 22.636473557360688 24.46997054264824 26.86210226488895 26.031453635102125 86-87 22.35804912325115 24.58288402540953 26.720169807968023 26.3388970433713 88-89 22.23635125737142 25.315570241817493 26.24570617539822 26.20237232541287 90-91 23.135641696586138 26.188685368871578 25.206677620235705 25.468995314306582 92-93 23.353955213949305 27.18444789945697 24.54095758665572 24.920639299938006 94-95 22.51213946755127 28.29989029037347 24.056594587225643 25.13137565484962 96-97 21.726856990775904 29.840947595957235 23.204108326355215 25.22808708691165 98-99 21.1146045900256 31.281385527260632 22.557415889423954 25.046593993289818 100-101 20.77772341535568 33.26761159915584 21.860833822189445 24.093831163299036 102-103 20.425728716606827 35.66587557556949 21.471023753017302 22.437371954806384 104-105 19.469196004770907 37.56789211907795 21.11564581693672 21.847266059214423 106-107 18.507706101187456 38.818303156681935 20.981204334403007 21.692786407727603 108-109 18.245547986329957 40.09286996257939 20.671558715950656 20.990023335139995 110-111 18.324981581791448 41.31390793816675 19.929962125023124 20.431148355018678 112-113 18.627045289687505 42.18380060788695 19.594942172846956 19.594211929578584 114-115 18.506566744183406 42.56959061100824 19.743561864361396 19.180280780446957 116-117 18.432894433644144 43.01406274848354 19.426422908032286 19.126619909840027 118-119 18.32335582164058 43.30819265623744 19.249790719899188 19.118660802222795 120-121 18.819175616916073 43.1065972878655 18.975286033391725 19.0989410618267 122-123 19.472325316083886 42.80524753616039 18.800479704258105 18.921947443497622 124-125 19.468514839245906 44.74894905787277 18.074740710680047 17.707795392201273 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 57.0 1 85.0 2 130.5 3 146.0 4 145.5 5 139.5 6 144.5 7 162.5 8 166.0 9 155.5 10 160.5 11 173.0 12 189.5 13 228.0 14 266.5 15 318.0 16 389.0 17 478.0 18 600.0 19 767.5 20 1010.5 21 1302.5 22 1674.5 23 2196.0 24 2811.0 25 3528.5 26 4289.5 27 5072.0 28 5906.5 29 6770.0 30 7614.0 31 8385.5 32 9079.5 33 9404.5 34 9707.0 35 10012.0 36 10058.0 37 9899.0 38 9606.0 39 9342.0 40 8962.0 41 8799.0 42 8681.5 43 8780.0 44 9289.0 45 10149.5 46 11914.0 47 14394.5 48 17948.0 49 23342.5 50 30365.0 51 37379.5 52 43059.5 53 45440.0 54 43016.0 55 37461.0 56 30424.0 57 23547.5 58 17748.5 59 13118.0 60 9425.5 61 6479.0 62 4662.0 63 3622.0 64 2929.5 65 2353.5 66 1892.5 67 1647.5 68 1432.0 69 1216.5 70 974.5 71 708.0 72 533.0 73 429.5 74 363.5 75 324.0 76 269.5 77 182.5 78 121.0 79 85.0 80 61.0 81 44.0 82 28.0 83 22.5 84 22.0 85 11.0 86 5.5 87 5.0 88 5.5 89 5.5 90 4.5 91 3.0 92 2.5 93 2.0 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14750794335954615 2 0.013144272180553616 3 0.0076269233640249385 4 0.013631097076129675 5 0.0021095745474962596 6 0.0 7 1.6227496519201996E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.302373433640899E-4 28-29 0.006085311194700749 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.245499303840399E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0012170622389401497 66-67 1.6227496519201996E-4 68-69 0.004949386438356609 70-71 0.014280196936897758 72-73 0.0 74-75 0.006734411055468828 76-77 4.8682489557605994E-4 78-79 9.736497911521199E-4 80-81 8.113748259600998E-4 82-83 0.0 84-85 0.015091571762857858 86-87 0.0020284370649002494 88-89 0.014848159315069828 90-91 8.113748259600998E-5 92-93 0.010385597772289278 94-95 0.010710147702673319 96-97 0.004219149094992519 98-99 0.0017038871345162094 100-101 5.679623781720699E-4 102-103 8.113748259600998E-5 104-105 4.8682489557605994E-4 106-107 8.925123085561098E-4 108-109 0.0 110-111 0.0 112-113 8.113748259600998E-4 114-115 0.004381424060184539 116-117 0.0 118-119 0.07083302230631672 120-121 0.0012170622389401497 122-123 0.004056874129800499 124-125 0.23245888763756858 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 616238.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.66878772923977 #Duplication Level Percentage of deduplicated Percentage of total 1 86.97085686511873 64.94008449891645 2 7.680411452459304 11.469740248338116 3 2.409385593790564 5.397177043819077 4 1.0943364744314126 3.2685111165473506 5 0.5836803006205266 2.1791350234386475 6 0.35309053466737594 1.581890530936924 7 0.22948324171690446 1.1994664824224153 8 0.15305787273205168 0.914291664745485 9 0.09680213040481596 0.6505287954241851 >10 0.3977690537900665 4.951600081684019 >50 0.017195799949915873 0.8944029880131618 >100 0.013495346558487626 1.8276410851834095 >500 0.0 0.0 >1k 4.353337599061327E-4 0.7255304405307996 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 2436 0.39530181520776064 No Hit GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA 2028 0.3290936294094165 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.6227496519201996E-4 0.0 12-13 1.6227496519201996E-4 0.0 0.0 6.490998607680798E-4 0.0 14-15 2.4341244778802994E-4 0.0 0.0 0.0011359247563441396 0.0 16-17 4.056874129800499E-4 0.0 0.0 0.0014604746867281798 0.0 18-19 0.0012981997215361597 0.0 0.0 0.0029209493734563595 0.0 20-21 0.0018661620997082297 0.0 0.0 0.006572136090276809 0.0 22-23 0.0021095745474962596 0.0 0.0 0.017687971205930176 0.0 24-25 0.0029209493734563595 0.0 0.0 0.036511867168204495 0.0 26-27 0.003894599164608479 0.0 0.0 0.07789198329216958 0.0 28-29 0.0043002865775885295 0.0 0.0 0.21395954160567832 0.0 30-31 0.005355073851336659 0.0 0.0 0.5461363953537433 0.0 32-33 0.006328723642488779 0.0 0.0 1.0415618640849802 0.0 34-35 0.006490998607680799 0.0 0.0 1.6917976496094043 0.0 36-37 0.006815548538064839 0.0 0.0 2.772143230375277 0.0 38-39 0.007140098468448879 0.0 0.0 4.412661990984003 0.0 40-41 0.007140098468448879 0.0 0.0 6.183325273676729 0.0 42-43 0.007140098468448879 0.0 0.0 7.530775447148667 0.0 44-45 0.007140098468448879 0.0 0.0 8.808285110622844 0.0 46-47 0.007140098468448879 0.0 0.0 10.29910521584193 0.0 48-49 0.007221235951044888 0.0 0.0 11.977920868235973 0.0 50-51 0.007302373433640898 0.0 0.0 13.826638409186062 0.0 52-53 0.007302373433640898 0.0 0.0 15.34610004576154 0.0 54-55 0.007302373433640898 0.0 0.0 16.630506395256376 0.0 56-57 0.007302373433640898 0.0 0.0 18.058526088946152 0.0 58-59 0.007302373433640898 0.0 0.0 19.608819968908115 0.0 60-61 0.007302373433640898 0.0 0.0 21.331774411834388 0.0 62-63 0.007302373433640898 0.0 0.0 22.932454668488475 0.0 64-65 0.007302373433640898 0.0 0.0 24.343841178246066 0.0 66-67 0.007302373433640898 0.0 0.0 25.75181991373463 0.0 68-69 0.007302373433640898 0.0 0.0 27.344467559611708 0.0 70-71 0.007464648398832918 0.0 0.0 29.06782768995096 0.0 72-73 0.007464648398832918 0.0 0.0 30.767089987959196 0.0 74-75 0.007464648398832918 0.0 0.0 32.348540661238026 0.0 76-77 0.007464648398832918 0.0 0.0 33.87311395921706 0.0 78-79 0.007464648398832918 0.0 0.0 35.490183987355536 0.0 80-81 0.007464648398832918 0.0 0.0 37.101655529194886 0.0 82-83 0.007464648398832918 0.0 0.0 38.682538240095546 0.0 84-85 0.007464648398832918 0.0 0.0 40.26407005085697 0.0 86-87 0.007464648398832918 0.0 0.0 41.671156274036974 0.0 88-89 0.007464648398832918 0.0 0.0 43.071913773574494 0.0 90-91 0.007464648398832918 0.0 0.0 44.48394938319286 0.0 92-93 0.007464648398832918 0.0 0.0 45.87894612146606 0.0 94-95 0.007464648398832918 0.0 0.0 47.214387947513785 0.0 96-97 0.007464648398832918 0.0 0.0 48.45067976982919 0.0 98-99 0.007464648398832918 0.0 0.0 49.62506369292383 0.0 100-101 0.007464648398832918 0.0 0.0 50.75076512646088 0.0 102-103 0.007464648398832918 0.0 0.0 51.84482294178548 0.0 104-105 0.007464648398832918 0.0 0.0 52.85441663772763 0.0 106-107 0.007464648398832918 0.0 0.0 53.79780214787144 0.0 108-109 0.007464648398832918 0.0 0.0 54.71611617589308 0.0 110-111 0.007464648398832918 0.0 0.0 55.553617271249095 0.0 112-113 0.007464648398832918 0.0 0.0 56.34073198991299 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAATCA 875 0.0 60.62959 1 GTAACGA 40 1.7555579E-4 59.607742 1 TCTACGG 70 1.3400495E-8 59.5013 2 TGTTCGG 45 3.1694307E-4 52.890045 2 GTAACAA 250 0.0 52.45481 1 CGCAGAA 500 0.0 52.361145 2 GGAAACA 800 0.0 52.156773 1 GAATCAA 1105 0.0 47.924126 2 GTCTACG 100 4.0763553E-9 47.686195 1 CTAGATA 140 0.0 46.74343 4 CTACGGG 90 9.725045E-8 46.271275 3 TAACAAC 445 0.0 45.54299 1 TAGTCAA 145 1.8189894E-12 45.124268 9 TACTAGG 120 4.1654857E-10 44.625973 2 AATCAAC 1255 0.0 44.085434 3 TAGGGTG 95 1.4895522E-7 43.828835 5 CCTCTAG 150 1.8189894E-12 43.71234 1 GTATCGG 110 9.466021E-9 43.35108 1 ATCGGGT 55 8.531229E-4 43.266647 4 TATACGG 85 3.4777277E-6 42.00092 2 >>END_MODULE