FastQCFastQC Report
Thu 26 May 2016
SRR935910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616238
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA24800.4024419136762095No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT19900.32292718073211973No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT16530.268240517462409No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT10580.17168691317315712RNA PCR Primer, Index 27 (95% over 23bp)
GTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.16032766560971573No Hit
GGTATCAACGCAGAGTGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.1324163715966883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGG507.387127E-659.5822752
GGTATCA114500.054.3328551
GTAATAT453.1494812E-452.957723
GCGTCAG350.00775457151.0663724
GTATAGT952.5993359E-950.170473
GTAAGGT1850.048.306033
GTTCGCG751.4355082E-647.7628561
ACGATTT505.326365E-447.607795
GTCGGTT255.334852E-447.59620714-15
GTCTAAG1301.8189894E-1145.9258231
TAAGGTT2050.043.5932434
GTTCGGT558.470908E-443.3290443
GTACGAT558.470908E-443.3290443
GTCGATT355.454771E-542.4966114-15
TGGTCGG1603.6379788E-1240.902999
GTATCAA174800.040.5424081
GTATAGG2700.039.8023761
GTGATCG1508.54925E-1139.6635068
GTCGACG1202.0747393E-839.66358
TATATGG2150.038.797762