FastQCFastQC Report
Thu 26 May 2016
SRR935908_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935908_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117959
Sequences flagged as poor quality0
Sequence length125
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA5870.49763053264269785No Hit
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT5750.4874575064217228No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT4820.4086165532091659No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC3730.3162115650353089No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA3080.2611076730050272No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC2450.20769928534490797No Hit
GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT2030.17209369357149518No Hit
GTGGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCC2010.17039818920133268No Hit
GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT1710.14496562364889495No Hit
ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA1600.13564034961300114No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA1490.1263150755771073No Hit
TATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTC1460.12377181902186354No Hit
ATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAATTGCCC1420.12038081028153849No Hit
GGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAAT1280.10851227969040089No Hit
ATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCC1240.10512127095007587No Hit
GTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAATT1210.10257801439483209No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTAA208.470262E-489.229658
TAGATGG453.972111E-666.096044
TAACAAC300.00419554259.612791
TTCACAG150.00422909959.51166524-25
GCACGTA150.00422909959.511665114-115
ACGTAGT150.00422909959.511665116-117
AGTAGAT507.4207856E-659.4864352
CACAAAG401.7674279E-459.4864358
GTCTGGG655.368438E-755.0271871
GTAAGAG453.1628157E-452.8768353
GTCATAG350.007720961751.0966721
GTGGAAT350.007720961751.0966721
GTACTAG350.007720961751.0966721
GAAAACA708.952975E-751.0966721
GATGGGT350.007786036550.9883736
TACTAGG350.007786036550.9883732
TCTATGG350.007786036550.9883732
GTCCTTC255.329924E-447.58914654-55
AGTAAGA505.3190195E-447.5891462
AGATGGG653.505606E-545.7587975