SRR935908_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR935908_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117959
Sequences flagged as poor quality	0
Sequence length	125
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT	1445	1.2250019074424163	No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	813	0.689222526471062	RNA PCR Primer, Index 17 (95% over 21bp)
ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA	583	0.49423952390237286	No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT	575	0.4874575064217228	RNA PCR Primer, Index 17 (95% over 23bp)
GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT	549	0.46541594960961014	No Hit
GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT	444	0.3764019701760781	No Hit
TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC	388	0.32892784781152773	No Hit
GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA	336	0.2848447341873024	No Hit
ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC	298	0.2526301511542146	No Hit
ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA	180	0.15259539331462627	No Hit
GTGGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCC	174	0.14750888020413874	No Hit
GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA	172	0.14581337583397622	No Hit
GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT	169	0.14327011927873246	No Hit
GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT	156	0.13224934087267612	No Hit
ATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCC	148	0.12546732339202604	No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	148	0.12546732339202604	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG	144	0.122076314651701	TruSeq Adapter, Index 12 (95% over 23bp)
TATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTC	143	0.12122856246661974	No Hit
ATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAATTGCCC	139	0.11783755372629472	No Hit
GGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAAT	137	0.1161420493561322	No Hit
GTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAATT	129	0.10936003187548216	No Hit
AGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAATTGC	121	0.10257801439483209	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTGG	20	8.3785283E-4	89.47439	1
CTTTACA	30	0.0041852943	59.649597	1
TACACGG	40	1.762047E-4	59.523113	2
TAGAGCT	15	0.0042329812	59.497883	54-55
TCCATAG	15	0.0042329812	59.497883	52-53
CTCCGCA	15	0.0042329812	59.497883	90-91
ATGTGTA	30	0.004227741	59.497883	8
AAGAATC	15	0.0042329812	59.497883	70-71
GTAGGAG	30	0.004227741	59.497883	8
CCATAGG	15	0.0042329812	59.497883	54-55
GTCCATA	15	0.0042329812	59.497883	52-53
GGGGCGC	30	0.004227741	59.497883	6
GAGGTTT	20	1.7693937E-4	59.49788	14-15
GTACACG	45	3.120299E-4	53.021862	1
AGTCCGT	35	0.0077801165	50.998184	6
GTCCGTG	35	0.0077801165	50.998184	7
TACACGT	35	0.0077801165	50.998184	5
ACACGGG	50	5.302871E-4	47.618492	3
TCTACAC	50	5.302871E-4	47.618492	3
AGTATGG	65	3.501625E-5	45.7676	7

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content