##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR935908_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117959 Sequences flagged as poor quality 0 Sequence length 125 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30476691053671 33.0 31.0 34.0 30.0 34.0 2 32.417721411676936 34.0 31.0 34.0 31.0 34.0 3 32.38521859290093 34.0 31.0 34.0 30.0 34.0 4 35.89398858925559 37.0 35.0 37.0 35.0 37.0 5 35.90116905026323 37.0 35.0 37.0 35.0 37.0 6 35.978746852720015 37.0 35.0 37.0 35.0 37.0 7 35.97693266304394 37.0 35.0 37.0 35.0 37.0 8 36.00650225925958 37.0 35.0 37.0 35.0 37.0 9 37.64291830212192 39.0 37.0 39.0 35.0 39.0 10-11 37.58895039801965 39.0 37.0 39.0 35.0 39.0 12-13 37.5529209301537 39.0 37.0 39.0 35.0 39.0 14-15 38.633177629515345 40.0 38.0 41.0 34.0 41.0 16-17 38.54314634745971 40.0 38.0 41.0 34.0 41.0 18-19 38.63482650751533 40.0 38.0 41.0 34.0 41.0 20-21 38.767046177061516 40.0 38.0 41.0 34.5 41.0 22-23 38.58858162581914 40.0 38.0 41.0 34.0 41.0 24-25 38.25791164726727 40.0 38.0 41.0 33.0 41.0 26-27 38.13251638281098 40.0 37.0 41.0 33.0 41.0 28-29 38.09372324282166 40.0 37.0 41.0 33.0 41.0 30-31 37.962898125619915 40.0 37.0 41.0 33.0 41.0 32-33 37.63675938249731 40.0 36.0 41.0 33.0 41.0 34-35 37.43962732813944 40.0 35.0 41.0 33.0 41.0 36-37 37.375761917276336 40.0 35.0 41.0 33.0 41.0 38-39 37.00097067625192 39.5 35.0 41.0 31.5 41.0 40-41 36.72024601768411 39.0 35.0 41.0 31.0 41.0 42-43 36.43144228079248 39.0 35.0 41.0 31.0 41.0 44-45 35.960804177722764 38.5 35.0 41.0 30.5 41.0 46-47 35.39976602039692 38.0 35.0 40.5 29.5 41.0 48-49 34.7072796480133 37.0 35.0 40.0 27.5 41.0 50-51 33.95906628574335 36.5 34.0 39.0 26.0 41.0 52-53 33.43646945125001 36.0 34.0 39.0 24.0 41.0 54-55 32.97190973134733 35.0 34.0 38.5 23.0 40.5 56-57 32.58755160691426 35.0 33.5 37.0 23.0 40.0 58-59 32.23965106520062 35.0 33.0 36.5 22.0 39.0 60-61 31.92494426029383 35.0 33.0 36.0 21.5 39.0 62-63 31.63814969608084 35.0 33.0 35.5 20.5 37.5 64-65 31.38334929933282 35.0 33.0 35.0 20.0 37.0 66-67 31.03583024610246 35.0 33.0 35.0 15.5 36.0 68-69 30.636852635237666 35.0 33.0 35.0 4.5 36.0 70-71 30.277346366110258 35.0 32.5 35.0 2.0 35.5 72-73 30.041811137768207 35.0 32.0 35.0 2.0 35.0 74-75 29.948435473342435 35.0 32.0 35.0 2.0 35.0 76-77 29.82588865622801 35.0 32.0 35.0 2.0 35.0 78-79 29.88285760306547 35.0 32.0 35.0 2.0 35.0 80-81 29.878707856119497 35.0 32.0 35.0 2.0 35.0 82-83 29.812807840012205 35.0 32.0 35.0 2.0 35.0 84-85 29.772781220593597 35.0 32.0 35.0 2.0 35.0 86-87 29.72283166184861 35.0 32.0 35.0 2.0 35.0 88-89 29.64046829830704 35.0 32.0 35.0 2.0 35.0 90-91 29.530268991768324 35.0 31.5 35.0 2.0 35.0 92-93 29.451890063496638 35.0 31.0 35.0 2.0 35.0 94-95 29.42888630795446 35.0 31.0 35.0 2.0 35.0 96-97 29.376685967158082 35.0 31.0 35.0 2.0 35.0 98-99 29.27870276960639 35.0 31.0 35.0 2.0 35.0 100-101 29.178057630193543 35.0 31.0 35.0 2.0 35.0 102-103 28.404271823260625 33.5 29.5 35.0 2.0 35.0 104-105 28.89666324739952 34.5 30.5 35.0 2.0 35.0 106-107 28.857425037513035 35.0 31.0 35.0 2.0 35.0 108-109 28.60631660153104 35.0 30.5 35.0 2.0 35.0 110-111 28.286052781051044 35.0 30.0 35.0 2.0 35.0 112-113 27.87327376461313 35.0 29.0 35.0 2.0 35.0 114-115 27.357403843708404 34.0 28.0 35.0 2.0 35.0 116-117 26.84356005052603 34.0 26.0 35.0 2.0 35.0 118-119 26.315228172500618 34.0 24.0 35.0 2.0 35.0 120-121 25.661827414610162 34.0 13.0 35.0 2.0 35.0 122-123 24.960613433481125 34.0 2.0 35.0 2.0 35.0 124-125 22.64760637170542 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1844679064810535 1101 2 0.29564912014511435 1101 3 0.095722760609398 1101 4 0.3854737461261095 1101 5 0.40100841097949314 1101 6 0.4177792483971743 1101 7 0.40041401748160865 1101 8 0.4381792082977256 1101 9 0.45249355700686067 1101 10-11 0.4063987347496223 1101 12-13 0.33797834166121987 1101 14-15 0.7789467179378775 1101 16-17 0.5548059442780158 1101 18-19 0.6495952495094173 1101 20-21 0.5974358723339463 1101 22-23 0.5628636423587849 1101 24-25 0.5044703665202235 1101 26-27 0.49659767275057476 1101 28-29 0.6276619497273614 1101 30-31 0.6850873091176481 1101 32-33 0.8448905675603484 1101 34-35 0.963629287525599 1101 36-37 0.7867432046040861 1101 38-39 0.8850989200724513 1101 40-41 0.8581255297780146 1101 42-43 0.681235722369415 1101 44-45 0.8770038075877693 1101 46-47 1.0027833495095635 1101 48-49 0.8557789782582148 1101 50-51 0.8475590494711298 1101 52-53 0.8595879209550716 1101 54-55 0.7137749614467452 1101 56-57 0.4775864357940307 1101 58-59 0.47979319014785915 1101 60-61 0.44351093551594545 1101 62-63 0.4991281598461903 1101 64-65 0.6107144506378681 1101 66-67 0.19020538265804987 1101 68-69 0.0889618099726377 1101 70-71 0.07674914268061173 1101 72-73 0.31057913217847855 1101 74-75 0.06006481677616904 1101 76-77 0.048679608321563705 1101 78-79 0.0655431792167569 1101 80-81 0.1449538447231724 1101 82-83 0.049296911637149776 1101 84-85 0.09079912698613057 1101 86-87 0.0729047866730923 1101 88-89 -0.03605093234111578 1101 90-91 -0.001734794531369488 1101 92-93 0.15304358851017952 1101 94-95 -0.009035371033554895 1101 96-97 0.07702887210328058 1101 98-99 0.006459372949372266 1101 100-101 0.2165301891591902 1101 102-103 0.006830216526402921 1101 104-105 -0.32700864248254646 1101 106-107 -0.2463554792023821 1101 108-109 -0.7133027587165621 1101 110-111 -0.16086962720357434 1101 112-113 -0.03505583083113706 1101 114-115 0.035536974733734183 1101 116-117 -0.06746492231472345 1101 118-119 -0.1588737427818856 1101 120-121 -0.17181112963259437 1101 122-123 0.020980080976347182 1101 124-125 -0.40052538039256547 1102 1 -0.08503966927651874 1102 2 -0.07158572833973409 1102 3 0.17697276060940226 1102 4 0.11085253400489137 1102 5 0.13718265340373392 1102 6 0.1509231877911148 1102 7 0.38355795687554206 1102 8 -0.1989420038234826 1102 9 0.15325113276443147 1102 10-11 0.3066828256587115 1102 12-13 0.3540768265096972 1102 14-15 0.4090603543015092 1102 16-17 0.10736276245983589 1102 18-19 0.14211418890334926 1102 20-21 0.05112905415212765 1102 22-23 0.3942083393284861 1102 24-25 0.4766294574293113 1102 26-27 0.287601460629368 1102 28-29 0.49518088912130054 1102 30-31 0.3897274606327983 1102 32-33 0.5480155675603484 1102 34-35 0.5637239844953044 1102 36-37 0.6401522955131753 1102 38-39 0.8040383140118479 1102 40-41 0.5716671964446789 1102 42-43 0.6003645102481983 1102 44-45 0.6887462318301942 1102 46-47 1.2452075919338057 1102 48-49 1.7597562509854896 1102 50-51 1.9944340494711312 1102 52-53 2.181841708833865 1102 54-55 1.8838507190224973 1102 56-57 1.5420750721576653 1102 58-59 1.7820659174205815 1102 60-61 1.7777912385462429 1102 62-63 1.5067986143916379 1102 64-65 1.5591046021530168 1102 66-67 1.653084170536836 1102 68-69 1.9162345372453657 1102 70-71 2.0315786881351556 1102 72-73 2.247132162481506 1102 74-75 1.8920723925337413 1102 76-77 2.1144940022609546 1102 78-79 2.126054542853119 1102 80-81 2.1327379356322673 1102 82-83 2.1424787298189685 1102 84-85 2.2020680663800682 1102 86-87 1.9804805442488487 1102 88-89 1.8591195222043382 1102 90-91 2.024212175165598 1102 92-93 2.148782224873816 1102 94-95 2.0564949319967454 1102 96-97 2.2326159933154024 1102 98-99 2.009110888100885 1102 100-101 2.0922877649167653 1102 102-103 2.1085347619809482 1102 104-105 2.518919387820482 1102 106-107 2.127697551100649 1102 108-109 2.1858449685561645 1102 110-111 2.349925827341881 1102 112-113 2.2800956843203792 1102 114-115 2.0492680353397965 1102 116-117 2.2541259867761845 1102 118-119 2.3240808026726576 1102 120-121 1.7372797794583121 1102 122-123 1.7909611415824074 1102 124-125 1.5163306802134962 1103 1 -0.31509604700731586 1103 2 -0.080077624040932 1103 3 -0.04512026264641378 1103 4 0.05902025775401398 1103 5 0.013363062142282445 1103 6 0.22083157397857178 1103 7 0.18369890120253274 1103 8 0.07131874318145037 1103 9 0.07516797561150668 1103 10-11 0.2587969905635745 1103 12-13 0.1523678765449361 1103 14-15 -0.03137304950398345 1103 16-17 8.088512547601567E-4 1103 18-19 -0.053675099327790576 1103 20-21 0.1733806397758002 1103 22-23 0.2664246888704156 1103 24-25 0.12874362233417713 1103 26-27 0.004373835541272797 1103 28-29 0.13892648461108337 1103 30-31 0.3184448672571847 1103 32-33 0.587550451281281 1103 34-35 0.5493124270604923 1103 36-37 0.29459204181338094 1103 38-39 0.6467268270491928 1103 40-41 0.3863958786152182 1103 42-43 0.34213688516011587 1103 44-45 0.5150852029366035 1103 46-47 -0.1879143249090447 1103 48-49 -0.5676221845324747 1103 50-51 0.04966660761066777 1103 52-53 -0.11068824183561787 1103 54-55 0.12104240330720728 1103 56-57 -0.0256839130431743 1103 58-59 0.18967691107808804 1103 60-61 0.13755163319036967 1103 62-63 0.3685322296136313 1103 64-65 0.030845264591359722 1103 66-67 0.15881003382083847 1103 68-69 0.47791529834473323 1103 70-71 0.6628683287271215 1103 72-73 0.4081082019459181 1103 74-75 0.63259388654361 1103 76-77 0.6321825152983074 1103 78-79 0.39381352805396475 1103 80-81 0.34706140286271037 1103 82-83 0.23766900466040752 1103 84-85 -0.10186075673480133 1103 86-87 0.31592804248704454 1103 88-89 0.2580624397519067 1103 90-91 0.23649194965467402 1103 92-93 0.37869765827762336 1103 94-95 0.2889297452455146 1103 96-97 -0.17929380231532122 1103 98-99 -0.20138946425992899 1103 100-101 -0.6892837643291791 1103 102-103 -0.12296629510150581 1103 104-105 -0.10033713085464058 1103 106-107 -0.26888454896982594 1103 108-109 -0.1501486889491197 1103 110-111 0.4918920007034018 1103 112-113 0.6288976575409571 1103 114-115 1.1962201142686197 1103 116-117 1.3805001939643446 1103 118-119 1.649556489776252 1103 120-121 1.4648167773441507 1103 122-123 1.0448899646972762 1103 124-125 0.896422294026042 1104 1 0.1500202320624524 1104 2 0.1524805154939486 1104 3 0.3095309001442814 1104 4 0.3032063042656432 1104 5 0.23429329470042148 1104 6 0.07548273676926698 1104 7 0.31160587794672523 1104 8 0.34457455713494056 1104 9 0.20888890584406994 1104 10-11 0.3634481533542697 1104 12-13 0.3442283416612142 1104 14-15 0.5442083458448579 1104 16-17 0.48046001404546246 1104 18-19 0.39690629602104366 1104 20-21 0.5134969188455756 1104 22-23 0.4088665493355279 1104 24-25 0.6083947851248723 1104 26-27 0.3764668587970945 1104 28-29 0.34822881019247376 1104 30-31 0.44635184400137007 1104 32-33 0.7765039396533737 1104 34-35 0.8516380084558364 1104 36-37 1.0823827394878052 1104 38-39 0.8124245014678024 1104 40-41 0.5753493669873109 1104 42-43 0.4409740944624332 1104 44-45 0.5615968308435839 1104 46-47 0.8469693960211941 1104 48-49 1.0166801410489228 1104 50-51 1.19210846807578 1104 52-53 1.3050094325829846 1104 54-55 1.3594144963304657 1104 56-57 1.0440835288172892 1104 58-59 0.843746678519949 1104 60-61 0.8497609355159454 1104 62-63 0.6417880435671144 1104 64-65 1.0046824738936841 1104 66-67 0.7779960803324641 1104 68-69 0.6726827402051967 1104 70-71 0.5960078636108435 1104 72-73 0.690084946131968 1104 74-75 0.6994543516598881 1104 76-77 0.46067088739133055 1104 78-79 0.6321856210772197 1104 80-81 0.315084658676664 1104 82-83 0.8045294697766892 1104 84-85 0.8923252897768279 1104 86-87 0.981625716905647 1104 88-89 0.9586438351007445 1104 90-91 0.9225384612825813 1104 92-93 0.7188139373473916 1104 94-95 0.7133483498966768 1104 96-97 0.7015201511730496 1104 98-99 0.6009361171354186 1104 100-101 0.7060650728801221 1104 102-103 0.6444755653636101 1104 104-105 0.5421047296104753 1104 106-107 0.3386735905650582 1104 108-109 0.2161303808183206 1104 110-111 0.570380372796425 1104 112-113 0.8963395180060729 1104 114-115 0.2369177886872258 1104 116-117 -0.19798817812867853 1104 118-119 -0.05102490557258221 1104 120-121 -0.08460182730701149 1104 122-123 0.1379132205112299 1104 124-125 0.27142229402603846 1105 1 0.334396067400597 1105 2 0.26655429255890795 1105 3 0.4275474732530782 1105 4 0.4103300679651838 1105 5 0.313149215577198 1105 6 0.19543729437418733 1105 7 0.19358930483792847 1105 8 0.13386886347014126 1105 9 0.30852803976547705 1105 10-11 0.3841645393473243 1105 12-13 0.5698030543048915 1105 14-15 0.6600530397769617 1105 16-17 0.8196407143929605 1105 18-19 0.8165133529576849 1105 20-21 0.8152878838281978 1105 22-23 0.7995374929334957 1105 24-25 0.9207778377845912 1105 26-27 0.7589180750494293 1105 28-29 0.8753271796124196 1105 30-31 0.994534148198106 1105 32-33 1.0342224641120765 1105 34-35 0.667329000169282 1105 36-37 0.6722317103512125 1105 38-39 0.38653570168165174 1105 40-41 0.6268396102377807 1105 42-43 0.5636351476567683 1105 44-45 0.8346187501165048 1105 46-47 0.8297948437624427 1105 48-49 1.0657646104421303 1105 50-51 0.9455834747584859 1105 52-53 1.037066369230942 1105 54-55 1.291217490182376 1105 56-57 1.302837872575644 1105 58-59 1.2754828453202691 1105 60-61 1.046600015975713 1105 62-63 1.2350117805358458 1105 64-65 1.2748308299482183 1105 66-67 0.8623318194396568 1105 68-69 1.323444568593331 1105 70-71 0.872905751876015 1105 72-73 0.6984383275807744 1105 74-75 0.8553952765462824 1105 76-77 0.7067615048732847 1105 78-79 0.5308089838144596 1105 80-81 0.7286104539185772 1105 82-83 0.7047566817520945 1105 84-85 0.8120994143424518 1105 86-87 0.6884220280524005 1105 88-89 1.0574476308772773 1105 90-91 0.9918715273077083 1105 92-93 1.1819588183952376 1105 94-95 1.133924399081387 1105 96-97 0.8211739870458103 1105 98-99 0.5761432809953497 1105 100-101 0.7676796144465463 1105 102-103 0.8906664234229531 1105 104-105 0.893214058666878 1105 106-107 0.7985439460849726 1105 108-109 0.8011009769156203 1105 110-111 0.8246907176240121 1105 112-113 0.7862085369849545 1105 114-115 0.5886619747337356 1105 116-117 0.6048051926278042 1105 118-119 0.7677067169882292 1105 120-121 1.1672693301375219 1105 122-123 1.329205655688991 1105 124-125 1.395954504664907 1106 1 -0.16011082280071065 1106 2 -0.2684047472582023 1106 3 -0.19650790237402305 1106 4 -0.202993104702621 1106 5 -0.10966147852326458 1106 6 -0.24261439801166773 1106 7 0.2099098738351941 1106 8 0.17340020277286783 1106 9 -0.20261694023071186 1106 10-11 -0.45439201110673366 1106 12-13 -0.6440313268470703 1106 14-15 -0.491944166040021 1106 16-17 -0.4528080059982216 1106 18-19 -0.20225212618119315 1106 20-21 -0.44615874092572483 1106 22-23 -0.6276232360942515 1106 24-25 -0.327539301988061 1106 26-27 -0.6500010841554982 1106 28-29 -0.2541578016538537 1106 30-31 -0.839688243368542 1106 32-33 -0.2812662003954429 1106 34-35 -0.6100412925848886 1106 36-37 -0.533077237384866 1106 38-39 -0.5285751130767196 1106 40-41 -0.4684801332054107 1106 42-43 -0.5007739461388709 1106 44-45 0.03964192913473141 1106 46-47 0.12212036608414678 1106 48-49 -0.5334130106920583 1106 50-51 -0.3121957847830146 1106 52-53 0.11274745134181785 1106 54-55 -0.2505206186637494 1106 56-57 -0.18876370232751327 1106 58-59 -0.6131626109571151 1106 60-61 -0.9368412744288079 1106 62-63 -0.8416694920875152 1106 64-65 -0.859813864279257 1106 66-67 -0.34750179966239614 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-2.006534843147442 2216 66-67 -2.1178454647995792 2216 68-69 -2.374879997936965 2216 70-71 -2.2517289646640215 2216 72-73 -1.9475776474825395 2216 74-75 -1.7379224713594255 2216 76-77 -2.1647561261417145 2216 78-79 -2.186169391404711 2216 80-81 -2.42144799143502 2216 82-83 -2.446324557289401 2216 84-85 -2.359924743070369 2216 86-87 -2.573705382818435 2216 88-89 -2.8536180227365975 2216 90-91 -2.553959370802559 2216 92-93 -2.8576379086649624 2216 94-95 -2.5567048625589806 2216 96-97 -2.360347540326096 2216 98-99 -2.5602779151862194 2216 100-101 -2.5507014492588844 2216 102-103 -2.5216655461854636 2216 104-105 -2.4396322300531708 2216 106-107 -3.422379490501818 2216 108-109 -3.6131791711459407 2216 110-111 -3.9458625650566823 2216 112-113 -2.7768637404356546 2216 114-115 -2.9339368953227627 2216 116-117 -2.9679592725972093 2216 118-119 -2.7253285450417764 2216 120-121 -3.3017546324574525 2216 122-123 -2.958345483995405 2216 124-125 -3.1019024990366333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 9.0 5 33.0 6 81.0 7 157.0 8 228.0 9 309.0 10 431.0 11 800.0 12 1154.0 13 1232.0 14 1690.0 15 2285.0 16 1167.0 17 500.0 18 496.0 19 648.0 20 981.0 21 1330.0 22 1277.0 23 575.0 24 715.0 25 863.0 26 1171.0 27 1425.0 28 1735.0 29 2106.0 30 2813.0 31 3629.0 32 4967.0 33 6834.0 34 10001.0 35 16079.0 36 36784.0 37 12615.0 38 831.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.77437988447162 20.36697247706422 12.568807339449542 14.28984029901461 2 21.25738604091252 21.881331649132328 36.138827897828904 20.722454412126247 3 28.723539534923155 22.25519036275316 29.583160250595547 19.438109851728143 4 16.349638834424045 24.202762004193083 26.061640056700025 33.38595910468285 5 15.007675150322697 34.545809198307225 27.638173907881235 22.808341743488842 6 29.937180497978076 36.07416261858135 9.391557940605475 24.597098942835103 7 26.909349858849264 41.362676862299615 12.079620885222832 19.648352393628294 8 36.79668359345196 27.256928254732575 11.513322425588552 24.43306572622691 9 34.040641239752794 14.239693452809874 16.65663493247654 35.06303037496079 10-11 30.903958154952143 25.894166617214452 16.137386719114268 27.06448850871913 12-13 34.5268271178969 26.711823599725328 18.105019540687866 20.65632974168991 14-15 36.92554192558431 20.40115633398045 22.64558024398308 20.02772149645216 16-17 31.68261853694928 21.405742673301738 23.82819454217143 23.083444247577546 18-19 29.914207478869777 21.65625344399325 24.307174526742344 24.12236455039463 20-21 38.53033681194313 18.28940564094304 22.064022245017338 21.11623530209649 22-23 33.52647953950101 22.295034715451976 21.853355827024647 22.325129918022363 24-25 27.634601853186275 23.763765376105255 23.982061563763683 24.619571206944784 26-27 24.073618799752456 23.935011317491668 26.596529302554277 25.394840580201596 28-29 24.76199357403844 21.49772378538306 28.93081494417552 24.809467696402987 30-31 27.562119041361825 21.97119338075094 25.47156215295145 24.995125424935782 32-33 23.669664883561236 22.06741325375766 26.96233437041684 27.30058749226426 34-35 23.424240625980215 22.336998448613503 27.997015912308516 26.241745013097773 36-37 24.408480912859552 23.215693588450222 25.68010919048144 26.695716308208784 38-39 25.714867030069772 19.113844640934563 27.36162564959011 27.809662679405555 40-41 23.822684152968403 19.57163082087844 28.529828160632082 28.07585686552107 42-43 24.672131842419823 18.794665943251466 30.13843793182377 26.39476428250494 44-45 25.675446553463495 19.007027865614322 29.50431929738299 25.8132062835392 46-47 23.375918751430582 18.859942861502727 28.38571028916827 29.378428097898425 48-49 22.595138988970746 19.560186166379843 29.722191608948872 28.12248323570054 50-51 23.27376461312829 19.702608533473494 28.66716401461525 28.356462838782964 52-53 22.85667053806831 19.348248120109528 28.150035181715683 29.645046160106475 54-55 20.993735111352247 20.65844912215261 26.069227443433736 32.2785883230614 56-57 21.866919861985945 21.515950457362305 25.826346442407953 30.790783238243797 58-59 22.01273323781992 20.21761798591036 26.554141693300217 31.215507082969506 60-61 21.435837875872124 21.5002670419383 25.19138005578209 31.872515026407484 62-63 20.139624784882884 22.70026025992082 24.899753304114142 32.26036165108216 64-65 19.71913970108258 22.40397087123492 24.903144312854465 32.973745114828034 66-67 20.674132537576615 23.581498656312785 23.9947778465399 31.7495909595707 68-69 20.02941700082232 23.762493747827634 23.52257987944964 32.68550937190041 70-71 19.61825719105791 23.258081197704286 24.655600674810742 32.468060936427065 72-73 20.235420781797064 21.814783102603446 25.232072160665993 32.71772395493349 74-75 20.37233275968769 21.6359073915513 24.525470714400765 33.466289134360245 76-77 20.627506167397147 21.82241287226918 24.461889300519672 33.088191659814 78-79 20.788579082562585 22.932968234725625 24.984528522622266 31.29392416008952 80-81 20.357920972541308 24.16220890309345 24.032502818776017 31.44736730558923 82-83 19.472867691316477 23.680685661967292 24.75648318483541 32.08996346188082 84-85 19.875549979230072 22.64727574835324 25.424511906679438 32.05266236573725 86-87 20.324010885138055 23.264863215184935 24.837443518510668 31.573682381166336 88-89 19.922600225502084 23.728159784331844 24.6144846938343 31.734755296331773 90-91 19.75940792987394 23.102942547834417 24.781067998202765 32.35658152408888 92-93 19.810273060978815 23.01053755966056 24.835324138047966 32.343865241312656 94-95 19.508473283089884 23.13642875914513 24.29191498741088 33.063182970354106 96-97 19.58392322756212 23.631939911325123 23.803185852711536 32.98095100840122 98-99 19.78144948668605 23.9133936367721 24.102442374045218 32.20271450249663 100-101 20.172687120101052 24.09142159563916 23.55648996685289 32.1794013174069 102-103 20.25831009079426 24.719605964784375 22.83759611390398 32.184487830517384 104-105 20.223552251205927 26.098051017726497 22.603616510821556 31.07478022024602 106-107 20.289253045549724 27.71089954984359 22.26197238023381 29.737875024372872 108-109 19.957781941182954 28.50270009070948 21.755864325740298 29.783653642367263 110-111 19.611475173577258 29.804423570901754 21.3396180028654 29.244483252655584 112-113 19.900558668689968 30.857755660865216 21.309946676387558 27.93173899405726 114-115 19.492448615402875 31.460640818592978 21.16676627797064 27.880144288033502 116-117 19.11489794620716 32.20194569848032 21.22210211737776 27.46105423793476 118-119 19.540512472712628 32.739757963673355 21.27503550007418 26.444694063539835 120-121 19.573750201341145 33.17805339143261 21.257809917005062 25.99038649022118 122-123 18.91571116715766 33.8524468558591 21.328444566899098 25.90339741008414 124-125 18.21820463680613 35.413310041890675 21.347285585875888 25.021199735427302 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 1.5 9 3.5 10 4.0 11 3.5 12 3.5 13 3.0 14 7.5 15 9.0 16 6.0 17 8.0 18 14.5 19 18.0 20 23.5 21 32.5 22 53.0 23 73.0 24 96.0 25 130.0 26 179.0 27 226.0 28 290.5 29 386.0 30 478.0 31 545.0 32 609.0 33 692.5 34 747.5 35 725.0 36 682.0 37 659.0 38 625.0 39 619.5 40 587.0 41 583.0 42 637.5 43 667.0 44 695.0 45 794.0 46 983.5 47 1170.0 48 1567.0 49 2480.0 50 4083.5 51 6605.5 52 9936.5 53 12542.5 54 12877.5 55 11830.0 56 10190.0 57 8045.5 58 5913.5 59 4190.5 60 2988.0 61 2247.5 62 1807.0 63 1353.5 64 1087.5 65 962.5 66 823.5 67 651.0 68 426.5 69 288.0 70 220.0 71 183.5 72 145.0 73 113.0 74 94.0 75 76.5 76 58.0 77 39.0 78 22.5 79 11.5 80 7.0 81 7.0 82 5.0 83 1.5 84 1.5 85 1.0 86 1.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20261277223442042 2 0.0 3 0.0 4 0.12377181902186354 5 0.03899660051373783 6 0.001695504370162514 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.0 114-115 4.238760925406285E-4 116-117 0.005510389203028171 118-119 0.0012716282776218857 120-121 0.0 122-123 0.0012716282776218857 124-125 0.02797582210768148 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 117959.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.85473766308633 #Duplication Level Percentage of deduplicated Percentage of total 1 82.96645839324462 53.80767893929247 2 9.186687929727327 11.916004713502149 3 3.0482863193119134 5.930874286828474 4 1.6156440354500539 4.191286803041735 5 0.9071658257300462 2.941700082231962 6 0.5921413819246556 2.3041904390508563 7 0.4065253195994876 1.8455565069218967 8 0.279731248856239 1.4513517408591121 9 0.22090925727431965 1.289431073508592 >10 0.7280855402473139 7.221153112522148 >50 0.019607330527306475 0.8350359023050382 >100 0.02222164126428067 2.9043989860883865 >500 0.005228621473948394 2.1363355064047678 >1k 0.0013071553684870984 1.2250019074424163 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 1445 1.2250019074424163 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 813 0.689222526471062 RNA PCR Primer, Index 17 (95% over 21bp) ACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCCCGCA 583 0.49423952390237286 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 575 0.4874575064217228 RNA PCR Primer, Index 17 (95% over 23bp) GGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTT 549 0.46541594960961014 No Hit GTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTT 444 0.3764019701760781 No Hit TATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTC 388 0.32892784781152773 No Hit GGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAA 336 0.2848447341873024 No Hit ATTCACGCATTAACCGTTCATCCTTTCCCGCATGCGCGTCGATATCTACC 298 0.2526301511542146 No Hit ACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTCCCGCA 180 0.15259539331462627 No Hit GTGGTATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCC 174 0.14750888020413874 No Hit GGGGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGA 172 0.14581337583397622 No Hit GGTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTT 169 0.14327011927873246 No Hit GTATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTT 156 0.13224934087267612 No Hit ATCAACGCAGAGTCGCCGGGCAATTCACGCATTAACCGTTCATCCTTTCC 148 0.12546732339202604 No Hit TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC 148 0.12546732339202604 No Hit CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG 144 0.122076314651701 TruSeq Adapter, Index 12 (95% over 23bp) TATCAACGCAGAGTCGCCGGACAATTCACGCATTAACCGTTCATCCTTTC 143 0.12122856246661974 No Hit ATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAATTGCCC 139 0.11783755372629472 No Hit GGTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAAT 137 0.1161420493561322 No Hit GTAGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAATT 129 0.10936003187548216 No Hit AGATATCGACGCGCATGCGGGAAAGGATGAACGGTTAATGCGTGAATTGC 121 0.10257801439483209 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 8.477521850812571E-4 0.0 0.0 0.0 0.0 9 8.477521850812571E-4 0.0 0.0 0.0 0.0 10-11 0.0016955043701625142 0.0 0.0 0.0 0.0 12-13 0.0016955043701625142 0.0 0.0 0.0 0.0 14-15 0.0016955043701625142 0.0 0.0 0.0 0.0 16-17 0.002119380462703143 0.0 0.0 8.477521850812571E-4 0.0 18-19 0.0025432565552437713 0.0 0.0 0.0025432565552437713 0.0 20-21 0.0025432565552437713 0.0 0.0 0.005510389203028171 0.0 22-23 0.0029671326477844 0.0 0.0 0.015683415424003255 0.0 24-25 0.0033910087403250283 0.0 0.0 0.03094295475546588 0.0 26-27 0.0033910087403250283 0.0 0.0 0.07926482930509754 0.0 28-29 0.0033910087403250283 0.0 0.0 0.2450003814884833 0.0 30-31 0.0033910087403250283 0.0 0.0 0.5662984596342797 0.0 32-33 0.0033910087403250283 0.0 0.0 1.0791885316084402 0.0 34-35 0.004662637017946914 0.0 0.0 1.8777710899549844 0.0 36-37 0.005086513110487543 0.0 0.0 2.984511567578566 0.0 38-39 0.005086513110487543 0.0 0.0 4.361684992243068 0.0 40-41 0.005086513110487543 0.0 0.0 5.919853508422419 0.0 42-43 0.005086513110487543 0.0 0.0 7.462762485270305 0.0 44-45 0.005086513110487543 0.0 0.0 8.934460278571368 0.0 46-47 0.005086513110487543 0.0 0.0 10.671928381895405 0.0 48-49 0.005086513110487543 0.0 0.0 12.750192863622107 0.0 50-51 0.005086513110487543 0.0 0.0 14.583033087767785 0.0 52-53 0.005086513110487543 0.0 0.0 16.197153248162497 0.0 54-55 0.005086513110487543 0.0 0.0 18.110106053798354 0.0 56-57 0.005086513110487543 0.0 0.0 20.229486516501495 0.0 58-59 0.005086513110487543 0.0 0.0 22.159394365838978 0.0 60-61 0.005086513110487543 0.0 0.0 23.987148076874167 0.0 62-63 0.005086513110487543 0.0 0.0 25.664425775057435 0.0 64-65 0.005086513110487543 0.0 0.0 27.451487381208725 0.0 66-67 0.005086513110487543 0.0 0.0 29.461931688128928 0.0 68-69 0.005086513110487543 0.0 0.0 31.58851804440526 0.0 70-71 0.005086513110487543 0.0 0.0 33.836756839240756 0.0 72-73 0.005086513110487543 0.0 0.0 36.07694198831797 0.0 74-75 0.005086513110487543 0.0 0.0 38.14418569163862 0.0 76-77 0.005086513110487543 0.0 0.0 40.33308183351842 0.0 78-79 0.005086513110487543 0.0 0.0 42.53299875380429 0.0 80-81 0.005510389203028172 0.0 0.0 44.702820471519765 0.0 82-83 0.0059342652955688 0.0 0.0 47.00234827355268 0.0 84-85 0.0059342652955688 0.0 0.0 49.58502530540272 0.0 86-87 0.0059342652955688 0.0 0.0 51.70525352029095 0.0 88-89 0.0059342652955688 0.0 0.0 53.561406929526356 0.0 90-91 0.0059342652955688 0.0 0.0 55.95630685238091 0.0 92-93 0.006782017480650057 0.0 0.0 57.994726981408796 0.0 94-95 0.006782017480650057 0.0 0.0 59.75423664154495 0.0 96-97 0.006782017480650057 0.0 0.0 61.514594053866176 0.0 98-99 0.006782017480650057 0.0 0.0 63.05750303071406 0.0 100-101 0.006782017480650057 0.0 0.0 64.50122500190744 0.0 102-103 0.006782017480650057 0.0 0.0 65.79574258852652 0.0 104-105 0.006782017480650057 0.0 0.0 67.00506108054493 0.0 106-107 0.006782017480650057 0.0 0.0 68.0948465144669 0.0 108-109 0.006782017480650057 0.0 0.0 69.1507218609856 0.0 110-111 0.006782017480650057 0.0 0.0 70.21677023372527 0.0 112-113 0.006782017480650057 0.0 0.0 71.29172000440832 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTTGG 20 8.3785283E-4 89.47439 1 CTTTACA 30 0.0041852943 59.649597 1 TACACGG 40 1.762047E-4 59.523113 2 TAGAGCT 15 0.0042329812 59.497883 54-55 TCCATAG 15 0.0042329812 59.497883 52-53 CTCCGCA 15 0.0042329812 59.497883 90-91 ATGTGTA 30 0.004227741 59.497883 8 AAGAATC 15 0.0042329812 59.497883 70-71 GTAGGAG 30 0.004227741 59.497883 8 CCATAGG 15 0.0042329812 59.497883 54-55 GTCCATA 15 0.0042329812 59.497883 52-53 GGGGCGC 30 0.004227741 59.497883 6 GAGGTTT 20 1.7693937E-4 59.49788 14-15 GTACACG 45 3.120299E-4 53.021862 1 AGTCCGT 35 0.0077801165 50.998184 6 GTCCGTG 35 0.0077801165 50.998184 7 TACACGT 35 0.0077801165 50.998184 5 ACACGGG 50 5.302871E-4 47.618492 3 TCTACAC 50 5.302871E-4 47.618492 3 AGTATGG 65 3.501625E-5 45.7676 7 >>END_MODULE