FastQCFastQC Report
Thu 26 May 2016
SRR935907_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935907_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685840
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC122640.45661692431418105No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT107530.4003589193697316No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT99280.3696422720638608No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG97480.3629404581062163No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT97350.3624564382092753No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT81750.304374050576356No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA56500.21036249367050902No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC38320.14267417269829924No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC34910.12997795847853932No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA34800.1295684031811277No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC32350.12044648973877818No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG31670.11791469335477914No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC30550.11374467578113365No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG30420.11326065588419265No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT28500.10611205432937182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGTA20450.064.8775252
GAGTAGT26700.062.4758341
GGTATCA58050.061.1658971
GATTGTG86350.056.7674069
GAGTACG10450.054.7293471
AGGGTAA71900.054.027956
GAGTAAG20500.052.891131
GTATCAA97750.051.195031
AGTACGG7700.050.9959752
GATAGCG20350.050.2803429
TAGTAGG43900.049.7375342
GTAGTAG44600.049.69051
ACGGGAT10950.049.4389885
GGGATTG100150.049.4204377
GAGTCAG73300.048.928041
GAGTTAG20850.048.574481
TATAACG909.7366865E-846.2741282
GTGGGGT81300.046.098237
TCGTAGG13050.045.5902752
GGGGTAA95500.045.410729
Produced by FastQC (version 0.11.5)