FastQCFastQC Report
Thu 26 May 2016
SRR935907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685840
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT112240.4178953325589015No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG103890.3868063622553838No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT99900.37195067464927173No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC99470.37034968575939No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT97940.3646531438953921No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT88690.33021326661305217No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA45330.16877401483334822No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC35630.13265868406159712No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG34260.12755785899383432No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG33900.12621749620230543No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA33710.12551008250677625No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC32570.12126560033360141No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC32350.12044648973877818No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC29390.1094257290084294No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT28450.10592589283054835No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT27800.10350579334584338No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG27110.10093676466207965No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGTG84300.063.5181359
GGTATCA64350.062.534081
AGGGTAA76300.060.4332736
GAGTAGT24750.058.3775181
GATAGCG18800.056.96369
GTAGTAG45900.056.0621871
TAGTAGG46050.055.633522
AGTAGTA19600.055.6279072
GGGATTG99450.054.021397
GAGTAAG18900.051.490991
GTATCAA109300.050.418161
GAGTACG11100.050.0225031
TAGGGTA76550.048.6695675
TCGTAGG13250.047.663692
AGTAGGG53950.047.2652443
GTAGGGT75600.046.9692964
GAGTCAG68950.046.0662421
ACGGGAT7100.044.4268845
AGGGGAT25750.043.9141735
GAGTCGG12950.043.79851
Produced by FastQC (version 0.11.5)