FastQCFastQC Report
Thu 26 May 2016
SRR935906_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935906_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369524
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC155401.1347008157578837No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG113560.8291932087352978No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT89110.6506640263332369No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG79680.5818079858403358No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT79520.5806396967121423No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT74100.5410639024945894No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA68600.5009039637129397No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC49990.36501733448993956No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT49880.36421413571430655No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT48170.35172804565673915No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC46650.34062929893890137No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC44220.3228859078044634No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC43990.3212064921826854No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT43790.31974613077244357No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA40690.29711052891369555No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG36810.2687795175550045No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC36370.26556672245247254No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG33600.24534071692062354No Hit
GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG32900.2402294519847772No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG31050.2267211089400405No Hit
GAGTAGGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCAC31030.22657507279901629No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT30600.22343529576699644No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA30190.2204415548760007No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC28330.20686019376075193No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG27850.20335532637617157No Hit
GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG25790.18831360385068097No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG24970.18232612206868956No Hit
GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG24020.17538940537004097No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA22080.16122389969069545No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA20530.14990609876132144No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA18380.13420721360122204No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCCACTGACTCTGCGT17360.12675937040898883No Hit
CACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT17130.12507995478721073No Hit
GTATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCC16600.12120999705006995No Hit
CTAATAGGGAACGTGAGCTGGGATTAGACCGTCGTGAGACAGGTTAGTTT15830.115587605620639No Hit
GTAGGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAG15650.11427328035142137No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCG15040.10981917805018386No Hit
GTATCAACGCAGAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA14600.10660638294765189No Hit
CTAATAGGGAACGTGAGCTGGGGTTAGACCGTCGTGAGACAGGTTAGTTT14560.10631431066560353No Hit
GAGTAAGGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCA13960.10193322643487811No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCA13940.10178719029385393No Hit
GAGTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA13920.10164115415282975No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTA13830.10098399151822093No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCAC13820.10091097344770883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGT1050.090.663532
GAGTTAG18300.074.568071
AGTAGTA24200.072.2826162
CGGGTAG250.002059003671.381886
GAGTAAG14800.070.862991
GAGTAGT31450.070.484051
AGTTAGT13600.069.9975742
AGTACGG6150.069.656122
GAGTACG9450.069.363241
AGTTGTA4550.069.305292
GTATTGG3900.067.228991
GAGTAGG19750.066.981421
GTATCGG3100.065.356051
GTATACG1550.065.356051
AGTCGCG1200.064.45612
GTTAGTG14750.063.7310523
AGTAAGG9750.063.4644662
TATTGGG4150.063.0821532
GAGTAAT5700.062.7256131
GAGTCAG81250.062.339611
Produced by FastQC (version 0.11.5)