FastQCFastQC Report
Thu 26 May 2016
SRR935906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR935906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369524
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC124120.9063002911960651No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG121470.8869505025103613No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT87510.6389811350513025No Hit
GTCTAACCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT79110.5776459558211466No Hit
GTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT75430.5507753058726974No Hit
GAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG64010.46738866934789025No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT56460.412260026111262No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA56080.4094853394318026No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT47940.350048630034961No Hit
GGTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTC47150.3442802024645059No Hit
GTATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCT46930.34267380491323995No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCAC44990.3285082992338944No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTC40190.29345962538809106No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACG39370.28747214360609963No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA39040.28506254727920066No Hit
GTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCACG37480.2736717282793146No Hit
GAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTC37000.27016686089473424No Hit
AAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAG34470.2516932890551754No Hit
TATCAACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTA31400.2292767414079636No Hit
GGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCT29940.21861610311319843No Hit
GTCGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG29340.21423501888247304No Hit
ACGCAGAGTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC28160.20561888656204638No Hit
GAGTTAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTC27250.19897424214544618No Hit
GTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG26760.1953963566903537No Hit
GTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCG26670.1947391940557449No Hit
GAGTTACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG25690.18758342314556006No Hit
GAGTAGGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCAC25150.1836404473379072No Hit
CACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT20670.1509283517484907No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCCAGCTCA18240.13318496061405277No Hit
GAGTAACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCG18150.13252779797944395No Hit
CTAATAGGGAACGTGAGCTGGGATTAGACCGTCGTGAGACAGGTTAGTTT18060.13187063534483512No Hit
GAGTAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCA17850.13033725586408124No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCCACTGACTCTGCGT17820.13011820165254498No Hit
CTAATAGGGAACGTGAGCTGGGGTTAGACCGTCGTGAGACAGGTTAGTTT16690.12186715968467876No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTA16160.11799720194753797No Hit
GTATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCC16020.11697494896036871No Hit
GTAGGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAG15990.11675589474883243No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCG15660.11434629842193346No Hit
GAGTCAGAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA14900.1087969250630146No Hit
GTTGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG14720.10748259979379696No Hit
GGTATCAACGCAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGC14550.10624129259509142No Hit
GTCAGTGGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCAC14350.10478093118484963No Hit
GTGGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACG13880.10134908187078137No Hit
CAGAGTCACTGGGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTT13720.10018079274258793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG1350.084.0319061
GTGTACG2250.074.30191
TGTACGG2350.070.986672
AGTACGT1350.070.611072
AGTTCGT850.070.0918662
TCGTAGG10800.070.0594252
GTACACG2900.070.0011751
AGTTGTA4350.069.850182
AGTCGCG1550.069.187462
TACACGG2950.068.6662752
TATACGG1650.068.605072
AGTCGTA4100.068.296842
GTCGTAG11250.066.871711
GTATTGG3700.066.161681
GAGTTAG16800.065.748661
AGTAGTA21050.064.814182
GTAGTAG46450.064.784221
AAGGGTA2300.064.690215
ATACGGG1750.064.6847843
GTGTAGG4350.064.510891
Produced by FastQC (version 0.11.5)