FastQCFastQC Report
Thu 26 May 2016
SRR1364640_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364640_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4279243
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95500.042.11091
ATCAACG108050.037.5492443
TCAACGC108000.037.2908064
CAACGCA108500.037.119835
AACGCAG112900.034.7438856
TATCAAC120600.033.2959482
GGTATCA57850.032.132561
ACGCAGA131950.029.22687
GTACATG134500.029.0143661
CGCAGAG131100.027.413768
TACATGG141150.026.7600652
ACATGGG137050.026.4303783
CGCATAG4850.024.5370628
GCAGAGT155650.021.8278589
AGAGTAC128800.020.69320710-11
GAGTACA108750.020.3801691
AACGCAT7300.019.56936
GGACCGA10550.019.1828616
GAGTACT67150.019.13731612-13
AGTACAT108000.018.8108962
Produced by FastQC (version 0.11.5)