##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364640_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4279243 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.89405626182014 31.0 26.0 31.0 2.0 34.0 2 25.40233891835542 30.0 25.0 31.0 2.0 34.0 3 26.128711783836533 31.0 26.0 31.0 2.0 34.0 4 29.453824660109277 35.0 28.0 37.0 2.0 37.0 5 29.744926614356793 35.0 30.0 37.0 2.0 37.0 6 29.629674921475598 35.0 30.0 37.0 2.0 37.0 7 29.504818258743427 35.0 30.0 37.0 2.0 37.0 8 29.33271959549855 35.0 29.0 37.0 2.0 37.0 9 30.293664790711816 37.0 28.0 39.0 2.0 39.0 10-11 30.237068565631816 36.0 28.5 39.0 2.0 39.0 12-13 30.222333132285314 37.0 28.0 39.0 2.0 39.0 14-15 30.812927660336186 37.0 27.5 40.0 2.0 41.0 16-17 31.17568259152378 37.0 29.0 40.0 2.0 41.0 18-19 30.976870441804778 37.0 28.0 40.0 2.0 41.0 20-21 30.887842896512304 37.0 27.5 40.0 2.0 41.0 22-23 30.932256359360757 37.0 28.0 40.0 2.0 41.0 24-25 31.021859824272656 37.0 28.5 40.0 2.0 41.0 26-27 30.820900916353665 37.0 27.5 40.0 2.0 41.0 28-29 30.703138031656536 37.0 28.0 40.0 2.0 41.0 30-31 30.55994296187433 37.0 27.5 40.0 2.0 41.0 32-33 30.477333841522906 37.0 27.0 40.0 2.0 41.0 34-35 30.529968735124413 37.0 28.0 40.0 2.0 41.0 36-37 30.459593203751226 37.0 28.0 40.0 2.0 41.0 38-39 30.16576156109854 37.0 27.0 40.0 2.0 41.0 40-41 30.027446092685086 37.0 27.0 40.0 2.0 41.0 42-43 29.502179824796116 36.0 25.5 40.0 2.0 41.0 44-45 29.326124386953488 35.0 25.0 40.0 2.0 41.0 46-47 29.285880119451033 35.5 25.5 40.0 2.0 41.0 48-49 29.220502902031974 36.0 26.0 40.0 2.0 41.0 50-51 28.98052167170689 35.0 24.0 40.0 2.0 41.0 52-53 28.880624096364706 35.0 24.5 39.5 2.0 41.0 54-55 28.714876673280763 35.0 24.0 39.0 2.0 41.0 56-57 28.492199438078185 35.0 23.5 39.0 2.0 40.0 58-59 28.286008880542656 34.5 23.0 39.0 2.0 40.0 60-61 28.008217341244702 34.0 23.0 39.0 2.0 40.0 62-63 27.83236205562526 34.0 22.5 38.5 2.0 40.0 64-65 27.625100981645584 34.0 22.0 38.0 2.0 40.0 66-67 27.372853212589234 34.0 22.0 38.0 2.0 40.0 68-69 27.1413608668636 33.5 22.0 37.5 2.0 40.0 70-71 26.728292597545874 33.0 20.0 37.0 2.0 39.5 72-73 26.508538893444474 33.0 20.0 36.5 2.0 39.0 74-75 26.492063315871523 33.0 20.0 36.0 2.0 39.0 76-77 26.25151808392279 33.0 20.0 36.0 2.0 39.0 78-79 25.961670907681572 33.0 19.0 35.5 2.0 38.0 80-81 25.56672955941039 32.0 18.0 35.0 2.0 37.0 82-83 25.663561288760654 33.0 19.0 35.0 2.0 37.0 84-85 25.488843236993084 33.0 18.0 35.0 2.0 36.5 86-87 25.35817398077183 33.0 18.0 35.0 2.0 36.0 88-89 25.185958240744917 33.0 17.0 35.0 2.0 36.0 90-91 25.046508459556982 33.0 15.0 35.0 2.0 35.5 92-93 24.920929122277002 32.5 11.5 35.0 2.0 35.0 94-95 24.814497447328886 32.0 10.5 35.0 2.0 35.0 96-97 24.69666691047926 32.0 7.0 35.0 2.0 35.0 98-99 24.604110470006027 32.0 7.0 35.0 2.0 35.0 100-101 24.500831221783855 32.0 6.0 35.0 2.0 35.0 102-103 23.383754439745534 30.0 2.0 34.0 2.0 35.0 104-105 24.215887716589126 32.0 2.0 34.5 2.0 35.0 106-107 24.378023636423542 32.0 2.0 35.0 2.0 35.0 108-109 24.33892805339636 32.0 2.0 35.0 2.0 35.0 110-111 24.26912645530997 32.0 2.0 35.0 2.0 35.0 112-113 24.1814153811784 32.0 2.0 35.0 2.0 35.0 114-115 24.08215576913954 32.0 2.0 35.0 2.0 35.0 116-117 23.969697444150754 32.0 2.0 35.0 2.0 35.0 118-119 23.84425855227198 32.0 2.0 35.0 2.0 35.0 120-121 23.662025152579556 32.0 2.0 35.0 2.0 35.0 122-123 23.45693794439811 32.0 2.0 35.0 2.0 35.0 124-125 22.53169018445552 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2731050968018778 1101 2 1.6435834888909753 1101 3 1.1620025590647387 1101 4 1.2560893866722367 1101 5 1.1487812243626507 1101 6 1.0303451947567552 1101 7 1.0393993519529907 1101 8 1.226589972776022 1101 9 1.5924192108280337 1101 10-11 1.4693846155840191 1101 12-13 1.5108600750943033 1101 14-15 1.8484783434821956 1101 16-17 1.390808964722794 1101 18-19 1.4116276378202883 1101 20-21 1.6984663948884524 1101 22-23 1.4776290766366351 1101 24-25 1.3387997415806723 1101 26-27 1.6174662972107363 1101 28-29 1.6294493993471164 1101 30-31 1.7520636423957718 1101 32-33 1.7202023511835627 1101 34-35 1.5957898591619113 1101 36-37 1.4972053963155858 1101 38-39 1.3772759950955162 1101 40-41 1.4690610817679328 1101 42-43 1.5471569236048026 1101 44-45 1.415828828094849 1101 46-47 1.1595171139274854 1101 48-49 1.027508297578347 1101 50-51 1.0952697007020262 1101 52-53 0.9687776208835785 1101 54-55 1.0220335426928138 1101 56-57 1.0694914112903326 1101 58-59 0.8379844153346099 1101 60-61 0.8887471337025339 1101 62-63 0.7488389715719741 1101 64-65 0.6752673501089106 1101 66-67 0.6835721459512989 1101 68-69 0.6782595060487253 1101 70-71 0.6153916971172642 1101 72-73 0.555991512000908 1101 74-75 0.5487502507935567 1101 76-77 0.6769988243911094 1101 78-79 0.673734765760365 1101 80-81 0.7949834693929425 1101 82-83 0.8152990719421709 1101 84-85 0.8672118828612945 1101 86-87 0.8186880217255279 1101 88-89 0.82469441387925 1101 90-91 0.9293791457286176 1101 92-93 0.9217666249399734 1101 94-95 0.8672272244360428 1101 96-97 0.8252157194654579 1101 98-99 0.9165083971803085 1101 100-101 0.8586366461722967 1101 102-103 0.666381310113966 1101 104-105 0.8705906167108282 1101 106-107 0.8738368914859898 1101 108-109 0.8351181539335109 1101 110-111 0.8584743128715111 1101 112-113 0.808013689707284 1101 114-115 0.8513375267543779 1101 116-117 0.7807336594808767 1101 118-119 0.7474097904263495 1101 120-121 0.774658915305313 1101 122-123 0.7263615669764505 1101 124-125 0.618291219020545 1102 1 0.7485550196965498 1102 2 1.1564835873753054 1102 3 0.7927666437827767 1102 4 0.7204967996419107 1102 5 0.7517720414113107 1102 6 0.5297827465711329 1102 7 0.565746309423389 1102 8 0.4904809878013161 1102 9 1.0828272798764687 1102 10-11 1.0916063334153776 1102 12-13 1.2499066816077686 1102 14-15 1.401947046299039 1102 16-17 1.1547364312539372 1102 18-19 0.9295131323762149 1102 20-21 1.2324017316091123 1102 22-23 1.221829292768927 1102 24-25 0.8551606059773746 1102 26-27 1.3170061670484081 1102 28-29 0.9365398722988765 1102 30-31 0.9832969363116852 1102 32-33 1.2522282139620593 1102 34-35 1.069988973288801 1102 36-37 0.9401329134389087 1102 38-39 1.1802766598520549 1102 40-41 1.169716130798232 1102 42-43 1.3006476085167478 1102 44-45 0.8869737387116707 1102 46-47 0.7413727290777068 1102 48-49 0.7159031557598716 1102 50-51 0.8144107997676251 1102 52-53 0.6638217002537807 1102 54-55 0.5968847761931606 1102 56-57 0.6207379258870205 1102 58-59 0.6077891312264256 1102 60-61 0.688845286875388 1102 62-63 0.4666112658314461 1102 64-65 0.31724443047527373 1102 66-67 0.4148736534942117 1102 68-69 0.2948650088773057 1102 70-71 0.6195005770884237 1102 72-73 0.18779806627656015 1102 74-75 0.3364021726569 1102 76-77 0.16698470378356944 1102 78-79 0.2322099547939338 1102 80-81 0.39004631355359365 1102 82-83 0.4417435807004786 1102 84-85 0.4108521308639972 1102 86-87 0.4698642003860627 1102 88-89 0.32096563996214655 1102 90-91 0.4533601998409047 1102 92-93 0.5055836235872775 1102 94-95 0.37232098396966506 1102 96-97 0.2686685443640968 1102 98-99 0.4296727811681116 1102 100-101 0.38799100999404956 1102 102-103 0.28056015304600024 1102 104-105 0.3752210698700047 1102 106-107 0.37185405526248516 1102 108-109 0.4136457562682736 1102 110-111 0.40493249432860523 1102 112-113 0.26607196585127824 1102 114-115 0.39816587953814064 1102 116-117 0.4558858644597805 1102 118-119 0.4504224155310972 1102 120-121 0.5178364950766436 1102 122-123 0.3289562760500466 1102 124-125 0.22771594440087028 1103 1 0.8870515166258173 1103 2 1.2593688023597132 1103 3 0.878436102932465 1103 4 0.6399828667059779 1103 5 0.8104288715598251 1103 6 0.7086710656431059 1103 7 0.7140390441566922 1103 8 0.8957573452861283 1103 9 1.3386993605022006 1103 10-11 1.32998162029984 1103 12-13 1.2992872692231927 1103 14-15 1.5666698134802992 1103 16-17 1.177230191628368 1103 18-19 1.1691566148797747 1103 20-21 1.4139239089589957 1103 22-23 1.2274377835177823 1103 24-25 1.1148357828024018 1103 26-27 1.4099270053377673 1103 28-29 1.3056660016616988 1103 30-31 1.3462968767342396 1103 32-33 1.4653016951416227 1103 34-35 1.1932913643091148 1103 36-37 1.034611904764894 1103 38-39 1.0275721028421323 1103 40-41 1.177573975591212 1103 42-43 1.35226010214663 1103 44-45 1.0978975077812798 1103 46-47 0.9519820059653803 1103 48-49 0.7912789860747047 1103 50-51 0.8192764296564015 1103 52-53 0.5931658918657945 1103 54-55 0.6885289636557665 1103 56-57 0.603371613594561 1103 58-59 0.5743329741906393 1103 60-61 0.6540167347827754 1103 62-63 0.5389132005149158 1103 64-65 0.45232991091410213 1103 66-67 0.3941464939058825 1103 68-69 0.3119652875912493 1103 70-71 0.3834670406069165 1103 72-73 0.44828722789775455 1103 74-75 0.4234357915889042 1103 76-77 0.4051783503254889 1103 78-79 0.42585402857553945 1103 80-81 0.4961000809521998 1103 82-83 0.4640058003449745 1103 84-85 0.47715791818972164 1103 86-87 0.3729439877820653 1103 88-89 0.478348504912006 1103 90-91 0.5787445513433376 1103 92-93 0.5365373812746164 1103 94-95 0.42492488735293676 1103 96-97 0.49421590054040365 1103 98-99 0.4246974657314375 1103 100-101 0.3880442949082088 1103 102-103 0.3573635938561033 1103 104-105 0.41461148751962895 1103 106-107 0.519154121736058 1103 108-109 0.5560487301945152 1103 110-111 0.5076338818575081 1103 112-113 0.5617400575055349 1103 114-115 0.6013211211095353 1103 116-117 0.57243148155138 1103 118-119 0.6383400899599536 1103 120-121 0.6015513594734436 1103 122-123 0.4349661179117561 1103 124-125 0.32032000564726815 1104 1 1.13834308021341 1104 2 1.5325099078600566 1104 3 1.0476227906996733 1104 4 1.1517915433087396 1104 5 0.7732261740227315 1104 6 0.7788478458200778 1104 7 0.6196218176458181 1104 8 0.9487648290698054 1104 9 1.2219888179721643 1104 10-11 1.3443489498405867 1104 12-13 1.2635721434399017 1104 14-15 1.2106034928685538 1104 16-17 1.039736522075522 1104 18-19 1.1266970749840084 1104 20-21 1.4235908517306122 1104 22-23 1.334089295520922 1104 24-25 1.2572132054423832 1104 26-27 1.4885236135322693 1104 28-29 1.430427592117617 1104 30-31 1.4742395466430587 1104 32-33 1.432977417783949 1104 34-35 1.3180182670572549 1104 36-37 1.1975648043334814 1104 38-39 1.2896598524103098 1104 40-41 1.3250620379725184 1104 42-43 1.5088574375212005 1104 44-45 1.4388164182529941 1104 46-47 1.0953530873971644 1104 48-49 0.8944568943521176 1104 50-51 0.8158603927648684 1104 52-53 0.7299311195552427 1104 54-55 0.8265897784212193 1104 56-57 0.7872157267977151 1104 58-59 0.8939505370937155 1104 60-61 0.8670872535356615 1104 62-63 0.6734487490111434 1104 64-65 0.7301092414780044 1104 66-67 0.6186953108669648 1104 68-69 0.5966523287588217 1104 70-71 0.652504687888019 1104 72-73 0.6407471772846556 1104 74-75 0.5775238748447364 1104 76-77 0.5673726437714031 1104 78-79 0.6329471672374289 1104 80-81 0.7528454473696193 1104 82-83 0.7127464008570463 1104 84-85 0.7649883751494393 1104 86-87 0.664770816269467 1104 88-89 0.6265026093749029 1104 90-91 0.7001741934347585 1104 92-93 0.6693892070050964 1104 94-95 0.6255331626162999 1104 96-97 0.5998612309416558 1104 98-99 0.679238912804518 1104 100-101 0.7290830291501784 1104 102-103 0.6069205577860295 1104 104-105 0.6599602634935096 1104 106-107 0.6634701515614871 1104 108-109 0.6723702799603544 1104 110-111 0.623211824303624 1104 112-113 0.6264520595381349 1104 114-115 0.703253819242768 1104 116-117 0.667944001599782 1104 118-119 0.7042179275192062 1104 120-121 0.7258434159740723 1104 122-123 0.6670174789555112 1104 124-125 0.3913159585416892 1105 1 0.9960435889363453 1105 2 1.284071622261493 1105 3 0.7579094051174309 1105 4 0.8787342620509513 1105 5 0.8083311200518928 1105 6 0.6286880075712418 1105 7 0.5130647779577586 1105 8 0.6535526803339415 1105 9 0.9195716006216124 1105 10-11 0.8602054887567014 1105 12-13 0.9657013596268023 1105 14-15 1.2213228164358974 1105 16-17 0.7811460989940855 1105 18-19 0.8923182061851307 1105 20-21 0.8270427732740018 1105 22-23 0.9326013733059035 1105 24-25 0.6467412511502779 1105 26-27 0.9991003104017828 1105 28-29 1.1046206603525306 1105 30-31 1.069089188778431 1105 32-33 1.133001925535929 1105 34-35 0.8432868350702449 1105 36-37 0.5270590185864208 1105 38-39 0.7619315994154761 1105 40-41 0.9121980288691027 1105 42-43 0.9891219505301834 1105 44-45 0.7434782814314538 1105 46-47 0.23675119384592946 1105 48-49 0.3523265018866617 1105 50-51 0.41697667643554936 1105 52-53 0.24192584009905005 1105 54-55 0.23892825058738154 1105 56-57 0.4579784346985214 1105 58-59 0.47699490267266853 1105 60-61 0.3280061586003953 1105 62-63 0.34682156757566673 1105 64-65 0.09851838670467572 1105 66-67 0.0872099236140862 1105 68-69 -0.03670641655652318 1105 70-71 0.25178880663088066 1105 72-73 0.09415547362640453 1105 74-75 0.19733102596355678 1105 76-77 0.2726191501305557 1105 78-79 0.2852842433035967 1105 80-81 0.3906217851350853 1105 82-83 0.38326494326317473 1105 84-85 0.29856918688921397 1105 86-87 0.36949448668884344 1105 88-89 0.4274855269105302 1105 90-91 0.22883114524101345 1105 92-93 0.3072756893429123 1105 94-95 0.21977084758785992 1105 96-97 0.222280234926032 1105 98-99 0.3560790404132028 1105 100-101 0.40169182790084434 1105 102-103 0.20463173720288452 1105 104-105 0.2803671412854172 1105 106-107 0.33128909960832686 1105 108-109 0.34273775358996517 1105 110-111 0.3217269655576054 1105 112-113 0.23604727706547735 1105 114-115 0.2986170250625193 1105 116-117 0.31491245348157193 1105 118-119 0.31771264876457295 1105 120-121 0.2343775975063629 1105 122-123 0.20283723051987224 1105 124-125 0.20831641081261054 1106 1 0.21729718207908633 1106 2 -2.4594191444591615 1106 3 -0.500220595820096 1106 4 0.1611284092190708 1106 5 0.32275379555139594 1106 6 0.3425682216848287 1106 7 0.4647515102849802 1106 8 0.4798727034756638 1106 9 1.0461351178154104 1106 10-11 1.2137547313678247 1106 12-13 1.2217758957535167 1106 14-15 1.2624287013772566 1106 16-17 0.9057445043719028 1106 18-19 0.93240312403465 1106 20-21 1.2170384656809539 1106 22-23 1.0193024610332486 1106 24-25 1.1118262004796833 1106 26-27 1.0330071576431799 1106 28-29 1.0802986483688883 1106 30-31 1.2073150305133673 1106 32-33 1.304557845774827 1106 34-35 1.1237292178938105 1106 36-37 0.9200252903503241 1106 38-39 1.2187030200752318 1106 40-41 1.1937757595173615 1106 42-43 1.4277843553164118 1106 44-45 1.201705984486157 1106 46-47 1.0085430644116293 1106 48-49 0.8986524272207213 1106 50-51 0.9454481150789071 1106 52-53 0.9035849307974075 1106 54-55 0.7976315059624 1106 56-57 0.9109376297841791 1106 58-59 0.8737073838290961 1106 60-61 0.9519215191475361 1106 62-63 0.8786417219598945 1106 64-65 0.8399707335415805 1106 66-67 0.7517086945821241 1106 68-69 0.6331833248585355 1106 70-71 0.7185069324169504 1106 72-73 0.6496183269609297 1106 74-75 0.7197776838700385 1106 76-77 0.5858198222661777 1106 78-79 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-0.22388436534274092 2213 18-19 -0.17123564590018248 2213 20-21 0.04886567137085862 2213 22-23 0.025187254221453514 2213 24-25 -0.36209387507346236 2213 26-27 0.001066145899716986 2213 28-29 0.07679167608180393 2213 30-31 0.12458622333606684 2213 32-33 0.07289401966792042 2213 34-35 0.07375775383305694 2213 36-37 -0.16519830086430076 2213 38-39 0.076642043189878 2213 40-41 -0.11435250859640789 2213 42-43 0.28780858598135595 2213 44-45 0.24879000651970884 2213 46-47 -3.547759183528978 2213 48-49 -0.920199157634773 2213 50-51 -0.10125445760797902 2213 52-53 -0.15931438683727706 2213 54-55 -0.008075639673180035 2213 56-57 0.0022676098410130408 2213 58-59 0.03912325181975973 2213 60-61 0.16771742790611555 2213 62-63 0.003217248752797275 2213 64-65 0.03751568297805008 2213 66-67 -0.03431877297013486 2213 68-69 -0.019219075075270098 2213 70-71 0.13380923652297128 2213 72-73 -0.12657349507078663 2213 74-75 -0.27856862468051347 2213 76-77 -0.17539944083474168 2213 78-79 -0.1877464294241058 2213 80-81 0.023919189263008178 2213 82-83 -0.14794169124197865 2213 84-85 -0.1218691278488393 2213 86-87 -0.21565617947030802 2213 88-89 -0.1394480950166539 2213 90-91 -0.15042858520739344 2213 92-93 -0.10931376411519622 2213 94-95 -0.13857790681925053 2213 96-97 -0.18762208017614768 2213 98-99 -0.15444064940121294 2213 100-101 -0.08206413656293421 2213 102-103 -0.07763496876204812 2213 104-105 -0.15284031339626125 2213 106-107 -0.10386055137041694 2213 108-109 -0.1835579178146567 2213 110-111 -0.1760637229845976 2213 112-113 -0.1327259633145168 2213 114-115 -0.07961243114438332 2213 116-117 -0.10854725010360156 2213 118-119 -0.12482197111841842 2213 120-121 -0.026065797596050544 2213 122-123 0.08246675437571938 2213 124-125 0.001662148683660547 2214 1 -7.512959000301471 2214 2 -3.016435584598213 2214 3 -4.86712074354206 2214 4 -2.4794757056279124 2214 5 -1.5324675534070913 2214 6 -1.2710194839272795 2214 7 -1.0695104052618518 2214 8 -0.9870499159976767 2214 9 -1.080645647071794 2214 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-1.6689881975079395 2216 68-69 -1.645986471217764 2216 70-71 -3.8345868999793176 2216 72-73 -1.914186492240276 2216 74-75 -1.5375575513851167 2216 76-77 -1.3713548499270836 2216 78-79 -1.4315352296039414 2216 80-81 -1.3011078659320567 2216 82-83 -1.4584937766544748 2216 84-85 -1.3778329199557149 2216 86-87 -1.4893015523228712 2216 88-89 -1.4892568148315917 2216 90-91 -1.3917892443905053 2216 92-93 -1.3301968160856568 2216 94-95 -1.5150933809110043 2216 96-97 -1.5311287628930046 2216 98-99 -1.6097698710025945 2216 100-101 -1.7651979684787769 2216 102-103 -1.4496806982516723 2216 104-105 -1.487347543294863 2216 106-107 -1.5038427846999198 2216 108-109 -1.648114158883974 2216 110-111 -1.4855790963075357 2216 112-113 -1.6407219119498997 2216 114-115 -1.5661923629281205 2216 116-117 -1.4305155223710848 2216 118-119 -1.5289504582348954 2216 120-121 -1.7119132307834164 2216 122-123 -1.5403983163500747 2216 124-125 -1.3422834377389066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 465347.0 3 56855.0 4 32570.0 5 26046.0 6 26252.0 7 28554.0 8 31665.0 9 33248.0 10 34770.0 11 36612.0 12 37911.0 13 37990.0 14 37433.0 15 32117.0 16 27483.0 17 23922.0 18 22014.0 19 20860.0 20 20277.0 21 21767.0 22 26733.0 23 36665.0 24 50946.0 25 66212.0 26 76643.0 27 86746.0 28 99623.0 29 112723.0 30 130646.0 31 151634.0 32 181787.0 33 227348.0 34 294948.0 35 399953.0 36 556885.0 37 585468.0 38 140468.0 39 122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41464185763299 19.233058935116336 14.209276455227812 24.14302275202286 2 13.490953605379222 20.265563957336393 41.5796779729516 24.66380446433278 3 18.635284178666307 25.48101502309758 28.869527671036614 27.0141731271995 4 11.56649576656022 16.98382210763777 38.232326487563355 33.21735563823866 5 13.012557333762862 38.50939133072944 34.80179041474909 13.676260920758605 6 31.017815781780566 37.756124116425674 17.728009961995998 13.498050139797762 7 27.75643980489925 32.59280495734279 21.678677767097078 17.97207747066087 8 25.20753288947558 36.59367625642024 20.222013180847096 17.97677767325709 9 25.29050318446428 17.423001815110688 20.49248488910402 36.794010111321015 10-11 23.65679778049234 27.842983570995354 28.217383311495787 20.282835337016518 12-13 24.64641737784277 24.92622149038383 28.22403571754217 22.20332541423123 14-15 21.695500563456854 26.523797827980943 25.92300741964857 25.857694188913634 16-17 21.883105886230297 29.69362961242957 26.32620388887088 22.09706061246925 18-19 21.87477244838398 28.496615202637127 28.114878708988904 21.513733639989987 20-21 22.43068363196639 27.59604730914184 28.359497502790447 21.613771556101323 22-23 22.180766604848166 27.495901045747058 27.936654984027452 22.386677365377324 24-25 22.063969777128804 27.547601982170022 28.05856360344142 22.329864637259746 26-27 21.7924240109974 27.814160656170905 28.086835069872308 22.306580262959386 28-29 21.902215638189176 27.892703775751247 28.090287194756737 22.114793391302843 30-31 22.06816570041638 27.65252672304232 28.05258242774188 22.226725148799424 32-33 21.763781551918157 27.68083303947862 28.048010943102703 22.507374465500522 34-35 22.24405009678602 27.775042629499648 27.85160077559885 22.12930649811548 36-37 22.333044318709366 27.406661129787775 27.722899846895192 22.537394704607667 38-39 21.986387148449197 27.77307824591775 27.88611675041927 22.354417855213786 40-41 22.26681486995934 27.51271263193428 28.098976631519317 22.12149586658706 42-43 22.013217181351603 27.360767471009062 28.010613392407535 22.615401955231796 44-45 22.14881183203922 27.691726770236887 27.8190641318428 22.34039726588109 46-47 22.377939670557943 27.78826764972644 27.312876327569562 22.520916352146056 48-49 22.2391556376453 27.842170789476086 27.361292740261717 22.557380832616893 50-51 22.31881167584614 27.914683711478823 27.341818286516045 22.424686326158998 52-53 22.481879279954455 27.781615545146956 27.306227964786505 22.430277210112084 54-55 22.358597831916526 27.82841206742329 27.380241444914542 22.43274865574564 56-57 22.347681821544317 28.01637562047664 27.236642136321798 22.399300421657244 58-59 22.308296387986 27.690176533757032 27.296740325666697 22.70478675259027 60-61 22.204464337020944 27.908654406001666 27.475162269893094 22.4117189870843 62-63 22.195886317357783 27.88487474049447 27.625114693808346 22.294124248339404 64-65 22.15265909863463 27.771861453878905 27.47105529055287 22.604424156933593 66-67 22.141082271432946 27.98431701613811 27.285003635151956 22.58959707727699 68-69 22.44544133371882 27.70588414927723 27.447998428419307 22.40067608858464 70-71 22.359845033638408 27.604158317858424 27.46818657976145 22.56781006874172 72-73 22.364711206844618 27.75471587537378 27.22642693985809 22.654145977923505 74-75 22.258721072035986 27.970225017924154 27.218527560153987 22.552526349885866 76-77 22.361404028106772 27.669139942398786 27.403914612607565 22.565541416886877 78-79 22.388142308307522 27.692788880699364 27.353817491363653 22.56525131962946 80-81 22.359209259222634 27.93514715128973 27.214314192920913 22.491329396566726 82-83 22.42877685920238 27.83646237219015 27.300085803399277 22.434674965208195 84-85 22.27480659679989 27.89042932789938 27.26195595385821 22.572808121442524 86-87 22.34525190901879 27.953744406254838 27.24436259804928 22.456641086677095 88-89 22.423705196208708 27.943832830354122 27.173529116422383 22.458932857014787 90-91 22.30798639314241 27.90950496507067 27.301189166518537 22.481319475268382 92-93 22.407147833946805 27.845281613713553 27.366705994061334 22.380864558278308 94-95 22.170872660460944 27.76332803216704 27.477633912388306 22.588165394983708 96-97 22.453806594981778 27.571390552509197 27.441226746473628 22.5335761060354 98-99 22.254044719652548 27.727230809992502 27.30183424911597 22.71689022123898 100-101 22.15703641647985 27.64936794170368 27.550815053330652 22.642780588485824 102-103 22.127322467566337 27.43036518509744 27.712528343901475 22.729784003434744 104-105 21.962881877396754 27.663606249877148 27.53633328434919 22.837178588376904 106-107 22.043675435030817 27.64823014492876 27.40064069036739 22.907453729673037 108-109 22.088274647631856 27.52691310222286 27.364604809146748 23.020207440998536 110-111 22.06754386888529 27.67926314077703 27.42008018596968 22.833112804367993 112-113 22.032188604479256 27.723982968098582 27.607095993895488 22.636732433526674 114-115 21.99205179144685 27.807663787479413 27.38125443567146 22.819029985402274 116-117 21.88141030552814 27.807689693571092 27.544150280168555 22.766749720732214 118-119 21.828497146734314 27.744580292331513 27.430509947919667 22.996412613014503 120-121 21.77329317856203 27.903722113388497 27.491254641677145 22.831730066372327 122-123 21.78972868069686 27.84384794438961 27.459145223873143 22.907278151040387 124-125 21.875708112854458 27.684266099408195 27.446837358153697 22.99318842958365 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5059.0 1 3498.0 2 1478.0 3 929.5 4 772.5 5 711.0 6 768.5 7 886.5 8 1070.0 9 1206.0 10 1213.0 11 1232.5 12 1331.0 13 1503.5 14 1652.5 15 1840.5 16 2056.5 17 2332.0 18 2739.5 19 3242.0 20 3962.0 21 5062.5 22 6578.5 23 8563.5 24 11247.5 25 15015.5 26 20816.0 27 27898.0 28 34407.0 29 42068.0 30 52276.5 31 64261.0 32 77853.5 33 88863.0 34 98850.0 35 110037.5 36 121411.5 37 131620.0 38 137433.0 39 141929.0 40 145160.0 41 147979.0 42 150484.0 43 153290.0 44 157007.5 45 160188.5 46 160587.5 47 159790.0 48 159804.5 49 156780.0 50 151967.5 51 146201.0 52 139762.5 53 134465.0 54 128696.5 55 118487.0 56 107356.0 57 97309.0 58 88015.5 59 77312.5 60 64045.0 61 53398.5 62 43896.5 63 33165.0 64 24791.5 65 19438.0 66 14908.5 67 11460.5 68 9040.0 69 7170.5 70 5659.5 71 4336.5 72 3316.0 73 2699.0 74 2131.0 75 1604.5 76 1195.0 77 840.0 78 613.5 79 448.5 80 321.5 81 247.0 82 176.0 83 134.0 84 107.0 85 73.5 86 50.0 87 44.0 88 35.0 89 23.5 90 20.0 91 16.0 92 11.5 93 7.5 94 4.5 95 3.5 96 3.0 97 3.0 98 3.0 99 3.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2171645779405376 2 0.2563070150491571 3 0.30874619646512247 4 0.29486523667854336 5 0.32917036961911256 6 0.23880391929133263 7 0.2303912163903756 8 0.20928935328047507 9 0.20985020014053887 10-11 0.14964095285077292 12-13 0.17536980255619977 14-15 0.1936323784370273 16-17 0.16539140217089798 18-19 0.1924172102402224 20-21 0.18637642218495187 22-23 0.2090673513983665 24-25 0.2002690662811156 26-27 0.24768399457567614 28-29 0.1782908799523654 30-31 0.15687354048367902 32-33 0.1968806165015635 34-35 0.15595048002649067 36-37 0.1560906917415066 38-39 0.19003361108495123 40-41 0.2099086216884622 42-43 0.2714498802708797 44-45 0.17572033184373967 46-47 0.16049567645492438 48-49 0.16250537770348633 50-51 0.16848774421083357 52-53 0.1892040251044402 54-55 0.17629286301338812 56-57 0.17589559648750958 58-59 0.14903336875237044 60-61 0.16270401096642562 62-63 0.15080938380923917 64-65 0.14548133863863305 66-67 0.1330609175501368 68-69 0.13643768302010426 70-71 0.13854085874534353 72-73 0.1139804399983829 74-75 0.11645751363033134 76-77 0.1377696943127558 78-79 0.14414932734598151 80-81 0.15461846873383914 82-83 0.1562192191469379 84-85 0.14279394743416066 86-87 0.1702170220293636 88-89 0.1643281299986937 90-91 0.15293592815364773 92-93 0.15339161622744957 94-95 0.1762344414654648 96-97 0.16203800532009982 98-99 0.15794849696546795 100-101 0.1379449589565257 102-103 0.17762487430603965 104-105 0.1343929288427883 106-107 0.14114645978272325 108-109 0.15212971079230603 110-111 0.1268448648510963 112-113 0.11750910149295098 114-115 0.11559287472106632 116-117 0.11963564583735956 118-119 0.10292708313129213 120-121 0.09623197373928052 122-123 0.12370178557282212 124-125 0.11961227721819022 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4279243.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.270892811582456 #Duplication Level Percentage of deduplicated Percentage of total 1 79.71157417660358 48.839993172171816 2 10.864541039072014 13.313602589040402 3 4.086910725733974 7.512260070208589 4 1.8836025609501075 4.6164004244638495 5 1.0103235564817112 3.095171316710385 6 0.6179529694397227 2.27175180918842 7 0.3944539528328421 1.691798210418825 8 0.27670495876338547 1.3563167895059787 9 0.18551207765811825 1.0229841562900073 >10 0.8805970420635831 9.396161232423998 >50 0.05628840078538156 2.3734541680384904 >100 0.029494807517301925 3.1833383657070566 >500 0.001296983876240246 0.5699495271409831 >1k 7.467482220360731E-4 0.7568181686911784 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.673723833865008E-5 2 2.336861916932504E-5 0.0 0.0 0.0 4.673723833865008E-5 3 2.336861916932504E-5 0.0 0.0 0.0 4.673723833865008E-5 4 2.336861916932504E-5 0.0 0.0 0.0 4.673723833865008E-5 5 2.336861916932504E-5 0.0 0.0 2.336861916932504E-5 7.010585750797513E-5 6 4.673723833865008E-5 0.0 0.0 2.336861916932504E-5 9.347447667730016E-5 7 4.673723833865008E-5 0.0 0.0 2.336861916932504E-5 9.347447667730016E-5 8 4.673723833865008E-5 0.0 0.0 2.336861916932504E-5 9.347447667730016E-5 9 4.673723833865008E-5 0.0 0.0 2.336861916932504E-5 9.347447667730016E-5 10-11 4.673723833865008E-5 0.0 0.0 2.336861916932504E-5 1.4021171501595026E-4 12-13 9.347447667730016E-5 0.0 0.0 3.5052928753987565E-5 3.7389790670920066E-4 14-15 1.1684309584662521E-4 0.0 0.0 4.673723833865008E-5 4.440037642171758E-4 16-17 1.1684309584662521E-4 0.0 0.0 4.673723833865008E-5 5.14109621725151E-4 18-19 1.2852740543128774E-4 0.0 0.0 7.010585750797513E-5 5.14109621725151E-4 20-21 1.4021171501595026E-4 0.0 0.0 7.010585750797513E-5 6.192684079871136E-4 22-23 1.4021171501595026E-4 0.0 0.0 1.1684309584662521E-4 6.776899559104262E-4 24-25 1.8694895335460033E-4 0.0 0.0 1.8694895335460033E-4 6.776899559104262E-4 26-27 1.8694895335460033E-4 0.0 0.0 2.804234300319005E-4 7.127428846644137E-4 28-29 2.570548108625755E-4 0.0 0.0 6.192684079871136E-4 7.244271942490763E-4 30-31 3.0379204920122555E-4 0.0 0.0 0.002044754177315941 7.244271942490763E-4 32-33 3.0379204920122555E-4 0.0 0.0 0.004708776762618996 7.477958134184013E-4 34-35 3.1547635878588807E-4 0.0 0.0 0.00820238532843309 7.711644325877263E-4 36-37 3.271606683705506E-4 0.0 0.0 0.014371700789134901 7.711644325877263E-4 38-39 3.505292875398756E-4 0.0 0.0 0.02544842627539497 7.711644325877263E-4 40-41 3.505292875398756E-4 0.0 0.0 0.03982012706452987 7.711644325877263E-4 42-43 3.972665258785257E-4 0.0 0.0 0.05333887325398441 7.711644325877263E-4 44-45 4.206351450478508E-4 0.0 0.0 0.06917111274120213 8.29585980511039E-4 46-47 4.7905669297116336E-4 0.0 0.0 0.08876569991468117 8.880075284343516E-4 48-49 4.907410025558258E-4 0.0 0.0 0.11094251950637063 9.113761476036767E-4 50-51 4.907410025558258E-4 0.0 0.0 0.13626241837633432 0.0010048506242809768 52-53 4.907410025558258E-4 0.0 0.0 0.16075273126578699 0.001098325100958277 54-55 5.14109621725151E-4 1.168430958466252E-5 0.0 0.18809401569389728 0.001121693720127602 56-57 5.14109621725151E-4 3.5052928753987565E-5 0.0 0.21905743609325296 0.0011567466488815897 58-59 5.14109621725151E-4 4.673723833865008E-5 0.0 0.25425057656225647 0.0011684309584662521 60-61 5.14109621725151E-4 4.673723833865008E-5 0.0 0.2954143992290225 0.001191799577635577 62-63 5.257939313098134E-4 4.673723833865008E-5 0.0 0.337220858922945 0.0012385368159742271 64-65 5.60846860063801E-4 4.673723833865008E-5 0.0 0.38082670229290555 0.0012385368159742271 66-67 6.075840984024511E-4 4.673723833865008E-5 0.0 0.4322142958462513 0.0012619054351435523 68-69 6.309527175717761E-4 4.673723833865008E-5 0.0 0.4912784807967203 0.0012619054351435523 70-71 6.660056463257636E-4 4.673723833865008E-5 0.0 0.5574584102842488 0.0012619054351435523 72-73 7.244271942490763E-4 4.673723833865008E-5 0.0 0.6300413414241725 0.0013670642214055149 74-75 7.594801230030638E-4 4.673723833865008E-5 0.0 0.7050195560289518 0.0014839073172521402 76-77 7.711644325877263E-4 4.673723833865008E-5 0.0 0.7888544772989055 0.0015189602460061278 78-79 7.711644325877263E-4 4.673723833865008E-5 2.336861916932504E-5 0.8870727836675787 0.0015306445555907902 80-81 0.0010632721722042895 4.673723833865008E-5 2.336861916932504E-5 0.997746564053502 0.0015423288651754527 82-83 0.001098325100958277 4.673723833865008E-5 2.336861916932504E-5 1.116681151315782 0.0016241190322680904 84-85 0.001098325100958277 4.673723833865008E-5 2.336861916932504E-5 1.2395299822889236 0.0016358033418527528 86-87 0.001098325100958277 4.673723833865008E-5 2.336861916932504E-5 1.373151746699124 0.0017292778185300532 88-89 0.001098325100958277 4.673723833865008E-5 2.336861916932504E-5 1.5226758564540503 0.0018227522952073533 90-91 0.0012385368159742271 4.673723833865008E-5 2.336861916932504E-5 1.6964098556683975 0.0018227522952073533 92-93 0.0012385368159742271 5.8421547923312606E-5 2.336861916932504E-5 1.8812556332977586 0.0019395953910539784 94-95 0.0012385368159742271 7.010585750797513E-5 2.336861916932504E-5 2.0735560004421343 0.0019629640102233033 96-97 0.0012385368159742271 7.010585750797513E-5 2.336861916932504E-5 2.2745378096079145 0.002220018821085879 98-99 0.0013086426734822024 7.010585750797513E-5 2.336861916932504E-5 2.495336207829282 0.002383599155271154 100-101 0.0014138014597441652 7.010585750797513E-5 2.336861916932504E-5 2.7521222795714104 0.002383599155271154 102-103 0.0014955916268368026 7.010585750797513E-5 2.336861916932504E-5 3.0321718116966014 0.0024303363936098044 104-105 0.0017526464376993781 7.010585750797513E-5 2.336861916932504E-5 3.3166029599160414 0.0024770736319484543 106-107 0.0017993836760380282 7.010585750797513E-5 2.336861916932504E-5 3.609505232584361 0.0024770736319484543 108-109 0.0018110679856226906 7.010585750797513E-5 2.336861916932504E-5 3.9202728146076304 0.0025238108702871046 110-111 0.001857805223961341 7.010585750797513E-5 2.336861916932504E-5 4.268219869729295 0.0025238108702871046 112-113 0.0018928581527153283 7.010585750797513E-5 2.336861916932504E-5 4.652037755275875 0.0025705481086257544 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9550 0.0 42.1109 1 ATCAACG 10805 0.0 37.549244 3 TCAACGC 10800 0.0 37.290806 4 CAACGCA 10850 0.0 37.11983 5 AACGCAG 11290 0.0 34.743885 6 TATCAAC 12060 0.0 33.295948 2 GGTATCA 5785 0.0 32.13256 1 ACGCAGA 13195 0.0 29.2268 7 GTACATG 13450 0.0 29.014366 1 CGCAGAG 13110 0.0 27.41376 8 TACATGG 14115 0.0 26.760065 2 ACATGGG 13705 0.0 26.430378 3 CGCATAG 485 0.0 24.537062 8 GCAGAGT 15565 0.0 21.827858 9 AGAGTAC 12880 0.0 20.693207 10-11 GAGTACA 10875 0.0 20.380169 1 AACGCAT 730 0.0 19.5693 6 GGACCGA 1055 0.0 19.182861 6 GAGTACT 6715 0.0 19.137316 12-13 AGTACAT 10800 0.0 18.810896 2 >>END_MODULE