FastQCFastQC Report
Thu 26 May 2016
SRR1364640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364640_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4279243
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72040.1683475324958176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56500.13203269830668649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121600.045.184711
GGTATCA73950.044.241351
TCAACGC150250.036.0005044
ATCAACG150700.035.8131943
CAACGCA153350.034.8433465
TATCAAC160550.033.716922
AACGCAG160200.033.4532856
GTACATG140950.029.2150821
ACGCAGA190150.027.8722977
CGCAGAG189700.027.8128498
TACATGG146700.026.7921852
ACATGGG145300.025.7183483
GCAGAGT219100.023.238499
GGACCGA9800.023.0895946
AGAGTAC190600.022.11455710-11
GAGTACA116700.021.0387781
CATGGGG74650.020.8848154
GAGTACT127400.020.75137512-13
AGGACCG12450.020.0951445
AGTACTT142400.019.86172512-13
Produced by FastQC (version 0.11.5)