FastQCFastQC Report
Thu 26 May 2016
SRR1364639_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364639_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363962
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52380.38402829404338246No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13640.10000278600136954No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG1603.6379788E-1240.9071968
ACGCATA1655.456968E-1239.67057
GTATCAA51850.032.401881
TATCAAC60600.027.9200062
GGTATCA38050.027.4001661
ATCAACG59950.027.0302163
TCAACGC60000.026.8091034
CAACGCA61150.026.5007515
GTATACG900.009470616526.4781271
GTACATG41900.026.162161
AACGCAG60900.025.605826
GTTATCA7750.025.3677561
TACATGG40450.025.1853142
AACGCAT2601.1023076E-925.181066
GAGTACT26250.024.25255812-13
ACATGGG39650.024.040673
ACGCAGA65800.023.2416067
CGCAGAG64600.022.566318
GTACTTT29400.021.25010314-15
GTTCGCG2607.6900506E-720.5981457
Produced by FastQC (version 0.11.5)